








key: cord-271980-8x5g8r7c
authors: Yao, Ye; Pan, Jinhua; Liu, Zhixi; Meng, Xia; Wang, Weidong; Kan, Haidong; Wang, Weibing
title: Ambient nitrogen dioxide pollution and spread ability of COVID-19 in Chinese cities
date: 2020-09-30
journal: Ecotoxicol Environ Saf
DOI: 10.1016/j.ecoenv.2020.111421
sha: 
doc_id: 271980
cord_uid: 8x5g8r7c

This study aims to explore the relationship between ambient NO(2) levels and the transmission ability (basic reproductive number, R(0)) of COVID-19 in 63 Chinese cities. After adjustment for temperature and relative humidity, R(0) was positively associated with NO(2) concentration at city level. The temporal analysis within Hubei province indicated that all the 11 Hubei cities (except Xianning City) had significant positive correlations between NO(2) concentration (with 12-day time lag) and R(0) (r>0.51, p<0.005). Since the association between ambient NO(2) and R(0) indicated NO(2) may increase underlying risk of infection in the transmission process of COVID-19. In addition, NO(2) is also an indicator of traffic-related air pollution, the association between NO(2) and COVID-19’s spread ability suggest that reduced population movement may have reduced the spread of the SARS-CoV-2.

The COVID-19 pandemic has highlighted the importance of international solidarity and unity in the face of a dire global health and economic crisis. The pandemic, which was first reported in December 2019 in Wuhan, China, has caused 6,757,764 confirmed cases worldwide as of Jul 31, 2020, with 88,077 cases reported in China (NHC, 2020) . Although massive intervention measures (e.g., shutting down cities, extending holidays, and travel bans) have been implemented in China and many other countries, the spread of the disease is unlikely to be stopped worldwide in the near future. No effective vaccines or antiviral drugs have been clinically approved so far. Our current understanding of the factors that impact SARS-CoV-2 transmission is still limited.

Environmental factors are associated with the seasonality of respiratory-borne disease J o u r n a l P r e -p r o o f epidemics (Sooryanarain and Elankumaran, 2015) . Some research has investigated both indoor and outdoor environmental nitrogen dioxide (NO 2 ) pollution exposure to individuals (Salonen et al., 2019) . Previous cross-sectional and cohort research has provided evidence that ambient NO 2 exposure had longitudinal effects on growth in lung function (Molter et al., 2013) , causing pulmonary insufficiency (e.g., lung volume, expiratory flow). In addition, previous studies have suggested that ambient NO 2 exposure may play a role in the phenotypes of respiratory diseases including but not limited to influenza (Huang et al., 2016) , asthma (Weinmayr et al., 2010) , and severe acute respiratory syndrome (Kan et al., 2005) . For example, NO 2 might increase adults' susceptibility to viral infections (Goings et al., 1989) . Exposure to high levels of NO 2 before the start of a respiratory viral infection is associated with the severity of asthma exacerbation (Chauhan et al., 2003) .

Recently, a European study found that 78% of COVID-19 fatalities were located in five regions that showed the highest concentrations of NO 2 (Ogen, 2020) . This finding indicates that long-term NO 2 exposure may be an important risk factor for COVID-19 fatality.

However, Contini et al.(Contini and Costabile, 2020) discussed the relationships between atmospheric parameters and COVID-19 prevalence or fatality are influenced by several confounding factors, which made difficult to interpret correlations that are not indicating necessarily a cause-effect relationship in the description study. Although it's an inevitable limitation in our description study, our study aims to thoroughly explore the influence of NO 2 on COVID-19 transmission and to try to acquire more solid results with potential confounders adjusted.

In this study, we aim to assess the associations between ambient NO 2 levels and the spread ability of COVID-19 across 63 Chinese cities, and we provide information to facilitate the further prevention and control of COVID-19. J o u r n a l P r e -p r o o f 3 Methods

We collected COVID-19 confirmed case information reported by the National Health Commission of the People's Republic of China(WHO, 2020) and Health Commission of Hubei Province (http://wjw.hubei.gov.cn/bmdt/ztzl/fkxxgzbdgrfyyq/). Guidelines on the diagnosis and treatment of patients were defined according to the fourth version of the guidelines (issued on January 27, 2020). The clinical criteria for diagnosis were to meet any two of the three remaining clinical criteria (i.e., fever, radiographic findings of pneumonia, and normal or reduced white blood cell count or reduced lymphocyte count in the early stage of illness). An epidemiological criterion was added (e.g., linkage with a confirmed COVID-19 case) (NHC, 2020; Zhang et al., 2020) . The population movement in cities outside Hubei from the same period was obtained from Baidu Qianxi data (https://qianxi.baidu.com/2020/), and we used migration index and travel intensity to describe the movement. We obtained hourly concentrations of various air pollutants, including sulfur dioxide (SO 2 ), NO 2 , carbon monoxide (CO), ozone (O 3 ), fine particulate matter (PM 2.5 ), and inhalable particulate matter (PM 10 ). These data came from 63 cities (cities in China with more than 50 confirmed COVID-19 cases as of February 10, 2020) and ranged from January 1, 2020 to February 8, 2020. The data were acquired from the National Urban Air Quality 

The reproductive number (R 0 ), the average number of individuals infected by an initial infectious individual in a completely susceptible population, is fundamental to understanding disease transmission. We calculated R 0 for 63 Chinese cities with more than 50 cases as of February 10, 2020 (the COVID-19 peak period in China), including 12 and 51 cities inside and outside Hubei, respectively. We used the method introduced by Aaron et al. to estimate R 0 (Aaron A. King et al., 2017) . First, we constructed a linear regression model to estimate the relevant coefficient. Second, we obtained R 0 by combining the coefficients obtained from the previous step with the average incubation and confirmation periods. We assigned the average values of the incubation period and the mean course from case infection to confirmation as 7 and 3.8 days, respectively. These values were obtained in previous mathematical research (Pan et al., 2020) . All calculations were completed in R software version 3.6.1 (R Foundation for Statistical Computing).

Mediation is a hypothesized causal chain in which one variable affects a second variable that, in turn, affects a third variable (Lederer et al., 2019) . The relationship between NO 2 concentration and R 0 of COVID-19 may be mediated by population density or other air pollutants, such as city population and city area. Those mediators may indirectly affect the R 0 value of COVID-19 by modulating the NO 2 concentration, thus affecting the spread of COVID-19. In this study, we used mediation analysis to explore whether these factors were J o u r n a l P r e -p r o o f mediators of the relationship between NO 2 and R 0 of COVID-19, and we used bootstrapping to estimate standard error while testing the significance of these mediating effects.

We conducted a cross-sectional analysis to examine the associations of NO 2 with R 0 of COVID-19. We also conducted a longitudinal analysis to examine the temporal associations (with daily data points) of NO 2 with R 0 in cities inside Hubei Province since the date when they had enough confirmed cases to acquire stable daily R 0 values. The other covariates, including health policies, were quite similar throughout Hubei Province. When examining the correlation between NO 2 and R 0 of COVID-19, we estimated the associations of NO 2 concentration with R 0 both inside and outside Hubei province (r & p) in the same period by using multiple linear regression models after controlling for temperature and relative humidity (as covariates in the regression model) separately. Then, we used meta-analysis to pool the estimates of the specific associations of NO 2 concentration with R 0 (meta χ 2 & p).

We also examined the corresponding temporal associations between NO 2 and R 0 of COVID-19 across the different cities inside and outside Hubei Province using multiple linear regression models after controlling for temperature and relative humidity separately. The change of R 0 per 10 μg/m 3 increase in NO 2 pollution was calculated. Given that associations between NO 2 and COVID-19 prevalence are influenced by several confounding factors, we further examined the associations of NO 2 with the R 0 of COVID-19 with adjustment for density of population, GDP per capita and hospital beds per capita in the main model. 

Among the 63 investigated cities, the mean±standard deviation and range of NO 2 concentration and R 0 were (27.9±8.3 ug/m 3 , 10.7-53.0 ug/m 3 ) and (1.4±0.3, 0.6-2.5), respectively. The cities with the three highest R 0 values were Wuhan, Huanggang, and Yichang, which are all in Hubei Province. The similarity of the spatial distributions between R 0 and NO 2 suggests a relationship between R 0 and NO 2 concentration (Figure 1) . No matter Hubei Province or outside of Hubei Province, the daily concentration trend of NO 2 from January to march in 2016-2019 is almost the same, but it is obvious that the daily concentration of NO 2 in 2020 is lower than that in other years, especially after January 23, 2020 (Figure 2) , which may be due to the closure of Wuhan city in Hubei.

J o u r n a l P r e -p r o o f 

The scatter diagram of R 0 and NO 2 distributions (Figure 3) shows that R 0 tends to increase with NO 2 concentration, suggesting a positive correlation between R 0 and NO 2 concentration.

The cross-sectional analysis indicates that, after adjustment for temperature and relative humidity, R 0 was positively associated with NO 2 concentration at city level (meta χ 2 =10.18, J o u r n a l P r e -p r o o f p=0.037) (Figure 3) . Additionally, we further examined the associations of NO 2 with the R 0 of COVID-19 adjusted for density of population, GDP per capita, hospital beds per capita separately in the main model, and we found that none of the three covariate would affect the significant positive association between NO 2 with R 0 . In the following stratified analysis, a significant association was confirmed in cities outside Hubei (r=0.29, p=0.046), whereas the trend observed in cities inside Hubei was not significant (r=0.51, p=0.130) (Figure 3) . For every 10 μg/m 3 increase in NO 2 , R 0 increased by 0.12 (0.01-0.23) and 0.52 (−0.20 to 1.25), respectively. We did not find significant associations of temperature or relative humidity with R 0 of COVID-19 (meta χ 2 =4.62, p=0.370 and meta χ 2 =1.63, p=0.800, respectively).

The basic reproductive number R 0 was positively associated with NO 2 (meta χ 2 =10.18, p=0.037) in cities outside Hubei (blue points, 51 cities, r=0.29, p=0.046, solid line) and cities inside Hubei (green points, 12 cities, r=0.51, p=0.13, dashed line). We controlled the effects from temperature and relative humidity in the multiple linear regression models.

In addition, we found that R 0 was positively associated with the average NO 2 value from 2016-2019 (meta χ 2 =13.74, p=0.008; Figure 4a ) with adjustment for temperature and relative humidity. Because the average NO 2 value from 2016-2019 was significantly J o u r n a l P r e -p r o o f associated with that in early 2020 (r=0.85, p<0.0001), it is difficult to determine which factor is dominant in COVID-19 transmission. Moreover, the other investigated air pollutants (SO 2 , CO, O 3 , PM 2.5 , and PM 10 ) had no significant associations with R 0 (meta χ 2 <9.09, p>0.06;

Figure 4b-f). Furthermore, in order to avoid potential population movement effects in our study, which could decrease both NO 2 and R 0 , we collected reduced population movement data from 51 cities outside Hubei in the same period. We re-calculated NO 2 -R 0 associations including the population movement as a covariate, and we found that the NO 2 was still significantly correlated with R 0 of COVID-19 outside Hubei (r=0.32, p=0.024)

(a) The basic reproductive number R 0 was positively associated (meta χ 2 =13.74, p=0.0082) with the average NO 2 value from 2016-2019. (b)-(f) There were no significant associations between other air pollutants (SO 2 , CO, O 3 , PM 2.5 , and PM 10 ) and R 0 (meta χ 2 <9.09, p>0.06). We controlled the effects from temperature and relative humidity in the multiple linear regression models.

We calculated the daily R 0 values of 11 cities in Hubei (except Wuhan) from January 27 to February 26, 2020 (there were few COVID-19 confirmed cases in these cities afterwards) and normalized them based on Wuhan's daily R 0 value to eliminate the effects of other covariates.

We found that 11 Hubei cities (except Xianning City) had significantly positive correlations between NO 2 concentration (with 12-day time lag) and R 0 (r>0.51, p<0.005), suggesting a positive association between daily NO 2 concentration and COVID-19 spread ability on the temporal scale (Figure 5) . The same conclusion was reached for other time lag settings, but the most significant value was obtained with a delay of 12 days. The results of residual analysis and principal component analysis were shown in Figure S1 and Figure S2 , respectively.

Temporal correlation between NO 2 concentration and R 0 in 11 cities in Hubei. Except for Xianning, all of those cities had significant positive correlations (r>0.51, p<0.005) between NO 2 (with 12-day time lag) and daily R 0 (normalized based on Wuhan's daily R 0 ).

To eliminate the effects of city population and city area on the relationship between NO 2 concentration and R 0 value, we applied a mediation analysis to verify whether more densely populated cities had both greater R 0 and NO 2 concentration values. After adjustment for temperature and relative humidity, the mediation analysis found insignificant direct and indirect effects of city population and city area on R 0 (z=−1. 43, p=0.15 & z=−0.24, p=0.800 and z=−0.46, p=0.650 & z=1.15, p=0.250, respectively) . Thus, there were no apparent mediation effects between city population, city area, NO 2 , and R 0 . City population and city area did not influence the association between NO 2 concentration and R 0 .

This study explored the association between environmental factors and COVID-19 transmission. To our knowledge, little research has been done on the relationship between ambient air pollution and COVID-19 transmission. Our results show a significant association between NO 2 exposure and R 0 , suggesting that ambient NO 2 may contribute to the spread ability of COVID-19. To prevent city population and city area from affecting the relationship between NO 2 concentration and R 0 level, we applied a mediation analysis to verify whether more densely populated cities have both greater R 0 values and higher NO 2 concentrations.

The results showed that city population and city area did not influence the association between NO 2 concentration and R 0 level.

Although the closures of cities throughout Hubei occurred at approximately the same time point: the other cities of Hubei were locked down no longer than 1-2 days later than Wuhan City, the effect of the lockdown measure in different cities (e.g. cities with busy traffic vs. small rural cities) was not expected to have the same influence on the association between J o u r n a l P r e -p r o o f NO 2 and COVID-19 transmission. Multiple impact factors (the population density of the city, the typical road traffic and commercial exchanges, etc.) may still have confounded the association in the current analysis, but we have controlled for as many factors as possible to reduce confounding and solid our results, including the density of population, GDP per capita and hospital beds per capita. Previous studies also have suggested that the increased spread ability resulting from NO 2 exposure might be caused by the effects of NO 2 on host defenses that prevent viral spread (Becker and Soukup, 1999) . TM Chen et al. (Chen et al., 2007) found that exposure to NO 2 may harm to humans' health by interacting with the immune system; besides, IC Mills et al. (Mills et al., 2015) observed that short-term exposure to NO 2 had increased the hospital admission rates for a range of respiratory diseases in different age groups. Therefore, we speculated NO 2 have potential ability to contribute in the infection process of COVID-19 directly.

In addition, NO 2 emissions primarily come from burning fossil fuels (diesel, gasoline, coal), resulting in automobile and smokestack exhaust, the latter of which can be produced by electricity generation. Therefore, changes in NO 2 levels can be used to indicate changes in human activity and population movement due to the lockdown of cities. For example, we can see that since January 23, 2020, the daily average concentration of NO 2 after the closure of Wuhan is obviously lower than that of the same period in previous years ( Figure 2 ). Besides, it is well known that the spread of respiratory virus is through contact (direct or indirect via fomites) or through contaminated droplets emitted by cough, sneeze, respiration and speaking of infected individuals, both of which are related with human contact, social distance and population movement. Plus, NO 2 is as an indicator of traffic-related air pollution, the association between NO 2 and R 0 of COVID-19 may be explained by the relationship between viral spread and population movement. Of course, further investigations are warranted to provide additional details and illustrate this mechanism. J o u r n a l P r e -p r o o f Our study has some limitations: first, the averaging of NO 2 concentrations across cities likely resulted in an unknown degree of exposure misclassification, given the spatial variability and traffic-dependence of NO 2 and the potential for indoor exposure. Second, R 0 could be highly variable and is influenced by a variety of factors, including not only the previously mentioned mitigation efforts but also the comprehensiveness of case identification. Third, for the lack of corresponding data of NO, we did not explore the association between primary pollutant NO and the transmission ability of COVID-19. Given the ecological nature of this study, other city-level factors, such as the implementation ability of COVID-19 control policy, urbanization rate, and availability of medical resources, may affect the transmissibility of COVID-19 and confound our findings. Future studies should develop individual-based models with high spatial and temporal resolution to assess the correlations between air pollution and the epidemiologic characteristics of COVID-19. The mechanisms between NO 2 and the transmission of COVID-19 disease still require further research, besides, the spread of COVID-19 could be affected by many factors. We also believe that there is likely to have interaction of environmental factors and NPIs, which deserves further analysis. 

Introduction to model parameter estimation

Effect of nitrogen dioxide on respiratory viral infection in airway epithelial cells

Personal exposure to nitrogen dioxide (NO2) and the severity of virus-induced asthma in children

Outdoor air pollution: nitrogen dioxide, sulfur dioxide, and carbon monoxide health effects

Effect of nitrogen dioxide exposure on susceptibility to influenza A virus infection in healthy adults

Acute effects of air pollution on influenza-like illness in Nanjing, China: A population-based study

Relationship between ambient air pollution and daily mortality of SARS in Beijing

Control of Confounding and Reporting of Results in Causal Inference Studies. Guidance for Authors from Editors of Respiratory, Sleep, and Critical Care Journals

Quantitative systematic review of the associations between short-term exposure to nitrogen dioxide and mortality and hospital admissions

Long-term exposure to PM10 and NO2 in association with lung volume and airway resistance in the MAAS birth cohort

National Health Commission of the People's Republic of China.Diagnosis and treatment guideline on pneumonia infection with 2019 novel coronavirus

Assessing nitrogen dioxide (NO2) levels as a contributing factor to coronavirus (COVID-19) fatality

Effectiveness of control strategies for Coronavirus Disease 2019: a SEIR dynamic modeling study

Human exposure to NO2 in school and office indoor environments

Environmental role in influenza virus outbreaks

Short-term effects of PM10 and NO2 on respiratory health among children with asthma or asthma-like symptoms: a systematic review and meta-analysis

Coronavirus disease (COVID-19) Situation Report -121

Evolving epidemiology and transmission dynamics of coronavirus disease 2019 outside Hubei province, China: a descriptive and modelling study

The funders of the study had no role in study design, data collection, data analysis, data interpretation, or writing the report. The corresponding author had full access to all of the study's data and takes final responsibility for the decision to submit for publication.

The authors declare no competing interests. ☒The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: None

