Bibliography
This is an automatically generated bibliography describing the content of this study carrel.
- cord-000924-wwuqxx1r
- author: Yan, Fang
- title: Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine
- date: 2013-03-24
- words: 3781
- flesch: 45
- summary: The S1 protein is very important for inducing protective immunity and has been successfully used to construct IBV DNA vaccines [6, 9] . As for the choice of a booster, the inactivated vaccine is prepared from the whole virus particle, so the immune responses induced by inactivated IBV vaccines could respond to the whole virus.
- keywords: 16n; allantoic; antibody; avian; birds; boost; bronchitis; cellular; challenge; chickens; china; columns; day; days; differences; different; dna; fig; group; higher; ibv; ibvsx16; immune; inactivated; infectious; letters; lymphocytes; multivalent; number; old; plasmid; prime; proliferation; protection; protein; pvax1; responses; serum; significant; specific; study; titers; usa; vaccination; vaccine; virus
- versions: original; plain text
- cord-001083-vy1nxax2
- author: Malagnac, Fabienne
- title: Rab-GDI Complex Dissociation Factor Expressed through Translational Frameshifting in Filamentous Ascomycetes
- date: 2013-09-19
- words: 6838
- flesch: 52
- summary: The corrected version of the PaYIP3 CDS was PCR amplified using the PhusionH High-Fidelity DNA Polymerase, P. anserina PaYIP3 C The corrected in-frame form (PaYIP3 C -GFP) is more difficult to observe in the hyphae, as expected if it is present in lower amounts.
- keywords: -gfp; accuracy; allele; analysis; anserina; ascospore; c -gfp; cds; cerevisiae; cloning; codon; conserved; control; dna; downstream; effect; efficiency; end; event; evolution; expression; factor; fig; form; frameshifting; functional; fusion; gene; genetic; growth; ibv; kda; long; medium; mrna; mutant; orf; orfs; payip3; payip3 c; payip3 gene; payip3 pseudoknot; payip3 t; pcr; pezizomycotina; phenotype; phylogenetic; plasmid; podospora; podospora anserina; present; programmed; protein; pseudoknot; rab; reading; recoding; region; results; ribosomal; ring; role; sequence; short; single; site; slippage; slippery; slippery sequence; spacer; species; strain; structure; transformants; transformation; translation; trna; type; vector; wild; wood
- versions: original; plain text
- cord-001768-8vljd5cv
- author: Awad, Faez
- title: Protection conferred by live infectious bronchitis vaccine viruses against variant Middle East IS/885/00-like and IS/1494/06-like isolates in commercial broiler chicks
- date: 2015-09-09
- words: 3940
- flesch: 45
- summary: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or hemagglutination-inhibiting antibody, or induce chicken tracheal protection Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Cytotoxic T lymphocytes are critical in the control of infectious bronchitis virus in poultry Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Assessment of cross-immunity dm chickens to strains of avian infectious bronchitis virus using tracheal organ cultures Humoral antibody response and assessment of protection following primary vaccination of chicks with maternally derived antibody against avian infectious bronchitis virus Infectious bronchitis virus variants: a review of the history, current situation and control measures Avian infectious bronchitis: cross-protection studies using different Australian subtypes Avian infectious bronchitis vaccine (live) European Pharmacopoeia Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens Attenuation, safety, and efficacy of an infectious bronchitis virus GA98 serotype vaccine A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey Ankara Üniversitesi Veteriner Fakültesi Dergisi Evaluation of the hemagglutinationinhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination Challenge experiments to evaluate cross-protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Comparison of ciliary activity and virus recovery from tracheas of chickens and humoral immunity after inoculation with serotypes of avian infectious bronchitis virus Evaluation of the effectiveness of two infectious bronchitis virus vaccine programs for preventing disease caused by a California IBV field isolate Identification of a novel nephropathogenic infectious bronchitis virus in Israel Vaccination of chickens against a Belgian nephropathogenic strain of infectious bronchitis virus B1648 using attenuated homologous and heterologous strains Lack of correlation between infectivity, serologic response and challenge results in immunization with an avian infectious bronchitis vaccine Infectious bronchitis virus: Immunopathogenesis of infection in the chicken A simple method of estimating fifty per cent endpoints Ciliary activity: a criterion for associating resistance to infectious bronchitis virus infection with ELISA antibody titer Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes
- keywords: 793b; antibody; avian; birds; broiler; bronchitis; cent; challenge; chicks; ciliary; clinical; commercial; control; cr88; d14; day; days; different; gross; group; h120; high; ibv; iii; infectious; is/1494; is/885; is/885/00; isolates; kidney; lesions; like; live; old; programme; protection; significant; signs; strains; study; tracheal; vaccinated; vaccination; vaccine; variant; virulent; virus; viruses
- versions: original; plain text
- cord-002407-25cawzi0
- author: Nogales, Aitor
- title: Reverse Genetics Approaches for the Development of Influenza Vaccines
- date: 2016-12-22
- words: 11138
- flesch: 36
- summary: A virus hemagglutinin viral RNA are important for efficient packaging into budding virions Experimental approaches to study genome packaging of influenza A viruses Plasmid-only rescue of influenza A virus vaccine candidates Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza Attenuated influenza virus vaccines with modified NS1 proteins Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine Attenuation of equine influenza viruses through truncations of the NS1 protein Attenuation and immunogenicity in mice of temperature-sensitive influenza viruses expressing truncated NS1 proteins Immunogenicity and protection efficacy of replication-deficient influenza A viruses with altered NS1 genes Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry Development of a dual-protective live-attenuated vaccine against H5N1 and H9N2 avian influenza viruses by modifying the NS1 gene Canine influenza viruses with modified NS1 proteins for the development of live-attenuated vaccines Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus NS1-truncated live-attenuated virus vaccine provides robust protection to aged mice from viral challenge Influenza B virus NS1-truncated mutants: Live-attenuated vaccine approach Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach Development of live-attenuated arenavirus vaccines based on codon deoptimization Live attenuated influenza virus vaccines by computer-aided rational design Deliberate reduction of hemagglutinin and neuraminidase expression of influenza virus leads to an ultraprotective live vaccine in mice A replication-incompetent virus possessing an uncleavable hemagglutinin as an influenza vaccine Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity Characterization of a neuraminidase-deficient influenza A virus as a potential gene delivery vector and a live vaccine A novel bivalent vaccine based on a PB2-knockout influenza virus protects mice from pandemic H1N1 and highly pathogenic H5N1 virus challenges A replication-incompetent PB2-knockout influenza A virus vaccine vector Induction of CD8 T cell heterologous protection by a single dose of single-cycle infectious influenza virus An eight-segment swine influenza virus harboring H1 and H3 hemagglutinins is attenuated and protective against H1N1 and H3N2 subtypes in pigs A recombinant influenza virus vaccine expressing the f protein of respiratory syncytial virus To date, three types of influenza virus vaccines are approved by the Food and Drug Administration (FDA) for human use: recombinant viral HA, inactivated virus and live-attenuated viruses [65] [66]
- keywords: ability; addition; adults; ann; antigenic; antivirals; approaches; attenuation; avian; bm2; cells; cellular; challenge; channel; chicken; children; codon; cold; current; cycle; date; development; different; dna; drift; effectiveness; efficacy; efficient; eggs; expression; fda; figure; generation; genes; genetics; genome; growth; h1n1; h3n2; h5n1; hemagglutinin; host; human; iav; iavs; ibv; iiv; immune; immunity; immunogenic; immunogenicity; important; inactivated; infected; infections; influenza; influenza vaccines; influenza virus; laivs; like; live; mdv; mice; molecular; multiple; mutations; nabs; need; nep; neuraminidase; new; novel; ns1; packaging; pandemic; pb1; pb2; plasmid; polymerase; potential; presence; process; production; protective; proteins; reassortment; recombinant; replication; respiratory; response; responsible; reverse; reverse genetics; review; rna; safety; sciiavs; seasonal; segments; selection; similar; single; specific; strains; studies; subtypes; swine; temperature; time; transcription; treatment; type; vaccination; vaccine; viral; virion; virus; virus vaccine; viruses; vrna; years
- versions: original; plain text
- cord-003148-o7y3wygc
- author: Shirvani, Edris
- title: A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV
- date: 2018-08-10
- words: 8703
- flesch: 48
- summary: However chickens immunized with either commercial live attenuated IBV vaccine or rNDV expressing IBV S protein showed very low levels of tracheal viral load. In the third IBV protection experiment, we showed that rNDV expressing IBV S protein protects adult chickens against a higher dose of virulent IBV challenge.
- keywords: analysis; bronchitis; cells; challenge; chickens; clinical; codon; commercial; day; days; discharge; disease; dose; efficacy; eggs; eid; experiment; expression; fig; gene; group; ibv; ibv challenge; ibv protection; ibv strain; ibv vaccine; immunization; immunized; infected; infectious; kda; lane; level; live; load; mass-41; non; old; pbs; post; protection; protein; recombinant; results; rndv; route; s gene; s protein; samples; serum; shedding; signs; site; spf; strain; subunit; swab; tm&ct; tracheal; transcription; vaccine; viral; virulent; virulent ibv; virus
- versions: original; plain text
- cord-003208-lwirkob3
- author: Yan, Liping
- title: Novel protein chip for the detection of antibodies against infectious bronchitis virus
- date: 2018-09-17
- words: 3979
- flesch: 47
- summary: To further evaluate IBV nsp5 protein chip, 186 clinical serum samples were detected by the IBV nsp5 protein chip and the nsp5 ELISA antibody test kit. The agar gel precipitation test is used in IBV antibody detection; however, this method has low sensitivity.
- keywords: antibodies; antibody; antigen; assays; avian; bronchitis; buffer; chickens; cit; clinical; concentration; data; detection; diagnosis; diluted; dilution; disease; elisa; fig; high; ibv; idexx; infectious; ipdms; kit; methods; microarray; min; negative; nsp5; positive; protein; rapid; rdt; recombinant; reproducibility; results; samples; sensitivity; serum; serum samples; snr; specificity; study; test; time; virus
- versions: original; plain text
- cord-003334-ion97n4b
- author: De Silva Senapathi, Upasama
- title: The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens
- date: 2018-11-15
- words: 5831
- flesch: 47
- summary: Additionally, the authors saw a significant reduction in the IBV nuclear (N) gene mRNA expression in various embryonic tissues pre-treated with CpG ODNs, compared with the control, highlighting the value of CpG ODN treatment in IBV control. Thus, our objectives were to determine the protective effect of the in ovo delivery of CpG ODNs at ED 18 against IBV infection encountered post-hatch and, then, to investigate the mechanisms of protection.
- keywords: animal; avian; birds; bronchitis; canada; cd4; cells; chickens; cloacal; control; cpg; cpg odns; cytokine; data; day; delivery; differences; dna; dpi; eggs; epithelium; experiments; expression; figure; gene; genome; goat; group; hatch; ibv; ibv genome; ibv infected; ibv infection; ifn; immune; inc; induced; infected; infectious; innate; loads; lungs; macrophages; min; mrna; odn; odns; order; oro; ovo; ovo cpg; pbs; post; pre; protection; rate; recruitment; replication; respiratory; response; severe; significant; specific; strain; study; survival; swabs; t cells; test; tissues; trachea; usa; viral; virus
- versions: original; plain text
- cord-004680-u3cnsdl8
- author: Lin, Z.
- title: Typing of recent infectious bronchitis virus isolates causing nephritis in chicken
- date: 1991
- words: 861
- flesch: 50
- summary: Oligonucleotide fingerprinting of ribonucleic acids of infectious bronchitis virus strain Infectious avian nephrosis (ureamia) in Australia Occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the Netherlands Serological comparisons of strains of infectious bronchitis virus using plaque-purified isolants Molecular epidemiology of infectious bronchitis virus in the Netherlands A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism Etiology of an infectious nephritis-nephrosis syndrome of chickens Our present observation that the four new isolates used were different from the reference strains including current vaccine strains suggests that they were not derived from the live attenuated vaccine.
- keywords: amplified; bronchitis; cleavage; different; dna; fig; ibv; infectious; isolates; nephritis; new; restriction; sites; strains; vaccine; virus
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- cord-004810-g0y7ied0
- author: Lee, S. K.
- title: S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea
- date: 2003-11-13
- words: 3753
- flesch: 55
- summary: Korean IBV isolates showed amino acid sequence similarity between 61.8% (K446-01 and K161-02) and 96.1% (K281-01 and K210-02) with each other and they showed amino acid sequence similarity between 42.9% (K161-02 and GA980470) and 96.5% (K203-02 and KB8523) compared to non-Korean IBV strains. The whole S 1 gene of representative 9 of Korean IBV isolates was sequenced to further characterize the isolates.
- keywords: acid; amino; analysis; arg; avian; bronchitis; different; field; gene; glycoprotein; h120; ibv; ibv isolates; infectious; isolates; k069; k142; k161; k203; k210; k281; k446; k507; k774; km91; korean; korean ibv; non; nucleotide; patterns; pcr; phylogenetic; rflp; rna; sequence; serotype; similar; similarity; spike; strains; type; viral; virus
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- cord-006991-2q5ore6g
- author: Chi, X.
- title: Oral administration of tea saponins to relive oxidative stress and immune suppression in chickens
- date: 2017-06-15
- words: 5210
- flesch: 38
- summary: Oxidative stress and inability to improve function with repeated additions of adenosine diphosphate Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes Infectious bronchitis virus antibodies in tears and their relationship to immunity Role of Vitamin E in Counteracting Oxidative Stress in Poultry Inhibition of HMC-1 mast cell proliferation by vitamin E: involvement of the protein kinase B pathway Effects of administration of α-tocopherol and tocotrienols on serum lipids and liver HMG CoA reductase activity Detoxification of oxidative stress in glioma stem cells: mechanism, clinical relevance, and therapeutic development Evidence that shock-induced immune suppression is mediated by adrenal hormones and peripheral betaadrenergic receptors Enhancement of the immune responses to vaccination against foot-and-mouth Rhizoma Atractylodis Macrocephalae (RAM) key: cord-006991-2q5ore6g authors: Chi, X.; Bi, S.; Xu, W.; Zhang, Y.; Liang, S.; Hu, S. title: Oral administration of tea saponins to relive oxidative stress and immune suppression in chickens date: 2017-06-15 journal:
- keywords: acid; activities; activity; administration; analysis; antibody; antioxidant; aoc; assay; birds; bivalent; blood; bronchitis; carbonyl; cat; cells; chickens; control; cyclophosphamide; damage; days; different; disease; effect; et al; figure; ginseng; glutathione; groups; gsh; ibv; immune; immunization; immunosuppression; infectious; leaf; live; lymphocyte; mda; mean; mice; ndv; newcastle; oral; oxidative; oxidative stress; proliferative; radicals; responses; ros; samples; saponins; serum; sod; specific; stem; stress; study; tea; titers; tocopherol; total; treatment; vaccine; virus; vitamin; water
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- cord-007648-tm0hn0hz
- author: Mockett, A.P.Adrian
- title: Envelope proteins of avian infectious bronchitis virus: Purification and biological properties
- date: 2002-12-20
- words: 2219
- flesch: 52
- summary: Three different antigens were used for the EI,ISAs: spike protein, membrane protein and IB virus. The advantages of purifying viral proteins using affinity chromatography with monoclonal antibodies are discussed.
- keywords: affinity; antibodies; antibody; antisera; chicken; chromatography; concentrations; darbyshire; days; ibv; immunoadsorbent; infection; inoculation; kda; membrane; mockett; monoclonal; neutralizing; np40; nucleocapsid; pbs; proteins; purification; purified; rabbit; sera; spike; viral; virus
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- cord-009487-7xb4huyz
- author: GODWIN, IR
- title: Simple rapid method of rumen cannulation
- date: 2008-03-10
- words: 1302
- flesch: 59
- summary: This response is common following vaccination of chickens, whereas wild IBV stimulate higher antibody levels. The haemagglutination was inhibited by specific IBV antiserum, indicating IBV of sub-type B (Faragher 1987).
- keywords: abdominal; affected; allantoic; birds; cannula; chickens; days; disease; eggs; faragher; flock; ibv; incision; inoculation; method; muscles; neck; pigeons; racing; rumen; sheep; skin
- versions: original; plain text
- cord-010608-eaa2znom
- author: Butt, Salman L.
- title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples
- date: 2020-03-05
- words: 6858
- flesch: 45
- summary: Therefore, our aim was to create a single, amplicon-based protocol to sequence the IBV S1 gene and develop a sequence analysis workflow to identify IBV types from clinical swab samples. A universal S1 primer set, 27 tailed with the MinION universal adapter sequence of 22 nucleotides (underlined, Table 2 ) to allow barcoding of amplicons, was used for targeted amplification of the IBV S1 gene for IBV.
- keywords: accurate; amplicon; ampseq; analysis; assays; avian; bead; blast; briefly; bronchitis; cdna; classification; clinical; consensus; coronavirus; data; database; detected; detection; disease; dna; final; ga08; ga98; gene; genome; genotypes; giv; high; ibv; ibv genotypes; ibv rt; ibvs; identification; infectious; kit; lineage; method; minion; multiple; nanopore; new; number; pan; pcr; positive; presence; primer; protocol; qpcr; rapid; reaction; reads; real; results; reverse; rna; rtpcr; run; samples; sanger; sequence; sequencing; serotypes; set; single; specific; strains; swab; table; time; total; typing; virus; viruses
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- cord-017894-8iahlshj
- author: Loa, Chien Chang
- title: A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus
- date: 2015-09-10
- words: 1309
- flesch: 46
- summary: On the other hand, IBV-positive result is able to exclude the presence of TCoV. Coronaviral enteritis of turkeys (blue comb disease) Coronavirus immunogens Antigenic characterization of a turkey coronavirus identifi ed in poult enteritis and mortality syndrome-affected turkeys Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay Sequence analysis of the matrix/nucleocapsid gene region of turkey coronavirus Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3′ untranslated region identifi es the virus as a close relative of infectious bronchitis virus Detection of antibody to turkey coronavirus by antibodycapture enzyme-linked immunosorbent assay utilizing infectious bronchitis virus antigen Nucleocapsid protein gene sequence analysis reveals close genomic relationship between turkey coronavirus and avian infectious bronchitis virus Experimental bovine coronavirus in turkey poults and young chickens Antigenic and genomic relationships among turkey and bovine enteric coronaviruses Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus Complete sequences of 3′end coding region for structural protein genes of turkey coronavirus Comparison of 3′ end encoding regions of turkey coronavirus isolates from Indiana, North Carolina, and Minnesota Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction Redesign of primers and application of the reverse transcriptase-polymerase chain reaction and restriction fragment length polymorphism test to the DE072 strain of infectious bronchitis virus The protocol A multiplex polymerase chain reaction for differential detection of turkey coronavirus from chicken infectious bronchitis virus and bovine coronavirus outlined in this chapter had been successfully carried out in the authors' studies on molecular diagnostics and molecular virology of turkey coronavirus infection in turkeys. key: cord-017894-8iahlshj authors: Loa, Chien Chang; Wu, Ching Ching; Lin, Tsang Long title: A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus date: 2015-09-10 journal: Animal Coronaviruses DOI: 10.1007/978-1-4939-3414-0_12 sha: doc_id: 17894 cord_uid: 8iahlshj A multiplex polymerase chain reaction (PCR) method for differential detection of turkey coronavirus (TCoV), infectious bronchitis virus (IBV), and bovine coronavirus (BCoV) is presented in this chapter.
- keywords: analysis; assay; bcov; bovine; bronchitis; chain; coronavirus; corresponding; detection; gene; ibv; infectious; min; multiplex; nucleocapsid; pcr; polymerase; positive; product; protein; reaction; sequence; tcov; turkey
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- cord-022187-7c3wz6c6
- author: Liu, Shengwang
- title: A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China
- date: 2010-10-19
- words: 3529
- flesch: 51
- summary: Archives of Virolgy The aetiology of uraemia of chickens Detection of infectious bronchitis virus Molecular epizootiology of infectious bronchitis virus in Sweden indicating the involvement of a vaccine strain A new strain of infectious bronchitis virus infecting domestic fowl in Great Britain A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains Nucleotide sequence of the gene coding for the peplomer protein ( 0/spike protein) of infectious bronchitis virus, strain D274 Phylogeny of antigenic variants of avian coronavirus IBV Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis Identification and analysis of the Georgia 98 serotype, a new serotype of infectious bronchitis virus Sequence analysis of nephropathogenic infectious bronchitis virus strains of the Massachusetts genotype in Beijing Nephropathogenic avian infectious bronchitis viruses. The different passages of allantoic fluids containing IBV isolates were used in subsequent experiments (Table 1) .
- keywords: accession; acid; allantoic; amino; analysis; bronchitis; chickens; china; chinese; different; eggs; embryos; flocks; gene; genotype; h120; ibv; identity; infectious; inoculated; isolates; lhi10; massachusetts; nephropathogenic; new; non; nucleotide; number; old; passages; pcr; protein; provinces; s1 protein; s1oligo3; sequence; severe; signs; strains; study; table; vaccinated; virus; viruses
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- cord-022378-ovxmy1as
- author: Cook, Jane K.A.
- title: Coronaviridae
- date: 2009-05-15
- words: 4037
- flesch: 48
- summary: Th e IBV contains four structural proteins: a surface projection, known as the spike, a nucleoprotein, a membrane protein and a small membrane protein. In IBV infection of commercial layers or broiler breeders, respiratory signs may or may not be observed and the most common manifestation is the eff ect on egg production and egg quality.
- keywords: acid; aff; age; avian; birds; broilers; bronchitis; challenge; common; coronaviruses; data; diff; disease; eff; egg; eggs; erent; genome; host; ibv; ibvs; important; inactivated; infection; isolation; layers; live; material; membrane; methods; molecular; mortality; new; normal; number; oviduct; present; production; quality; respiratory; result; secondary; serotype; signs; small; specifi; strain; test; tract; transmission; vaccination; vaccine; variants; virus; weeks; young
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- cord-252048-ftbjsoup
- author: McKinley, Enid T.
- title: Attenuated live vaccine usage affects accurate measures of virus diversity and mutation rates in avian coronavirus infectious bronchitis virus
- date: 2011-04-22
- words: 6386
- flesch: 44
- summary: To examine sequence changes not only in spike but also over the entire length of the genome, we sequenced the full-length genomes of 5 Mass type, 4 Conn type and 2 CAL type IBV field viruses that were isolated over a 41, 25 and 8 year period respectively. It has been shown that vaccine usage can result in faster evolutionary rates in IBV field viruses (Lee and Jackwood, 2001; Wang Fig. 4 .
- keywords: amplification; analysis; avian; bronchitis; bronchitis virus; cal; cal99; cavanagh; changes; chickens; conn; coronavirus; data; different; disease; diversity; emergence; entire; et al; evidence; evolutionary; field; fig; gene; genetic; genome; glycoprotein; high; ibv; infectious; isolates; jackwood; length; live; mass; mass/06; mega4; method; min; mutation; new; nucleotide; passage; pathogenicity; pcr; period; phylogenetic; positive; primer; rates; reaction; recombination; regions; rna; selection; sequences; serotype; site; specific; spike; strains; study; substitutions; time; trees; type; type viruses; vaccine; viruses; year
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- cord-253695-tjdw2uta
- author: Winter, Christine
- title: Infection of the tracheal epithelium by infectious bronchitis virus is sialic acid dependent
- date: 2007-12-28
- words: 3710
- flesch: 49
- summary: Sialic acids were detected with lectins from Maackia amurensis (binding to a2,3 linked sialic acids) or Sambucus nigra agglutinin (binding to a2,6 linked sialic acids) labeled with digoxigenin (DIG Glycan Differentiation kit, Roche, Basel, Switzerland). The importance of sialic acid for IBV infection was evident from the delayed appearance of ciliostasis on desialylated TOCs.
- keywords: a2,3; acid; activity; aminopeptidase; anti; avian; beaudette; binding; bronchitis; cells; chicken; ciliary; ciliostasis; coronavirus; cultured; determinant; effect; enzyme; epithelium; fig; germany; ibv; incubation; infected; infection; influenza; kidney; m41; neuraminidase; primary; protein; receptor; respiratory; rings; sialic; sialic acid; staining; strains; surface; tocs; tracheal; treatment; virus; viruses
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- cord-255141-55ho9av4
- author: Abolnik, Celia
- title: Genomic and single nucleotide polymorphism analysis of infectious bronchitis coronavirus
- date: 2015-04-03
- words: 6285
- flesch: 43
- summary: For example, M together with the accessory proteins 4a, 4b and 5 were all found to prevent the synthesis of IFN-b through the inhibition of interferon promotor activation and IRF-3 function, thus influencing disease outcome (Yang et al., 2013) . functional role in the pathogenesis of IBV, the homolog in MERs-CoV is a potent interferon antagonist (Yang et al., 2013) .
- keywords: accessory; acid; amino; analysis; assembly; avian; binding; bronchitis; bronchitis virus; cavanagh; cell; chickens; consensus; coronavirus; cov; data; disease; diversity; et al; fig; frequency; gene; genetic; genome; genomic; high; host; hvrs; ibv; ifn; infectious; infectious bronchitis; interferon; length; level; like; mechanism; membrane; missense; mrna; mutations; order; orf; orfs; poultry; present; production; protein; receptor; recombination; region; replication; residues; respiratory; rna; roles; sars; secondary; sequence; serotype; silent; similar; single; site; snps; specific; spike; strain; structure; studies; study; subunit; transcription; utr; vaccine; variable; variation; viral; virulence; virus; years
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- cord-255619-5h3l6nh6
- author: Kuo, Shu-Ming
- title: Evolution of infectious bronchitis virus in Taiwan: Positively selected sites in the nucleocapsid protein and their effects on RNA-binding activity
- date: 2013-03-23
- words: 6332
- flesch: 47
- summary: 1D and E are representative, we included all the strains in Fig. 1B and constructed their Bayesian trees (Fig. S2) according to the two segments of N-terminal and C-terminal amino acid sequences of IBV N protein (Fig. 1C) . IBV N protein may be vulnerable to alteration under immunological pressure.
- keywords: aat; acid; addition; affinity; amino; analysis; avian; beaudette; binding; bronchitis; capacity; codeml; coronavirus; crossover; data; epitope; et al; evolution; fig; fitness; genes; high; ibv; ibv n; ibv strains; ibvs; immune; infectious; kuo; length; likelihood; models; n gene; n protein; new; ntd; nucleocapsid; phylogenetic; positions; positive; positive selection; present; pressure; program; protein; putative; recombinant; recombination; region; residues; results; rna; rna recombination; selection; sequence; sites; strains; structure; study; table; taiwan; terminal; test; thr; tp/64; trees; trs; tw ibv; tw n; type; variants; viral; viral rna; virus
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- cord-255870-gmq5zs2d
- author: Liu, Shengwang
- title: Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3
- date: 2008-04-15
- words: 5716
- flesch: 48
- summary: Given that IBV ORF 3a has an important function in infection (Hodgson et al., 2006) , we undertook the first experiment to investigate the potential effect of the mutations that occurred in IBV strains CK/CH/LSD/03I and CK/CH/LLN/ 98I, which resulted in a truncated 3a gene and absence of the 3a gene, respectively, on the growth ability of virus in vitro. These results and ours in the present study suggest that although the general gene order of IBV is 5′-S-3a, 3b, 3c-M-5a, 5b-N-3′ in the 3′-encoding regions, the absence of nonstructural protein genes was tolerated by the virus, as in other coronaviruses (De Haan et al., 2002) .
- keywords: ability; allantoic; analysis; avian; bronchitis; bronchitis virus; cavanagh; chickens; china; coronavirus; corresponding; different; embryos; essential; et al; fig; gene; groups; ibv; ibv strains; infectious; inoculated; inoculation; liu; lkq3/03; lln/98i; lsd/03i; mutation; normal; nucleotide; order; orf; orfs; pcr; phylogenetic; position; products; protein; recombination; regions; replication; results; rna; sequence; similar; strains; structural; study; table; viral; virus; viruses
- versions: original; plain text
- cord-256444-grw5s2pf
- author: Lai, Michael M.C.
- title: The Molecular Biology of Coronaviruses
- date: 1997-12-31
- words: 35274
- flesch: 43
- summary: This phenomenon is reminiscent of the RNA reassortment that occurs in RNA viruses with segmented RNA genomes. This structure places coronavirus in a unique position among RNA viruses because it takes on the characteristics of positive-, negative-and double-strand RNA viruses in morphology.
- keywords: 3clp; a59; acid; acting; activity; additional; amino; analysis; antibodies; antibody; assembly; avian; baric; bcv; binding; biology; bos et; bovine; bovine coronavirus; bronchitis; bronchitis virus; budding; cause; cavanagh; cells; cellular; certain; characterization; cis; clear; cleavage; cleaved; complex; conserved; consistent; contrast; copy; core; coronavirus; coronavirus mouse; coronavirus rna; coronavirus transcription; corresponding; culture; defective; deletions; dependent; di rna; different; different rna; discontinuous; domain; downstream; early; efficiency; encodes; encoding; end; entry; envelope; essential; esterase; et al; evidence; evolution; example; experimental; expressed; expression; factors; family; feline; fig; formation; frame; free; frequency; functional; fusion; gallagher et; gastroenteritis; gastroenteritis virus; gene; genetic; genome; genomic rna; glycoprotein; glycosylation; godet et; golgi; group; hcv; hcv-229e; helper; hepatitis virus; heterogeneity; high; hofmann et; host; human; ibv; identification; identity; infected; infection; infectivity; influenza; inhibited; interaction; internal; intracellular; jhm; kda; known; kooi et; lai; large; leader rna; leader sequence; lee et; leibowitz et; length; like; lines; locker et; luytjes et; mab; major; makino; mechanism; membrane; membrane protein; mhv; mhv rna; model; molecular; molecules; monoclonal; mouse; mouse hepatitis; mrna; multiple; murine; murine coronavirus; mutants; mutations; n protein; natural; nature; negative; neutralizing; new; nonstructural; nucleocapsid; nucleocapsid protein; nucleotide; number; oc43; orf; orfs; packaging; particles; peng et; polymerase; polypeptide; polyprotein; porcine; positive; possible; potential; presence; present; primary; processing; products; protease; protein; protein gene; protein sequence; protein synthesis; recent; receptor; recombinant; recombination; region; regulation; related; requirement; residues; resistant; respiratory; result; risco et; rna; rna polymerase; rna recombination; rna replication; rna sequence; rna synthesis; rna transcription; rnas; role; rossen et; s protein; sawicki; schultze et; second; section; sequence; sethna et; sialic; signal; similar; single; site; size; sized rna; small; species; specific; spike; spike protein; splicing; start; strains; strand rna; structure; studies; study; subgenomic; subgenomic rna; surface; system; target; temperature; template; template rna; terminal; terminus; tgev; tissue; transcribed; transcription; translation; transmissible; transport; type; ucuaa; uitro; vennema et; viral rna; virion; virus; virus infection; virus particles; virus rna; viruses; zhang et
- versions: original; plain text
- cord-256859-7ixegm72
- author: Liu, S. W.
- title: Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between 1995 and 2004
- date: 2006-01-09
- words: 5161
- flesch: 47
- summary: IBV strains have been isolated and identified since 1982 in China. The diversity of the pathogenicity of IBV strains was expected; although the primary tissue of IBV infection is the respiratory tract, some isolates can grow in nonrespiratory organs such as the kidney, the female reproductive tract, intestine, and spleen of chickens [2, 14, 33, 36] .
- keywords: acid; amino; analysis; arg; avian; bronchitis; bronchitis virus; chickens; china; chinese; chinese ibv; cleavage; coronavirus; different; evolutionary; field; fig; gene; genetic; genotype; glycoprotein; h120; ibv; ibv isolates; ibv strains; ibvs; iii; infectious; infectious bronchitis; isolates; k069; kidney; korean; mass; molecular; nephropathogenic; phe; phylogenetic; protein; recognition; region; residues; s1 protein; s1oligo5; sequences; serotype; site; spike; strains; study; subunit; table; taiwan; thr; vaccine; vii; virus; viruses; years
- versions: original; plain text
- cord-257064-iafm3pcc
- author: Kint, Joeri
- title: Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo
- date: 2014-12-18
- words: 1816
- flesch: 59
- summary: The method of 'right and wrong cases' ('constant stimuli') without Gauss's formulae Beitrag zur kollektiven behandlung pharmakologischer reihenversuche A simple method of estimating fifty percent endpoints Plaque formation by infectious bronchitis virus in chicken embryo kidney cell cultures Effect of serial embryo passage of an Arkansas-type avian infectious bronchitis virus isolate on clinical response, virus recovery, and immunity Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains Attenuation, safety, and efficacy of an infectious bronchitis virus GA98 serotype vaccine Growth kinetics of embryo-and organ-culture adapted Beaudette strain of infectious bronchitis virus in embryonated chicken eggs Replication of avian infectious bronchitis virus in African green monkey kidney cell line key: cord-257064-iafm3pcc authors: Kint, Joeri; Maier, Helena Jane; Jagt, Erik title: Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo date: 2014-12-18 journal: Coronaviruses DOI: 10.1007/978-1-4939-2438-7_9 sha: doc_id: 257064 cord_uid: iafm3pcc Quantification of the number of infectious viruses in a sample is a basic virological technique.
- keywords: agar; beaudette; bronchitis; cells; chicken; cpe; cultures; dilution; eggs; embryos; fig; ibv; infectious; method; monolayer; number; particles; plaques; plate; positive; primary; sample; serial; tcid; tenfold; titer; titration; virus
- versions: original; plain text
- cord-257999-apg4uhhq
- author: Ababneh, Mustafa
- title: Presence of Infectious Bronchitis Virus Strain CK/CH/LDL/97I in the Middle East
- date: 2012-04-11
- words: 2378
- flesch: 57
- summary: This variability makes the S1 gene an ideal target in molecular assays to type IBV strains by RT-PCR and sequencing. Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Pathogenicity and antigenicity of a new variant of Korean nephropathogenic infectious bronchitis virus Identification of a novel nephropathogenic infectious bronchitis virus in Israel A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and pathogenicity and evaluation of protection induced by Massachusetts serotype H120 vaccine against QX-like strains Evaluation of the protection conferred by commercial vaccines and attenuated heterologous isolates in China against the CK/CH/LDL/97I strain of infectious bronchitis coronavirus Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Emergence of a group 3 coronavirus through recombination Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Molecular epizootiology of infectious bronchitis 6 ISRN Veterinary Science virus in Sweden indicating the involvement of a vaccine strain Efficacy of live infectious bronchitis vaccines against a novel European genotype, Italy 02 Phylogeny.fr: robust phylogenetic analysis for the non-specialist Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area, China Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain Efficacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens This work was funded by a grant from the Deanship of Research at Jordan University of Science and Technology.
- keywords: amplification; analysis; bronchitis; chickens; china; east; gene; h120; ibv; identity; infectious; iraq; isolates; jordan; ldl/97i; like; middle; nested; nucleotide; pcr; phylogenetic; presence; protection; rna; saudi; sequence; strain; vaccine; variant; viral; virus
- versions: original; plain text
- cord-258379-v3lceirh
- author: Liu, D. X.
- title: Association of the infectious bronchitis virus 3c protein with the virion envelope
- date: 1991-12-31
- words: 2878
- flesch: 46
- summary: One of these, 3c, a 12.4K protein encoded by the third open reading frame of the tricistronic mRNA3 was clearly detectable and was found to cofractionate with virion envelope proteins on detergent disruption of virus particles. This calculation also assumes that 2. (a) lmmunoprecipitation of radiolabeled virion proteins with specific antisera.
- keywords: amino; antisera; associated; association; buffer; cells; encode; envelope; fig; fractions; gradient; hydrophobic; ibv; infected; lanes; membrane; orfs; polypeptide; present; protein; radiolabeled; relative; rna; sds; similar; small; spike; structural; sucrose; tne; virion; virus
- versions: original; plain text
- cord-259480-1tqfoecc
- author: Li, Huixin
- title: Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens
- date: 2016-03-02
- words: 6035
- flesch: 43
- summary: Chen et al. (2010) reported partial protection of chickens against a recombinant fowlpox virus expressing the IBV S1 protein with only 25% of birds showing clinical signs. Western blotting was performed to detect the expression of IBV proteins from the rDEVs (Han et al., 2013) .
- keywords: analysis; antibodies; antibody; avian; birds; bronchitis; bronchitis virus; cd4; cd8; cefs; challenge; chickens; clinical; clone-03; control; coronavirus; covalent; days; detection; dna; duck; egfp; enteritis; et al; fig; gene; glycoprotein; group; ibv; immune; infected; infectious; infectious bronchitis; ldl/091022; levels; liu; live; lymphocytes; mouse; pcr; post; protection; proteins; ratio; rdev; recombinant; responses; shedding; significant; signs; single; specific; strain; structural; study; table; titers; vaccinated; vaccination; vaccine; viral; virulent; virus; viruses; week
- versions: original; plain text
- cord-259505-7hiss0j3
- author: Kong, Qingming
- title: Proteomic analysis of purified coronavirus infectious bronchitis virus particles
- date: 2010-06-09
- words: 6930
- flesch: 38
- summary: + signalling to membrane dynamics Intracellular and extracellular roles of S100 proteins S100-annexin complexes: some insights from structural studies Recruitment of Hsp70 chaperones: a crucial part of viral survival strategies Synthesis and quality control of viral membrane proteins Association of HSP70 with the adenovirus type 5 fiber protein in infected HEp-2 cells Association of heat shock protein 70 with enterovirus capsid precursor P1 in infected human cells Vaccinia virus infection induces a stress response that leads to association of Hsp70 with viral proteins Increased expression of Hsp70 and co-localization with nuclear protein in cells infected with the Hantaan virus Uncoating ATPase is a member of the 70 kilodalton family of stress proteins Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase Two-dimensional blue native/SDS-PAGE analysis reveals heat shock protein chaperone machinery involved in hepatitis B virus production in HepG2.2.15 cells Selective inhibition of virus protein synthesis by prostaglandin A1: a translational block associated with HSP70 synthesis Evolutionary constraints on chaperone-mediated folding provide an antiviral approach refractory to development of drug resistance Molecular chaperone Hsp90 is important for vaccinia virus growth in cells Hsp90 inhibitors suppress HCV replication in replicon cells and humanized liver mice Hepatitis C virus RNA replication is regulated by FKBP8 and Hsp90 The cellular chaperone heat shock protein 90 facilitates Flock House virus RNA replication in Drosophila cells Identification of Hsp90 as a stimulatory host factor involved in influenza virus RNA synthesis Involvement of Hsp90 in assembly and nuclear import of influenza virus RNA polymerase subunits Antiviral activity and RNA polymerase degradation following Hsp90 inhibition in a range of negative strand viruses Herpes simplex virus type 1 DNA polymerase requires the mammalian chaperone hsp90 for proper localization to the nucleus Development and application of Hsp90 inhibitors Enhanced association of mutant triosephosphate isomerase to red cell membranes and to brain microtubules A glycolytic enzyme binding domain on tubulin Glycolytic enzyme-tubulin interactions: role of tubulin carboxy terminals Enolase, a cellular glycolytic enzyme, is required for efficient transcription of Sendai virus genome Specific interaction in vitro and in vivo of glyceraldehyde-3-phosphate dehydrogenase and LA protein with cis-acting RNAs of human parainfluenza virus type 3 Human hepatic glyceraldehyde-3-phosphate dehydrogenase binds to the poly(U) tract of the 3' noncoding region of hepatitis C virus genomic RNA Identification of glyceraldehyde-3-phosphate dehydrogenase as a cellular protein that binds to the hepatitis B virus posttranscriptional regulatory element Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interaction with 3' ends of Japanese encephalitis virus RNA and colocalization with the viral NS5 protein Functional significance of the interaction of hepatitis A virus RNA with glyceraldehyde 3-phosphate dehydrogenase (GAPDH): opposing effects of GAPDH and polypyrimidine tract binding protein on internal ribosome entry site function Specific phosphorylated forms of glyceraldehyde 3-phosphate dehydrogenase associate with human parainfluenza virus type 3 and inhibit viral transcription in vitro Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1 Apolipoprotein A-I activates Cdc42 signaling through the ABCA1 transporter Apolipoprotein A-I and its amphipathic helix peptide analogues inhibit human immunodeficiency virus-induced syncytium formation Immunoprecipitation, with an antiserum to ovalbumin, of protein NP from influenza A virus and of glycoprotein C from the herpes simplex type I virus Identification and characterization of tenp, a gene transiently expressed before overt cell differentiation during neurogenesis A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry Proteomic analysis of purified coronavirus infectious bronchitis virus particles Proteome Science The authors declare that they have no competing interests. Coronavirus avian infectious bronchitis virus Envelope glycoprotein interactions in coronavirus assembly Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells Coronavirus spike protein inhibits host cell translation by interaction with eIF3f Interaction of the coronavirus infectious bronchitis virus membrane protein with betaactin and its implication in virion assembly and budding Induction of apoptosis in murine coronavirusinfected 17Cl-1 cells Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer Host and viral proteins in the virion of Kaposi's sarcoma-associated herpesvirus Virion proteins of Kaposi's sarcomaassociated herpesvirus A mass spectrometry-based proteomic approach to study Marek's Disease Virus gene expression Proteins of purified Epstein-Barr virus Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome Identification of proteins associated with murine cytomegalovirus virions Vaccinia virus proteome: identification of proteins in vaccinia virus intracellular mature virion particles Krijnse Locker J: Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis Protein composition of the vaccinia virus mature virion Specific incorporation of heat shock protein 70 family members into primate lentiviral virions Cellular proteins bound to immunodeficiency viruses: implications for pathogenesis and vaccines Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages Proteomic analysis of human immunodeficiency virus using liquid chromatography/tandem mass spectrometry effectively distinguishes specific incorporated host proteins Identification of host proteins associated with retroviral vector particles by proteomic analysis of highly purified vector preparations Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion Identification of cellular interaction partners of the influenza virus ribonucleoprotein complex and polymerase complex using proteomic-based approaches Cellular proteins in influenza virus particles Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and onedimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis Plunder and stowaways: incorporation of cellular proteins by enveloped viruses Viral proteomics Interaction of epithelial ion channels with the actin-based cytoskeleton
- keywords: actin; analysis; annexin; antibody; assembly; binding; bronchitis; budding; buffer; cells; cellular; centrifugation; chaperone; chicken; control; coronavirus; cycle; cytoskeletal; day; dehydrogenase; density; dimensional; eggs; electrophoresis; enveloped; family; fig; function; fusion; gapdh; gel; gels; gradients; h52; hepatitis; host; host proteins; hsp90; human; ibv; identification; immunodeficiency; important; incorporated; infected; infectious; influenza; interaction; life; linear; major; mass; membrane; min; molecular; negative; old; page; particles; pbs; phosphate; polyacrylamide; present; proteins; proteomic; purified; replication; rna; roles; saline; samples; sds; small; specific; spectrometry; spf; structural; studies; study; sucrose; tenp; tne; total; transcription; tris; tubulin; type; vaccinia; viral; virions; virus; viruses
- versions: original; plain text
- cord-259738-yuqc6dk0
- author: Tang, Mengjun
- title: Enhancement of the immunogenicity of an infectious bronchitis virus DNA vaccine by a bicistronic plasmid encoding nucleocapsid protein and interleukin-2
- date: 2008-03-07
- words: 3851
- flesch: 43
- summary: These results demonstrated that bicistronic DNA vaccine is an effective approach to increase IBV DNA vaccine immunogenicity. These results demonstrated that bicistronic DNA vaccine is an effective approach to increase IBV DNA vaccine immunogenicity.
- keywords: antibody; antigen; bicistronic; blood; bronchitis; cd3; cd8; cells; challenge; chickens; control; day; dna; dsred; encoding; expression; gene; group; ibv; il-2; il2; immune; immunity; immunization; immunized; increase; infectious; interleukin-2; kidney; lymphocytes; monocistronic; nucleocapsid; pbs; pcr; pires; plasmid; positive; primer; protection; protein; recombinant; responses; results; samples; specific; total; vaccinated; vaccination; vaccine; vector; vero; virus
- versions: original; plain text
- cord-259959-qzd3hf8y
- author: Alhatami, Abdullah O.
- title: Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq
- date: 2020-07-16
- words: 2365
- flesch: 42
- summary: IBV vaccine strains can perform recombination with field strains, reversing virulence [16] . Therefore, sequencing the S1 gene of IBV strains detected in the field is critical for control programs and epidemiological purposes.
- keywords: analysis; anicon; antigen; authors; avian; birds; bronchitis; chickens; control; disease; field; fta; gene; genetic; gmbh; ibv; infected; infectious; iraq; isolate; labor; molecular; pcr; phylogenetic; poultry; rna; samples; sequences; sequencing; specific; strains; swabs; tracheal; vaccination; vaccine; variant; virus; work; world
- versions: original; plain text
- cord-260042-cs0wp99n
- author: Khan, Samiullah
- title: Genes involved in mitochondrial biogenesis and function may not show synchronised responses to mitochondria in shell gland of laying chickens under infectious bronchitis virus challenge
- date: 2019-04-01
- words: 6940
- flesch: 41
- summary: Similarly, increases in mitochondrial DNA (mtDNA) have been observed during myogenesis in rats [17] and in brown adipocytes of various mammals in response to low temperature [18] . Infectious bronchitis virus; mtDNA: mitochondrial DNA; Na + -K
- keywords: acid; activity; affected; age; analysis; atpase; australia; avian; biogenesis; birds; bronchitis; bronchitis virus; cells; challenge; chickens; coactivator; control; copy; count; data; different; division; dna; drp1; egg; eggs; epithelial; expression; extraction; fission; formation; function; genes; gland; groups; hens; higher; ibv; ibv t; infectious; isthmus; kit; level; lower; magnum; manufacturer; mean; membrane; mitochondrial; mtdna; muscle; nuclear; number; oviduct; pcr; pgc-1α; plasmid; point; production; protein; protocol; qpcr; quantitative; reaction; real; receptor; recombinant; related; relative; rna; role; rox; samples; sdha; segments; shell; shell formation; shell gland; significant; step; strain; study; sybr; table; time; tissue; total; transport; viral; virus; volume
- versions: original; plain text
- cord-260145-grz0fe9l
- author: Liu, Shengwang
- title: Altered pathogenicity, immunogenicity, tissue tropism and 3′-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
- date: 2009-07-23
- words: 7524
- flesch: 48
- summary: Episodic evolution mediates interspecies transfer of a murine coronavirus Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a q159L amino acid substitution in the spike protein Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism Pathogenesis of chimeric MHV4/MHV-A59 recombinant viruses: the murine coronavirus spike protein is a major determinant of neurovirulence Murine coronavirus spike glycoprotein mediates degree of viral spread, inflammation, and virus-induced immunopathology in the central nervous system Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus Enhanced virulence mediated by the murine coronavirus, mouse hepatitis virus strain JHM, is associated with a glycine at residue 310 of the spike glycoprotein Two amino acid changes at the Nterminus of transmissible gastroenteritis coronavirus spike protein result in the loss of enteric tropism Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2 Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Sequence analysis of nephropathogenic infectious bronchitis virus strains of the Massachusetts genotype in Beijing Molecular epidemiology of infectious bronchitis virus isolates from China and Southeast Asia Growth kinetics of embryo-and organ-culture adapted Beaudette strain of infectious bronchitis virus in embryonated chicken eggs Transmission of infectious bronchitis virus within vaccinated and unvaccinated groups of chickens Infectious bronchitis virus: S1 gene characteristics of vaccines used in China and efficacy of vaccination against heterologous strains from China Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas) Molecular epizootiology of avian infectious bronchitis in Russia New variant of IBV in Poland New genotype of infectious bronchitis virus in chickens in Scotland Chinese QX strain of infectious bronchitis virus isolated in the UK Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs Sequence changes of infectious bronchitis virus isolates in the 3 7.3 kb of the genome after attenuating passage in embryonated eggs Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence Comparison of four regions in the replicase gene of heterologous infectious bronchitis virus strains Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates Rapid selection in chickens of subpopulations within ArkDPI-derived infectious bronchitis virus vaccines Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo Single-aminoacid substitutions in open reading frame (ORF) 1b-nsp14 and ORF 2a proteins of the coronavirus mouse hepatitis virus are attenuating in mice The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3 genomic RNA Novel variation in the N protein of avian infectious bronchitis virus A comparative sequence ananlysis to revise the current taxonomy of the family coronaviridae The molecular biology of coronaviruses Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus Infectious bronchitis The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex Coronavirus avian infectious bronchitis virus Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus Infectious bronchitis viruses with a novel genomic organization Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3 A polycistronic mRNA specified by the coronavirus infectious bronchitis virus Identification of a new membrane associated polypeptide specified by the coronavirus infectious bronchitis virus Association of the infectious bronchitis virus 3c protein with the virion envelope Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication Emergence of a coronavirus infectious bronchitis virus mutant with a truncated 3b gene: functional characterization of the 3b protein in pathogenesis and replication Effect of serial embryo passage of an Arkansas-type avian infectious bronchitis virus isolate on clinical response, virus recovery, and immunity Attenuation, safety, and efficacy of an infectious bronchitis virus GA98 serotype vaccine Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: a review Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives The V5A13.1 envelope glycoprotein deletion mutant of mouse hepatitis virus type-4 is neuroattenuated by its reduced rate of spread in the central nervous system MHV-A59 fusion mutants are attenuated and display altered hepatotropism
- keywords: -utr; acid; addition; allantoic; amino; attenuated; attenuation; avian; birds; bronchitis; bronchitis virus; challenge; changes; chickens; chicks; china; clinical; control; coronavirus; days; eggs; embryos; fluid; gene; genome; group; ibv; ibv ck; immunogenicity; infectious; infectious bronchitis; inoculated; inoculation; kidney; lesions; lhlj/04v; negative; old; p110; p40; p70; passage; pathogenicity; pcr; positive; post; protein; region; replication; respiratory; rna; samples; sequence; serial; serum; signs; spf; spike; strain; study; subpopulations; substitutions; table; tissue; titers; trachea; tropism; type; viral; virus; viruses
- versions: original; plain text
- cord-260667-5aurua6o
- author: Falchieri, Marco
- title: Avian metapneumoviruses expressing Infectious Bronchitis virus genes are stable and induce protection
- date: 2013-05-24
- words: 3818
- flesch: 45
- summary: Predictive computational comparison of S1 populations S1 genes with and without the deletion were compared. While virus AvF containing N gene alone or N + S1 genes together protected tracheas better than AvF containing the S1 gene alone, the expression of the inserted genes would need to be quantified, before conclusions about the relative contribution of each gene to protection, could be drawn.
- keywords: ampv; analysis; antibody; avf; avian; birds; bronchitis; cell; challenge; chickens; cilial; cloning; control; deletion; dpc; experiment; expression; eyedrop; field; fls; foreign; genes; genome; gfp; greater; group; ibv; induced; infectious; live; metapneumovirus; neg; observed; pcr; post; protection; protein; real; recombinant; replication; respiratory; sections; sequence; sites; start; stop; study; subtype; table; time; tracheal; tract; vaccination; vaccine; vero; virus; viruses
- versions: original; plain text
- cord-260799-kx6hfpu0
- author: Mahmood, Zana H.
- title: Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East
- date: 2011-05-12
- words: 3510
- flesch: 42
- summary: Classical and Variant Strains A 'novel' infectious bronchitis strain infecting broiler chickens in Italy Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41 Infectious bronchitis Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs Infectious bronchitis virus: immunopathogenesis of infection in the chicken Antigenic and molecular characterization of isolates of the Italy 02 infectious bronchitis virus genotype The isolation and characterization of six avian infectious bronchitis virus isolated in Morocco Molecular epizootiology of infectious bronchitis virus in Sweden indicating the involvement of a vaccine strain Genetically diverse coronaviruses in wild bird populations of north England Isolation of a variant infectious bronchitis virus in Australia that further illustrates diversity among emerging strains Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during Identification of a novel nephropathogenic infectious bronchitis virus in Israel Isolation and identification of Infectious bronchitis virus from commercial chickens Broadly targeted multiprobe QPCR for detection of coronaviruses: coronavirus is common among mallard ducks (Anas Platyrhynchos) Seroprevalence study of infectious bronchitis among poultry in Tamilnadu Serological studies of infectious bronchitis vaccines against Japanese field isolates of homologous and heterologous genotypes Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus A reverse transcriptasepolymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from This work was carried out with financial support from Kurdistan Institute for strategic studies and scientific researches, University of Sulaimani, and Directorate of Veterinary in Sulaimani, Iraq. S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction Molecular epizootiology of avian infectious bronchitis in Russia Sequence of the membrane protein gene from avian coronavirus IBV Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus Infectious Bronchitis Virus:
- keywords: acid; amino; amplification; analysis; avian; birds; broiler; bronchitis; different; disease; egypt; farms; gene; genotype; h120; ibv; infectious; iraq; isolates; isolation; israel; kidney; lesions; ma5; mixture; nephropathogenic; new; pcr; poultry; protein; reaction; region; sequence; spike; strains; subunit; sul/01/09; table; trachea; vaccinated; vaccine; virus
- versions: original; plain text
- cord-261388-d56ci0hl
- author: Tibbles, K.W
- title: Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus
- date: 1999-05-28
- words: 3915
- flesch: 43
- summary: The 3CLP domain was first identified as a possible component of IBV polyproteins (Gorbalenya et al., 1989) due to sequence similarity to picornavirus 3C proteinases (reviewed in Ryan and Flint, 1997) . A simple method for displaying the hydropathic character of a protein Screening for membrane-permeabilizing mutants of the poliovirus protein 3AB The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: Evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products Identification, expression, and processing of an 87-kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: identification of a 10-kilodalton polypeptide and determination of its cleavage sites Intracellular and in vitro-translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59 Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59 Recombination of protein domains facilitated by co-translational folding in eukaryotes Identification of a 24-kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites Virus-encoded proteinases of the picornavirus super-group Expression and characterization of a recombinant murine coronavirus 3C-like proteinase Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system Use of T7 RNA polymerase to direct expression of cloned genes Characterization in vitro of an autocatalytic processing activity associated with the predicted 3C-like proteinase domain of the coronavirus avian infectious bronchitis virus A region of the infectious bronchitis virus 1a polyprotein encoding the 3C-like protease domain is subject to rapid turnover when expressed in rabbit reticulocyte lysate Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity Biosynthesis, purification, and characterization of the human coronavirus 229E 3C-like proteinase
- keywords: 229e; 3clpro; activity; bacterial; bronchitis; cells; characterization; cleavage; coronavirus; data; domain; et al; expression; fragment; fusion; gene; hcv; human; ibv; identification; infectious; kda; lane; like; like proteinase; liu; maturation; mhv; orf1; plasmid; polymerase; polypeptide; polyprotein; presence; processing; product; proteinase; purification; purified; region; sequence; sites; studies; tag; target; terminal; terminus; tibbles; trans
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- cord-262226-7kwkla73
- author: Fang, Shouguo
- title: Identification of two ATR-dependent phosphorylation sites on coronavirus nucleocapsid protein with nonessential functions in viral replication and infectivity in cultured cells
- date: 2013-07-09
- words: 5649
- flesch: 43
- summary: Similar to other coronaviruses, IBV N protein is heavily phosphorylated. By using mass spectroscopic analysis, two conserved amino acid clusters, Ser190Ser192 and Thr378Ser379, in IBV N protein were identified as the two regions for phosphorylation (Chen et al., 2005) .
- keywords: acute; addition; ala; analysis; antibodies; atm; atr; binding; blot; bronchitis; cells; cellular; characterization; coronavirus; cultured; cycle; dependent; dna; et al; fig; growth; host; ibv; ibv n; infected; infection; kinase; kinetics; length; mutant; n protein; nucleocapsid; pchk1; peak; phosphorylation; positions; post; presence; protein; recombinant; replication; residues; respiratory; responsible; rna; schb; ser379; severe; similar; sites; specific; study; substitutions; substrates; syndrome; thr378; transcription; type; vero; viral; virus; viruses; western; wild
- versions: original; plain text
- cord-262940-eyejnexx
- author: Liu, Genmei
- title: Assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus M and S proteins
- date: 2013-11-12
- words: 3868
- flesch: 44
- summary: Here we report that transfection of Sf9 cells with a single recombinant baculovirus encoding M and S proteins resulted in the assembly of IBV VLPs; this is the first report that S protein plus M protein alone were able to be assembled into VLPs for coronaviruses. Interestingly, when M protein was expressed alone, it could be detected in the sedimentable particles of the culture media ((A), lane 2), but S protein could not be detected when it was expressed alone ((B), lane 2).
- keywords: antibodies; antibody; assembly; avian; bronchitis; cells; cellular; chickens; coronavirus; culture; day; fig; formation; groups; h120; higher; ibv; igg; immune; immunization; inactivated; infected; infectious; interactions; like; mice; min; neutralizing; particles; pbs; primary; protein; recombinant; responses; results; sedimentable; sera; serum; sf9; specific; structural; sucrose; titers; vaccine; viral; virus; vlps
- versions: original; plain text
- cord-263178-lvxxdvas
- author: Shan, Dan
- title: Effects of hypervariable regions in spike protein on pathogenicity, tropism, and serotypes of infectious bronchitis virus
- date: 2018-05-02
- words: 6895
- flesch: 44
- summary: The pathogenesis of virulent and avirulent avian infectious bronchitis virus Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein Recombinant infectious bronchitis coronavirus beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus Development and validation of RT-PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gammacoronaviruses within clinical samples Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Analysis of an immunodominant region of infectious bronchitis virus Antigenicity of the peplomer protein of infectious bronchitis virus Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens Identification of a trypsin-like serine proteinase domain encoded by ORF 1a of the coronavirus IBV Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products Infectious bronchitis virus: S1 gene characteristics of vaccines used in China and efficacy of vaccination against heterologous strains from China Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Identification of a newly isolated avian infectious bronchitis coronavirus variant in China exhibiting affinity for the respiratory tract Molecular characterization and pathogenicity of infectious bronchitis coronaviruses: complicated evolution and epidemiology in china caused by cocirculation of multiple types of infectious bronchitis coronaviruses Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3' untranslated region Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Identification of amino acids involved in a serotype and neutralization specific epitope within the s1 subunit of avian infectious bronchitis virus Epitopes on the peplomer protein of infectious bronchitis virus strain M41 as defined by monoclonal antibodies Multiple regions of the murine coronavirus spike glycoprotein influence neurovirulence Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus Assessment of molecular and genetic evolution, antigenicity and virulence properties during the persistence of the infectious bronchitis virus in broiler breeders Development and immunogenicity of recombinant GapA(+) Mycoplasma gallisepticum vaccine strain ts-11 expressing infectious bronchitis virus-S1 glycoprotein and chicken interleukin-6 Induction of protective immunity in chickens vaccinated with infectious bronchitis virus S1 glycoprotein expressed by a recombinant baculovirus Singleamino-acid substitutions in open reading frame (ORF) 1b-nsp14 and ORF 2a proteins of the coronavirus mouse hepatitis virus are attenuating in mice Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity Evidence of circulation of a Chinese strain of infectious bronchitis virus (QXIBV) in Italy Molecular epizootiology of avian infectious bronchitis in Russia Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Coronavirus IBV: removal of spike glycopolypeptide S1 by urea abolishes infectivity and haemagglutination but not attachment to cells Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China Evidence for a coiled-coil structure in the spike proteins of coronaviruses Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination Mouse hepatitis virus liver pathology is dependent on ADP-ribose-10phosphatase, a viral function conserved in the alpha-like supergroup Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cells An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells Functional and genetic studies of the substrate specificity of coronavirus infectious bronchitis virus 3C-like proteinase Coronavirus-induced ER stress response and its involvement in regulation of coronavirus-host interactions
- keywords: adsorption; amino; antigenic; avian; beaudette; beaudette strain; binding; birds; bronchitis; bronchitis virus; celisa; cells; challenge; chickens; china; clinical; coronavirus; cultured; days; differences; eggs; eid; et al; fig; functions; gene; glycoprotein; group; heterogenous; hvrs; ibvs; iii; infected; infectious; infectious bronchitis; kidneys; ldl/091022; like; multiple; neutralization; pathogenicity; pbs; pcr; protection; protein; real; recombinant; recombinant ibvs; replicates; results; rhvr; rna; samples; scores; serotype; spf; spike; strain; study; subunit; table; tests; time; trachea; vero; vero cells; viral; virus; viruses
- versions: original; plain text
- cord-263193-paeosfiu
- author: Zhu, Jinyan
- title: Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
- date: 2020-06-10
- words: 2847
- flesch: 49
- summary: Importantly, we found significant activation of the TLR3-TRIF pathway and inhibition of the TLR7-MYD88 pathway at 48 hpi by different IBV strains in CEK cells. The effect of agonists and inhibitors on the replication of different IBV strains.
- keywords: activation; bronchitis; bx795; cek; celastrol; cells; chickens; chloroquine; coronavirus; different; expression; fig; h52; higher; hpi; ibv; immune; infected; infectious; inhibitor; innate; like; mda5; molecules; myd88; pathway; poly(i; receptor; recognition; replication; response; rna; strain; tlr3; tlr7; toll; treatment; trif; virus
- versions: original; plain text
- cord-263476-ju7xqwa7
- author: Xia, Jing
- title: Phylogenetic and antigenic analysis of avian infectious bronchitis virus in southwestern China, 2012–2016
- date: 2016-08-13
- words: 5711
- flesch: 43
- summary: infectious bronchitis virus isolates in southern China Sequence analysis of infectious bronchitis virus isolates from the 1960s in the United States Dissecting RNA recombination in vitro: role of RNA sequences and the viral replicase Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran Isolation and molecular characterization of nephropathic infectious bronchitis virus isolates of Gujarat state Analysis of the S1 gene of the avian infectious bronchitis virus (IBV) reveals changes in the IBV genetic groups circulating in southern Thailand Genotypes and serotypes of avian infectious bronchitis viruses isolated during A simple method of estimating fifty percent endpoints Phylogenetic characterization of Newcastle disease virus isolated in the mainland of China during Genotyping of infectious bronchitis viruses from broiler farms in Iraq during The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in Tombusviruses: implications for the mechanism of RNA recombination Co-infection with multiple respiratory pathogens contributes to increased mortality rates in Algerian poultry flocks Infectious bronchitis virus variants: a review of the history, current situation and control measures The experimental infection of chickens with mixtures of infectious bronchitis virus and Escherichia coli Recombination in avian gamma-coronavirus infectious bronchitis virus Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China Incorporation of spike and membrane glycoproteins into coronavirus virions Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus Evolutionary implications of genetic variations in the S1 gene of infectious bronchitis virus Some characteristics of isolates of infectious bronchitis virus from commercial vaccines Characterization and analysis of an infectious bronchitis virus strain isolated from southern China in 2013 Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination Molecular detection and smoothing spline clustering of the IBV strains detected in China during Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area Two novel neutralizing antigenic epitopes of the s1 subunit protein of a QX-like avian infectious bronchitis virus strain Sczy3 as revealed using a phage display peptide library This work was financially supported by the Program for Chang-jiang Scholars and Innovative Research Team in University PCSIRT (Grant No. IRT0848). Isolation, propagation, identification and comparative pathogenicity of five Egyptian field strains of Eimeria tenella from broiler chickens in five different provinces in Egypt Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum An in vitro system for the leader-primed transcription of coronavirus mRNAs Infectious bronchitis virus in different avian physiological systems-a field study in Brazilian poultry flocks Coronaviruses in poultry and other birds Phylogenetic analysis of hemagglutinin genes of 40 H9N2 subtype avian influenza viruses isolated from poultry in China from Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China Assessing the economic burden of avian infectious bronchitis on poultry farms in Brazil Serotyping of avian infectious bronchitis viruses by the virus-neutralization test Coronaviruses: an RNA proofreading machine regulates replication fidelity and diversity Progression of lesions in the respiratory tract of broilers after single infection with Escherichia coli compared to superinfection with E. coli after infection with infectious bronchitis virus Prevalence and molecular characterization of avian infectious bronchitis virus in poultry flocks in Morocco from 2010 to 2014 and first detection of Italy 02 in Africa Phylogenetic analysis of avian infectious bronchitis virus S1 glycoprotein regions reveals emergence of a new genotype in Moroccan broiler chicken flocks Isolation and identification of a Taiwan genotype I avian infectious bronchitis virus and the pathogenicity identification Genotypes of infectious bronchitis viruses circulating in the Middle East between Prevalence of avian respiratory viruses in broiler flocks in Egypt Epidemiology of avian infectious bronchitis virus genotypes in Iran Attenuation mechanism of virulent infectious bronchitis virus strain with QX genotype by continuous passage in chicken embryos Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus Survey indicates circulation of 4/91 and QX-type infectious bronchitis viruses in Hungary in 2014 -short communication Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Circulation of infectious bronchitis virus strains from Italy 02 and QX genotypes in Slovenia between RNA recombination in animal and plant viruses Cloning and identification of ipaJ gene in Salmonella pullorum Serotype and genotype diversity of infectious bronchitis viruses isolated during 1985-2008 in Guangxi Genetic diversity of avian infectious bronchitis coronavirus in recent years in China Molecular characterization of major structural protein genes of avian coronavirus
- keywords: 793b; analysis; antigenic; avian; breakpoint; bronchitis; bronchitis virus; cek; cells; characteristics; chicken; china; coronavirus; cqkx/150203; cross; different; epitopes; et al; field; flocks; gene; genotype; group; h120; ibvs; identity; immune; infectious; infectious bronchitis; isolates; m41; mass; molecular; neutralization; neutralizing; new; nucleotide; phylogenetic; poultry; previous; recent; recombinant; recombination; reference; relatedness; rna; samples; scdy/141030; scyb/140913; sczy3; sequences; sera; serotypes; site; southwestern; strains; study; subunit; table; twii; type; vaccine; variant-2; viral; virus; viruses; years; zou
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- cord-263785-0iift8zy
- author: Zhang, Xiaorong
- title: Evaluation of the reproductive system development and egg-laying performance of hens infected with TW I-type infectious bronchitis virus
- date: 2020-07-31
- words: 3703
- flesch: 44
- summary: In: Walker JM (ed) Methods in molecular biology Egg quality traits differ in hens selected for high as compared with low antibody response to sheep red blood cells Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks Characterization of the complete genome, antigenicity, pathogenicity, tissue tropism, and shedding of a recombinant avian infectious bronchitis virus with a ck/CH/LJL/140901-like backbone and an S2 fragment from a 4/91-like virus Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination Comparative histopathology and immunohistochemistry of QX-like, Massachusetts and 793/B serotypes of infectious bronchitis virus infection in chickens Pathogenicity differences between a newly emerged TW-like strain and a prevalent QX-like strain of infectious bronchitis virus Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine Genetic and pathologic characterization of a novel recombinant TC07-2-type avian infectious bronchitis virus Pathogenicity differences between QX-like and Mass-type infectious bronchitis viruses Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine A Multi-omics study of chicken infected by nephropathogenic infectious bronchitis virus Effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct Effects of the routine multiple vaccinations on the expression of innate immune molecules and induction of histone modification in ovarian cells of layer chicks Coronavirus avian infectious bronchitis virus Evolution of infectious bronchitis virus in China over the past two decades Recombination in avian gamma-coronavirus infectious bronchitis virus Induction of IBV strain-specific neutralizing antibodies and broad spectrum protection in layer pullets primed with IBV Massachusetts (Mass) and 793B vaccines prior to injection of inactivated vaccine containing Mass antigen The long view: 40 years of infectious bronchitis research A long-term study of the pathogenesis of infection of fowls with three strains of avian infectious bronchitis virus Re-excretion of infectious bronchitis virus in chickens induced by cyclosporin Phylogenetic analysis of the S1 glycoprotein gene of infectious bronchitis viruses isolated in China during Epidemiology and characterization of avian infectious bronchitis virus strains circulating in southern China during the period from Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China Altered pathogenicity of a tl/CH/LDT3/03 genotype infectious bronchitis coronavirus due to natural recombination in the 5′-17 kb region of the genome Genetics, antigenicity and virulence properties of three infectious bronchitis viruses isolated from a single tracheal sample in a chicken with respiratory problems Characterization and analysis of an infectious bronchitis virus strain isolated from southern China in 2013 Molecular epidemiology of infectious bronchitis virus in China between 2009 and 2011, and development of recombinant vaccine using Marek's disease virus as vector Development and efficacy of an attenuated TW-I like infectious bronchitis virus strain as a candidate live vaccine for chickens in China Coronaviruses: methods and protocols.
- keywords: age; avian; bronchitis; bronchitis virus; challenge; chickens; china; cloaca; collected; control; days; decrease; dpi; egg; eggs; female; figure; flock; group; hens; ibv; infected; infectious; infectious bronchitis; kidneys; laying; lesions; like; long; old; oviduct; pathogenicity; performance; period; production; quality; quantity; reproductive; respiratory; samples; shedding; spf; strains; study; symptoms; system; term; trachea; type; type ibv; virus; viruses
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- cord-264716-igl25jhg
- author: Koo, B.S.
- title: Molecular survey of enteric viruses in commercial chicken farms in Korea with a history of enteritis
- date: 2013-11-01
- words: 4986
- flesch: 38
- summary: A molecular survey was performed for a broad range of enteric viruses, namely CAstV, ANV, IBV, AvRV, ARV, and FAdV, in commercial chicken flocks suffering from enteritis. In this study, a molecular survey was performed for a broad range of enteric viruses including CAstV, ANV, ChPV, IBV, AvRV, ARV, and FAdV in intestine samples from commercial chicken flocks suffering from enteritis.
- keywords: amplification; analysis; anv; arv; astrovirus; avian; avrv; bacterial; broiler; bronchitis; castv; chicken; chicken flocks; chpv; commercial; conditions; contents; cycle; cycling; detection; enteric; enteric viruses; enteritis; et al; fadv; feed; figure; flocks; gene; group; high; ibv; infection; intestines; korea; lesions; min; molecular; partial; pcr; phylogenetic; polymerase; positive; reaction; report; reverse; rss; samples; sequence; spp; strains; stunting; syndrome; table; thermal; transcription; turkey; viruses; ° c
- versions: original; plain text
- cord-265258-2rmtsyns
- author: Domanska‐Blicharz, K.
- title: Specific detection of GII‐1 lineage of infectious bronchitis virus
- date: 2017-07-03
- words: 3414
- flesch: 46
- summary: The intra and interassay precision of the new method were assessed using serial dilutions of D1466 IBV RNA ranging from 8Á2 9 10 6 down to 5Á6 9 10 1 copies. The standard curve and linear regression analysis were performed using serial dilutions of known quantity of GII-1 IBV RNA (Fig. 1) .
- keywords: amplification; assay; bronchitis; chain; chicken; concentration; copies; d1466; detection; different; disease; et al; final; gene; gii‐1; ibv; identification; infectious; laboratories; like; lineage; method; min; molecular; nested; nmol; pcr; poland; positive; primers; probe; reaction; real; region; reverse; rna; samples; standard; strains; study; taqman; time; time rt; vaccine; values; variant; viral; virus
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- cord-265499-pbf11zy1
- author: Dove, Brian K.
- title: Changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus
- date: 2006-03-03
- words: 5030
- flesch: 36
- summary: The ability of N protein to localize to the nucleolus was confirmed using live cell imaging of cells expressing EGFP fused to the N-terminus of IBV N protein (EGFP-IBV N protein) ( Fig. 4C ) IBV N protein (Beaudette strain) is a 409-amino-acid basic protein with a predicted molecular weight of 45 kDa.
- keywords: analysis; andersen; antibody; apparent; b23; bronchitis; cells; changes; chen; chen et; confocal; content; coronavirus; cycle; cytoplasm; data; dfc; distribution; dsred; egfp; et al; fig; fluorescent; functions; growth; host; ibv; ibv n; imaging; infected; infected cells; infection; interaction; invitrogen; kda; localization; marker; membrane; microscopy; mock; molecular; morphology; n protein; nuclear; nucleocapsid; nucleolar; nucleolin; nucleolus; nucleus; p53; polyclonal; protein; purified; red; replication; rna; rowland; signal; transcription; tumour; vero; virus; viruses
- versions: original; plain text
- cord-265508-t1nfyzf5
- author: Boursnell, M.E.G.
- title: Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B
- date: 1984-08-31
- words: 2134
- flesch: 51
- summary: RNA sequence data from this region might also enable us to predict the properties and thus aid the identification of possible polypeptides coded for by mRNA B. key: cord-265508-t1nfyzf5 authors: Boursnell, M.E.G.; Brown, T.D.K. title: Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B date: 1984-08-31 journal: Gene DOI: 10.1016/0378-1119(84)90169-0 sha: doc_id: 265508 cord_uid: t1nfyzf5 Abstract DNA sequencing of genomic cDNA clones of avian infectious bronchitis virus (IBV) has been carried out.
- keywords: avian; boursnell; brown; cdna; coding; coronavirus; dna; end; et al; genome; genomic; ibv; infectious; initiation; messenger; mhv; mrna; nucleocapsid; orf; polypeptides; present; protein; rna; rnas; sequence; siddell; smallest; stern; structural; translation; viral
- versions: original; plain text
- cord-265681-ab8j4o1u
- author: Boroomand, Zahra
- title: Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens
- date: 2012-04-01
- words: 3556
- flesch: 49
- summary: The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus Histopathology and immunohistochemistry of renal lesions due to infectious bronchitis virus in chicks Comparative study of respiratory lesions in two chicken lines of different susceptibility infected with infectious bronchitis virus: histology, ultrastructure and immunohistochemistry Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos LNA probe-based real-time RT-PCR for the detection of infectious bronchitis virus from the oviduct of unvaccinated and vaccinated laying hens Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions Isolation and identification of a new isolate of avian infectious bronchitis virus IRFIBV32 and study of its pathogenicity A simple method of estimating fifty per cent endpoints Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction Serological and gross findings in broilers with AIV and IBV The histopathology of infectious bronchitis in the domestic fowl Pathogenicity of infectious bronchitis virus isolates from Ontario chickens Comparison of the nephropathogenicity of four strains of infectious bronchitis virus Pathogenicity of Australian strains of avian infectious bronchitis virus Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes Isolation, identification and molecular characterization of IBV variant from out break of visceral gout in commercial broilers Experimental histopathologic study of the lesions induced by serotype 793/B (4/91) infectious bronchitis virus Detection the 4/91 strain of infectious bronchitis virus in testicular tissue from experimentally infected rooster by reverse transcription-polymerase chain reaction Histopathology and immunohistochemistry of renal lesions due to avian infectious bronchitis virus in chicks uninoculated and previously inoculated with highly virulent infectious bursal disease virus The histopathology of infectious bronchitis in fowls infected with a nephrotropic T strain of virus Tissue tropism of three cloacal isolates and Massachusetts strain of infectious bronchitis virus The pathogenesis of nephritis in chickens induced by infectious bronchitis virus Tissue distribution of avian infectious bronchitis virus following in ovo inoculation of chicken embryos examined by in situ hybridization with antisense digoxigenin-labeled universal riboprobe Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody Prevalence of antibodies to infectious bronchitis virus (IBV) in chickens in southwestern Nigeria
- keywords: antibodies; antibody; avian; birds; bronchitis; bronchitis virus; caecal; chickens; chicks; clinical; control; days; detection; digestive; disease; distribution; elisa; experimental; gross; group; ibv; immunohistochemistry; infected; infectious; infectious bronchitis; inoculated; inoculation; irfibv32; isolate; isolation; kidney; lesions; oviduct; pathogenicity; pcr; respiratory; rna; samples; signs; strain; study; tissues; tonsil; trachea; viral; virus
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- cord-266585-jfjrk9gy
- author: Fang, Shouguo
- title: An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells
- date: 2007-02-05
- words: 7179
- flesch: 49
- summary: In vitro assembly of full-length coronavirus clones, generation of full-length transcripts in vitro using a bacteriophage DNA-dependent RNA polymerase, and recovery of infectious viruses by introduction of the in vitro-synthesized transcripts into cells, first used by Yount et al. (2000) , are a rapid and reliable approach to construct infectious clones from large RNA viruses. key: cord-266585-jfjrk9gy authors: Fang, Shouguo; Chen, Bo; Tay, Felicia P.L.; Ng, Beng Sern; Liu, Ding Xing title: An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells date: 2007-02-05 journal: Virology DOI: 10.1016/j.virol.2006.08.020 sha: doc_id: 266585 cord_uid: jfjrk9gy Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses.
- keywords: acid; amino; analysis; anti; bronchitis; cdna; cells; characterization; cleavage; clone; coronavirus; days; domain; electroporation; et al; fig; fragments; g15526c; gel; gene; genome; growth; helicase; ibv; infected; infectious; infectivity; lane; length; length transcripts; like; mrna; mutant; mutation; negative; pcr; position; post; products; properties; protein; r132k; recombinant; region; replication; residue; reverse; ribv; rna; rnas; sequencing; similar; subgenomic; total; transcription; transcripts; transfected; type; vero; vero cells; viral; virus; viruses; vitro; wild
- versions: original; plain text
- cord-268454-w4qca90s
- author: Rim, Aouini
- title: Viral interference between H9N2-low pathogenic avian influenza virus and avian infectious bronchitis virus vaccine strain H120 in vivo
- date: 2019-06-17
- words: 4710
- flesch: 41
- summary: A Expression level in chickens Role of infectious bronchitis live vaccine on pathogenicity of H9N2 avian influenza virus Experimental co-infection of infectious bronchitis and low pathogenic avian influenza H9N2 viruses in commercial broiler chickens Avian respiratory co infection and impact onavian influenza pathogenicity in domestic poultry: field and experimental findings Selective isolation of avian influenza virus (AIV) from cloacal samples containing AIV and Newcastle disease virus Novel gene mutations in tunisian isolate of avian H9N2 influenza virus A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys Experimental co-infections of domestic ducks with a virulent Newcastle disease virus and low or highly pathogenic avian influenza viruses Clinico-pathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks The pathogenesis of low pathogenicity H7 avian influenza viruses in chickens, ducks and turkeys A multiplex real-time RT-PCR for simultaneous detection of four most common avian respiratory viruses An experimental study on broiler chicken coinfected with the specimens containing avian influenza (H9 subtype) and infectious bronchitis (4/91 strain) viruses The high susceptibility of turkeys to influenza viruses of different origins implies their importance as potential intermediate hosts Co-cultivation of avian orthomyxoviruses and paramyxoviruses in embryonated eggs: implications for surveillance studies An overview of influenza A virus receptors Inoculation time differentials for expression of interference of Newcastle disease virus by swine influenza virus in chick embryos Homologous interference induced by a temperature-sensitive mutant derived from an HVJ (Sendaivirus) carrier culture Binding of avian coronavirus spike proteins to host factors reflects virus tropism and pathogenicity Interference of inactive virus with the propagation of virus of influenza Interference between the Influenza viruses: Super-infected groups showed that IBV virus is detected in TC and CL swabs from 3 to 20dpi in chicks infected with AIV followed by IBV inoculated 3 days later; no AIV shedding being noticed.
- keywords: 20dpi; aiv; antibody; avian; birds; broiler; bronchitis; cell; chickens; chicks; clinical; collected; control; days; disease; elisa; experimental; factors; figs; groups; growth; h120; h9n2; histopathological; ibv; induced; infected; infection; influenza; inhibition; inoculated; interference; interferon; lesions; levels; low; lower; mixture; newcastle; noticed; organs; pathogenicity; pcr; poultry; qrt; replication; respiratory; results; shedding; significant; signs; single; strain; studies; study; super; swabs; test; titers; vaccination; vaccine; viral; virus; viruses
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- cord-268788-jcu3pasy
- author: Thor, Sharmi W.
- title: Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus
- date: 2011-09-23
- words: 4430
- flesch: 43
- summary: In addition, the only fragments that USA viruses have in common with the viruses from China and Taiwan are fragments also associated with Mass type vaccines, which are used in both regions, providing further evidence that some of the fragments in field viruses come from vaccines. That result and the observation in Figure 1 that the viruses separated into clades based on geographic location also supports the conclusion that USA viruses have not recombined with Asian viruses.
- keywords: addition; analysis; area; avian; breakpoints; bronchitis; conn; coronavirus; data; different; emergence; entire; evidence; evolution; field; fragments; gamma; genbank; genome; h120; high; ibv; identical; infectious; isolates; kit; length; manufacturer; mass; min; new; nsp; number; pcr; phylogenetic; proteins; reaction; recombination; regions; replication; rna; role; sequence; serotypes; size; spike; step; strains; study; table; trees; type; usa; vaccine; viral; virus; viruses; window; ° c
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- cord-269024-re0hyolh
- author: Bande, Faruku
- title: Prediction and In Silico Identification of Novel B-Cells and T-Cells Epitopes in the S1-Spike Glycoprotein of M41 and CR88 (793/B) Infectious Bronchitis Virus Serotypes for Application in Peptide Vaccines
- date: 2016-09-07
- words: 2570
- flesch: 41
- summary: Similarly, epitopes peptides found within RBD have been reported in different study to induce neutralizing antibody response, for example, against human coronavirus and mouse hepatitis virus One of the innovations in using this technology is the ability to incorporate several epitope peptides directed against different viruses and/or multiple virus strains into one single delivery system with the view to induce broad and specific immune response in single administration [11] .
- keywords: amino; analysis; antigenic; antigenicity; associated; avian; binding; bronchitis; cells; challenges; chicken; class; conservancy; conserved; control; coronavirus; cr88; ctl; different; dna; epitopes; glycoprotein; ibv; immune; index; infectious; linear; live; m41; mhci; novel; peptides; poultry; prediction; response; strains; study; use; vaccines; virus
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- cord-269150-d1sgnxc0
- author: Tan, Yong Wah
- title: Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription
- date: 2012-02-22
- words: 6997
- flesch: 41
- summary: Western blot analysis also noted a reduction in the expression of viral structural genes, between 50% and 90% reduction for S and N proteins, with a reduction between 40% and 80% of MADP1 protein ( Figure 5B ). key: cord-269150-d1sgnxc0 authors: Tan, Yong Wah; Hong, Wanjin; Liu, Ding Xiang title: Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription date: 2012-02-22 journal: Nucleic Acids Res DOI: 10.1093/nar/gks165 sha: doc_id: 269150 cord_uid: d1sgnxc0 Coronaviruses RNA synthesis occurs in the cytoplasm and is regulated by host cell proteins.
- keywords: -utr; acid; activity; amino; anti; assay; binding; biotin; bronchitis; cells; control; coronavirus; cov; cytoplasm; domain; efficiency; egfp; expression; figure; flag; functional; genome; h1299; hcov; host; human; ibv; infected; infection; infectivity; interaction; length; localization; loop; luciferase; lysates; madp1; motif; mrna; mutant; negative; non; nuclear; nucleocapsid; presence; protein; pull; reduction; region; replication; residues; rna; rna synthesis; rnas; role; rrm; rtcs; sars; screen; secondary; sense; sequence; shmadp1; silencing; sirna; specific; stem; structure; studies; study; subgenomic; synthesis; transcription; triple; type; unlabeled; viral; viral rna; wild
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- cord-271359-dpa8zzc3
- author: Sapats, S. I.
- title: Novel Variation in the N Protein of Avian Infectious Bronchitis Virus
- date: 1996-12-15
- words: 2320
- flesch: 61
- summary: These epi-1962, whereas N9/74, N2/75, N1/88, Q3/88, V5/90, and V18/91 were isolated from vaccinated commercial chicks topes are conserved in other Australian strains of IBV, indicating unusual changes in the N genes of N1/88, Q3/ between 1974 and 1991. 3b ) showed other strains of IBV based upon the published nucleotide sequences for the N gene is shown in Fig. 2 .
- keywords: acid; amino; australian; basic; conservation; conserved; downstream; fig; gene; group; hvr; ibv; identity; n1/62; n1/88; n2/75; positions; protein; q3/88; region; residues; rna; sequences; serine; strains; utr; v18/91; vic; virus
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- cord-271568-qgpi2kcs
- author: Jackwood, M.W.
- title: Avian coronavirus infectious bronchitis virus susceptibility to botanical oleoresins and essential oils in vitro and in vivo
- date: 2010-01-21
- words: 7617
- flesch: 52
- summary: Tracheas were scored as described in Section 2 with 1 being normal and 4 being severe, and the birds that did not receive challenge virus had an average lesion score of 1.06. A less pronounced response was observed in birds treated 2 h after virus challenge.
- keywords: activity; antibodies; arkansas; average; birds; bronchitis; cells; challenge; chickens; clinical; clinical signs; commercial; contact; control; coronavirus; culture; data; days; different; diluent; dilution; disease; dose; effect; effective; eggs; essential; experiment; eyes; groups; ibv; inc; infectious; mass41; mucus; nares; negative; non; normal; oils; pathogenic; pcr; positive; post; qr448(a; real; rna; scores; severe; signs; spray; strain; table; test; time; titer; trachea; transmission; treated; treatment; type; undiluted; vaccine; vero; viral; virucidal; virus; viruses; watery
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- cord-271897-9oqzsd70
- author: Domanska-Blicharz, Katarzyna
- title: Molecular epidemiology of infectious bronchitis virus in Poland from 1980 to 2017
- date: 2020-01-07
- words: 6560
- flesch: 46
- summary: The recombination analysis showed that Polish strains are a mosaic of different parental viruses most likely resulting from recombination events involving different IBV lineages, most frequently GI-13 and GI-19. Irrespective of year of isolation and S1-dependent genotype, the genome sequences of Polish IBV strains showed the presence of six genes and 13 ORFs: 5′UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-3′UTR, however their individual genes and putative proteins had different lengths.
- keywords: 1980s; 2012; 79/1989; analysis; avian; blicharz; bootstrap; branch; broiler; bronchitis; bronchitis virus; characterization; chickens; china; cluster; complete; coronavirus; department; different; domanska; early; end; et al; europe; events; field; fig; flocks; genes; genome; genotype; gi-19; gi-23; ibv; ibv lineages; ibv strains; ibvs; identity; infectious; infectious bronchitis; introduction; isolates; kit; laboratory; like; lineage; mass; molecular; new; nucleotide; ones; orfs; period; phylogenetic; poland; polish; polish ibv; polish strains; poultry; presence; proteins; recombination; region; respiratory; rna; separate; sequences; sequencing; similarity; strains; study; subsequent; time; tree; unique; vaccine; variants; virus; viruses; years
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- cord-272305-eniovfwy
- author: Zhao, Ye
- title: Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine
- date: 2015-10-22
- words: 5932
- flesch: 49
- summary: a YN group infected with YN strain; aYN group infected with attenuated YN strain. virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virusneutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo Infectious bronchitis Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus The long view: 40 years of infectious bronchitis research An assessment of opportunities to dissect host genetic variation in resistance to infectious diseases in livestock Virulent avian infectious bronchitis virus, People's Republic of China Efficacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens Effect of serial embryo passage of an Arkansas-type avian infectious bronchitis virus isolate on clinical response, virus recovery, and immunity Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains Attenuation, safety, and efficacy of an infectious bronchitis virus GA98 serotype vaccine Data from 11 years of molecular typing infectious bronchitis virus field isolates Infectious bronchitis virus: S1 gene characteristics of vaccines used in China and efficacy of vaccination against heterologous strains from China S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryopassaged, attenuated derivatives Complete genome sequence analysis of a predominant infectious bronchitis virus (IBV) strain in China Infectious bronchitis virus: immunopathogenesis of infection in the chicken A simple method of estimating fifty per cent endpoints Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes Intracloacal infection with avian infectious bronchitis virus QXtype infectious bronchitis virus in commercial flocks in the UK Isolation and identification of glandular stomach type IBV (QX IBV) in chickens Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene Molecular characterization of an infectious bronchitis virus strain isolated from northern China in 2012 Comparison of the pathogenicity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions This study was supported by Beijing Agriculture Innovation Consortium of Poultry Research System.
- keywords: attenuated; avian; ayn; birds; bronchitis; bronchitis virus; bursa; challenge; chickens; chicks; china; ciliostasis; clinical; commercial; control; damage; day; different; dpi; duodenum; efficacy; eggs; eid; epithelium; et al; field; fig; gene; gross; group; heterologous; homologous; ibv; infected; infectious; infectious bronchitis; inoculated; kidney; lesions; like; mean; movement; number; old; organs; pathogenicity; pcr; poultry; protection; proventriculus; renal; respiratory; samples; score; sections; serotypes; severe; significant; signs; spf; strain; time; tissue; trachea; tract; type; vaccinated; vaccine; viral; virulent; virus; yn strain
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- cord-272437-gvzfl8c3
- author: Zhao, Jing
- title: Replicase 1a gene plays a critical role in pathogenesis of avian coronavirus infectious bronchitis virus
- date: 2020-08-20
- words: 566
- flesch: 39
- summary: The results revealed that mutations in the ORF1a gene during passage in embryonated eggs caused the decreased pathogenicity of virulent IBV YN strain, proven by determination of virus replication in ECEs and CEK cells, the observation of clinical signs, gross lesions, microscopic lesions, tracheal ciliary activity and virus distribution in chickens following exposure to rIBVs. is critical for viral virulence Infectious bronchitis virus variants: a 425 review of the history, current situation and control measures Chapter three -The nonstructural proteins directing 427 coronavirus RNA synthesis and processing Recombinant live attenuated avian coronavirus 431 vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious 432 bronchitis in chickens Coronavirus pathogenesis Attenuation, safety, and efficacy of a QX-like infectious bronchitis virus serotype 445 vaccine Porcine deltacoronavirus: Overview of infection dynamics, diagnostic methods, 447 prevalence and genetic evolution attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine Successful establishment of a reverse 452 genetic system for QX-type infectious bronchitis virus and technical improvement of the 453 rescue procedure S gene and 5a accessory gene 455 are responsible for the attenuation of virulent infectious bronchitis coronavirus Coronavirus replicative proteins Virus-encoded proteinases and proteolytic 460 processing in the Nidovirales
- keywords: ayn; bronchitis; coronavirus; fig; gene; group; ibv; infectious; pathogenicity; ryn-1a; strain; virus
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- cord-272666-3uidpr79
- author: Doyle, Nicole
- title: Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing
- date: 2018-09-06
- words: 7506
- flesch: 48
- summary: As the role in membrane rearrangements for nsp3 and 4 is well-established for several nidoviruses and considering that nsp6 may also play some role, here we investigated the role that these three nsps play in the formation of IBV membrane rearrangements. A Key Role for Nonstructural Protein 3 in the Remodeling of Intracellular Membranes Open Reading Frame 1a-Encoded Subunits of the Arterivirus Replicase Induce Endoplasmic Reticulum-Derived Double-Membrane Vesicles Which Carry the Viral Replication Complex Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex Evolving the largest RNA virus genome Membrane rearrangements mediated by coronavirus nonstructural proteins 3 and 4.
- keywords: 3xflag; able; addition; anti; areas; associated; beaur; bronchitis; cells; cellular; complex; coronavirus; cov; cytoplasmic; df1; df1 cells; different; disordered; dmvs; double; electron; endoplasmic; expression; figure; fisher; formation; gfp; hepatitis; host; ibv; induced; infected; infectious; interaction; large; length; like; localization; m41; mcherry; membrane; membrane rearrangements; mers; mhv; nonstructural; nsp3; nsp4; nsp6; nsps; pairing; pbs; plasmids; presence; proteins; puncta; rearrangements; replication; result; reticulum; rna; role; sars; scientific; sections; small; spherules; strains; structures; study; vesicles; viral; virus; viruses; zer
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- cord-272693-432ixb7g
- author: Phillips, J. E.
- title: Changes in nonstructural protein 3 are associated with attenuation in avian coronavirus infectious bronchitis virus
- date: 2011-09-10
- words: 6285
- flesch: 52
- summary: The percent amino acid identity for the S glycoprotein is 97.8% for Ark viruses, 96.6% for GA98 viruses, and 97.2% for Mass 41 viruses (Fig. 3) . To examine the sequence changes in individual genes associated with attenuation of IBV for chickens, we sequenced and compared the full-length consensus genomes of pathogenic IBV viruses and egg-passaged attenuated (for chickens) viruses from three different serotypes.
- keywords: 1ab; acid; addition; adp; amino; amino acid; amplification; analysis; ark; ark viruses; attenuated; avian; birds; bronchitis; changes; chickens; coronaviruses; cwl0470/98; data; differences; different; domain; dpi/81; eggs; ga98; ga98 viruses; genes; genome; glycoprotein; ibv; identity; infectious; length; mass; mass41/41; membrane; nsp; nsps; number; orf; pathogenic; pathogenic virus; pathogenicity; pcr; pl2; primer; protease; protein; reaction; replication; rna; role; sequence; sequencing; serotype; spike; strain; table; time; transcription; transmembrane; viral; virus; viruses
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- cord-273366-xd84f8ct
- author: Brownsword, Matthew J.
- title: Infectious Bronchitis Virus Regulates Cellular Stress Granule Signaling
- date: 2020-05-14
- words: 8918
- flesch: 43
- summary: When IBV infected cells were treated with sodium arsenite, there was also a significant increase in the level of phosphorylated eIF2α when compared to IBV infected untreated cells. When IBV infected cells were treated with sodium arsenite, there was also a significant increase in the level of phosphorylated eIF2α when compared to IBV infected untreated cells.
- keywords: activation; addition; anti; antibody; antiviral; arsenite; assembly; canonical; cells; cellular; control; coronavirus; cycloheximide; dsrna; eif2α; eif4; figure; foci; formation; g3bp1; genomic; granules; host; hpi; hydrogen; ibv; ibv infected; ibv infection; independent; induction; infected; infected cells; infection; inhibition; inhibits; initiation; kinase; levels; markers; mock; mrna; multiple; pathways; percentage; peroxide; phosphorylation; pkr; points; protein; puncta; puromycin; replicates; replication; response; rna; rnase; shut; signaling; sites; sodium; specific; stress; time; translation; treatment; vero; vero cells; viral; virus; viruses
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- cord-273661-egpyvqrw
- author: Mo, Mei-Lan
- title: Molecular Characterization of Major Structural Protein Genes of Avian Coronavirus Infectious Bronchitis Virus Isolates in Southern China
- date: 2013-12-04
- words: 4729
- flesch: 47
- summary: Correlation with biological functions Immune responses to structural proteins of avian infectious bronchitis virus Rapid detection and identification of avian infectious bronchitis virus Typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the S1 gene The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction Molecular epidemiology and evolution of avian infectious bronchitis virus in Spain over a fourteen-year period Attenuated live vaccine usage affects accurate measures of virus diversity and mutation rates in avian coronavirus infectious bronchitis virus Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DP Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06 Serotype and genotype diversity of infectious bronchitis viruses isolated during 1985-2008 in Guangxi Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Complete genome sequences of two chinese virulent avian coronavirus infectious bronchitis virus variants Genetic diversity of spike, 3a, 3b and e genes of infectious bronchitis viruses and emergence of new recombinants in Korea Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3 Genetic diversity of avian infectious bronchitis coronavirus in recent years in China Molecular epidemiological investigation of Marek's disease virus from Guangxi Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus Genetic diversity of avian infectious bronchitis virus California variants isolated between 1988 and 2001 based on the S1 subunit of the spike glycoprotein Recombinational histories of avian infectious bronchitis virus and turkey coronavirus Complete nucleotide sequences of S1 and N genes of infectious bronchitis virus isolated in Japan and Taiwan Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan Differential stepwise evolution of SARS coronavirus functional proteins in different host species Identification of intertypic recombinant infectious bronchitis viruses from slaughtered chickens Evolution of infectious bronchitis virus in Taiwan: Positively selected sites in the nucleocapsid protein and their effects on RNA-binding activity Quantifying selection and diversity in viruses by entropy methods, with application to the haemagglutinin of H3N2 influenza Incorporating background frequency improves entropy-based residue conservation measures Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus A Shannon entropy analysis of immunoglobulin and Tcell receptor Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: Identification of intertypic recombination in the S1 gene Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120 Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3' untranslated region Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during This work was supported by grants from the National Natural Science Foundation of China (31360611, 31160516), Guangxi Natural Science Foundation (2013GXNSFCA019010), and the Guangxi Provincial Programs for Science and Technology Development (0993009-2).
- keywords: acid; amino; amino acid; analysis; avian; bronchitis; china; coronavirus; different; diversity; emergence; entropy; evolution; field; gene; genetic; genotypes; high; ibv; ibvs; important; infectious; infectious bronchitis; isolates; lsc/99i; lx4; molecular; n gene; new; nucleotide; phylogenetic; positive; protein; recombinants; recombination; region; results; s1 gene; selection; sequences; sites; spike; strains; study; taiwan; trees; type; vaccine; variants; variation; virus; viruses
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- cord-273846-l0elcfe8
- author: Ganapathy, Kannan
- title: Effects of cold storage on detection of avian infectious bronchitis virus in chicken carcasses and local antibodies in tracheal washes
- date: 2005-02-24
- words: 2442
- flesch: 51
- summary: key: cord-273846-l0elcfe8 authors: Ganapathy, Kannan; Cargill, Peter Walker; Jones, Richard Charles title: Effects of cold storage on detection of avian infectious bronchitis virus in chicken carcasses and local antibodies in tracheal washes date: 2005-02-24 journal: J Virol Methods DOI: 10.1016/j.jviromet.2005.01.024 sha: doc_id: 273846 cord_uid: l0elcfe8 In order to test the survivability of infectious bronchitis virus (IBV) in dead chicken carcasses during 24 h of cold storage, 7 week-old specific-pathogen-free chickens were infected with virulent IBV Massachusetts strain M41, and were killed humanely 10 days later. Early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus Co-circulation of four types of infectious bronchitis virus (793/B 624/1, B1648 and Massachusetts) Infectious bronchitis Longitudinal field studies on infectious bronchitis and avian pneumovirus in broiler using type-specific PCR Coronaviridae The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus Comparative growth kinetic studies on avian infectious bronchitis virus in different systems Detection of infectious bronchitis Local antibody production in the oviduct and gut of hens infected with a variant strain of infectious bronchitis virus Infectious bronchitis virus: immunopathogenesis of infection in chicken Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptasepolymerase chain reaction Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction Inactivated rabies vaccine control and release: use of an ELISA method A Laboratory Manual for the Isolation and Identification of Avian Pathogens Local antibody response in avian infectious bronchitis: virus-neutralizing antibody in tracheobronchial secretions Local immunity to avian infectious bronchitis in tracheal organ culture Presence of viral antigens and antibody in the trachea of chickens infected with avian infectious bronchitis virus Further development and use of a molecular serotype identification test for infectious bronchitis virus Polymerase chain reaction a biotin-labelled DNA probe for detection of infectious bronchitis virus in chickens Tissue tropism of three cloacal isolates and Massachusetts strain of infectious bronchitis virus
- keywords: avian; bronchitis; carcasses; cavanagh; chicken; days; detection; dhinakar; ece; ibv; iga; infectious; isolation; jones; kidney; levels; local; naqi; pcr; post; raj; rectum; sampling; specific; storage; swabs; tissues; tracheal; virus; washes
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- cord-276550-1in7m56w
- author: Abdel-Moneim, Ahmed S
- title: S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
- date: 2006-09-20
- words: 3969
- flesch: 42
- summary: Thirty chickens were infected by intraocular instillation of 10 5 EID 50 /100 μl of Infectious bronchitis Virus Taxonomy Coronaviruses in poultry and other birds Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Identification of amino acids involved in a serotype and neutralization specific epitope within the S1 subunit of avian infectious bronchitis virus Analysis of the serotypespecific epitopes of avian infectious bronchitis virus strains Ark99 and Mass41 Epitopes on the peplomer protein of infectious bronchitis coronavirus strain M41 as defined by monoclonal antibodies A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism Differentiation of infectious bronchitis virus serotypes using the polymerase chain reaction and restriction fragment length polymorphism analysis Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo Location of the amino-acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide Present status of infectious bronchitis in Egypt Studies on the epidemiology and means of control ofinfectious bronchitis disease in chickens in Egypt Isolation and identification of Egypt/Beni-Suef/01 a novel genotype of infectious bronchitis virus Urolithiathis in white commercial egg laying chickens associated with an infectious bronchitis virus Differences inpathogenicity for young chickens among field isolates of infectious bronchitis virus Studies on the prevalence and epidemiology of infectious bronchitis in broiler chickens in El-Minia Governorate The histopathology of infectious bronchitis in fowls infected with a nephrotropic T strain of virus Comparison of the nephropathogenicity of four strains of infectious bronchitis virus The control of avian infectious bronchitis/nephrosis in Australia In vitro characterization and pathogenesis of Egypt/Beni-Suef/01; a novel genotype of infectious bronchitis virus Criteria for examining the immune response to infectious bronchitis virus Some characteristics of isolates of infectious bronchitis virus from commercial vaccines The immune response to infectious bronchitis virus determined by respiratory signs, virus infection, and histopathological lesions Challenge experiments to evaluate cross-protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus Protection of chickens following live and inactivated virus vaccination against challenge with nephropathogenic infectious bronchitis virus PA/Wolgemuth/98 Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Vaccination of day-old broilers against infectious bronchitis: Effect of vaccine strain and route of administration Vaccination of one day-old broilersagainst infectious bronchitis by eye drop application or coarse droplet spray and the effect of revaccination by spray Lack of correlation between infectivity, serological response, and challenge results in immunization with an avian infectious bronchitis vaccine Comparison of the spike precursor sequences of coronavirus IBV strains M41 and 6/82with that of IBV Beaudette Phylogeny of antigenic variants of avian coronavirus IBV. Abbreviations CAM, chorioallantoic membrane; ECE, Embryonated chicken eggs; EID, egg infective dose fifty; FITC, fluorescien isothiocynate; HVR, hypervariable region; IBV, infectious bronchitis virus; NCM, nitrocellulose membrane; PBS, phosphate buffer saline; RT, reverse transcriptase; SPF, specific-pathogen-free.
- keywords: acid; amino; analysis; antibodies; avian; birds; broiler; bronchitis; challenge; changes; chickens; control; coronavirus; day; days; dead; different; disease; dot; egypt; elisa; epitopes; f/03; fig; gene; glycoprotein; group; h120; ibv; identities; identity; infectious; infiltration; isolates; isolation; kidney; lesions; m41; nucleotide; old; pcr; protection; renal; respiratory; sequence; serotype; severe; spike; strains; study; tracheal; vaccinated; vaccination; vaccine; virus
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- cord-276755-ctzrgqe7
- author: Emmott, Edward
- title: Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus
- date: 2010-09-08
- words: 2501
- flesch: 28
- summary: This had not been previously observed in the nucleolus of IBVinfected DF1 cells, possibly because of the difficulties associated with the indirect immunofluorescence detection of nucleolar proteins [25] . The data indicated that, in general, there was an increase in nucleolar proteins in IBV-infected cells that was validated by indirect immunofluorescence confocal microscopy.
- keywords: analysis; avian; bronchitis; cells; cellular; changes; coronavirus; cycle; data; database; fig; fold; human; ibv; increase; infected; infectious; information; localization; mock; nuclear; nucleolar; nucleolar proteome; nucleolus; potential; proteins; proteome; proteomics; quantitative; replication; rna; silac; study; viral; virus; viruses
- versions: original; plain text
- cord-277804-ujabzic4
- author: Yuk, Seong-su
- title: Comparison between dot-immunoblotting assay and clinical sign determination method for quantifying avian infectious bronchitis virus vaccine by titration in embryonated eggs
- date: 2016-01-21
- words: 4161
- flesch: 46
- summary: United States Department of Agriculture Animal and Plant Health Inspection Service Protection induced by infectious laryngotracheitis virus vaccines alone and combined with Newcastle disease virus and/or infectious bronchitis virus vaccines Understanding interobserver agreement: the kappa statistic Respiratory signs, immunity response, and interference from vaccination with monovalent and multivalent infectious bronchitis vaccines This work was supported by a grant from the Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (no. 314017-03). key: cord-277804-ujabzic4 authors: Yuk, Seong-su; Kwon, Jung-Hoon; Noh, Jin-Yong; Hong, Woo-tack; Gwon, Gyeong-Bin; Jeong, Jei-Hyun; Jeong, Sol; Youn, Ha-Na; Heo, Yong-Hwan; Lee, Joong-Bok; Park, Seung-Yong; Choi, In-Soo; Song, Chang-Seon title: Comparison between dot-immunoblotting assay and clinical sign determination method for quantifying avian infectious bronchitis virus vaccine by titration in embryonated eggs date: 2016-01-21 journal: J Virol Methods DOI: 10.1016/j.jviromet.2016.01.008 sha: doc_id: 277804 cord_uid: ujabzic4 A sensitive and specific method for measuring the vaccine titer of infectious bronchitis virus (IBV) is important to commercial manufacturers for improving vaccine quality.
- keywords: /ml; assay; attenuated; bronchitis; chicken; clinical; days; detection; determined; dia; diluted; dilution; disease; ece; ee index; eggs; eid; embryos; higher; ibv; index; infectious; inoculated; inoculation; k40/09; k40/09hp40; km91; live; method; mock; negative; pcr; positive; propagated; ratio; real; results; samples; signs; strains; time; time rt; titer; titration; vaccine; value; virus
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- cord-278324-eqqvwwh6
- author: Wang, Huanan
- title: Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis viruses
- date: 2018-09-21
- words: 3515
- flesch: 42
- summary: The simultaneous quantification of ILTV and IBV antibodies were achieved through the interrogation of microspheres by Luminex 200 detection system. . Relevant positive and negative sera have been prepared for the establishment of the singleplex xMAP assay to detect ILTV antibody or IBV antibody in the serum.
- keywords: antibodies; antibody; assay; avian; beads; bronchitis; chicken; clinical; detection; diagnosis; differential; dilution; diseases; duplex; elisa; evaluation; glycoprotein; high; ibv; iltv; industry; infectious; laryngotracheitis; luminex; magbeads; method; microspheres; multiplex; ndv; optimal; positive; poultry; protein; recombinant; respiratory; results; samples; sensitivity; sera; serum; simultaneous; singleplex; study; table; technology; value; virus; xmap; xmap assay
- versions: original; plain text
- cord-279495-zxerb7de
- author: Liu, Xiaoli
- title: Comparative analysis of four Massachusetts type infectious bronchitis coronavirus genomes reveals a novel Massachusetts type strain and evidence of natural recombination in the genome
- date: 2012-11-21
- words: 5252
- flesch: 42
- summary: A filterable virus, distinct from that of laryngotracheitis, the cause of a respiratory disease of chicks Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: a review An efficient ribosomal frame-shifting signal in the polymeraseencoding region of the coronavirus IBV Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Commentary: a nomenclature for avian coronavirus isolates and the question of species status Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Coronaviruses in poultry and other birds Coronavirus avian infectious bronchitis virus Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Infectious bronchitis Variations in the spike protein of the 793/B type of infectious bronchitis virus in the field and during alternate passage in chickens and embryonated eggs A cis-acting function for the coronavirus leader in defective interfering RNA replication Virus Taxonomy, Classification and Nomenclature of Viruses, Ninth Report of the International Committee on Taxonomy of Viruses, International Union of Microbiological Societies, Virology Division Molecular epidemiology and evolution of avian infectious bronchitis virus in Spain over a fourteen-year period The early history of infectious bronchitis Evidence for variable rates of recombination in the MHV genome Map locations of mouse hepatitis virus temperature sensitive mutants: confirmation of variable rates of recombination A hypervariable region within the 3 0 cis-acting element of the murine coronavirus genome is nonessential for RNA synthesis but affects pathogenesis A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Biologic, antigenic, and full-length genomic characterization of a bovine-like coronavirus isolated from a giraffe Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus The present of viral subpopulations in an infectious bronchitis virus vaccine with differing pathogenicity -a preliminary study Recombinational histories of avian infectious bronchitis virus and turkey coronavirus Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase A novel variant of infectious bronchitis virus resulting from recombination among three different strains The neutralizing characteristics of strains of infectious bronchitis virus as measured by the constant virus variable serum methods in chicken tracheal cultures Experimental evidence of recombination in coronavirus infectious bronchitis virus Molecular analysis of infectious bronchitis viruses isolated in Slovenia between 1990 and 2005: a retrospective study Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus Origin and evolution of Georgia 98 (GA98), a new serotype of avian infectious bronchitis virus The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase RNA recombination in a coronavirus: recombination between viral genomic RNA and transfected RNA fragments Molecular characterization and pathogenicity of infectious bronchitis coronaviruses: complicated evolution and epidemiology in China caused by cocirculation of multiple types of infectious bronchitis coronaviruses Genetic diversity of avian infectious bronchitis coronavirus in recent years in China High-frequency RNA recombination of murine coronaviruses The molecular biology of coronaviruses Isolation and characterization of a novel antigenic subtype of infectious bronchitis virus serotype DE072 Stem-loop III in the 5 0 untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication Stem-loop IV in the 5 0 untranslated region is a cisacting element in bovine coronavirus defective interfering RNA replication Molecular characterization of avian infectious bronchitis virus strains from outbreaks in Argentina Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection An apparently new respiratory disease of chicks Evolutionary implications of avian infectious bronchitis virus (AIBV) analysis Unique and conserved features of genome and proteonome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and pathogenicity and evaluation of protection induced by Mass serotype H120 vaccine against strains of the LX4-type (QX) Differential stepwise evolution of SARS coronavirus functional proteins in different host species Evidence of natural recombination within the S1 gene of infectious bronchitis virus Evolutionary aspects of recombination in RNA viruses Adaptive evolution of the spike gene of SARS coronavirus: changes in positively selected sites in different epidemic groups Virus-encoded proteinases and proteolytic processing in the Nidovirales The autocatalytic release of a putative RNA virus transcription factor from its polyprotein precursor involves two paralogous papain-like proteases that cleave the same peptide bond Genetic interactions between an essential 3 0 cis-acting RNA pseudoknot, replicase gene products, and the extreme 3 0 end of the mouse coronavirus genome Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.meegid.2012. The etiology of IB, which was first demonstrated by Beach and Schalm (1936) , is infectious bronchitis virus (IBV).
- keywords: -utr; addition; amino; analysis; avian; bronchitis; cavanagh; chickens; china; complete; coronavirus; et al; events; evidence; evolution; field; flocks; gene; genome; genomic; h120; ibv; ibv strains; infectious; ldl/101212; length; lhlj/07vii; lhlj/100902; like; liu; lnm/091017; m41; mass; massachusetts; min; molecular; multiple; mutations; non; nucleotide; orfs; pathogenic; pcr; phylogenetic; primers; protein; recombination; respiratory; result; rna; sequences; serotype; spike; strain ck; strains; study; type; vaccinated; vaccine; viral; virus; viruses
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- cord-280442-jtvez46y
- author: Wu, Xuan
- title: Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick
- date: 2019-11-01
- words: 5316
- flesch: 47
- summary: NDV detection assays were specific to the NDV templates ( Figure 4B ). As for NDV detection assays, positive results were observed only when NDV templates existed.
- keywords: amplification; assays; avian; biotin; bronchitis; china; clades; clinical; control; conventional; copies; detecting; detection; diluted; disease; et al; figure; fitc; genome; higher; ibv; infectious; isothermal; lamp; lamp assays; lfd; line; lowest; mean; methods; mrt; multiple; ndv; newcastle; nrt; pathogens; pcr; pcr assays; positive; primers; products; qrt; rates; reaction; results; reverse; rna; samples; sensitive; sensitivity; single; specific; specificity; strains; studies; study; target; tcov; templates; test; time; transcription; virus; viruses
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- cord-281526-7t9e4lgn
- author: Yin, Lijuan
- title: Immunogenicity and protective efficacy of recombinant fusion proteins containing spike protein of infectious bronchitis virus and hemagglutinin of H3N2 influenza virus in chickens
- date: 2016-09-02
- words: 4537
- flesch: 40
- summary: After challenged with virulent IBV M41 strain, our results demonstrated that fusion proteins performed better protection compared with inactivated M41 vaccine and recombinant rS1 protein alone, while the latter groups presented similar protection ratio. The overall protein concentrations of recombinant proteins and inactivated IBV were determined using BCA protein assay kit (Pierce, Company); and the protein concentrations of the three recombinant proteins (rS1 protein, rS1-H3(TM) protein and rS1-HA2 protein) and S1 protein concentration of inactivated IBV were determined using SDS-PAGE gel electrophoresis by GeneSnap and GeneTools from SynGene software with BSA as standard.
- keywords: antibodies; antibody; avian; baculoviruses; better; booster; bronchitis; cavanagh; cd3; cd4; cd8; cells; cellular; challenge; chickens; collected; control; coronavirus; data; day; development; domain; efficacy; et al; fig; fusion; glycoprotein; group; h3(tm; h3n2; ha2; hemagglutinin; ibv; immune; immunity; immunization; immunogenicity; inactivated; increased; infectious; influenza; level; lymphocytes; m41; pbs; protection; protein; recombinant; responses; rs1; sf9; specific; spike; vaccinated; vaccination; vaccine; virus; weeks
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- cord-282314-9cua2jzg
- author: Albanese, Grace A.
- title: Biological and molecular characterization of ArkGA: A novel Arkansas serotype vaccine that is highly attenuated, efficacious, and protective against homologous challenge
- date: 2018-10-01
- words: 6383
- flesch: 46
- summary: In P60, SNPs were seen in S1 as well as S2 of the Table 2 S1 amino acid sequence comparison of ArkGA vaccine virus and viral RNA isolated from 5 choanal cleft palate swabs on days 7, 10, and 14 post-vaccination. However, it has been shown that the ArkDPI vaccine has an atypical vaccine coverage and replication pattern when mass applied by spray, and previous data from our laboratory suggests that the percentage of chicks infected with vaccine virus by 10 days post-vaccination only reaches 15-25%
- keywords: acid; additional; amino; analysis; ark99; arkansas; arkdpi; arkga; attenuated; avian; broiler; bronchitis; candidate; challenge; changes; chickens; chicks; ciliostasis; clinical; commercial; coronavirus; coverage; days; dose; eggs; eid; embryos; field; fig; following; gene; genome; group; ibv; infectious; isolated; isolation; load; non; old; p20; p40; p60; passages; pathogenic; pcr; position; post; previous; protection; protein; qrt; reduced; replication; research; rna; sequence; signs; snps; spike; spray; subpopulations; swabs; table; time; trial; type; vaccinated; vaccination; vaccine; viral; virus
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- cord-284501-5i0w74q4
- author: Armesto, Maria
- title: The Replicase Gene of Avian Coronavirus Infectious Bronchitis Virus Is a Determinant of Pathogenicity
- date: 2009-10-09
- words: 8008
- flesch: 47
- summary: Comparative analysis of complete genome sequences of three avian coronaviruses reveals a novel group 3c coronavirus Identification of a novel coronavirus from a beluga whale by using a panviral microarray Detection of a novel and highly divergent coronavirus from asian leopard cats and Chinese ferret badgers in Southern China Infectious bronchitis Avian coronavirus diseases and infectious bronchitis vaccine development Coronavirus avian infectious bronchitis virus Infectious Bronchitis Nidovirus genome organization and expression mechanisms Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence Heterologous gene expression from transmissible gastroenteritis virus replicon particles Engineering the transmissible gastroenteritis virus genome as an expression vector inducing lactogenic immunity Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis Severe acute respiratory syndrome coronavirus group-specific open reading frames encode nonessential functions for replication in cell cultures and mice Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity Effect of serial embryo passage of an Arkansas-type avian infectious bronchitis virus isolate on clinical response, virus recovery and immunity Attenuation, safety and efficacy of an infectious bronchitis virus GA98 serotype vaccine Development and use of the H strain of avian infectious bronchitis virus from The Netherlands as a vaccine: a review The pathogenesis of virulent and avirulent avian infectious bronchitis virus Characterization of a replicating and packaged defective RNA of avian coronavirus infectious bronchitis virus Replication and packaging of coronavirus infectious bronchitis virus defective RNAs lacking a long open reading frame Leader switching occurs during the rescue of defective RNAs by heterologous strains of the coronavirus infectious bronchitis virus Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41 Reverse genetics system for the avian coronavirus infectious bronchitis virus Taxonomic studies on strains of avian infectious bronchitis virus using neutralisation tests in tracheal organ cultures Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus A new DNA sequence assembly program Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus Open Reading Frames 3a and 3b of Infectious bronchitis virus cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging Replicase genes of murine coronavirus strains A59 and JHM are interchangeable: differences in pathogenesis map to the 39 one-third of the genome Altered pathogenicity, immunogenicity, tissue tropism and 39-7 kb region sequence of an avian infectious coronavirus strain after serial passage in embryos. A comparative sequence analysis to revise the current taxonomy of the family Coronaviridae Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: a review Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickens Complete genomic sequence of turkey coronavirus Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys Molecular identification and characterization of novel coronaviruses infecting graylag geese (Anser anser), feral pigeons (Columbia livia) and mallards (Anas platyrhynchos)
- keywords: accessory; acid; amino; analysis; avian; beaudette; bronchitis; cdna; cells; changes; chickens; chimaeric; ciliostasis; ck cells; clinical; coronavirus; corresponding; epithelial; fig; gene; genome; group; growth; ibv; ibv m41; infected; infectious; isolate; loss; m41; medium; nucleotide; observed; oligonucleotides; orf; passage; pathogenicity; pcr; post; potential; presence; protein; rbeaur; recombinant; recombination; region; rep; replicase; replicase gene; replication; responsible; results; ribv; rna; rvv; sequence; signs; strain; struct-2; structural; tocs; tracheas; utr; utrs; virulence; virulent; virus; viruses
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- cord-285052-aql0vrzv
- author: Kamble, Nitin Machindra
- title: Evolutionary and bioinformatic analysis of the spike glycoprotein gene of H120 vaccine strain protectotype of infectious bronchitis virus from India
- date: 2016-02-12
- words: 2822
- flesch: 47
- summary: The study identifies live attenuated IBV vaccine strain, which is routinely used for vaccination, for the first time. The nucleotide sequence of the S gene of Indian IBV vaccine strain was assembled and aligned by clustalW
- keywords: acid; aib; amino; analysis; antigenic; arg; available; bronchitis; cleavage; cross; different; disease; field; genbank; gene; glycoprotein; glycosylation; h120; homology; ibv; indian; infectious; isolates; kf188436; live; massachusetts; nucleotide; pcr; phylogenetic; polymerase; protection; protein; reported; sequence; sequencing; serotype; sites; spike; spike gene; strain; study; vaccination; vaccine; virus
- versions: original; plain text
- cord-285323-473d7zvg
- author: Jang, Hyesun
- title: Altered pro-inflammatory cytokine mRNA levels in chickens infected with infectious bronchitis virus
- date: 2013-09-01
- words: 4817
- flesch: 39
- summary: Interleukin-6 expression after infectious bronchitis virus infection in chickens Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells Proinflammatory cytokine response and viral replication in mouse bone marrow derived macrophages infected with influenza H1N1 and H5N1 viruses Newly isolated serotypes of infectious bronchitis virus: Their role in disease Avian Immunology Biologic basis for interleukin-1 in disease New insights on infectious bronchitis virus pathogenesis: Characterization of Italy 02 serotype in chicks and adult hens A choice of death-The signal-transduction of immune-mediated beta-cell apoptosis A novel chicken lung epithelial cell line: Characterization and response to low pathogenicity avian influenza virus Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens The TNF-TNF receptor system Antigenic differences among isolates of avian infectious bronchitis virus Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-alpha factor (LITAF) Susceptibility of three genetic lines of chicks to infection with a nephropathogenic T strain of avian infectious bronchitis virus Pro-inflammatory responses in chicken spleen and brain tissues after infection with very virulent plus Marek's disease virus Inflammatory cytokines and lipopolysaccharide induce Fas-mediated apoptosis in renal tubular cells Analysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR Genetic diversity of avian infectious bronchitis virus isolates in Korea between Live attenuated nephropathogenic infectious bronchitis virus vaccine provides broad cross protection against new variant strains Genetic diversity of avian infectious bronchitis coronavirus strains isolat A bovine herpesvirus type 1 mutant virus specifying a carboxylterminal truncation of glycoprotein E is defective in anterograde neuronal transport in rabbits and calves Genetic diversity of avian infectious bronchitis coronavirus in recent years in China IL-1b, TNF-a and IL-6 release from monocytes in haemodialysis patients in relation to dialytic age Rapid differentiation of current infectious bronchitis virus vaccine strains and field isolates in Australia Chemokine and cytokine gene expression profiles in chickens inoculated with Mycoplasma gallisepticum strains Rlow or GT5 challenge group inoculated with an isolate of the KIIa genotype (kr/ADL110002/2011, group 2), and challenge group inoculated with an isolate of the ChVI genotype dpi = days postinoculation AGP level in the serum (arithmetic mean ± SD) Therapy, Design of Vaccines, and Epidemiology Viral Immunol A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: Molecular cloning, sequencing, characterization, and chromosomal assignment Comparison of the susceptibility to avian infectious bronchitis virus infection of two inbred lines of White Leghorn chickens Re-emergence of fatal human influenza A subtype H5N1 disease H5N1 and 1918 pandemic influenza virus infection results in early and excessive infiltration of macrophages and neutrophils in the lungs of mice Infectious bronchitis virus: Immunopathogenesis of infection in the chicken Transcriptional profiling reveals a possible role for the timing of the inflammatory response in determining susceptibility to a viral infection Sequence analysis of the S1 glycoprotein of infectious bronchitis viruses Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and -susceptible chickens Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines Interferon and cytokine responses to SARS-coronavirus infection Pathology of fatal human infection associated with avian influenza A H5N1 virus Tumor necrosis factor and interleukin-1 induce expression of the verocytotoxin receptor globotriaosylceramide on human endothelial cells: Implications for the pathogenesis of the hemolytic uremic syndrome Viral diseases of respiratory system Rapid expression of chemokines and proinflammatory cytokines in newly hatched chickens infected with Salmonella enterica serovar Typhimurium Plasma inflammatory cytokines and chemokines in severe acute respiratory syndrome
- keywords: acute; adl110002/2011; agp; analysis; avian; bronchitis; cells; challenged; chickens; chvi; clinical; control; copy; cytokines; different; disease; dpi; et al; excessive; expression; factor; figure; gene; genotype; glycoprotein; group; ibv; il-6; immunopathogenesis; increase; infected; infection; inflammatory; inoculated; isolate; kidney; kiia; levels; litaf; necrosis; number; pcr; postinoculation; pro; production; respiratory; response; rna; serum; significant; signs; study; tnf; trachea; transcription; viral; virus
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- cord-285330-td4vr0zv
- author: Mohammadi, Ali
- title: Viral quantity and pathological changes in broilers experimentally infected by IRFIBV32 isolate of infectious bronchitis virus
- date: 2015-11-12
- words: 3162
- flesch: 53
- summary: Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain Studies on avian viral infections in Iran Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos Re-excretion of infectious bronchitis virus in chickens induced by cyclosporin Pathogenesis and tissue distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/b serotype) in experimentally infected broiler chickens Isolation and identification of a new isolate of avian infectious bronchitis virus IRFIBV32 and a study of its pathogenicity Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Infectious bronchitis Detection of infectious bronchitis virus strain N1/88 from the oviduct and feces of experimentally infected vaccinated and unvaccinated hens LNA probe-based real-time RT-PCR for the detection of infectious bronchitis virus from the oviduct of unvaccinated and vaccinated laying hens Detection the 4/91 strain of infectious bronchitis virus in testicular tissue from experimentally infected rooster by reverse transcription-polymerase chain reaction Virulent avian infectious bronchitis virus, People's Republic of China Pathogenicity of infectious bronchitis virus isolates from Ontario chickens Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the beaudette strain The immunohistochemistry study of lesions due to avian infectious bronchitis (serotype 4/91) on different tissues in specific pathogen free chicks Establishment of persistent avian infectious bronchitis virus infection in antibodyfree and antibody-positive chickens A simple method of estimating fifty percent endpoint Isolation and identification of infectious bronchitis viruses in chickens in Iran Viral quantity and pathological changes in broilers experimentally infected by IRFIBV32 isolate… 323 Acknowledgments The authors extend thanks to the School of Veterinary Medicine, Shiraz University, for providing the necessary facilities and financial support to carry out this study. This disease is caused by infectious bronchitis virus (IBV), a member of the family Coronaviridae (order Nidovirales) and
- keywords: avian; birds; bronchitis; bronchitis virus; caecal; cells; changes; chickens; chicks; days; detection; different; dpi; group; ibv; infected; infectious; infectious bronchitis; irfibv32; isolate; kidneys; lesions; load; lungs; maximum; organs; ovaries; pcr; probe; replication; rna; rrt; samples; strain; study; testes; time; tissues; titer; tonsils; trachea; viral; virus
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- cord-285942-mb1xwdqw
- author: Feng, K. Y.
- title: Molecular characteristic and pathogenicity analysis of a virulent recombinant avain infectious bronchitis virus isolated in China
- date: 2018-10-01
- words: 6890
- flesch: 52
- summary: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection Cytotoxic T lymphocytes are critical in the control of infectious bronchitis virus in poultry The long view: 40 years of infectious bronchitis research A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B) Epidemiology and characterization of avian infectious bronchitis virus strains circulating in southern China during the period from 2013-2015 Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during Complete genomic sequence of turkey coronavirus A 'new' strain of infectious bronchitis virus infecting domestic fowl in Great Britain A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens Immune responses to structural proteins of avian infectious bronchitis virus Review of infectious bronchitis virus around the world A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Evidence of genetic diversity generated by recombination among avian coronavirus IBV Serotype and genotype diversity of infectious bronchitis viruses isolated during 1985 Complete genome sequence analysis of a predominant infectious bronchitis virus (IBV) strain in China Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648 A New Model for Coronavirus Transcription Adoptive transfer of infectious bronchitis virus primed alphabeta T cells bearing CD8 antigen protects chicks from acute infection Role of nucleotides immediately flanking the transcriptionregulating sequence core in coronavirus subgenomic mRNA synthesis Recombination in avian gamma-coronavirus infectious bronchitis virus Evidence of natural recombination within the S1 gene of infectious bronchitis virus Genome sequence and origin analyses of the recombinant novel IBV virulent isolate SAIBK2 Complete genome sequence of a recombinant nephropathogenic infectious bronchitis virus strain in china Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination Analysis of a QX-like avian infectious bronchitis virus genome identified recombination in the region containing the ORF 5a, ORF 5b, and nucleocapsid protein gene sequences Coronavirus nucleocapsid protein facilitates template switching and is required for efficient transcription Sequence comparison among QY16 and other IBV strains was conducted and its results demonstrate that the S1 gene of QY16 has the highest nucleotide sequence identity with that of 4/91, and the other part of its genome is highly similar to that of YX10.
- keywords: analysis; arg; avian; bronchitis; bronchitis virus; challenge; chickens; china; chinese; comparison; complete; complete genome; coronavirus; different; encodes; et al; figure; gene; genome; group; h120; highest; ibv; ibv strains; identity; infectious; infectious bronchitis; inoculated; insertion; isolated; nucleotide; parental; pathogenicity; pcr; phylogenetic; post; poultry; protection; protein; qy16; rate; rdp; recombinant; recombination; reference; region; replication; results; rna; s1 gene; sequence; serotypes; similar; specific; spike; strains; structural; study; supplementary; table; transcription; trs; type; vaccinated; vaccination; vaccine; viral; virus; yx10
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- cord-286332-cdg4im5h
- author: van Beurden, Steven J.
- title: A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination
- date: 2017-06-12
- words: 7008
- flesch: 49
- summary: The most important findings were that (1) murinized IBV contained the correct 5′ S gene sequence (Primer set [F] -with a forward primer located in the IBV 1b gene at a position upstream of the 1b sequence present in p-mIBV and a reverse primer located in the MHV spike gene -resulted in a detectable PCR product for mIBV, but not for IBV or p-mIBV); (2) murizined IBV contained the MHV spike gene at the location of the IBV spike gene (as both primer set [F] and [H] -each with a primer in the MHV spike gene and one in IBV gene 1b [F] or in the M gene [H] -resulted in a detectable PCR product for mIBV, but not for IBV or MHV); (3) the IBV spike gene was absent from the mIBV genome (as primer set [D] targeting the IBV spike gene resulted in a detectable PCR product for IBV, but not for mIBV or MHV(4); recombinant IBV was the result of recombination between genomic RNA from mIBV and RNA transcribed from p-IBV (as primer set [C] -with a forward primer in IBV gene 1b located upstream of the 1b sequence present in p-mIBV and a reverse primer located in the IBV spike gene -resulted in a detectable PCR product for rIBV-wt, but not for mIBV or p-IBV); (5) recombinant IBV contained the IBV spike gene at the location of the MHV-derived spike in mIBV (as both primer set [C] and primer set [E] -each with one primer in the IBV spike gene and the other in IBV ORF
- keywords: accessory; additional; allantoic; anti; avian; beaudette; bronchitis; cells; chickens; chimeric; cloning; coronavirus; culture; day; days; death; dilution; donor; eces; ectodomain; eggs; embryonic; enzyme; fig; gene; generation; genetics; genome; h52; host; ibv; ibv h52; ibv spike; infected; infectious; inoculated; lr7; lr7 cells; manipulation; mhv; mibv; mouse; mutations; netherlands; old; ovo; p.i; parental; pcr; plasmid; primer; protein; recombinant; recombination; region; replication; restriction; reverse; ribv; rna; sequence; species; specific; spike; spike gene; step; strain; structural; subsequent; synthetic; system; table; targeted; tropism; type; usa; viral; virus; viruses; wild
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- cord-286473-sl5zy8nj
- author: Gomaa, M.H.
- title: Complete genomic sequence of turkey coronavirus
- date: 2008-05-12
- words: 7986
- flesch: 54
- summary: Similar results were obtained with a large number of IBV sequences and five TCoV sequences with the M protein (data not shown). The 3 end of TCoV genome had been done using the 3 race system for rapid amplification of cDNA ends (Invitrogen) according to the instruction of the kite.
- keywords: acids; aligned; amino; analysis; available; avian; bcov; bronchitis; cdna; cleavage; coding; conserved; coronaviruses; data; deletion; downstream; end; enteritis; envelope; et al; fig; functional; gene; genome; genomic; glycoprotein; group; hcov; hcov-229e; high; ibv; identity; iii; infectious; invitrogen; isolates; length; like; maximum; membrane; mg10; motif; n gene; nucleocapsid; nucleotides; orf; orf1b; orfs; organization; pcr; polyprotein; potential; presence; primer; products; protein; putative; region; relationships; replication; respiratory; rna; role; sars; search; sequence; similar; sites; small; spike; strains; structure; study; syndrome; table; tcov; tcov genome; terminal; trees; trs; turkey; upstream; utr
- versions: original; plain text
- cord-286658-9kco7qad
- author: Jiang, Lei
- title: Characterization of the complete genome, antigenicity, pathogenicity, tissue tropism, and shedding of a recombinant avian infectious bronchitis virus with a ck/CH/LJL/140901-like backbone and an S2 fragment from a 4/91-like virus
- date: 2018-01-15
- words: 7028
- flesch: 46
- summary: The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China The avian coronavirus infectious bronchitis virus undergoes direct low-pH-dependent fusion activation during entry into host cells The long view: 40 years of infectiousbronchitis research Sequence and structure of the coronavirus peplomer protein Cooperative involvement f the S1 and S2 subunits of the murine coronavirus spike protein in receptor binding and extended host range Infectious bronchitis virus variants: a review of the history, current situation and control measures Recommendations for a standardized avian coronavirus (AvCoV) nomenclature: outcome from discussions within the framework of the European Union COST Action FA1207: towards control of avian coronaviruses: strategies for vaccination, diagnosis and surveillance Molecular investigation of a full-length genome of a Q1-like IBV strain isolated in Italy in 2013 Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein Genetic, antigenic, and pathogenic characteristics of avian infectious bronchitis viruses genotypically related to 793/B in China Molecular and serologic characterization pathogenicity, and protection studies with infectious bronchitis virus field isolates from California Molecular evolution and emergence of avian gammacoronaviruses Genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south China Antigenic domains on thepeplomer protein of avian infectious bronchitis virus: correlation with biologicalfunctions Phylogeny of antigenic variants of avian coronavirus IBV Full-length genome sequencing analysis of avian infectious bronchitis virus isolate associated with nephropathogenic infection Antigenicity of the peplomer protein of infectious bronchitis virus infectious bronchitis virus isolates in southern china A novel variant of the infectious bronchitis virus resulting from recombination events in Italy L Mulan: multiple-sequence local alignment and visualization for studying function and evolution Contributions of the S2 spike ectodomain to attachment and host range of infectious bronchitis virus Full genome analysis of Australian infectious bronchitis viruses suggests frequent recombination events between vaccine strains and multiple phylogenetically distant avian coronaviruses of unknown origin A simple method of estimating fifty percent endpoints Why do RNA viruses recombine? Recombination in avian gamma-coronavirus infectious bronchitis virus S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification Australian infectious bronchitis viruses: identification of nine subtypes by a neutralisation test Binding of avian coronavirus spike proteins to host factors reflects virus tropism and pathogenicity Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination Analysis of a QX-like avian infectious bronchitis virus genome identified recombination in the region containing the ORF 5a, ORF 5b, and nucleocapsid protein gene sequences Origin and evolution of LX4 genotype infectious bronchitis coronavirus in China Complete genome sequence of a novel infectious bronchitis virus strain circulating in China with a distinct S gene Identification of a novel recombinant virulent avian infectious bronchitis virus This work was supported by grants from the China Agriculture Research Systerm (No. CARS-40-K18) and National Twelfth Five-Year Plan for Science and Technology Support (2015BAD12B03).
- keywords: acid; addition; amino; analysis; antigenic; avian; birds; bronchitis; bronchitis virus; challenge; chen; chickens; china; clinical; complete; coronavirus; days; eggs; end; epitopes; et al; events; fig; genbank; gene; genome; i1101/16; i1101/16 isolate; ibv; ibv strains; infectious; infectious bronchitis; isolate; like; liu; ljl/140901; ljl/140901 strain; neutralization; novel; nucleotide; old; post; protein; recombinant; recombination; reference; reference strains; region; relatedness; replication; results; sequences; serotype; shedding; signs; spf; spike; spike protein; strain; strain ck; subunit; table; trachea; type; vaccine; viral; virus; viruses
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- cord-288309-6pw7t512
- author: Kusters, J. G.
- title: Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus
- date: 1990-12-31
- words: 1973
- flesch: 57
- summary: virus retaining spike glycopolypeptide S2 but not $1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection Eyidence for a coiled-coil structure in the spike protein of coronaviruses Coronavirus proteins: biogenesis of avian bronchitis virus virion proteins Phylogeny of antigenic variants of avian coronavirus IBV Occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the Netherlands Comparison of the spike precursor sequences of coronavirus IBV strains M41 and 6•82 with that of IBV Beaudette Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains A comprehensive sequence analysis program for the IBM personal computer Confidence limits on phylogenies: an approach using the bootstrap Highfrequency RNA recombination of murine coronaviruses In vivo RNA-RNA recombination of coronavirus in mouse brain Cloning and sequencing of the gene encoding the spike protein of coronavirus IBV Evolution of avian coronavirus IBV: sequence of the matrix glycoprotein gene and intergenic region of several serotypes The tree based on S1 gene sequences (I in Figure 2b ) has the same topology as an $2 tree based on region II in $2. KB8523 has shifted to the D1466 branch in a tree for region III of $2.
- keywords: 6/82; amino; arg; avian; bronchitis; coronavirus; crossover; d1466; d207; data; field; figure; frequency; gene; ibv; infectious; kb8523; new; nucleotide; phylogenetic; protein; recombination; region; rna; sequences; serotypes; site; spike; strains; tree; vaccines; virus
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- cord-290638-7ro72sv3
- author: Lenstra, Johannes A.
- title: Antigenicity of the peplomer protein of infectious bronchitis virus
- date: 1989-01-31
- words: 3968
- flesch: 46
- summary: After adding concentrated SDS-containing lysis buffer (Laemmli and Favre, 1973) , hybrid proteins were spotted via a manifold on nitrocellulose membranes or fractionated by SDSpolyacrylamide gel electrophoresis (7.5%) followed by Western blotting. Hybrid proteins thus immobilized were analysed as described for the filters of the immunoscreening (see above).
- keywords: antibodies; antigenic; antigenicity; antisera; antiserum; binding; bronchitis; chickens; clones; conformation; different; dna; dnase; epitopes; et al; expression; fig; fragments; gene; hybrid; ibv; infectious; koch; localization; m41; mab; monoclonal; neutralization; neutralizing; number; peplomer; peptides; pex; phosphatase; polyclonal; products; protein; rabbit; reading; region; residues; restriction; results; sequence; stanley; strain; subunit; terminal; unpublished; vaccines; virus; western
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- cord-291174-rym84kni
- author: Yang, Yazhi
- title: A label-free electrochemical assay for coronavirus IBV H120 strain quantification based on equivalent substitution effect and AuNPs-assisted signal amplification
- date: 2020-10-23
- words: 3587
- flesch: 45
- summary: In this electrochemical method, H120 strain detection is based on equivalent substitution effect and AuNPs-assisted signal amplification with a detection range from 1.56e −9 to 1.56e −6 μM. Compared with previously reported IBV detection methods that may focus on the portable and simplified analysis, our electrochemical assay still has some advantages. The proposed method shows a detection range of 1.56e(−9) to 1.56e(−6) μM with the detection limit of 2.96e(−10) μM for IBV H120 strain selective quantification detection, exhibiting good accuracy, stability, and simplicity, which shows a great potential for IBV detection in vaccine research and avian infectious bronchitis diagnosis.
- keywords: amplification; assay; aunps; avian; bronchitis; buffer; concentration; copy; coronavirus; detection; different; dna; electrochemical; electrode; enzyme; fig; gene; gold; great; h120; hcl; ibv; identification; infectious; lane; linear; method; min; nanoparticles; nna; pcr; peak; plasmid; probe; quantification; reaction; real; response; rna; room; ruhex; sequence; signal; solution; standard; strain; strand; surface; table; target; temperature; tris; ultrasensitive; virus; ° c
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- cord-291510-jh2fdks4
- author: Jiang, Yi
- title: Recombinant infectious bronchitis coronavirus H120 with the spike protein S1 gene of the nephropathogenic IBYZ strain remains attenuated but induces protective immunity
- date: 2020-02-11
- words: 8033
- flesch: 46
- summary: Sheng Zhou: Conceptualization, Validation, Resources, Writing -original draft The long view: 40 years of infectious bronchitis research Coronaviruses in poultry and other birds Review of infectious bronchitis virus around the world Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Coronavirus avian infectious bronchitis virus Infectious bronchitis virus variants: molecular analysis and pathogenicity investigation Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus Progress and challenges toward the development of vaccines against avian infectious bronchitis A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China Genetic diversity of avian infectious bronchitis coronavirus in recent years in China Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area Current status of veterinary vaccines Protection of laying hens against infectious bronchitis with inactivated emulsion vaccines Avian infectious bronchitis: the protection afforded by an inactivated virus vaccine IgM responses in chicken serum to live and inactivated infectious bronchitis virus vaccines Molecular characterization and pathogenicity of infectious bronchitis coronaviruses: complicated evolution and epidemiology in china caused by cocirculation of multiple types of infectious bronchitis coronaviruses Molecular characterization of an infectious bronchitis virus strain isolated from northern China in 2012 Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine Attenuation, safety, and efficacy of a QX-like infectious bronchitis virus serotype vaccine Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine Rapid development and evaluation of a live-attenuated QX-like infectious bronchitis virus vaccine Efficacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens Safety and efficacy of an infectious bronchitis virus used for chicken embryo vaccination Embryo vaccination of chickens with infectious bronchitis virus: histologic and ultrastructural lesion response and immunologic response to vaccination Attenuated vaccines can recombine to form virulent field viruses Recombinant infectious bronchitis viruses expressing chimeric spike glycoproteins induce partial protective immunity against homologous challenge despite limited replication in vivo Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens Recombinant infectious bronchitis viruses expressing heterologous S1 subunits: potential for a new generation of vaccines that replicate in Vero cells Reverse genetics system for the avian coronavirus infectious bronchitis virus Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120 Infectious bronchitis virus as a vector for the expression of heterologous genes The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens Construction of an infectious fulllength cDNA clone of infectious bronchitis virus H120 vaccine strain Expression of green fluorescent protein using an infectious cDNA clone of infectious bronchitis virus The causative agent of IB is infectious bronchitis virus (IBV), a non-segmented, positive-sense, single-stranded RNA virus, which belongs to the genus Gammacoronavirus, family Coronaviridae, in the order Nidovirales
- keywords: antibodies; antibody; attenuated; avian; birds; bronchitis; bronchitis virus; cells; challenge; chickens; china; clinical; cloacal; collected; control; coronavirus; data; day; different; dpi; efficacy; eggs; eid; embryos; fig; gene; genetic; group; growth; host; ibv; infected; infectious; infectious bronchitis; kidney; lesions; level; like; live; load; lower; lungs; mortality; nephropathogenic; old; pcr; poultry; protection; protein; recombinant; replication; respiratory; reverse; rh120; rh120 group; rh120 strain; ribyz; ribyz strain; rna; s1 gene; samples; significant; signs; spf; spike; standard; strain; study; system; time; tissue; trachea; tropism; vaccinated; vaccination; vaccine; viral; virulent; virus; yz group; yz strain
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- cord-291718-cz1bi0ym
- author: Yu, Liping
- title: The papain-like protease of avian infectious bronchitis virus has deubiquitinating activity
- date: 2017-03-18
- words: 3488
- flesch: 49
- summary: Further investigations are necessary to determine if IBV PLP DUB activity inhibits the host antiviral response. Further experiments indicated that the proteinase PLP-TM plays an important role in IBV DUB activity and can process both K48-and K63-linked polyubiquitin chains.
- keywords: a59; activity; antiviral; avian; bronchitis; catalytic; cells; chains; cleavage; coronavirus; covs; df1; domain; dub; dub activity; fig; host; ibv; ifn; important; infection; innate; k48; k63; levels; like; mutants; orf; papain; plp; plp2; polyubiquitin; prk5; protease; proteins; replication; role; study; type; ubiquitin; ubiquitinated; virus
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- cord-291754-1zxztadu
- author: Zhao, Ye
- title: Successful establishment of a reverse genetic system for QX-type infectious bronchitis virus and technical improvement of the rescue procedure
- date: 2019-10-15
- words: 6873
- flesch: 43
- summary: The cDNA samples were submitted for SYBR Green I real-time RT-qPCR to detect the viral load for IBV N gene as described before (Zhao et al., 2015) . To compare the in vitro replication of the rescued virus rYN and its parental strain YN on CEK cells, 200 μl PBS containing 10 3.0 TCID 50 of rYN or YN virus were inoculated onto the CEK cells in 24-well plates, and 200 μl supernatants from three wells from each group were harvested at the time points of 6, 12, 24, 36, 48, and 60 hpi for a real-time PCR detection assay for IBV N gene as described above.
- keywords: allantoic; avian; bamhi; bhk-21; bronchitis; bronchitis virus; cdna; cek; cells; chicken; china; cloned; complete; coronavirus; day; ece; egfp; eggs; eid; electroporation; embryo; et al; expression; fig; fluid; fluorescence; fragment; gene; genetics; genome; ibv; incubation; infectious; inoculated; length; lines; ovo; parental; pathogenicity; pcr; pgpt-1; plasmid; plates; procedure; promoter; protein; recombinant; recombination; replication; rescue; restriction; reverse; rna; ryn; selection; sequence; site; strain; study; system; tcid; time; transcription; type; vaccinia; vaccinia virus; vector; viral; virus; viruses; vitro; vvibv; zhao
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- cord-292428-j3wdlpp6
- author: Zhou, J.‐Y.
- title: Characterization of an Avian Infectious Bronchitis Virus Isolated in China from Chickens with Nephritis
- date: 2004-06-30
- words: 3759
- flesch: 40
- summary: The PCR products of S1 gene were approximately 1.93 kb in size, which was consistent with expected S1 gene (Fig. 3) . Unfortunately, up to now, serotypes of the different tissue tropism of field IBV isolates were not identified using virus neutralization test or monoclonal antibody.
- keywords: acid; allantoic; amino; avian; birds; bronchitis; chickens; china; coronavirus; eggs; embryos; field; fig; flocks; fluid; gene; ibv; infected; infectious; inoculated; inoculation; isolate; isolate sc021202; isolation; kidney; lesions; mortality; nephritis; nephropathogenic; nucleotide; pcr; protein; renal; respiratory; rna; s1 gene; sc021202; sequence; serotype; signs; spf; strains; suspension; tissue; virus; zhou
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- cord-293081-40pa5g89
- author: Li, Jun
- title: Preliminary crystallographic analysis of avian infectious bronchitis virus main protease
- date: 2006-12-16
- words: 1515
- flesch: 54
- summary: In this report, we describe the crystallization and preliminary crystallographic analysis of IBV M pro as a prelude to the elucidation of its three-dimensional structure in order to provide a basis for rational drug design. The cDNA encoding IBV M pro (M41 strain) was a gift from Professor Ming Liao (South China Agricultural University, People's Republic of China).
- keywords: anand; bronchitis; cacodylate; cavanagh; coronavirus; crystallization; crystals; data; et al; group; gst; holmes; ibv; infection; main; peg; pro; propanol; protease; protein; purified; respiratory; sodium; solution
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- cord-293651-96cmduez
- author: Callison, Scott A.
- title: Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens
- date: 2006-08-28
- words: 4749
- flesch: 48
- summary: In this study, we developed a TaqMan ®based real-time RT-PCR assay for rapid detection of IBV viral RNA directly from clinical samples. In this report we present the development and evaluation of a real-time Taqman ® -based RT-PCR assay for the detection and quantification of IBV genomic RNA directly from tracheal swabs.
- keywords: age; antibodies; assay; avian; birds; bronchitis; calculated; chickens; clinical; commercial; control; copies; curve; d.p.i; days; detection; diagnostic; different; disease; dose; fig; flock; genome; group; ibv; important; infectious; isolation; jackwood; limit; load; negative; number; pcr; pcr assay; positive; post; probe; quantification; reaction; real; rna; samples; specific; standard; strains; swabs; template; test; time; time rt; total; tracheal; vaccination; viral; virus
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- cord-294679-7pklrmz5
- author: Wei, Lei
- title: Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain
- date: 2008-08-09
- words: 1071
- flesch: 54
- summary: The structural and functional analysis of IBV nsp3 ADRP domain will be published elsewhere. The gene encoding the IBV nsp3 # 2008 International Union of Crystallography All rights reserved ADRP domain (corresponding to Asp1024-Lys1178 of the ORF1a polyprotein) was inserted between the BamHI and XhoI sites of the pGEX-6p-1 plasmid.
- keywords: adp; adrp; avian; bronchitis; coronaviruses; crystallization; crystals; domain; genome; ibv; infectious; nsp3; nsps; protein; solution; virus
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- cord-295312-68b3zio6
- author: Britton, Paul
- title: Genes 3 and 5 of Infectious Bronchitis Virus are Accessory Protein Genes
- date: 2006
- words: 1109
- flesch: 36
- summary: We have rescued a rIBV that lacks expression of 3a and 3b, after deletion of their sequences, and lacks expression of 5a and 5b after scrambling of the gene 5 TAS indicating that both sets of gene products are dispensable in vitro. Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus A nomenclature for avian coronavirus isolates and the question of species status Diseases of Poultry Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus The Coronaviridae The molecular biology of coronaviruses The Coronaviridae A polycistronic mRNA specified by the coronavirus infectious bronchitis virus Identification of a new membrane associated polypeptide specified by the coronavirus infectious bronchitis virus Internal entry of ribosomes on a tricistronic mRNA encoded by infectious bronchitis virus Distinct structural elements and internal entry of ribosomes in mRNA3 encoded by infectious bronchitis virus Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection Reverse genetics system for the avian coronavirus infectious bronchitis virus Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity Transient dominant selection of recombinant vaccinia viruses Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickens Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys [1] [2] [3] Interspersed amongst the IBV structural protein genes are two genes, 3 and 5 ( Fig. 1 ), 4 whose role is unknown.
- keywords: avian; bronchitis; cells; coronavirus; gene; ibv; infectious; initiation; modified; proteins; recombinant; replication; sequences; virus
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- cord-296167-np0b9a7o
- author: Mardani, Karim
- title: Naturally occurring recombination between distant strains of infectious bronchitis virus
- date: 2010-06-24
- words: 2965
- flesch: 44
- summary: Analysis of the S gene sequences of Australian IBV strains showed that, among classical strains, they differed by 3-12.5% and, among novel strains, by 3.2-16.4%. Evolutionary relationships between Australian IBV genes were inferred using the neighbour-joining method [24] .
- keywords: analysis; australia; avian; bronchitis; characterization; classical; classical ibvs; coronavirus; different; emergence; evolutionary; fig; gene; genome; groups; ibvs; infectious; informative; method; n1/03; new; novel; novel ibvs; number; pcr; phylogenetic; reaction; recombination; region; rna; sequences; similarity; sites; strains; study; variant; virus; viruses
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- cord-296364-7rp60d2m
- author: Youn, Soonjeon
- title: In vitro assembled, recombinant infectious bronchitis viruses demonstrate that the 5a open reading frame is not essential for replication
- date: 2005-02-05
- words: 5114
- flesch: 50
- summary: The transcripts from the full-length cDNA (along with the N transcript alone), total cellular RNA from IBV infected Vero cells (positive control), and PBS (negative control) were electroporated into non-permissive BHK-21 cells, which were then cultured with Vero cells. The entire IBV N ORF, including the 3V UTR, was amplified by RT-PCR from total cellular RNA extracted from IBV infected Vero cells, and the RT-PCR product was cloned into a transcription vector, pGEM-3Zf(+) (Promega, Madison, WI).
- keywords: according; amplicon; assembly; beaudette; bronchitis; bsmbi; cdna; cells; cloned; cloning; control; coronavirus; cpe; deletion; egfp; entire; essential; et al; expression; fig; fragments; gene; genome; gibv; group; growth; ibv; infectious; length; mhv; mibv; molecular; nucleotide; orf; pcr; plaques; product; protein; recombinant; region; replication; restriction; reverse; rna; sequence; sequencing; size; smaller; specific; standard; strain; strategy; template; transcription; vectors; vero; viral; virus; viruses
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- cord-296524-68gwusfe
- author: BARR, DA
- title: Isolation of infectious bronchitis virus from a flock of racing pigeons
- date: 2008-03-10
- words: 784
- flesch: 58
- summary: We report the isolation of IBV from a flock of racing pigeons and assess its significance. This response is common following vaccination of chickens, whereas wild IBV stimulate higher antibody levels.
- keywords: affected; allantoic; antibody; birds; chickens; days; disease; eggs; faragher; flock; ibv; inoculation; levels; pigeons; racing
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- cord-296611-ma32oz4o
- author: Ma, Tianxin
- title: Novel genotype of infectious bronchitis virus isolated in China
- date: 2019-01-29
- words: 5923
- flesch: 42
- summary: Phylogenetic and evolutionary relationships among torovirus field variants: evidence for multiple intertypic recombination events S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks Genetic diversity of avian infectious bronchitis virus in China in recent years Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens Sequencing and serologic identification of S1 genes of infectious bronchitis viruses isolated during 2012-2013 in Guangxi Province, China The authors declare that they have no competing interests. Infection with strain LGX/111119 resulted in significantly higher production of infectious viruses in trachea, lung, kidney, proventriculus, and cecal tonsil tissues than those of corresponding tissues of chickens infected with strain I0636/16 (Fig. 4B) .
- keywords: acid; allantoic; amino; analysis; antigenic; avian; birds; bronchitis; bronchitis virus; cecal; challenge; chickens; china; complete; coronavirus; day; different; eggs; emergence; end; et al; events; fig; gene; genome; genomic; genotype; gi-28; gvii-1; i0636/16; ibv; ibv strains; infectious; infectious bronchitis; kidney; lgx/111119; lineages; liu; new; nn130021; novel; nucleotide; old; parental; pathogenicity; phylogenetic; positive; post; province; recombination; reference; region; replication; representative; respiratory; results; s1 gene; sequence; serotype; spf; spike; strain; strain i0636/16; study; trachea; tract; virus; viruses
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- cord-296831-wdpatr2z
- author: Matoo, Javaid Jeelani
- title: Resiquimod enhances mucosal and systemic immunity against avian infectious bronchitis virus vaccine in the chicken
- date: 2018-04-07
- words: 5023
- flesch: 50
- summary: Further, the combination of R-848 withinactivated IBV vaccine showed higher antibody response than the vaccine alone group. Interestingly, it potentiated the IgA responses in the tear and intestinal secretions when used with both live and inactivated IBV vaccines.
- keywords: absence; adjuvant; administration; age; agonist; analysis; antibody; antigen; avian; birds; booster; bronchitis; cells; chicken; control; data; difference; dpi; effect; enhanced; experiment; fig; group; higher; ibv; ibv vaccine; iga; immune; immunity; immunization; inactivated; increase; infectious; intestinal; like; live; mean; mucosal; n =; pbmcs; post; presence; r-848; resiquimod; response; significant; specific; spf; tear; test; tgf; time; transcripts; treatment; usa; vaccination; vaccine; virus
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- cord-298078-uqrwq5qk
- author: Kwak, Hoyun
- title: Annexin A2 Binds RNA and Reduces the Frameshifting Efficiency of Infectious Bronchitis Virus
- date: 2011-08-30
- words: 5377
- flesch: 51
- summary: Since the frameshifting of IBV generates RNA dependent RNA polymerase, which is critical for successful viral replication, the protein that inhibits frameshifting must be a major antiviral regulator in eukaryotic cells. Since translation is a complex process involving many regulatory factors in addition to the ribosome, binding of RNA binding protein (RBP) at a nearby RNA signal could affect RNA conformation and somehow redirect the translational machinery
- keywords: activity; analysis; annexin; anti; antibody; anxa2; assay; bands; beads; binding; blot; buffer; cells; cellular; coding; complexes; control; coronavirus; data; efficiency; expression; extracts; figure; flag; frameshifting; gel; gene; gst; hek293; hepes; human; ibv; ibv pseudoknot; imr90; knockdown; lncap; luciferase; min; mrna; mutant; pcr; protein; pseudoknot; pseudoknot rna; region; regulation; relative; replication; reporter; ribosomal; ribosome; rna; rnase; role; sequences; signal; sirna; specific; structure; tof; translation; type; vector; viral; virus; western; wild
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- cord-298920-1lc2xf7u
- author: Bello-Perez, Melissa
- title: Canonical and Noncanonical Autophagy as Potential Targets for COVID-19
- date: 2020-07-05
- words: 6924
- flesch: 34
- summary: In fact, coronaviruses can both induce and inhibit autophagy with interactions at multiple levels within a narrow action area limited by apoptosis and the IFN response. The most contradictory fact, given the close interaction between coronaviral replication and autophagy pathways, is that the knockout of genes encoding autophagy-essential proteins such as ATG5, ATG7, BECN1 or LC3 di not abolish the replication in cell culture of the coronaviruses tested; actually, for many of them, replication was unaffected or increased ( Table 3 ).
- keywords: activation; activity; akt; alternative; apoptosis; atg5; atg7; autophagosome; autophagy; becn1; canonical; cells; cellular; chloroquine; chop; complex; coronavirus; cov; cov-2; cycle; degradation; different; double; drugs; effect; eif2ak3; elements; endoplasmic; entry; essential; fact; factor; figure; formation; fusion; golgi; host; ibv; immune; independent; induced; infected; infection; inhibition; ire1; jnk; kinase; lc3; lysosomes; machinery; mapk; mechanism; membrane; mers; molecular; mtor; new; novel; nsp6; omegasome; pathway; perk; phagophore; phosphatidylinositol; process; protein; receptor; recruitment; regulated; regulation; replication; respiratory; response; reticulum; sars; selective; sense; signaling; specific; stages; starvation; stress; studies; syndrome; targets; treatment; upr; vesicles; viral; viruses
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- cord-299428-gon6bzat
- author: Mondal, Shankar
- title: Sequence analysis of infectious bronchitis virus isolates from the 1960s in the United States
- date: 2012-10-11
- words: 3278
- flesch: 50
- summary: The BlastN program (http:// www.ncbi.nlm.nih.gov/BLAST/) was used to search GenBank for homologous IBV S1 and N gene sequences. Complete nucleotide analysis of the structural genome of the infectious bronchitis virus strain md27 reveals its mosaic nature Veterinary Microbiology Coronavirus avian infectious bronchitis virus Infectious Bronchitis Family Coronaviridae Infectious bronchitis virus types: incidence in the United States The evolution and emergence of RNA viruses Serologic and immunologic properties of a recent isolate of infectious bronchitis virus Recombinational histories of avian infectious bronchitis virus and turkey coronavirus Immune responses to structural proteins of avian infectious bronchitis virus Genetic and antigenic diversity in avian infectious bronchitis virus isolates of the 1940s Immunological differences in strains of infectious bronchitis virus Serotype identification of avian infectious bronchitis virus by RT-PCR of the peplomer (S1) gene Molecular cloning and sequence comparison of the S1 glycoprotein of the Gray and JMK strains of avian infectious bronchitis virus Redesign of primer and application of the reverse transcriptase-polymerase chain reaction and restriction fragment length polymorphism test to the DE072 strain of infectious bronchitis virus S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea Phylogenetic analysis of avian infectious bronchitis virus strains isolated in Japan Sequence analysis of the S1 glycoprotein of infectious bronchitis viruses: identification of a novel genotypic group in Australia An apparently new respiratory disease of chicks Complete nucleotide sequences of S1 and N genes of infectious bronchitis virus isolated in Japan and Taiwan Infectious bronchitis virus variants: a review of the history, current situation and control measures Evidence of natural recombination within the S1 gene of the infectious bronchitis virus Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses Sequence analysis of 1960s IBV isolates 503 Acknowledgements
- keywords: 1960s; analysis; avian; bronchitis; chickens; commercial; conn; conn46; countries; different; field; fig; gene; genetic; ibv; identity; infectious; isolates; l748; like; mass; mass41; mlv; number; phylogenetic; recombination; regions; related; rna; se17; sequence; serotypes; states; strains; type; type isolates; united; usa; vaccines; virus; viruses
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- cord-300104-855iw9wi
- author: Hennion, Ruth M.
- title: The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
- date: 2014-12-18
- words: 1490
- flesch: 71
- summary: The number of kidney cells obtained from each bird will vary with the age and the strain of the birds used. The technique for the production of kidney cell monolayer cultures from young birds, as described here, is adapted from those published by Dulbecco and Vogt [ 1 ] , and Youngner [ 2 ] for monkey kidney cells.
- keywords: ask; birds; blood; cells; chicken; cultures; edta; growth; ibv; kidney; monolayer; nbbs; note; old; pbsa; preparation; tissue; trypsin; viruses; volume
- versions: original; plain text
- cord-301720-majpfxqn
- author: Saadat, Yousef
- title: Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015
- date: 2017-09-15
- words: 2841
- flesch: 45
- summary: Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran Detection and identification of infectious bronchitis virusby RT-PCR in Iran Detection of the Italy 02 strain of infectious bronchitis virus in the UK Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods Molecular analysis of the 793/B serotype of IBV in Great Britain Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo Molecular studies on infectious bronchitis virus isolated in Poland Factors influencing the outcome of infectious bronchitis vaccination and challenge experiments Genotypes of infectious bronchitis viruses circulating in the Middle East between Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus indicates the emergence of a new genotype in the Middle East Genotyping of infectious bronchitis viruses from broiler farmsin Iraq during 2014-2015 Emergence of a novel genotype of avian infectious bronchitis virus in Egypt Al saad S. Presence of infectious bronchitis virus strain CK/CH/LDL/97I in the Middle East Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey Detection and molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012 Detection of variant infectious bronchitis viruses in broiler flocks in Libya Identification of a novel nephropathogenic infectious bronchitis virus in Israel Phylogenetic study of Iranian infectious bronchitis virus isolates during 2010 -2011 using glycoprotein S1 gene A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran Investigation and molecular characterization of avian infectious bronchitis virus in suspected broiler farms in Slemani Governorate The results emphasize the need for new control strategies and re-arrangement of preventative measure of IBV in Bushehr, Iran. One of a series of the Veterinary medicine-large animal clinical sciences department, Florida cooperative extension service Avian infectious bronchitis virus Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand Severe acute respiratory syndrome vaccine development experiences of vaccination against avian infectious bronchitis coronavirus Coronaviruses: Structure and genome expression Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus Structural characterization of the spike protein The coronavirus surface glycoprotein Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Identification of amino acids involved in a serotype and neutralization specific epitope within the S1 subunit of avian infectious bronchitis virus Structural polypeptides of coronavirus IBV Coronavirus proteins: Structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins Molecular Biology and Pathogenesis of Coronaviruses Monoclonal antibodies to the S1 spike and membrane proteins of avian infectious bronchitis virus coronavirus strain Massachusetts M41 Coronavirus IBV:
- keywords: acid; amino; avian; broiler; bronchitis; bushehr; characterization; chicken; coronavirus; disease; east; farms; field; flocks; gene; genotypes; glycoprotein; ibv; infectious; infectious bronchitis; iran; iranian; is/1494/06; isolates; middle; molecular; new; nucleotide; pcr; present; province; reaction; respiratory; samples; sequence; spike; strains; study; swabs; type; vaccination; vaccine; variant; virus; viruses
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- cord-301810-vtgdqart
- author: Aston, Emily J.
- title: Effect of Pullet Vaccination on Development and Longevity of Immunity
- date: 2019-02-02
- words: 7122
- flesch: 40
- summary: Criteria for evaluation of experimental infections Evaluation of the protection elicited by direct and indirect exposure to live attenuated infectious laryngotracheitis virus vaccines against a recent challenge strain from the United States Infectious bronchitis virus subpopulations in vaccinated chickens after challenge Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes The required sample size in vaccination-challenge experiments with infectious bronchitis virus, a meta-analysis Evaluating protection against infectious bronchitis virus by clinical signs, ciliostasis, challenge virus detection, and histopathology Virulence of infectious laryngotracheitis viruses: Comparison of modified-live vaccine viruses and North Carolina field isolates Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay Detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-PCR and correlation with virus detection in embryonated eggs Protection induced by infectious laryngotracheitis virus vaccines alone and combined with Newcastle disease virus and/or infectious bronchitis virus vaccines Optimization of a duplex real-time PCR method for relative quantitation of infectious laryngotracheitis virus Development and validation of a real-time Taqman PCR assay for the detection and quantitation of infectious laryngotracheitis virus in poultry Evaluation of infectious bronchitis virus Arkansas-type vaccine failure in commercial broilers Effect of vaccination on transmission characteristics of highly virulent Newcastle disease virus in experimentally infected chickens Latency and reactivation of infectious laryngotracheitis vaccine virus Effect of infection with a mesogenic strain of Newcastle disease virus on infection with highly pathogenic avian influenza virus in chickens Isolation of avian infectious bronchitis virus from experimentally infected chickens Histopathology of two serotypes of infectious bronchitis virus in laying Hens vaccinated in the rearing phase Comparative histopathology of two serotypes of infectious bronchitis virus (T and N1/88) in laying hens and cockerels Infectious bronchitis virus: Immunopathogenesis of infection in the chicken Lack of correlation between infectivity, serologic response and challenge results in immunization with an avian infectious bronchitis virus vaccine Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination Ambient ammonia does not inhibit the immune response to infectious bronchitis virus vaccination and protection from homologous challenge in broiler chickens Newcastle disease, other avian paramyxoviruses, and pneumovirus infections Comparative studies on the infectivity of avian respiratory viruses for eggs, cell cultures and tracheal explants Alternative methods to compare safety of live-attenuated respiratory Newcastle disease vaccines in young chicks Evaluation of the effect of live LaSota Newcastle disease virus vaccine as primary immunization on immune development in broilers Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody-based immunoperoxidase procedure Diseases of Poultry Cultivation of various avian viruses in pheasant trachea organ cultures and chick embryos Studies on a serum neutralization test for the diagnosis of laryngotracheitis in chickens The authors declare no conflict of interest. With the knowledge that live vaccine viruses can persist in flocks, it has been unclear, until now whether the immunity induced by a live vaccines could be compromised because of viral interference, a phenomenon in which one replicating virus blocks the infection and/or replication of another virus [9, 10] .
- keywords: age; attenuated; avian; birds; bronchitis; challenged; chickens; ciliostasis; clinical; collection; commercial; controls; days; detection; development; differences; different; disease; dna; dpc; figure; following; groups; higher; homologous; ibv; igg; iltv; immunity; infectious; inoculation; laryngotracheitis; lentogenic; live; loads; lower; ndv; negative; negative controls; newcastle; non; pathogens; pcr; positive; positive controls; post; poultry; protection; replication; respiratory; rna; scores; serum; significant; signs; specific; strain; study; table; times; titers; tracheal; usa; vaccinated; vaccinated birds; vaccinated controls; vaccination; vaccines; viral; virus; viruses; weeks; woa
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- cord-303393-9zs3qqo4
- author: Alsultan, Musaed Abdulaziz
- title: Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016
- date: 2019-03-19
- words: 4143
- flesch: 52
- summary: It is well known that there is a great diversity of S1 gene among IBV strains, which make it a strong candidate for the IBV classification [26] . In spite of the massive application of IBV vaccines in chicken farms under study, IBV is circulating, and thus, the currently used IBV vaccines under field conditions did not provide enough protection against the IBV infection.
- keywords: analysis; arabia; avian; birds; broiler; bronchitis; changes; chicken; control; death; different; ece; eggs; farms; gene; genotypes; hasa; high; ibv; ibv strains; infectious; infectious bronchitis; inoculated; isolation; kidneys; lungs; min; molecular; outbreaks; partial; passages; pcr; phylogenetic; positive; poultry; reaction; region; reported; respiratory; results; saudi; sequences; specimens; strains; study; taiwan; tissue; trachea; tree; vaccines; viral; virus
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- cord-303588-bwllypvq
- author: Ababneh, Mustafa
- title: High-resolution melting curve analysis for infectious bronchitis virus strain differentiation
- date: 2020-03-03
- words: 3150
- flesch: 54
- summary: Viral RNA of these samples and IBV vaccine strains were extracted, then went through cDNA synthesis, and then nested PCR amplification of a spike 1 gene fragment. IBV strains can be classified into six genotypes that together comprise 32 distinct viral lineages, the majority of which belong to genotype 1
- keywords: 4/91; able; analysis; assay; avian; bronchitis; chickens; cluster; curve; field; gene; high; hrm; ibv; infectious; isolates; jordan; like; mass; melting; min; nested; partial; pcr; qiagen; qrt; resolution; results; s1 gene; samples; sequence; sequencing; software; spike; strains; type; usa; vaccine; variant; virus
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- cord-303794-fn3jkiil
- author: Hassan, Kareem E.
- title: Experimental co-infection of infectious bronchitis and low pathogenic avian influenza H9N2 viruses in commercial broiler chickens
- date: 2017-06-30
- words: 3792
- flesch: 42
- summary: The shedding titers of AIV-H9N2 virus single infection decreased by 7 DPI from 6.2 to 4.5 log 10 , meanwhile in AIV-H9N2 with IBV mixed infection groups the shedding titer of AIV-H9N2 virus was significantly higher than the AIV-H9N2 single infection group. Remarkably, thymus of groups single AIV-H9N2 and AIV-H9N2 plus IBV was greatly affected with severe petechial hemorrhage followed by atrophy as compared to the negative control and single IBV infection groups.
- keywords: 4/91; aiv; antibody; avian; birds; blood; broiler; bronchitis; challenge; chickens; classical; clinical; commercial; congestion; control; day; disease; dpi; egypt; et al; experimental; fig; group; h9n2; h9n2n2; hassan; hemorrhage; high; ibv; infected; infection; influenza; like; mixed; mixed aiv; mortality; old; pathogens; pcr; poultry; respiratory; serum; severe; shedding; signs; single; strain; study; test; thymus; titers; tracheal; vaccine; variant; virus; viruses
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- cord-304278-0qy1nngs
- author: Raj, G. Dhinakar
- title: Infectious bronchitis virus: immunopathogenesis of infection in the chicken
- date: 2007-11-12
- words: 12541
- flesch: 33
- summary: Research in Veterinary Science Organ culture studies on the efficiency of infection of chicken tissues with avian infectious bronchitis virus Growth comparisons of avian infectious bronchitis virus strains in organ cultures of chicken tissues Taxonomic studies on strains of avian infectious bronchitis virus using neutralisation tests in tracheal organ cultures Changes in the Harderian gland of the chicken following conjunctival and intranasal infection with infectious bronchitis virus in one and 20 day-old chickens Influence of maternal antibodies on vaccination of chicks of different ages against infectious bronchitis virus Effect of removal of the Harderian gland in one day-old chicks on immunity following infectious bronchitis vaccination Study on the local effect of eye-drop vaccination against infectious bronchitis in one day-old chicks with maternal antibodies A study on the synthesis and secretion of immunoglobulins by the Harderian gland of fowl after eye-drop vaccination against infectious bronchitis at 1 day-old Comparison of the enzyme linked immunosorbent assay, the haemagglutination-inhibition test and agar gel precipitation test for the detection of antibody against infectious bronchitis and Newcastle disease in commercial broilers Immunopathogenesis of infection in SPF chicks and commercial broilers of a variant infectious bronchitis virus of economic importance Local antibody production in the oviduct and gut of hens infected with an enterotropic strain of infectious bronchitis virus An in-vitro comparison of the virulence of seven strains of infectious bronchitis virus using tracheal and oviduct organ cultures Protectotypic differentiation of avian infectious bronchitis viruses using an in-vitro challenge model Effect of T-cell suppression by cyclosporin on primary and persistent infections of infectious bronchitis virus in chickens Cross-reactive cellular immune responses in chickens vaccinated with live infectious bronchitis vaccine Effect of heterophil depletion by 5-fluorouracil on infectious bronchitis virus infection in chickens Swollen head syndrome associated with Escherichia coli and infectious bronchitis virus in the Central Valley of California The isolation and characterisation of six avian infectious bronchitis virus isolated in Morocco Experimental production of complicated chronic respiratory disease (air-sac disease) Cellular response of the respiratory tract of chickens to infection with Massachusetts 41 and Australian T infectious bronchitis viruses Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens Attenuation of avian infectious bronchitis virus by cold adaptation Effects of infectious bursal disease on the responsiveness of the chickens to Mycoplasma synoviae, Newcastle disease and infectious bronchitis Avian infectious bronchitis: demonstration of serum IgG and IgM neutralising antibody by sucrose density gradient centrifugation and mercaptoethanol reduction Local antibody responses to avian infectious bronchitis: virus neutralising antibody in tracheobronchial secretions Local immunity to avian infectious bronchitis virus in tracheal organ cultures Relationship of common avian pathogen antibody-titres in so-called chicken anaemia agent (CAA)-antibodypositive chicks to titers in CAA-antibody-negative chicks Comparison of serological tests for the measurement of primary immune response to avian infectious bronchitis virus vaccines A new strain of infectious bronchitis virus infecting domestic fowl in Great Britain The role of Escherichia coli in the cause of chronic respiratory disease and certain other poultry diseases Strategy of protection of the layer against infectious bronchitis virus Presence of viral antigens and antibody in the trachea of chickens infected with avian infectious bronchitis virus Chemical pathology of nephrosis induced by infectious bronchitis virus Infectious bronchitis virus strain differentiation attempts by cross-immunity studies in tracheal organ cultures derived from immunised chickens Evaluation of the immune response to infectious bronchitis virus Induction of chicken interferon by avian infectious bronchitis virus. Interferon induction by and sensitivity to interferon of IBV Interferon induction by several strains of avian infectious bronchitis virus, a corona virus, in chickens Comparison of the susceptibility to avian infectious bronchitis virus infection of two inbred lines of white leghorn chickens Interferon induction by avian infectious bronchitis virus Detection of viral antigen following exposure of one-day old chickens to the Holland-52 strain of infectious bronchitis virus Characterisation of an infectious bronchitis virus isolated from vaccinated broiler breeder flocks Electron microscope observations on the entry of avian infectious bronchitis virus into susceptible cells The effect of live infectious bronchitis vaccine on the course of infectious laryngotracheitis The susceptibility of chicken kidney and oviduct organ cultures to a vaccine strain of infectious bronchitis virus )
- keywords: affected; age; ambali; antibodies; antibody; antigenic; assay; australian; avian; avian infectious; birds; broiler; bronchitis virus; cause; cavanagh; cells; cellular; challenge; changes; chickens; chicks; chronic; clear; clinical; coli; comparison; cook; coronavirus; correlation; crinion; cross; csa; cultures; cumming; damage; darbyshire; davelaar; days; des; detection; dhinakar; differences; different; disease; effect; egg; elisa; enterotropic; epithelial; escherichia; et al; evidence; excretion; experimental; factors; field; fowl; gland; glycoprotein; greater; gut; harderian; head; hens; high; higher; humoral; ibv; ibv infection; ibv strains; iga; igg; immune; immunity; immunological; important; increase; induced; induction; infected; infection; infectious bronchitis; inoculation; isolates; isolation; jones; kidneys; lachrymal; lesions; levels; lines; live; local; lymphocytes; m41; massachusetts; mockett; model; mortality; mycoplasma; nakamura; nephritis; nephrosis; new; nibv; old; organ; otsuki; oviduct; p.i; pathogenesis; pathogenicity; persistence; presence; present; protection; protein; raj; recovery; reduction; replication; research; resistant; respiratory; response; role; sensitive; serotypes; serum; severe; severity; signs; similar; site; specific; spike; strains; studies; study; susceptibility; susceptible; swollen; syndrome; tests; tissues; titres; toro; tracheal; tract; tubular; und; vaccinated; vaccination; vaccines; variant; viral; virulence; virus; viruses; vitro; weeks
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- cord-304322-k9egxskw
- author: Promkuntod, N.
- title: Analysis of the S1 gene of the avian infectious bronchitis virus (IBV) reveals changes in the IBV genetic groups circulating in southern Thailand
- date: 2015-06-30
- words: 2255
- flesch: 43
- summary: Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction Avian infectious bronchitis in the southern part of Thailand S1 glycoprotein gene analysis of avian infectious bronchitis virus strains isolated in southern Thailand Viral potentiation of bacterial superinfection of the respiratory tract Coronavirus avian infectious bronchitis virus Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys Studies on the attenuated on infectious bronchitis virus The long view: 40 years of infectious bronchitis research Infectious bronchitis virus variants: a review of the history, current situation and control measures Isolation and propagation of coronaviruses in embryonated eggs Cross-immunity in chickens using seven isolates of avian infectious bronchitis virus The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens Molecular evolution and emergence of avian gammacoronaviruses Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009: identification of natural recombination in the field isolates Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins MEGA6: molecular evolutionary genetics analysis version 6.0 Molecular epidemiology of infectious bronchitis virus isolates from China and Southeast Asia Isolation and identification of glandular stomach type IBV (QX IBV) in chickens Geographic distribution of field isolates of IBV in southern Thailand between This work was supported by Department of Livestock Development, Ministry of Agriculture and Cooperatives, Thailand (Project 85/2013).
- keywords: acid; amino; analysis; available; avian; birds; bronchitis; chickens; commercial; fig; gene; glycoprotein; group; ibv; infectious; isolates; like; nucleotide; phylogenetic; poultry; predominant; range; respiratory; rna; sequences; single; southern; strains; symptoms; tha001; thailand; type; vaccine; variants; viral; virus; viruses
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- cord-305079-foifc8ch
- author: Zhou, Ying Shun
- title: Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120
- date: 2013-02-22
- words: 4865
- flesch: 44
- summary: The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity A recombinant avian infectious bronchitis virus expressing a heterologous spike gene belonging to the 4/91 serotype Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: a review Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot Genes 3 and 5 of infectious bronchitis virus are accessory protein genes Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Reverse genetics system for the avian coronavirus infectious bronchitis virus Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides Nidovirales: a new order comprising Coronaviridae and Arteriviridae Coronavirus avian infectious bronchitis virus Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions Newcastle disease virus, a host range-restricted virus, as a vaccine vector for intranasal immunization against emerging pathogens An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells The pathogenesis of virulent and avirulent avian infectious bronchitis virus Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication Eightplasmid system for rapid generation of influenza virus vaccines A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse genetics A new influenza virus virulence determinant: the NS1 protein four Cterminal residues modulate pathogenicity Effect of IBV-H120 vaccination in broilers on colibacillosis susceptibility after infection with a virulent Massachusetts-type IBV strain Recombinant Newcastle disease virus as a vaccine vector Manual of Diagnostic Tests and Vaccines for Terrestrial Animals A simple method of estimating fifty per cent endpoints Coronaviruses of domestic livestock and poultry: interspecies transmission, pathogenesis, and immunity The Springer Index of Viruses Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenza In vitro assembled, recombinant infectious bronchitis viruses demonstrate that the 5a open reading frame is not essential for replication Strategy for systematic assembly of large RNA and DNA genomes: transmissible gastroenteritis virus model Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59 Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120 The remaining one third of the genome encodes the structural proteins and group-specific ORFs, including spike glycoprotein (S), envelope protein (E), membrane Veterinary Microbiology 162 (2013) Infectious bronchitis virus (IBV) strain H120 was successfully rescued as infectious clone by reverse genetics.
- keywords: agarose; allantoic; assembly; avian; bhk-21; biological; bronchitis; casais; casais et; cdna; cell; characteristics; chickens; clone; control; coronavirus; day; development; ece; eggs; eid50; et al; fig; fluid; fragments; genetics; genome; group; h120; ibv; immunized; infectious; length; old; parental; pbs; pcr; post; present; replication; reverse; rna; similar; spf; strain; study; system; titer; transcription; vaccine; vector; viral; virus; vitro; youn
- versions: original; plain text
- cord-305684-ipeup5mp
- author: Chen, Yuqiu
- title: Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China
- date: 2016-12-16
- words: 5167
- flesch: 45
- summary: Presence of infectious bronchitis virus strain CK/CH/LDL/97I in the middle east Early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus Recent studies on the enterotropic strain of avian infectious bronchitis virus Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks Comparison of the pathogenicity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions Coronavirus avian infectious bronchitis virus Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China Coronaviruses: important emerging human pathogens Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination Infection of day old chicks with infectious bronchitis (IB) virus and subsequent anatomical abnormalities Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China Altered pathogenicity of a tl/CH/LDT3/03 genotype infectious bronchitis coronavirus due to natural recombination in the 5'-17 kb region of the genome Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus Susceptibility of three genetic lines of chicks to infection with a nephropathogenic T strain of avian infectious bronchitis virus Emergence of a group 3 coronavirus through recombination Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China Comparative analysis of four Massachusetts type infectious bronchitis coronavirus genomes reveals a novel Massachusetts type strain and evidence of natural recombination in the genome Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Phylogenetic analysis of the S1 glycoprotein gene of infectious bronchitis viruses isolated in China during Phylodynamic analysis of avian infectious bronchitis virus in South America The molecular biology of coronaviruses Identification of a novel coronavirus from a beluga whale by using a panviral microarray Attempts to reproduce a runting/stunting-type syndrome using infectious agents isolated from affected Mississippi broilers A novel variant of the infectious bronchitis virus resulting from recombination events in Italy and Spain Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins A simple method of estimating fifty percent endpoints Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification The avian coronavirus spike protein Discovery of a novel bottlenose dolphin coronavirus reveals a distinct species of marine mammal coronavirus in Gammacoronavirus Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene The CH III and A2 genotypes have previously been used to classify other IBV genotypes in China (Liu et al., 2006; Xu et al., 2007) .
- keywords: acid; allantoic; amino; analysis; avian; birds; bronchitis; chickens; chicks; china; complete; control; coronavirus; days; dead; different; distinct; dpc; et al; fig; flocks; gene; genome; genotype; gi-28; group; ibv; ibvs; identities; infectious; kidneys; ldl/97i; lesions; lgx/111119; like; lx4; massachusetts; molecular; neutralization; new; novel; nucleotide; old; oviducts; phylogenetic; protein; recombination; reference; regions; respiratory; results; rna; sequence; serotype; strain; strain ck; study; viral; virus; viruses
- versions: original; plain text
- cord-306380-msk9p1yy
- author: Lee, C.-W.
- title: Evidence of genetic diversity generated by recombination among avian coronavirus IBV
- date: 2000
- words: 2800
- flesch: 54
- summary: Sequencing primers to various regions of the gene for DE072 and The relative primer positions were calculated using the ATG start site of Gene 3 as 1 for primers gene 3 and 4, and ATG start site of S1 gene as 1 for primers HVR in S1 D1466 were designed using OLIGO version 4.0 software (National Bioscience, Plymouth, MN, USA) and are available upon request. Gene 4 consisted of the M protein gene with a single ORF and a non-coding region between the 3 end of the M protein gene and gene 5.
- keywords: analysis; avian; bronchitis; coding; d1466; de072; different; end; field; fig; gene; genome; holl52; hvr; ibv; infectious; isolates; non; nucleotide; pcr; phylogenetic; polymerase; protein; recombination; region; rna; sequence; serotype; similarity; site; strains; usa; viral; virus
- versions: original; plain text
- cord-306976-p2521bl4
- author: Gao, Mengying
- title: Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China
- date: 2016-08-15
- words: 5125
- flesch: 48
- summary: Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks Comparison of the pathogenicity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions Effects of infectious bronchitis on egg production Effects of infectious bronchitis in baby chicks Infectious bronchitis Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China Pathogenicity of four serotypes of avian infectious bronchitis virus for the oviduct of young chickens of varying ages Egg quality and production following infectious bronchitis virus exposure at one day old Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination Infection of day old chicks with infectious bronchitis (IB) virus and subsequent anatomical abnormalities A comparative study of pigeons and chickens experimentally infected with PPMV-1 to determine antigenic relationships between PPMV-1 and NDV strains A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus Persistence of virus in the tissues and development of the oviduct in the fowl following infection at day old with infectious bronchitis virus Altered pathogenicity, immunogenicity, tissue tropism and 3 0 -7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos Evaluation of the protection conferred by commercial vaccines and attenuated heterologous isolates in China against the ck/CH/LDL/97I strain of infectious bronchitis coronavirus Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3 0 untranslated region Identification of a novel nephropathogenic infectious bronchitis virus in Israel A simple method of estimating fifty percent endpoints MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination key: cord-306976-p2521bl4 authors: Gao, Mengying; Wang, Qiuling; Zhao, Wenjun; Chen, Yuqiu; Zhang, Tingting; Han, Zongxi; Xu, Qianqian; Kong, Xiangang; Liu, Shengwang title: Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China date: 2016-08-15 journal: Vet Microbiol DOI: 10.1016/j.vetmic.2016.05.018 sha: doc_id: 306976 cord_uid: p2521bl4 Since 2009, strains of the naturally recombinant TW I genotype of infectious bronchitis virus (IBV) have caused considerable damage to the Chinese poultry industry.
- keywords: addition; age; attenuated; birds; bronchitis; challenge; chickens; china; conn; control; coronavirus; cystic; day; different; dpc; et al; gene; genotype; groups; ibv; infectious; ldl/091022; ldl/97i; ldt3; lgx/100508; like; lsd/120720; lx4; negative; nrtw; old; oviducts; p120; p70; p90; protection; results; serotype; spf; strain; study; table; type; vaccinated; vaccination; vaccines; virus; viruses; wit
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- cord-307304-irji8owi
- author: Britton, Paul
- title: Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection
- date: 2004-11-05
- words: 4883
- flesch: 37
- summary: Molecular analysis of the structure and the role of individual genes in pathogenesis of RNA viruses has been advanced by the availability of full-length cDNAs, for the generation of infectious RNA transcripts that can replicate and result in infectious viruses from permissive cell lines (Boyer and Haenni, 1994) . Transfection of the BAC DNA into susceptible cells resulted in synthesis of infectious RNA and the recovery of infectious virus (Almazán et al., 2000) .
- keywords: analysis; assembly; avian; bamhi; beau; beaudette; bronchitis; casais; casais et; cavanagh; cdna; cells; chimaeric; ck cells; ck s; coronavirus; ecogpt; ectodomain; et al; fig; gene; gene sequence; genome; growth; homologous; ibv; ibv cdna; infected; infectious; length; length ibv; m41s; medium; method; mpa; pcr; pgpt; plasmid; polymerase; protein; recombinant; recombination; ribv; rna; s gene; selection; sequence; spike; system; tds; tdsr; vaccinia; vaccinia virus; vero; virus; viruses; vnoti
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- cord-308591-cs8os2f5
- author: Tawakol, Maram M.
- title: Evaluation of bacteriophage efficacy in reducing the impact of single and mixed infections with Escherichia coli and infectious bronchitis in chickens
- date: 2019-11-29
- words: 4184
- flesch: 41
- summary: Complications between APEC and IBV infections are widely observed under field conditions and are reproduced under experimental conditions resulting in enhanced severe colibacillosis [7] and enhanced pathogenicity post IBV infection. Regarding IBV infection, clinical signs were observed from four-days postchallenge, with bacteriophage treatment leading to a reduction of their severity, but not delaying their onset (Figure 3) .
- keywords: accordance; apec; avian; bacterial; bacteriophage; birds; broiler; bronchitis; characterization; chickens; clinical; coli; colibacillosis; collected; conditions; days; disease; e.coli; egypt; farms; figure; flocks; genotype; groups; high; higher; ibv; infected; infections; isolated; isolation; losses; mixed; molecular; mortality; non; o78; pathogenicity; pathogens; positive; poultry; presence; present; reduction; respiratory; samples; severe; shedding; significant; signs; single; spike; strains; study; time; tract; treatment; variant; viral; virus; viruses
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- cord-308950-bl83r4v3
- author: Miguel, B.
- title: The role of feline aminopeptidase N as a receptor for infectious bronchitis virus : Brief Review
- date: 2002
- words: 2550
- flesch: 44
- summary: To determine if IBV could replicate in the FEK cells, cell cultures were infected with Ark/IBV and the supernatants from infected cells were collected at 1, 2, 3 and 10 d PI. The objectives of this study were to: (1) determine if feline cells were permissive to Arkansas 99 (Ark 99) serotype of IBV, (2) evaluate if the IBV can replicate in feline cells, and (3) determine the contribution of APN to permissiveness of feline cells.
- keywords: 229e; aminopeptidase; ark; bronchitis; canine; cats; cd13; cells; chicken; cmv; control; coronavirus; culture; determine; different; disease; eid; epithelial; evidence; experimental; expression; fapn; fek; feline; glycoprotein; group; human; ibv; infected; infectious; pbk; pbs; permissive; receptor; recombination; region; role; species; surface; virus
- versions: original; plain text
- cord-309469-2naxn580
- author: An, Hongliu
- title: Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
- date: 2019-01-05
- words: 3609
- flesch: 40
- summary: Noncoding RNAs of plant viruses and viroids: sponges of host translation and RNA interference machinery Noncoding RNAs of plant viruses and viroids: sponges of host translation and RNA interference machinery A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability A highly structured, nuclease-resistant, noncoding RNA produced by flaviviruses is required for pathogenicity RNA of low molecular weight in KB cells infected with adenovirus type 2 A contemporary view of coronavirus transcription Noncoding flavivirus RNA displays RNA interference suppressor activity in insect and Mammalian cells West Nile virus noncoding subgenomic RNA contributes to viral evasion of the type I interferon-mediated antiviral response Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development EBV noncoding RNAs Role of nucleotides immediately flanking the transcription-regulating sequence core in coronavirus subgenomic mRNA synthesis Noncoding RNPs of viral origin Decoding human cytomegalovirus Polyadenylylated nuclear RNA encoded by Kaposi sarcoma-associated herpesvirus Parvovirus expresses a small noncoding RNA that plays an essential role in virus replication Dissection of the adenoviral VA RNAI central domain structure reveals minimum requirements for RNA mediated inhibition of PKR Identification of a noncanonical signal for transcription of a novel subgenomic mRNA of mouse hepatitis virus: implication for the mechanism of coronavirus RNA transcription Characterization of ribonucleoprotein complexes containing an abundant polyadenylated nuclear RNA encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) Sequence motifs involved in the regulation of discontinuous coronavirus subgenomic RNA synthesis This work was financially supported by grants from the National Natural Science Foundation of China (No. 31572490) and the Department of Science and Technology, Hubei Provincial People's Government, China (No. 2013BHE020) For example, subgenomic RNA (sfRNA) produced by Flaviviruses, such as Dengue virus (DENV), West Nile virus (WNV), Yellow fever virus (YFV), and Zika virus [reviewed in (Bidet and Garcia-Blanco, 2014; Roby et al., 2014; Pijlman et al., 2008; Akiyama et al., 2016) , and subgenomic ncRNA generated by some plant viruses such as Barley yellow dwarf virus and Red clover necrotic mosaic virus using similar mechanism (Miller et al., 2016a) .
- keywords: 3′-utr; bronchitis; c27107; cells; cellular; chicken; core; coronavirus; discontinuous; dmem; encoding; end; et al; expression; fbs; fig; gene; generation; genome; herpesvirus; host; human; ibv; infected; infectious; leader; manufacturer; mechanism; membrane; mouse; mrna; ncrna; noncanonical; noncoding; novel; nucleotides; pcr; plaque; protein; reverse; ribv; rna; rnas; sequence; sgrna; subgenomic; synthesis; time; total; transcription; translation; trs; uaaca; vero; viral; virus; viruses
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- cord-309623-2ngr682l
- author: Han, Xiaoxiao
- title: Infectious Bronchitis Virus Infection Induces Apoptosis during Replication in Chicken Macrophage HD11 Cells
- date: 2017-07-26
- words: 5630
- flesch: 43
- summary: Cell apoptosis induced by virus may cause tissue damage, especially in the immune and nervous systems, suggesting that apoptosis is a pathogenic mechanism in virus-induced disease. The molecular and morphological variations in IBV-infected cells revealed that cell apoptosis was induced by IBV infection and appeared to activate caspase-8 by the Fas/Fas ligand (FasL)-mediated signaling pathway and to activate caspase-9 by the B-cell lymphoma 2 (Bcl-2) family-mediated signaling pathway.
- keywords: activation; activity; apoptosis; apoptotic; assay; avian; bax; beaudette; biotech; bronchitis; caspase-8; cells; chicken; china; coronavirus; cultured; data; dependent; expression; extrinsic; family; fas; fasl; figure; fmk; h.p.i; hd11; hd11 cells; ibv; ibv beaudette; immune; important; induction; infected; infection; inhibitor; intrinsic; jiangsu; levels; macrophage; moi; pathway; pcr; protein; province; rate; replication; results; role; specific; strain; studies; study; test; time; titer; treated; viral; virus; viruses
- versions: original; plain text
- cord-310372-qc6941pm
- author: Ji, Jun
- title: Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009
- date: 2011-04-22
- words: 3576
- flesch: 42
- summary: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or hemagglutination-inhibiting antibody, or induce chicken tracheal protection Relationship between variation in the S1 spike protein of infectious bronchitis virus and the extent of crossprotection Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Antigenic and S-1 genomic characterization of the Delaware variant serotype of infectious bronchitis virus Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Analysis of the serotypespecific epitopes of avian infectious bronchitis virus strains Ark99 and Mass41 Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses Different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in China Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene Current status of infectious bronchitis virus infections in chickens and prospects for control by vaccines Maternal antibody to infectious bronichitis virus: its role in protection against infection and development of active immunity to vaccine A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and nonvaccinated flocks in China Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus Co-circulation of four types of infectious bronchitis virus (793/B, 624/I, B1648 and Massachusetts) Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B) A nomenclature for avian coronavirus isolates and the question of species status Infectious bronchitis Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus The experimental infection of chickens with mixtures of infectious bronchitis virus and Escherichia coli Significance of interactions between Escherichia coli and respiratory pathogens in layer hen flocks suffering from colibacillosisassociated mortality Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus The molecular biology of coronaviruses Coronavirus IBV: removal of spike glycopolypeptide S1 by urea abolishes infectivity and haemagglutination but not attachment to cells Location of the amino-acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Coronavirus IBV:
- keywords: acid; amino; analysis; avian; birds; branch; bronchitis; bronchitis virus; chickens; china; clinical; control; coronavirus; days; deletions; different; disease; field; flocks; gene; glycoprotein; gray; hn08; ibv; ibv strains; ibvs; infectious; infectious bronchitis; insertions; isolated; like; m41; nephropathogenic; new; nucleotide; outbreaks; pcr; phylogenetic; protection; protein; province; reference; respiratory; results; sequence; spike; strains; study; table; type; vaccine; virus
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- cord-310536-u30cufg7
- author: Finger, Paula Fonseca
- title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis
- date: 2018-12-12
- words: 4667
- flesch: 47
- summary: key: cord-310536-u30cufg7 authors: Finger, Paula Fonseca; Pepe, Michele Soares; Dummer, Luana Alves; Magalhães, Carolina Georg; de Castro, Clarissa Caetano; de Oliveira Hübner, Silvia; Leite, Fábio Pereira Leivas; Ritterbusch, Giseli Aparecida; Esteves, Paulo Augusto; Conceição, Fabricio Rochedo title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis date: 2018-12-12 journal: Virol J DOI: 10.1186/s12985-018-1096-2 sha: doc_id: 310536 cord_uid: u30cufg7 BACKGROUND: The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. In order to evaluate the repeatability of the ELISA-rN, three separate batches of recombinant N protein were produced and purified following the methodology described above with distinct purification times, to demonstrate the reproducibility of the recombinant protein expression procedure.
- keywords: antibodies; antibody; antigen; avian; bands; blot; bronchitis; bronchitis virus; chromatography; commercial; commercial elisa; commercial test; detection; developed; development; diagnosis; disease; elisa; expression; graphpad; ibv; idexx; infectious; infectious bronchitis; kda; kit; min; negative; nucleocapsid; page; pbs; pcr; plates; positive; protein; recombinant; results; rn protein; rn test; samples; sensitivity; sera; serum; sigma; software; specificity; study; techniques; test; usa; use; virus; western; western blot
- versions: original; plain text
- cord-312489-ywep0c08
- author: Andoh, Kiyohiko
- title: Decreased neutralizing antigenicity in IBV S1 protein expressed from mammalian cells
- date: 2015-10-02
- words: 5052
- flesch: 35
- summary: These results indicated that while recombinant S1 protein retained antigenicity (the ability to induce antibodies against S1 protein), the resulting antibodies was decreased its neutralizing activity, in contrast to those induced by inactivated virus. Results showed that the recombinant S1 protein was highly glycosylated, and the neutralizing antigenicity of recombinant S1 protein was lower than that of inactivated virus.
- keywords: ability; antibodies; antibody; antigen; antigenicity; avian; band; blotting; bronchitis; buffer; cavanagh; cells; chickens; conformation; control; coronavirus; domain; elisa; encoding; epitopes; et al; expression; fig; gene; glycoprotein; glycosylation; host; ibv; immunization; immunized; important; inactivated; induced; infectious; mammalian; mixed; motif; native; neutralizing; pbs; pcaggs; peptide; protection; protein; purified; recombinant; recombinant s1; region; results; s1 protein; sequence; sera; spike; strain; tm86; trimerization; vaccine; virus; western
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- cord-313676-6rebpe57
- author: De la Torre, David I.
- title: Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks
- date: 2018-03-29
- words: 5876
- flesch: 44
- summary: Detection of rotaviruses and intestinal lesions in broiler chicks from flocks with runting and stunting syndrome (RSS) Investigation into the aetiology of runting and stunting syndrome in chickens A metagenomic comparison of endemic viruses from broiler chickens with runting stunting syndrome and from normal birds Detection of enteric viruses in pancreas and spleen of broilers with runting-stunting syndrome A retrospective study on poult enteritis syndrome in Minnesota Epidemiological study of enteric viruses in broiler chickens: Comparison of tissue culture and direct electron microscopy Simultaneous detection of astrovirus, rotavirus, reovirus and adenovirus type I in broiler chicken flocks Enteric viruses in Brazilian turkey flocks: turkeys A Multiplex RT-PCR test for the differential identification of turkey astrovirus type 1, turkey astrovirus type 2, chicken astrovirus, avian nephritis virus, and avian rotavirus Enteric viruses detected by molecular methods in commercial chicken and turkey flocks in the United States between Partial genome sequence analysis of parvoviruses associated with enteric disease in poultry Isolation of high-molecular-weight DNA using organic solvents Single-step method of RNA isolation by acid guanidinium extraction Development of a polymerase chain reaction procedure for detection of chicken and turkey parvoviruses Detection and molecular characterization of chicken astrovirus associated with chicks that have an unusual condition known as white chicks in Brazil The neighbor-joining method: A new method for reconstructing phylogenetic trees Confidence limits on phylogenies: An approach using the bootstrap Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees Molecular survey of enteric viruses in commercial chicken farms in Korea with a history of enteritis Chicken parvovirus-Induced runting-stunting syndrome in young broilers Detection and molecular characterization of enteric viruses in breeder turkeys Naturally occurring parvoviral infection in Hungarian broiler flocks Molecular characterization of avian astroviruses Chicken parvovirus viral loads in cloacal swabs from malabsorption syndrome-affected and healthy broilers Molecular characterization and typing of chicken and turkey astroviruses circulating in the United States: Implications for diagnostics Epidemiological investigation of outbreaks of fowl adenovirus infections in commercial chickens in China Chicken anemia virus and fowl adenoviruses: Association to induce the inclusion body hepatitis/hydropericardium syndrome Viral agents related to enteric disease in commercial chicken flocks, with special reference to Latin America Multisystemic adenovirus infection in broiler chicks with hypoglycemia and spiking mortality Characterization of fowl adenoviruses isolated in Ontario and Quebec Detection and quantitation of fowl adenovirus genome by a real-time PCR assay Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses Lesions of the Avian Pancreas Parvovirus-associated cerebellar hypoplasia and hydrocephalus in day old broiler chickens Molecular detection of chicken parvovirus in broilers with enteric disorders presenting curving of duodenal loop, pancreatic atrophy, and mesenteritis Sequence analyses of the representative chinese-prevalent strain of avian nephritis virus in healthy chicken flocks Sciences, A. Detection and partial genetic characterisation of a novel variant of avian nephritis virus in Indian poultry flocks showing diverse clinical signs High-resolution melt curve analysis to confirm the presence of co-circulating isolates of avian nephritis virus in commercial chicken flocks Comparative dynamic distribution of avian infectious bronchitis virus M41, H120, and SAIBK strains by quantitative real-time RT-PCR in SPF chickens Comparative analysis of the intestinal bacterial and RNA viral communities from sentinel birds placed on selected broiler chicken farms A primary epidemic of inclusion body hepatitis in broilers Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile Identification and virulence characterization of fowl adenoviruses in the Republic of Korea Inclusion body hepatitis associated with an outbreak of fowl adenovirus type 2 and type 8b in broiler flocks in South Africa Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland Genetic characterization of parvoviruses circulating in turkey and chicken flocks in Poland Genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences Detection of avian nephritis virus in Australian chicken flocks Enteric viruses in turkey enteritis Genome sequences of rotavirus A strains Ty-1 and Ty-3, isolated from turkeys in Ireland in 1979 Molecular epidemiology of avian rotaviruses group A and D shed by different bird species in Nigeria
- keywords: adenovirus; age; analysis; anv; areo; artv; astrovirus; avian; birds; brazilian; breeders; broilers; castv; cdna; characterization; chicken; chpv; clinical; commercial; common; detection; different; distribution; dna; enteric; fadv; flocks; fowl; group; ibv; infections; isolates; layers; min; molecular; multiple; parvovirus; pcr; phylogenetic; polymerase; positive; poultry; production; reaction; reference; results; rotavirus; samples; sequences; signs; single; states; study; stunting; syndrome; table; turkey; type; viral; viruses
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- cord-316153-wet0go35
- author: Jia, W.
- title: A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains
- date: 1995
- words: 3937
- flesch: 53
- summary: Phagemids containing IBV genes were prepared by in vivo excision in Escherichia coli (E. coli) using a protocol furnished by Stratagene (La Jolla, CA). In both the indirect and antigen-capture ELISA, the Ark-specific and Mass-specific MAbs reacted with both the whole virions and the purified S1 protein of CU T2 strain (Table 1) .
- keywords: acid; amino; antigenic; ark99; avian; bases; bronchitis; cdna; chicken; comparison; coronavirus; data; different; end; epitopes; evidence; field; fig; fragment; gene; generation; genetic; genomic; glycoprotein; ho1152; homology; ibv; infectious; isolates; kb8523; mabs; mass; mass41; mutation(s; nature; non; protein; recombination; region; rna; sequence; sequencing; serotype; specific; spike; strains; t t; vaccine; variants; virus; viruses
- versions: original; plain text
- cord-316234-vtjsfi2c
- author: Sultankulova, Kulyaisan T.
- title: New oligonucleotide microarray for rapid diagnosis of avian viral diseases
- date: 2017-04-05
- words: 4700
- flesch: 40
- summary: Re: scientific interest in Newcastle disease virus is reviving Molecular and structural bases for the antigenicity of VP2 of infectious bursal disease virus Laboratory manual for isolation and identification of avian pathogens Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection Coronavirus avian infectious bronchitis virus Review of rapid diagnostic tests for influenza Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1 A multiplex reverse transcription-polymerase chain reaction assay for Newcastle disease virus and avian pneumovirus (Colorado strain) Avian influenza virus detection and quantitation by real-time RT-PCR Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis Development of a real-time TaqMan RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation Real-time RT-PCR differentiation and quantitation of infectious bursal disease virus strains using dual-labeled fluorescent probes Standard epidemiological methods to understand and improve Apis mellifera health Avian influenza: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses Rapid PCR-based molecular pathotyping of H5 and H7 avian influenza viruses Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus Development of a multiplex real-time polymerase chain reaction for the detection of influenza virus type A including H5 and H9 subtypes Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers Molecular characterization of seven Chinese isolates of infectious bursal disease virus: classical, very virulent and variant strains Infectious bursal disease viruses: molecular differentiation of antigenic subtypes among serotype I viruses Molecular identification of infectious bursal disease virus strains Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay Phylogeny of antigenic variants of Avian Coronavirus IBV
- keywords: agent; aiv; analysis; assay; avian; birds; bronchitis; bursal; data; dead; detection; diagnosis; different; differentiation; disease; dna; epidemiological; fragments; gene; hybridization; ibdv; ibv; infectious; influenza; isolation; methods; microarray; min; molecular; multiplex; ndv; newcastle; oligonucleotide; pcr; positive; poultry; primers; probes; rapid; reaction; real; results; samples; sensitivity; sequences; simultaneous; specificity; specimens; strains; study; test; testing; time; time rt; usa; use; viral; virus; viruses; vp2
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- cord-316525-uadfehr6
- author: Zhang, X. W.
- title: Testing the hypothesis of a recombinant origin of the SARS-associated coronavirus
- date: 2004-10-11
- words: 3026
- flesch: 41
- summary: The mutation analysis of sequence variations among these isolates will help identify the genetic signature of SARS virus strains when a sufficient amount of sequence data is available. The left panels stand for non-recombination regions and the right panels for recombination regions.
- keywords: alignment; analysis; animals; bcov; breakpoints; bronchitis; coronaviruses; cov; detection; different; events; evolution; fig; geneconv; genome; hcov; hmm; human; ibv; infectious; like; methods; mhv; mosaic; origin; pedv; phylogenetic; potential; present; protein; putative; rdp; recombination; regions; replicase; rna; sars; sequence; study; table; tgev
- versions: original; plain text
- cord-317347-by8albr9
- author: van Ginkel, Frederik W.
- title: Age-dependent immune responses and immune protection after avian coronavirus vaccination
- date: 2015-05-28
- words: 5815
- flesch: 50
- summary: These data strongly support IBV vaccination after day 7 post hatch. To determine whether age of IBV vaccination affected the humoral immune response, plasma samples were collected 14 and 21 days and tears 14 days after vaccination with 3 × 10 5 EID 50 of a live-attenuated ArkDPI IBV vaccine strain on 1, 7 14, 21 or 28 days of age.
- keywords: age; age group; antibodies; antibody; avian; avidity; birds; bronchitis; cells; challenge; chickens; chicks; control; data; day; days; delay; dependent; different; early; elisa; field; fig; goblet; group; higher; ibv; iga; igg; igm; immune; immunity; index; industry; infectious; lack; levels; life; lower; lymphoid; maternal; mean; mucosal; natural; old; older; plasma; post; poultry; protection; respiratory; response; samples; significant; specific; spf; strain; supplemental; system; tears; test; titers; tracheal; vaccinated; vaccination; vaccine; virus; week
- versions: original; plain text
- cord-317587-rrx2r4n2
- author: Fan, Wensheng
- title: Genetic Analysis of Avian Coronavirus Infectious Bronchitis Virus in Yellow Chickens in Southern China over the Past Decade: Revealing the Changes of Genetic Diversity, Dominant Genotypes, and Selection Pressure
- date: 2019-09-26
- words: 9303
- flesch: 52
- summary: The mean substitution rates for the S1, E, M, and N genes of the epidemic isolates during 1985-2017 were calculated to be 4.6 × 10 −3 , 4.3 × 10 −3 , 3.9 × 10 −3 , and 3.7 × 10 −3 substitutions/site/year (s/s/y), respectively ( Table 5 ), indicating that S1 gene is the most easily mutated and N gene is the most stable among the four structural genes. It is known that S1 genes had the largest variation, but the mutation of E gene has not been paid enough attention.
- keywords: acid; amino; analysis; avian; bayesian; birds; bronchitis; bronchitis virus; chickens; china; coronavirus; different; diversity; effective; entropy; evolutionary; field; figure; flocks; genes; genetic; genome; genotype; glycosylation; groups; guangxi; high; ibv; ibv isolates; ibvs; infectious; infectious bronchitis; isolates; ldt3; like; live; lsc/99i; lx4; major; minor; molecular; multiple; nanning; new; order; parent; past; phylogenetic; population; positive; predominant; pressure; previous; protein; recent; recombinants; recombination; reference; region; results; s1 gene; selection; sequences; sites; size; southern; southern china; strains; structural; study; subunit; supplementary; table; taiwan; time; type; type isolates; type strains; type vaccine; vaccine; variation; version; viral; virus; viruses; years
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- cord-318400-l9kwxsq7
- author: Chhabra, Rajesh
- title: Pathogenicity and tissue tropism of infectious bronchitis virus is associated with elevated apoptosis and innate immune responses
- date: 2016-01-15
- words: 5159
- flesch: 40
- summary: Early cellular and innate immune responses of virus infected cells in vitro could act as useful indicators for predicting the pathological outcome of viral infection in vivo. A variety of viruses have been shown to induce apoptosis in infected host cells (Clarke and Tyler, 2009; Shen and Shenk, 1995; Teodoro and Branton, 1997) .
- keywords: activity; apoptosis; apoptotic; assay; avian; bronchitis; cek; cek cells; cells; chicken; contrast; coronavirus; culture; disease; et al; expression; fig; gene; genotype; greater; host; hpi; ibv; ibv strains; ibvs; ifn; immune; induction; infected; infection; inflammatory; influenza; innate; levels; lgp2; like; m41; mda5; mock; moi; mrna; nephropathogenic; pathogenicity; pcr; po0.05; production; protein; regulation; renal; respiratory; response; rings; rna; significant; strains; study; time; tissue; tlr3; tocs; toll; total; tracheal; tropism; variant; viral; virus; viruses
- versions: original; plain text
- cord-319168-i23pqiwx
- author: Abro, Shahid Hussain
- title: Emergence of novel strains of avian infectious bronchitis virus in Sweden
- date: 2012-03-23
- words: 4935
- flesch: 52
- summary: Published IBV sequences of Massachusetts type, M41, H120, Baudette, Italy02, QX-like and 4/91 available in the GenBank were used to determine likely origin, genetic characteristics and molecular epidemiology of the Swedish isolates. Similar type of IBV isolates has been reported in Israel and Korea (Meir et al., 2004; Park et al., 2005) .
- keywords: acid; amino; analysis; available; avian; bronchitis; bronchitis virus; cases; cavanagh; china; complete; d388; data; deletions; different; disease; et al; events; gene; genotype; glycoprotein; group; ibv; infectious; infectious bronchitis; insertions; isolates; like; massachusetts; molecular; new; nucleotide; outbreaks; pcr; phylogenetic; positions; positive; poultry; pressure; recent; recombination; regions; related; selective; sequences; spike; spike gene; strains; strong; studies; study; subunit; sweden; swedish; swedish isolates; taiwan; vaccine; variants; virus
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- cord-319253-8bssrn9o
- author: OKINO, Cintia Hiromi
- title: Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I
- date: 2018-02-27
- words: 2507
- flesch: 29
- summary: A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus Rapid differentiation of avian infectious bronchitis virus isolates by sample to residual ratio quantitation using real-time reverse transcriptase-polymerase chain reaction Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Dengue typing assay based on real-time PCR using SYBR Green I LNA probe-based real-time RT-PCR for the detection of infectious bronchitis virus from the oviduct of unvaccinated and vaccinated laying hens The long view: 40 years of infectious bronchitis research Assessment of cross-immunity dm chickens to strains of avian infectious bronchitis virus using tracheal organ cultures Infectious bronchitis virus variants: a review of the history, current situation and control measures Increased level of protection of respiratory tract and kidney by combining different infectious bronchitis virus vaccines against challenge with nephropathogenic Brazilian genotype subcluster 4 strains Characterization of infectious bronchitis viruses isolated from outbreaks of disease in commercial flocks in Brazil SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses Nephritis associated with S1 variant brazilian isolate of infectious bronchitis virus and vaccine protection test in experimentally infected chickens A Real-Time Reverse-Transcription Polymerase Chain Reaction for Differentiation of Massachusetts Vaccine and Brazilian Field Genotypes of Avian Infectious Bronchitis Virus Vaccine efficacy against Ontario isolates of infectious bronchitis virus Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model The genotyping of infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain Review of infectious bronchitis virus around the world Serotype identification of avian infectious bronchitis virus by RT-PCR of the peplomer (S-1) gene Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus Development of a real-time TaqMan RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation Molecular epidemiology and evolution of avian infectious bronchitis virus Genetic grouping of avian infectious bronchitis virus isolated in Brazil, based on RT-PCR/RFLP analysis of the S1 gene Inflammatory and cell-mediated immune responses in the respiratory tract of chickens to infection with avian infectious bronchitis virus Detection of viral antigen following exposure of one-day-old chicken to the Holland-52 strain of IBV Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis Cluster analysis method for grouping means in the analysis of variance Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR I real-time PCR with nucleocapsid gene-specific primers Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of Plum pox virus strains C, EA, and W: effect of amplicon size, melt rate, and dye translocation Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan Massachusetts live vaccination protects against a novel infectious bronchitis virus S1 genotype DMV/5642/06 Antigenic and immunogenic characterization of infectious bronchitis virus strains isolated in China between ACKNOWLEDGMENTS.
- keywords: analysis; assay; avian; birds; bronchitis; detection; differentiation; disease; dpi; gene; genotype; green; h120; hvr; ibv; infected; infectious; isolates; isolation; m41; mass; melting; method; non; polymerase; qpcr; reaction; real; reverse; samples; strains; sybr; temperature; time; vaccine; virus
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- cord-321261-3lp54mmu
- author: Kuo, Shu-Ming
- title: Evolution of infectious bronchitis virus in Taiwan: Characterisation of RNA recombination in the nucleocapsid gene
- date: 2010-08-26
- words: 3888
- flesch: 48
- summary: Although previous studies of IBV S1 genes have argued that TW IBV is an independent branch of the US group (Huang et al., 2004) , the relationship between TW IBV and CH isolates remains unclear. TW IBV strains were isolated and propagated through the allantoic sac route by inoculating 9-11-day-old specific pathogens free (SPF) embryonated eggs from the Animal Health Research Institute, Council of Agriculture in Taiwan (Gelb and Jackwood, 1998) .
- keywords: algorithms; analysis; avian; bronchitis; cdna; china; coronavirus; data; detection; discordance; end; et al; evidence; evolution; evolutionary; fig; founder; gene; genome; high; huang; ibv; ibvs; incongruence; infectious; isolates; lai; length; n gene; new; phylogenetic; rdp; recombinant; recombination; region; results; rna; sars; sequences; similar; strains; study; taiwan; trees; tw ibv; tw2575/98; viral; virus; wang
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- cord-321545-3gzj09mr
- author: Pohuang, Tawatchai
- title: Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand
- date: 2009-09-07
- words: 2549
- flesch: 51
- summary: Group II consisted of eight isolates including THA30151, THA 70151, THA 80151, THA100151, THA110351, THA120351, THA130551, and THA140551, which had a close relationship with Chinese IBV isolates (strain A2, SH and QXIBV). Genotyping of IBV on the basis of the S1 gene sequence, particularly the HVR region of the S1 gene, is the most common way to classify IBV isolates.
- keywords: acid; allantoic; amino; amplification; analysis; bronchitis; chinese; database; field; genbank; gene; group; hvr; ibv; ibv isolates; ibvs; identities; infectious; isolates; molecular; new; nucleotide; pcr; primer; reaction; recent; region; s1 gene; sequence; serotypes; strains; thailand; typing; virus
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- cord-321602-88b2h06y
- author: Lv, Chenfei
- title: Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis
- date: 2020-08-19
- words: 4933
- flesch: 49
- summary: Construction scheme of a full-length cDNA clone of IBV H120 To construct the full-length cDNA of IBV H120 strain, total RNA was extracted from the allantoic fluid of SPF chicken embryonated eggs infected with H120 and transcribed to cDNA by reverse transcriptase using the Thermo Scientific RevertAid First-Strand cDNA Synthesis Kit (Thermo Fisher, USA). The procedure for the construction of full-length cDNA of IBV H120 has been shown in Fig. 1a .
- keywords: antibody; avian; bronchitis; cdna; cells; chickens; chimeric; clone; construction; control; coronavirus; day; dpi; eggs; et al; fig; fragments; gene; generation; genetic; genome; group; homologous; ibv; ibv h120; infected; infectious; length; method; old; pathogenicity; pbac; primers; protein; recombinant; recombination; rescue; results; reverse; rh120; rna; sc021202; scs1; sequence; spf; spike; strain; study; subcloning; system; table; vaccine; vector; virus; viruses
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- cord-322410-k23engcx
- author: Naguib, Mahmoud M.
- title: New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
- date: 2017-03-21
- words: 5022
- flesch: 43
- summary: Conventional RT-PCRs amplifying hypervariable regions (HVRs 1–2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples. No RT-PCR amplicates were obtained from two further samples that showed Cq values <32 in IBV RT-qPCR; failure to amplify IBV S1-specific RNA from these samples may be related to advanced RNA degradation.
- keywords: addition; aiv; amino; amplification; analysis; avian; bronchitis; characterization; chicken; clinical; conventional; copies; coronavirus; detection; different; disease; egypt; egyptian; entropy; et al; farms; fig; genbank; gene; genotypes; h5n1; h9n2; hvr; hvr3; hvrs; ibv; infectious; influenza; kit; molecular; naguib; ndv; newcastle; number; pathogens; pcrs; positive; poultry; presence; primers; protein; qpcr; reaction; real; regions; related; respiratory; rna; samples; sequences; serotypes; set; software; specific; specificity; spike; step; strains; study; table; time; vaccine; values; variant; viral; virus; viruses
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- cord-322516-wekvet6f
- author: Maceyka, Michael
- title: Ceramide Accumulation Uncovers a Cycling Pathway for the cis-Golgi Network Marker, Infectious Bronchitis Virus M Protein
- date: 1997-12-15
- words: 6196
- flesch: 48
- summary: We propose two models to explain the redistribution of IBV M protein induced by PDMP. Treatment of infected cells with lower concentrations of PDMP had no effect on IBV M localization and very little effect on SM synthesis (data not shown).
- keywords: accumulation; analogues; anterograde; antibodies; cells; ceramide; cgn; chase; cis; class; complex; control; cycle; cycloheximide; data; distribution; domains; effects; ergic-53; et al; experiments; fb1; fig; glucosylceramide; golgi; ibv; ibv m; immunofluorescence; indirect; infected; information; inhibitors; levels; lipids; localization; machamer; markers; membrane; min; pagano; pathway; pattern; pdmp; presence; pretreatment; protein; rate; retention; retrieval; rosenwald; secretory; sphingolipid; stack; staining; state; steady; synthesis; traffic; transmembrane; transport; virus; vsv
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- cord-323568-s0wmll4q
- author: Shang, Jian
- title: Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins
- date: 2018-04-23
- words: 7645
- flesch: 57
- summary: Specifically, because of the cross-subunit quaternary packing of trimeric IBV S1, HR1 and FP of IBV S2 are structurally restrained by two S1-CTDs from the other subunits and SD1 from another subunit, respectively (S5B Fig). Curiously, coronavirus S1 from different genera take two types of quaternary packing modes (Fig 5A-5D )
- keywords: area; binding; ceiling; cells; core; core structure; coronavirus; coronavirus s1; coronavirus spike; cov; cross; cryo; ctd; ctds; data; different; different genera; domain; ectodomain; electron; entry; evolutionary; features; fig; functional; fusion; genera; genus; host; hr1; hr2; human; ibv; ibv s1; ibv spike; immune; intra; loops; membrane; model; ntds; packing; pdb; post; pre; protein; putative; quaternary; rbms; receptor; recombinant; region; residues; sandwich; sars; sd1; sheet; site; spectrum; spike; state; structure; studies; study; subunit; sugar; surface; tag; terminal; topology; trimeric; viral
- versions: original; plain text
- cord-324324-8ybfiz8f
- author: Decaro, Nicola
- title: Novel human coronavirus (SARS-CoV-2): A lesson from animal coronaviruses
- date: 2020-04-14
- words: 14950
- flesch: 34
- summary: After 2002-2003 SARS epidemic, the renovated interest in HCoVs allowed the discovery of two additional viruses, the alphacoronavirus HCoV-NL63 and the betacoronavirus HCoV-HKU1, derived from bats and rodents, respectively (Tao et al., 2017) . NL63 (Tao et al., 2017) .
- keywords: ace2; acid; acute; adaptation; additional; alphacoronavirus; amino; analysis; ancestor; animals; antigenic; arabia; associated; avian; bats; bcov; betacoronavirus; binding; biological; birds; bovine; bronchitis; camels; canine; carnivores; cats; cause; cavanagh; ccov; cell; characterization; china; chinese; civets; clinical; common; corman; corman et; coronavirus; cov; cov-2; covs; cross; decades; decaro; decaro et; detected; detection; diarrhea; different; discovery; disease; divergent; diversity; dogs; domestic; dpp4; dromedary; east; embecovirus; emergence; enteric; epidemic; et al; european; event; evidence; evolution; evolutionary; fcov; feline; ferret; fip; gammacoronavirus; gastroenteritis; gene; genetic; genome; genomic; genus; group; hcov; hcov-229e; hedgehogs; high; hku4; horses; host; human; human coronavirus; ibv; identification; identity; important; infectious; isolation; italy; known; like; likely; lorusso; lorusso et; mammals; market; mechanisms; mers; mhv; mice; middle; mild; molecular; mouse; murine; natural; new; nl63; novel; novel coronavirus; nucleotide; oc43; order; origin; outbreak; pandemic; pathogenic; pedv; peritonitis; pigs; pneumonia; porcine; positive; prevalence; properties; protein; province; range; rats; rcov; recent; receptor; recombinant; recombination; related; reservoir; respiratory; respiratory coronavirus; respiratory disease; respiratory syndrome; responsible; review; rhinolophus; rna; rodents; role; rousettus; ruminants; sars; sars coronavirus; saudi; sequence; severe; species; spike; spp; strains; studies; study; subgenus; surveillance; susceptible; swine; syndrome; syndrome coronavirus; table; tcov; tgev; time; tissue; tract; transmissible; transmission; tropism; turkey; type; usa; veterinary; viral; viruses; wild; wildlife; woo; woo et; years; zoonotic
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- cord-326319-3538jmqd
- author: Yuan, Yuan
- title: Protection against Virulent Infectious Bronchitis Virus Challenge Conferred by a Recombinant Baculovirus Co-Expressing S1 and N Proteins
- date: 2018-06-27
- words: 7541
- flesch: 48
- summary: The expression of recombinant proteins S1, N and S1-N in Sf9 cells were detected using indirect immunofluorescence assay (IFA) and Western blot. The expression of recombinant proteins S1, N and S1-N in Sf9 cells were detected using indirect immunofluorescence assay (IFA) and Western blot.
- keywords: antibody; avian; bacmids rhbm; baculovirus; birds; bronchitis; cd4; cd8; cells; cellular; challenge; chickens; china; control; difference; dpb; dpc; dpv; dual; expression; figure; gene; group; h120; higher; ibv; immune; inactivated; infected; infectious; insect; isolation; levels; lymphocytes; n group; n protein; negative; pcr; percentages; pfast; post; primers; protection; proteins; rate; recombinant; responses; results; rhbm; s1 group; sf9; significant; specific; strain; study; subunit; transposon; vaccinated; vaccination; vaccines; vaccines rhbm; vector; virus; yl5
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- cord-328046-5us4se5o
- author: Xu, H. Y.
- title: Further Identification and Characterization of Novel Intermediate and Mature Cleavage Products Released from the ORF 1b Region of the Avian Coronavirus Infectious Bronchitis Virus 1a/1b Polyprotein
- date: 2001-09-30
- words: 5783
- flesch: 42
- summary: Colocalization of viral proteins with the organelle markers is represented by the yellow region within each cell in the merged images (C, F, I, L, and O). Replication and morphogenesis of avian coronavirus in Vero cells and their inhibition by monensin Impaired integrinmediated adhesion and signaling in fibroblasts expressing a dominant-negative mutant PTP1B Subcellular distribution of normal and mutant vitamin D receptors in living cells Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus Comparison of the replication of positive-stranded RNA viruses of plants and animals The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis Molecular chaperones Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase Protein folding in the cell Identification of an ATPase activity associated with a 71-kilodalton polypeptide encoded in gene 1 of the human coronavirus 229E Identification and subcellular localization of a 41 kDa, polyprotein 1ab processing product in human coronavirus 229E-infected cells Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Characterization of the two overlapping papain-like proteinase domains encoded in gene 1 of the coronavirus infectious bronchitis virus and determination of the C-terminal cleavage site of an 87 kDa protein Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by ORF 1a of the coronavirus avian infectious bronchitis virus and characterization of the cleavage products The missing link in coronavirus assembly: Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products Identification, expression, and processing of an 87-kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: Identification of a 10 kDa polypeptide and determination of its cleavage sites Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products Identification of a 24 kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage site Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a The human coronavirus 229E superfamily 1 helicase has RNA and DNA duplexunwinding activities with 5Ј-3Ј polarity Biochemical characterization of the equine arteritis virus helicase suggests a close functional relationship between arterivirus and coronavirus helicases Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells Mouse hepatitis virus replicase proteins associate with two distinct populations of intracellular membranes Characterization of endoplasmic reticulum by colocalization of B-p and dicarbocyanine dyes Localization of mouse hepatitis virus nonstructural proteins and RNA synthesis indicates a role for late endosomes in viral replication Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis Processing of the human coronavirus
- keywords: antisera; apparent; bronchitis; cells; cite; cleavage; cleavage products; coronavirus; course; detection; diffuse; distribution; et al; expression; fig; helicase; ibv; identification; immunoprecipitation; infectious; intermediate; kda; kda protein; lanes; like; liu; lysates; mature; molecular; nucleotides; p3c; pattern; plasmid; polyprotein; postinfection; processing; products; protein; proteinase; region; replication; rna; sites; stable; terminal; time; vero; viral; virus
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- cord-329245-6tj2k1yn
- author: Corse, Emily
- title: The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction
- date: 2003-07-20
- words: 7418
- flesch: 48
- summary: This would account for the small amount of IBV E protein relative to IBV M protein that is found in VLPs. We conclude that the cytoplasmic tail of IBV E protein is sufficient for interaction with M protein.
- keywords: amino; anti; antibodies; antibody; assembly; budding; cells; complex; coronavirus; corse; crosslinking; cytoplasmic; deletion; domain; dsp; eg3; envelope; fig; formation; golgi; ibv; ibv e; ibv m; immunoprecipitates; important; infected; interaction; lanes; m proteins; m2,3; machamer; mct; mhv; mutants; ost7; particles; pbs; presence; protein; rabbit; rat; region; results; small; supernatant; tail; transmembrane; type; vero; virus; viruses; vlp; vlps; vsv; wild
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- cord-329429-ur8g68vp
- author: Miłek, Justyna
- title: Coronaviruses in Avian Species – Review with Focus on Epidemiology and Diagnosis in Wild Birds
- date: 2018-12-10
- words: 3814
- flesch: 40
- summary: Emerg Infect Dis Emergence of a group 3 coronavirus through recombination Molecular identification and characterization of novel coronaviruses infecting graylag geese (Anser anser), feral pigeons (Columbia livia) and mallards (Anas platyrhynchos) Identification of avian coronavirus in wild aquatic birds of the Central and Eastern USA Surveillance of avian coronaviruses in wild bird populations of Korea Novel avian coronavirus and fulminating disease in guinea fowl Diverse gammacoronaviruses detected in wild birds from Madagascar First characterization of a Middle-East GI-23 lineage (Var2-like) of infectious bronchitis virus in Europe Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas) Retrospective testing and case series study of porcine delta coronavirus in US swine herds Identification of a novel coronavirus from a beluga whale by using a panviral microarray Broadly targeted multiprobe QPCR for detection of coronaviruses: coronavirus is common among mallard ducks (Anas platyrhynchos) Prevalence and phylogeny of coronaviruses in wild birds from the Bering Strait area (Beringia) Birds, migration and emerging zoonoses: West Nile Virus, Lyme disease, influenza A, and enteropathogens Reverse spillover of avian viral vaccine strains from domesticated poultry to wild birds Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay A Massachusetts prototype like coronavirus isolated from wild peafowls is pathogenic to chickens An avian coronavirus in quail withr respiratory and reproductive signs Gammacoronavirus and deltacoronavirus in quail Gamma and deltacoronaviruses in quail and pheasants from Northern Italy S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification A case for the ancient origin of coronaviruses The avian coronavirus spike protein Novel receptor specificity of avian gammacoronaviruses that cause enteritis Temporal dynamics, diversity, and interplay in three components of the virodiversity of a Mallard population: influenza A virus, avian paramyxovirus and avian coronavirus High prevalence and putative lineage maintenance of avian coronaviruses in Scandinavian waterfowl Urbanization and the dynamics of RNA viruses in Mallards (Anas platyrhynchos) Coronavirus diversity, phylogeny and interspecies jumping Comparative analysis of complete genome sequences of three avian coronaviruses reveals a novel group 3c coronavirus Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia Comparative analysis of complete genome sequences of three avian coronaviruses reveals a novel group 3c coronavirus Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus Genomic analysis and surveillance of the coronavirus dominant in ducks in China Moreover, CoVs other than IBVs, are identified in wild birds, which suggests that wild birds play a key role in the epidemiology of other gammaCoVs and deltaCoVs.
- keywords: analysis; avian; binding; birds; bronchitis; cell; chickens; complete; coronaviruses; covs; deltacoronavirus; deltacovs; detection; different; disease; diversity; domestic; ducks; emergence; gamma; gammacoronavirus; gammacovs; gene; genome; genomic; genus; high; host; humans; ibv; ibvs; identification; infectious; large; like; molecular; monitoring; new; novel; poultry; presence; proteins; receptor; recombination; regions; replicase; respiratory; rna; role; sars; sequence; similar; species; specificity; strains; studies; taxonomic; tcov; viral; wild; wild birds
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- cord-329468-vjsurl60
- author: Okino, Cintia Hiromi
- title: Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
- date: 2017-02-15
- words: 5854
- flesch: 41
- summary: In contrast, in the current study an unexpected suppressive effect in the TLR7 gene expression was observed only during the early phase of IBV B isolate infection (1dpi), as the transcripts of this gene were found 5 times downregulated. Prevention of IBV infection is currently achieved through vaccination, especially by attenuated viral vaccines, suggesting that local mucosal immunity is essential for induction of effective protection against disease [7, 8, 9] .
- keywords: 1dpi; 5dpi; activation; adaptive; avian; b isolate; birds; bronchitis; cd3; cells; challenged; changes; chickens; cmi; degeneration; differences; different; disease; early; epithelial; expression; fig; genes; genome; granzyme; group; higher; homolog; host; ibv; ibv b; ifnα; ifnβ; ifnγ; immune; immune responses; infected; infection; inflammatory; innate; inos; isolate; kidney; lesions; levels; load; microscopic; mrna; pathogenesis; pathological; positive; post; presence; renal; replication; respiratory; responses; samples; scores; significant; strain; study; table; tissue; tlr3; tlr7; tracheal; tracheal samples; transcripts; upregulated; viral; virus
- versions: original; plain text
- cord-329497-3jow4xbn
- author: Promkuntod, Naruepol
- title: Dynamics of avian coronavirus circulation in commercial and non-commercial birds in Asia – a review
- date: 2015-12-28
- words: 8021
- flesch: 45
- summary: Germany: Rauischholzhausen Isolation and characterization of a coronavirus from pigeons with pancreatitis Outbreaks of infectious bronchitis in Korea Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection Molecular survey of avian respiratory pathogens in commercial broiler chicken flocks with respiratory diseases in Jordan Molecular subtype of infectious bronchitis virus in broiler flocks in Jordan A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran Complete nucleotide sequences of S1 and N genes of infectious bronchitis virus isolated in Japan and Taiwan Isolation of 4/91 type of infectious bronchitis virus as a new variant in Japan and efficacy of vaccination against 4/91 type field isolate Genetic analysis of the S1 gene of 4/91 type infectious bronchitis virus isolated in Japan /B type, the predominant circulating type of avian infectious bronchitis viruses 1999À2004 in Iran: a retrospective study Associations between attributes of live poultry trade and HPAI H5N1 outbreaks: a descriptive and network analysis study in northern Vietnam Sequencing, phylogenetic analysis, and potential recombination events of infectious bronchitis viruses isolated in Korea Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India A Massachusetts prototype like coronavirus isolated from wild peafowls is pathogenic to chickens Epidemiology and control of avian influenza H5N1 virus in Thailand ISRN Vet Sci Studies on avian viral infections in Iran Detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in Pakistan Circulation of QX-like infectious bronchitis virus in the Middle East Changes of several acute phase factors in broiler chickens in response to infectious bronchitis virus infection Emerging viral diseases of livestock in the developing world Emergence of a nephropathogenic avian infectious bronchitis virus with a novel genotype in India Evidence of circulation of a Chinese strain of infectious bronchitis virus (QXIBV) in Italy Different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in China Molecular epizootiology of avian infectious bronchitis in Russia Pathogenesis and tissue distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/B serotype) in experimentally infected broiler chickens Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses Coronaviruses in poultry and other birds Coronavirus avian infectious bronchitis virus Infectious bronchitis Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan Identification of intertypic recombinant infectious bronchitis viruses from slaughtered chickens Studies on the attenuated of infectious bronchitis virus Village chicken production systems in Thailand Avian coronavirus in wild aquatic birds A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B) Infectious bronchitis virus variants: a review of the history, current situation and control measures Progression of lesions in the respiratory tract of broilers after single infection with Escherichia coli compared to superinfection with E. coli after infection with infectious bronchitis virus Virulent avian infectious bronchitis virus, People's Republic of China S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel Infectious bronchitis virus serotypes in poultry flocks in Jordan A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China Complete genome sequence of an infectious bronchitis virus chimera between cocirculating heterotypic strains S1 and N gene analysis of avian infectious bronchitis viruses in Taiwan Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains Sequence changes of infectious bronchitis virus isolates in the 3 0 7.3 kb of the genome after attenuating passage in embryonated eggs Studies on broiler's IBV and IBV-like from guinea fowl Review of infectious bronchitis virus around the world Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during Identification of recent infectious bronchitis virus isolates that are serologically different from current vaccine strains Genetic diversity of avian infectious bronchitis virus isolates in Korea between S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea Sequence analysis of nephropathogenic infectious bronchitis virus strains of the Massachusetts genotype in Beijing Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in south China during Continuous evolution of avian infectious bronchitis virus resulting in different variants co-circulating in southern China Serotype and genotype diversity of infectious bronchitis viruses isolated during An emerging recombinant cluster of nephropathogenic strains of avian infectious bronchitis virus in Korea Infectious bronchitis virus:
- keywords: acovs; addition; analysis; asia; avian; avian infectious; backyard; birds; broiler; bronchitis; bronchitis virus; chickens; china; chinese; circulation; commercial; common; control; coronavirus; countries; different; disease; diversity; emergence; et al; example; factors; farming; farms; field; flocks; form; gene; genetic; genotype; group; h120; h5n1; ibv; ibv strains; ibvs; important; infectious; infectious bronchitis; influenza; isolated; isolation; japan; kidney; korea; later; like; liu; live; local; mass; molecular; nephropathogenic; new; non; novel; outbreaks; phylogenetic; poultry; predominant; present; prevalent; production; protein; recent; recombination; respiratory; review; sequence; severe; signs; species; spread; strains; taiwan; taiwanese; thailand; trading; transmission; type; vaccinated; vaccination; vaccines; variants; virus; viruses
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- cord-330057-3vucm0s1
- author: Franzo, Giovanni
- title: Phylodynamic analysis and evaluation of the balance between anthropic and environmental factors affecting IBV spreading among Italian poultry farms
- date: 2020-04-29
- words: 5537
- flesch: 32
- summary: The inclusion of a third deme in the model revealed the likely role of other poultry companies and rural farms (particularly concentrated in Northern Italy) as sources of strain introduction into one of the major poultry companies, whose farms are mainly located in the high densely populated poultry area of Northern Italy. The strains from different poultry companies formed two independent clusters, which suggests the effectiveness of independent production flow/chain in protecting farms from exogenous introductions.
- keywords: 95hpd; analysis; approach; area; available; avian; bronchitis; circulation; coalescent; collected; companies; company; company b; considered; continuous; control; dataset; deme; density; different; dispersal; distribution; effective; environmental; epidemiology; estimated; factors; farms; field; fig; genotype; ghost; high; history; ibv; increase; infectious; influenza; information; integrated; introduction; italian; italy; management; measures; migration; model; molecular; northern; parameters; period; phylogenetic; populated; population; posterior; poultry; poultry companies; presence; present; region; relevant; results; risk; role; samples; sampling; sector; sequences; size; spatial; spreading; strains; structured; study; time; transmission; tree; vaccination; velocity; viral; virus
- versions: original; plain text
- cord-330260-xuw31zfn
- author: Chen, Hui-Wen
- title: Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan
- date: 2009-01-20
- words: 3715
- flesch: 49
- summary: In this study, the 3 7.3 kb genomes from Taiwan IBV strains were investigated to elucidate the genetic diversity of viruses. Evidence of circulation of a Chinese strain of infectious bronchitis virus (QXIBV) in Italy Molecular epizootiology of avian infectious bronchitis in Russia Phylogenetic analysis of partial S1 and N gene sequences of infectious bronchitis virus isolates from Italy revealed genetic diversity and recombination Genes 3 and 5 of infectious bronchitis virus are accessory protein genes Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes Infectious bronchitis virus S2 gene sequence variability may affect S1 subunit specific antibody binding Co-circulation of four types of infectious bronchitis virus (793/B, 624/I, B1648 and Massachusetts)
- keywords: 3374/05; analyses; avian; bronchitis; cavanagh; china; coronavirus; crossover; et al; events; field; gene; genetic; genome; glycoprotein; group; h120; ibv; ibvs; identity; infectious; infectious bronchitis; isolates; ldl/97i; liu; local; molecular; nucleotide; parental; phylogenetic; poultry; protein; putative; recombinants; recombination; region; rna; sequence; spike; strains; structural; study; taiwan; vaccine; variants; viral; virus; viruses; wang
- versions: original; plain text
- cord-331020-lyxje82u
- author: M. Najimudeen, Shahnas
- title: Infectious Bronchitis Coronavirus Infection in Chickens: Multiple System Disease with Immune Suppression
- date: 2020-09-24
- words: 6968
- flesch: 31
- summary: Viruses Antigenic and Pathogenic Characteristics of QX-Type Avian Infectious Bronchitis Virus Strains Isolated in Southwestern China Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain Heparan Sulfate Is a Selective Attachment Factor for the Avian Coronavirus Infectious Bronchitis Virus Beaudette Sialic acid is a receptor determinant for infection of cells by avian Infectious bronchitis virus Binding of avian coronavirus spike proteins to host factors reflects virus tropism and pathogenicity Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding The pathogenesis of nephritis in chickens induced by infectious bronchitis virus Pathogenesis of Oviduct Lesions in Immature Chickens Following Exposure to Infectious Bronchitis Virus at One Day Old A 'new' strain of infectious bronchitis virus infecting domestic fowl in Great Britain Immunopathogenesis of infection in SPF chicks and commercial broiler chickens of a variant infectious bronchitis virus of economic importance Infectious bronchitis Nephropathogenicity of infectious bronchitis virus The etiology of uraemia of chickens Infectious avian nephrosis (uraemia) in Australia Isolation of avian infectious bronchitis virus from experimentally infected chickens In vitro characterization and pathogenesis of Egypt/Beni-Suef/01; a novel genotype of infectious bronchitis virus Establishment of Persistent Avian Infectious Bronchitis Virus Infection in Antibody-Free and Antibody-Positive Chickens Avian infectious bronchitis: Viral persistence in the harderian gland and histological changes after eyedrop vaccination Pathogenicity of a TW-Like Strain of Infectious Bronchitis Virus and Evaluation of the Protection Induced against It by a QX-Like Strain Comparison of the susceptibility to avian infectious bronchitis virus infection of two inbred lines of white leghorn chickens Electron microscope observations on the entry of avian infectious bronchitis virus into susceptible cells Histopathology and immunohistochemistry of renal lesions due to infectious bronchitis virus in chicks Detection of viral antigen following exposure of one-day-old chickens to the Holland 52 strain of infectious bronchitis virus Isolation of 4/91 Type of Infectious Bronchitis Virus as a New Variant in Japan and Efficacy of Vaccination Against 4/91 Type Field Isolate Productive replication of nephropathogenic infectious bronchitis virus in peripheral blood monocytic cells, a strategy for viral dissemination and kidney infection in chickens Tissue Tropism of Three Cloacal Isolates and Massachusetts Strain of Infectious Bronchitis Virus Pathogenesis and tissue distribution of avian infectious bronchitis virus isolate IRFIBV32 Experiences of vaccination against avian infectious bronchitis coronavirus The Effect of Infectious Bronchitis Virus on Chickens Infected with Mycoplasma gallisepticum Synergism between avian infectious bronchitis virus and Haemophilus gallinarum Effect of in ovo bursectomy on the course of an infectious bronchitis virus infection in line C White Leghorn chickens Cytotoxic T cells are critical in the control of infectious bronchitis virus in poultry Specific antibody secreting cells from chickens can be detected by three days and memory B cells by three weeks post-infection with the avian respiratory coronavirus Memory T cells protect chicks from acute infectious bronchitis virus infection Infectious Bronchitis Virus Immune Responses in the Harderian Gland upon Initial Vaccination Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain Disease of Poultry Infectious bronchitis Maternal antibody to infectious bronchitis virus: Its role in protection against infection and development of active immunity to vaccine Infectious bronchitis in laying hens: Interference with response to emulsion vaccine by attenuated live vaccine Studies with a bivalent infectious bronchitis killed virus vaccine Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: A review Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain Safety and efficacy of an infectious bronchitis virus used for chicken embryo vaccination Infectious bronchitis Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene Genetic Analysis of the S1 Gene of 4/91 Type Infectious Bronchitis Virus Isolated in Japan Transmissibility of Infectious Bronchitis Virus H120 Vaccine Strain among Broilers under Experimental Conditions Molecular epizootiology of infectious bronchitis virus in Sweden indicating the involvement of a vaccine strain Ion channel activity of influenza A virus M2 protein: Characterization of the amantadine block Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures Different evolutionary trajectories of vaccine-controlled and non-controlled avian infectious bronchitis viruses in commercial poultry Avian coronavirus infectious bronchitis attenuated live vaccines undergo selection of subpopulations and mutations following vaccination Hatchery Vaccination Against Poultry Viral Diseases: Potential Mechanisms and Limitations Genotyping of infectious bronchitis viruses identified in Canada between Vaccine efficacy against Ontario isolates of infectious bronchitis virus Using phylogenetic analysis to examine the changing strains of infectious bronchitis virus infections in Ontario over time Vaccination against infectious bronchitis virus: A continuous challenge Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge Etiology and immunology of infectious bronchitis virus Comparison of the pathogenicity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license The authors declare no conflict of interest.
- keywords: adaptive; addition; antibody; attenuated; available; avian; avian infectious; b1648; blood; bronchitis; bronchitis virus; capable; cause; cells; certain; changes; chickens; chicks; clinical; complement; conn; control; coronavirus; day; dependent; development; different; disease; dissemination; dpi; early; egg; epithelium; evidence; example; expression; functions; host; ibv; ibv infection; ibv strains; ifns; immune; inactivated; infected; infectious; infectious bronchitis; innate; kidney; layer; lead; lesions; like; live; low; lysis; macrophages; manifestations; mass; mechanisms; molecular; monocytes; nephropathogenic; organs; oviduct; pathogenesis; pathogenicity; pathological; persistence; poultry; production; protein; receptors; replication; reproductive; respiratory; response; serotypes; severe; strains; strategies; studies; system; tissues; tract; tropism; type; vaccination; vaccines; viral; virus; viruses
- versions: original; plain text
- cord-331740-yjt3q9ph
- author: Jones, R. M.
- title: Development and Validation of RT‐PCR Tests for the Detection and S1 Genotyping of Infectious Bronchitis Virus and Other Closely Related Gammacoronaviruses Within Clinical Samples
- date: 2011-04-07
- words: 5873
- flesch: 41
- summary: Transport and initial processing of field samples Tissue samples (kidney, brain, liver, oviduct, bursa and respiratory and intestinal tracts) were collected from a variety of bird species (chicken, turkey, pheasant and pigeon) at post-mortem from cases with respiratory disease or suspected coronavirus infection based on clinical observations. The IBV S1 sequence files were taken from the public access Genbank database and included representatives of the major groups of IBV strains that are currently circulating in Europe alongside other IBV strains (see Fig. 1 ).
- keywords: acid; amplicons; amplification; analysis; assay; avian; birds; bronchitis; bronchitis virus; cavanagh; chickens; coronavirus; data; detection; diagnostic; different; disease; eggs; et al; field; field samples; following; gammacoronaviruses; gene; genotyping; ibv; ibv real; ibv strains; identification; infectious; infectious bronchitis; isolation; laboratory; method; molecular; nucleic; panel; pcr; positive; present; primer; probe; reaction; real; region; results; rev; reverse; rna; samples; sequence; sequencing; serotypes; step; strains; suitable; swabs; taqman; template; test; testing; time; time rt; turkey; use; vaccine; viral; virus; virus isolation
- versions: original; plain text
- cord-334090-66d8c75g
- author: Seger, Waleed
- title: Genotyping of infectious bronchitis viruses from broiler farms in Iraq during 2014-2015
- date: 2016-02-18
- words: 3624
- flesch: 49
- summary: Molecular detection of infectious bronchitis virus and it is relation with avian influenza virus (H9) and Mycoplasma gallisepticum from different geographical regions in Iraq Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry Circulation of QX-like infectious bronchitis virus in the Middle East Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus Detection of the Chinese genotype of infectious bronchitis virus (QX-type) in Iran Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Coronaviruses in poultry and other birds Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs Factors influencing the outcome of infectious bronchitis vaccination and challenge experiments Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during Genotypes of infectious bronchitis viruses circulating in the Middle East between Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: a review Phylogenetic study of Iranian infectious bronchitis virus isolates during 2010-2011 using glycoprotein S1 gene Review of infectious bronchitis virus around the world Efficacy of live infectious bronchitis vaccines against a novel European genotype, Italy 02 Development and validation of RT-PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gammacoronaviruses within clinical samples Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey Evolutionary and bioinformatics analysis of the spike glycoprotein gene of H120 vaccine strain protectotype of infectious bronchitis virus from India Fenner's veterinary virology Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Infectious Bronchitis Virus: a major cause of respiratory disease outbreaks in chickens in Ghana Identification of a novel nephropathogenic infectious bronchitis virus in Israel Complete genome sequences of two Chinese virulent avian coronavirus infectious bronchitis virus variants Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand Investigation and molecular characterization of avian infectious bronchitis virus in suspected broiler farms in Slemani Governorate. key: cord-334090-66d8c75g authors: Seger, Waleed; GhalyanchiLangeroudi, Arash; Karimi, Vahid; Madadgar, Omid; Marandi, Mehdi Vasfi; Hashemzadeh, Masoud title: Genotyping of infectious bronchitis viruses from broiler farms in Iraq during 2014-2015 date: 2016-02-18 journal: Arch Virol DOI: 10.1007/s00705-016-2790-2 sha: doc_id: 334090 cord_uid: 66d8c75g Infectious bronchitis virus (IBV) is one of the most critical pathogens in the poultry industry, causing serious economic losses in all countries including Iraq.
- keywords: analysis; avian; broiler; bronchitis; chickens; china; countries; detection; different; dy12; east; farms; field; flocks; gene; genotype; genotyping; governorate; group; ibv; infectious; iran; iraq; is/1494; isolates; like; middle; min; molecular; pcr; phylogenetic; positive; poultry; rate; respiratory; samples; sequences; sequencing; sinaclon; spread; strains; study; time; variant; virus; viruses
- versions: original; plain text
- cord-335310-61wibso4
- author: Chen, Hui-Wen
- title: Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection
- date: 2016-08-15
- words: 5491
- flesch: 35
- summary: The result demonstrates the feasibility of studying the evolution of nanoparticle protein corona formation using nanoparticle tracking analysis, which reveals changes in light scattering and size simultaneously. structural characterization of the spike protein From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis A truncated receptorbinding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody A simple method of estimating fifty per cent endpoints A Laboratory Manual for the Isolation and Identification of Avian Pathogens Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan Quantitative profiling of the protein coronas that form around nanoparticles Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))
- keywords: 2575/98; analysis; antigen; aunps; avian; biological; bronchitis; buffer; cells; cellular; challenge; chen; chicken; comparison; corona; coronavirus; day; delivery; development; different; disease; display; features; fig; formation; formulations; free; gold; group; high; humoral; ibv; iga; igg; immune; immunity; improved; increased; incubation; infectious; interactions; invitrogen; kidneys; light; like; lymph; lymphatic; manufacturer; method; mice; min; model; nanoparticles; native; pbs; pcr; pdi; present; protection; protein; purified; recombinant; responses; room; samples; size; solution; specific; spike; staining; study; surface; svlps; synthetic; taiwan; temperature; tracking; vaccination; vaccine; viral; virions; virus; washes; wiv
- versions: original; plain text
- cord-337128-yyz7z0xj
- author: Abdel-Moneim, Ahmed S
- title: Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos
- date: 2009-02-05
- words: 3314
- flesch: 38
- summary: key: cord-337128-yyz7z0xj authors: Abdel-Moneim, Ahmed S; Zlotowski, Priscila; Veits, Jutta; Keil, Günther M; Teifke, Jens P title: Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos date: 2009-02-05 journal: Virol J DOI: 10.1186/1743-422x-6-15 sha: doc_id: 337128 cord_uid: yyz7z0xj BACKGROUND: Infectious bronchitis virus primarily induces a disease of the respiratory system, different IBV strains may show variable tissue tropisms and also affect the oviduct and the kidneys. Xth Wld Vet Poult Assoc Congress American Association of Avian Pathologists Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction-fragment-length polymorphism analysis Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene Detection of infectious bronchitis virus Genetic differences among chicken embryos in response to inoculation with an isolate of infectious bronchitis virus Use of allantoic cells for the detection of avian infectious bronchitis virus Comparative growth kinetic studies on avian infectious bronchitis virus in different systems Infectious bronchitis virus replication in the chicken embryo related cell line A monoclonal antibody-based immunoperoxidase procedure for rapid detection of infectious bronchitis virus in infected tissues Avian infectious bronchitis virus distribution in tissues of chicks The pathogenesis of nephritis in chicks induced by infectious bronchitis virus Tissue tropism of three cloacal isolates and Massachusetts strain of infectious bronchitis virus Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos Nephrosis in laying chickens caused by Massachusetts-type infectious bronchitis virus S1 gene sequence analysis of a nephro-pathogenic strain of avian infectious bronchitis virus in Egypt In vitro characterization and pathogenesis of Egypt/Beni-Suef/01; a novel genotype of infectious bronchitis virus Early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus Detection of in ovo-inoculated infectious bronchitis virus by immunohistochemistry and in situ hybridization with a riboprobe in epithelial cells of the lung and cloacal bursa Tissue distribution of avian infectious bronchitis virus following in ovo inoculation of chicken embryos examined by in situ hybridization with antisense digoxigenin-labeled universal riboprobe The control of avian infectious bronchitis/nephrosis in Australia Establishment of persistent avian infectious bronchitis virus infection in antibodyfree and antibody-positive chickens Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and nonvaccinated flocks in China The quail and chicken intestine have sialyl-galactose sugar chains responsible for the binding of influenza A viruses to human type receptors Properties and developmental regulation of polysialyltransferase activity in the chicken embryo brain Developmental regulation of polysialic acid synthesis in mouse directed by two polysialyltransferases, PST and STX Cell culture methods Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons We thank Katrin Giesow and Gabriele Czerwinski for excellent technical assistance.
- keywords: acid; allantoic; antigen; avian; bronchitis; cam; cek; cells; chicken; days; detection; different; distribution; ece; eggs; embryonic; embryos; epithelium; figure; fluid; ibv; ihc; immunohistochemistry; infected; infectious; inoculated; inoculation; isolated; isolation; kidney; lung; m41; neurons; positive; present; proventriculus; respiratory; serum; sialic; spleen; strain; study; system; tissues; titers; trachea; tract; tropism; virus
- versions: original; plain text
- cord-337441-c5bxthwn
- author: You, Jae-Hwan
- title: Three-Dimensional Reconstruction of the Nucleolus Using Meta-Confocal Microscopy in Cells Expressing the Coronavirus Nucleoprotein
- date: 2006
- words: 1760
- flesch: 42
- summary: For example, compare the distribution of IBV N protein to the PI stained nucleolus in optical section 2.64 µm (Fig. 2, arrows) , N protein would appear to occupy less nucleolar volume. META-confocal analysis and three-dimensional reconstructions of cells expressing IBV N protein revealed that N protein does not localize throughout the nucleolus and may be confined to the DFC.
- keywords: cells; confocal; coronavirus; cov; cytoplasm; data; ecfp; egfp; emission; fig; gene; ibv; laser; line; live; localization; meta; microscopy; nuclear; nucleolar; nucleolus; nucleus; protein; sars; structure; viral
- versions: original; plain text
- cord-338307-vfutmwxq
- author: Sturman, Lawrence S.
- title: The Molecular Biology of Coronaviruses
- date: 1983-12-31
- words: 21989
- flesch: 46
- summary: Although in moderate infection of MHV-A59 in 17 C1 1 cells, little or no 90K E2 was detected at 8-10 hours after infection (Holmes et al., 1981b; Fig. 16) , in Sac ( -) cells infected with MHV-JHM or -A59, a substantial amount of 90K E2 was detected after 60-to 90-minute chase (Siddell et al., 1981c; Rottier et al., 1981b) . The same conclusion was reached by others (Holmes et al., 1981a,b; Siddell et al., 1981c; Rottier et al., 1981b) based on the resistance of the glycosylation of E l to tunicamycin, an inhibitor specific for N-glycosylation.
- keywords: 1981b; a59; acid; activity; additional; analysis; antibody; antigenic; apparatus; associated; avian; bang; bcv; binding; biological; biology; bovine; bronchitis; bronchitis virus; budding; cells; characterization; characterized; cleavage; comparison; composition; conditions; coronavirus; cross; culture; cycle; data; dependent; diameter; differences; different; disease; disulfide; e l; early; effects; electron; end; endoplasmic; enteric; envelope; et al; evidence; features; fig; form; formation; fucose; functions; fusion; garwes; genetic; genome; glucosamine; glycoprotein; glycosylated; glycosylation; golgi; growth; hcv; hcv-229e; helical; hepatitis; hepatitis virus; hev; high; holmes; holmes et; host; hours; human; ibv; important; inclusions; infected; infected cells; infection; infectivity; influenza; inhibited; interaction; intracellular; investigators; isolated; isolation; jhm; kennedy; klenk; lai; lai et; large; leibowitz; like; line; macnaughton; macrophages; manaker; membrane; mhv; mice; microscopic; model; molecular; monospecific; morphology; mouse; mouse hepatitis; mrnas; murine; mutants; negative; new; niemann; nucleocapsid; oc43; oligonucleotide; particles; peplomers; permission; plaque; plasma; polymerase; polypeptides; porcine; presence; present; product; properties; proteins; proteolytic; purified; related; relationships; release; replication; residues; resistant; reticulum; rna; role; rottier et; schmidt; sds; section; sensitive; sequence; siddell et; similar; single; size; small; species; specific; stern; stohlman; strain; structural; studies; study; sturman; subgenomic; sulfate; surface; susceptibility; susceptible; synthesis; temperature; tgev; time; tissue; transcription; transformed; translation; transport; treatment; trypsin; tunicamycin; type; tyrrell; uitro; unpublished; vesicles; viral; virions; virus; viruses; wege et
- versions: original; plain text
- cord-339235-8xslz4bs
- author: Boroomand, Zahra
- title: Molecular detection and phylogenetic properties of isolated infectious bronchitis viruses from broilers in Ahvaz, southwest Iran, based on partial sequences of spike gene
- date: 2018-09-15
- words: 1979
- flesch: 47
- summary: Genotyping of Avian infectious bronchitis viruses in Iran (2015-2017) reveals domination of IS-1494 like virus Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran The pathogenesis of a new variant genotype and QX-like infectious bronchitis virus isolated from chickens in Thailand Circulation of QXlike infectious bronchitis virus in the Middle East Genotyping of infectious bronchitis viruses from broiler farms in Iraq during Detection of the Chinese genotype of infectious bronchitis virus (QX-type) in Iran Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East One of the major concerns regarding to IB is the emergence of new types of infectious bronchitis viruses (IBVs).
- keywords: ahvaz; allantoic; avian; broiler; bronchitis; chickens; different; flocks; gene; genotype; ibv; ibvs; infectious; iran; isolates; like; molecular; new; pcr; phylogenetic; positive; present; respiratory; sequences; serotypes; strains; study; vaccination; virus; viruses
- versions: original; plain text
- cord-339259-4oi7slk9
- author: Naguib, Mahmoud M.
- title: Full genome sequence analysis of a newly emerged QX-like infectious bronchitis virus from Sudan reveals distinct spots of recombination
- date: 2016-10-26
- words: 4021
- flesch: 41
- summary: The Veterinary record Complete genomic sequence analysis of infectious bronchitis virus ark dpi strain and its evolution by recombination Isolation and characterization of infectious bronchitis virus strain 4/91 from commercial layer chickens in the sudan Serosurveillance study on avian infectious bronchitis virus in Sudan Mechanisms of coronavirus cell entry mediated by the viral spike protein Development and evaluation of a real-time taqman rt-pcr assay for the detection of infectious bronchitis virus from infected chickens Severe acute respiratory syndrome vaccine development: Experiences of vaccination against avian infectious bronchitis coronavirus Coronaviruses in poultry and other birds Coronavirus avian infectious bronchitis virus Infectious bronchitis virus: Avian pathology: journal of the W Avian infectious bronchitis virus Review of infectious bronchitis virus around the world A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza a virus Mafft multiple sequence alignment software version 7: improvements in performance and usability Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data Evolution of infectious bronchitis virus in Taiwan: characterisation of rna recombination in the nucleocapsid gene Recombination in large rna viruses A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China.
- keywords: accession; acids; africa; amino; analysis; ar251; avian; bronchitis; cavanagh; characterization; chickens; china; complete; coronavirus; data; detection; different; disease; et al; europe; fig; gene; genome; genotype; germany; h120; highest; hvr3; hvrs; ibv; identities; identity; infectious; isolate; like; new; nucleotide; orf; phylogenetic; poultry; protein; rdp4; recombination; regions; respiratory; rna; sequence; sequencing; serotype; software; specific; spike; strains; study; sudan; sudanese; vaccine; virus; viruses
- versions: original; plain text
- cord-341541-3l6tjf3t
- author: Hajijafari Anaraki, Mozafar
- title: Molecular characterization of infectious bronchitis virus based on RNA‐dependent RNA polymerase gene
- date: 2020-05-26
- words: 1276
- flesch: 43
- summary: Based on the RT-PCR detection and sequence analysis of IBV RdRp gene, an overall prevalence of field strains estimated as 44.2%. Phylogenetic analysis of RdRp gene sequences resulted in clustering the IBV strains related to each area.
- keywords: analysis; article; avian; bronchitis; characterization; copyright; detection; gene; ibv; infectious; main; molecular; pcr; phylogenetic; polymerase; rdrp; replicase; rights; rna; sequences; sequencing; strains; vaccine; variants; variations; virus
- versions: original; plain text
- cord-342176-tewfm8it
- author: Kjærup, Rikke M.
- title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations
- date: 2013-11-08
- words: 8402
- flesch: 53
- summary: Purified chicken MBL was bought from the Department of Cancer and Inflammation Research, University of Southern Denmark. The addition of MBL ligands to the IBV vaccine, especially FOS, enhanced the production of IBV-specific IgG antibody production in L10H chickens, but not L10L chickens after the second vaccination.
- keywords: absolute; adaptive; addition; animal; antibodies; antibody; attenuated; binding; blood; bronchitis; cat; cd4−cd8; cells; chickens; chitosan; concentrations; counts; current; deficiency; denmark; difference; dose; effect; et al; fig; flow; fos; group; higher; ibv; igg; ijk; immune; immunity; increase; infection; innate; juul; juulmadsen; l10h; l10l; l10l chickens; lectin; ligand; live; load; lower; madsen; mannan; mannose; mbl; mbl ligand; mice; min; number; pcr; production; prrs; purified; pv1; qrt; recognition; response; results; samples; second; serum; significant; specific; studies; study; sublines; titres; total; treatment; treatment group; vaccination; vaccine; viral; virus; water; week
- versions: original; plain text
- cord-342354-j10m2dfh
- author: Chen, Huijie
- title: Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells
- date: 2019-12-31
- words: 5879
- flesch: 40
- summary: A total of 3 different experimental designs: (1) pretreatment of cells prior to infection; (2) direct treatment of virus-infected cells; and (3) pre-treatment of virus prior to infection were used to analyze the antiviral activity of HY on CEK cells infection by IBV (De La Torre et al., 2011; Yin et al., 2011) . While, at the same drug concentration, the virus titer was the lowest when HY directly treated the infected virus cells, followed by the group of HY pre-treatment virus prior to infection, and followed by the group of HY pretreatment cells prior to infection.
- keywords: activity; agricultural; annexin; antiviral; apoptosis; apoptotic; assay; bax; bronchitis; caspase; cek; cek cells; cells; chicken; china; concentration; control; coronavirus; cultured; data; different; direct; dmso; effect; embryo; et al; experimental; expression; fasl; figure; fluorescence; gene; group; hypericin; hypericum; ibv; ibv infection; infected; infection; inhibition; inhibitory; jnk; kidney; levels; m199; m41; medium; mock; mrna; mrna expression; mtt; pcr; perforatum; production; protein; related; relative; results; ros; significant; solution; strain; stress; tcid50; titer; total; treatment; usa; veterinary; virus
- versions: original; plain text
- cord-343893-sophqqne
- author: Chu, Victor C
- title: Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus
- date: 2007-02-26
- words: 3976
- flesch: 49
- summary: Kidney cells were washed in 50 ml sterile phosphate buffer saline (PBS) twice via gentle stirring to remove red blood cells. Cell supernatant was mixed with 1 ml of FBS and centrifuged at 1,000 rpm for 2 min. Media were removed and kidney cells were resuspended in 25 ml of M20 media.
- keywords: acid; acute; aminopeptidase; ark_dpi; avian; bronchitis; ca99; canine; cells; chicken; conn46; coronavirus; covs; crfk; entry; expression; family; fapn; feline; field; fig; fipv; fipv-1146; functional; group; h52; host; human; ibv; ibv_ark99; ibv_mass41; infected; infection; influenza; iowa97; kidney; monoclonal; monolayers; non; potential; primary; prototype; receptor; respiratory; sars; sialic; species; strains; studies; study; syndrome; tgev; university; utilization; viral; virus; viruses
- versions: original; plain text
- cord-344297-qqohijqi
- author: Smith, Jacqueline
- title: The early immune response to infection of chickens with Infectious Bronchitis Virus (IBV) in susceptible and resistant birds
- date: 2015-10-09
- words: 5080
- flesch: 46
- summary: An apparently new respiratory disease in baby chicks Studies of infectious coryza of chickens with special reference to its etiology Cultivation of the virus of infectious bronchitis Coronavirus avian infectious bronchitis virus Coronavirus Replication and Interaction with Host Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism Interleukin-6 expression after infectious bronchitis virus infection in chickens Review of infectious bronchitis virus around the world Nephropathogenic avian infectious bronchitis viruses The role of phagocytic cells in enhanced susceptibility of broilers to colibacillosis after Infectious Bronchitis Virus infection Ability of Massachusetts-type infectious bronchitis virus to increase colibacillosis susceptibility in commercial broilers: a comparison between vaccine and virulent field virus Diseases of Poultry A reverse transcriptase-polymerase chain reaction survey of infectious IBV infection bronchitis virus genotypes in Western Europe from Isolation of a variant infectious bronchitis virus in Australia that further illustrates diversity among emerging strains A novel genotype of avian infectious bronchitis virus isolated in Japan in 2009 Association of the chicken MHC B haplotypes with resistance to avian coronavirus Transcriptional analysis of avian embryonic tissues following infection with avian infectious bronchitis virus Transcriptome of Local Innate and Adaptive Immunity during Early Phase of Infectious Bronchitis Viral Infection Identification of a novel coronavirus in patients with severe acute respiratory syndrome A novel coronavirus associated with severe acute respiratory syndrome Investigations into resistance of chicken lines to infection with infectious bronchitis virus Use of highly inbred chickens in research Studies on genetic resistance to Marek's disease Guide to Probe Logarithmic Intensity Error (PLIER) Estimation. Dramatic differences in gene expression of certain genes, including DDT, SRI, BLB1, HSCB, BF1, BF2, SUCLG2, MX1 and SRI, which are more highly expressed in the resistant N line Table S2 shows all 1930 DE probes)
- keywords: 15i; additional; affymetrix; analysis; array; avian; biological; birds; bronchitis; candidate; cd4; cell; chicken; control; coronavirus; data; days; differences; different; disease; dpi; early; expander; expression; fig; figure; file; functions; genes; host; host response; ibv; ibv infection; identify; immune; important; infected; infection; infectious bronchitis; innate; interferon; involved; known; levels; line; mhc; mortality; mx1; normalised; pathway; pcr; potential; processes; production; proteins; quality; resistant; respiratory; response; results; rna; role; samples; significant; strain; study; susceptible; system; table; time; trachea; viral; virus; viruses
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- cord-344745-sgkq1l93
- author: Selim, Karim
- title: Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012
- date: 2013-11-18
- words: 2765
- flesch: 50
- summary: One of a series of the Veterinary Medicine-Large Animal Clinical Sciences Department, Florida Cooperative Extension Service Avian infectious bronchitis virus Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand Severe acute respiratory syndrome vaccine development experiences of vaccination against avian infectious bronchitis coronavirus Identification of five bronchitis virus (IBV) strains isolated in China and phylogenetic analysis of the S1gene Complete genomic sequence analysis of infectious bronchitis virus Ark DPI, strain and its evolution by recombination Isolation and molecular characterization of infectious bronchitis virus isolate Shiraz IBV, by RT PCR and restriction enzyme analysis S1 gene sequence analysis of a nephro-pathogenic strain of avian infectious bronchitis virus in Egypt Studies on infectious bronchitis in broiler chickens in El-Menia Governorate Present status of infectious bronchitis in Egypt Emergence of a novel genotype of avian infectious bronchitis virus in Egypt Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates Molecular analysis of the 793/B serotype of IBV in great Britain CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods Detection of infectious bronchitis Laboratory manual for the isolation and identification of avian pathogens Molecular epizootiology of avian infectious bronchitis in Russia Isolation and molecular characterization of Sul/01/09avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East Antigenic and S-1 genomic characterization of the Delaware variant serotype of infectious bronchitis virus Spike gene analysis of the DE072 strain of infectious bronchitis virus: origin and evolution Isolation and characterization of infectious bronchitis virus strain 4/91 from commercial layer chickens in the Sudan Identification of a novel nephropathogenic infectious bronchitis virus in Israel Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey It is very critical to complete genetic characterization of circulating IBV viruses to study the genetic relatedness among viruses and vaccine strains.
- keywords: acc; analysis; avian; broiler; bronchitis; characterization; chicken; comparison; disease; egypt; egyptian; farms; gene; genetic; governorates; group; ibv; infectious; is/885; isolates; isolation; israeli; layer; molecular; pcr; positive; qiagen; region; related; respiratory; samples; selected; sequence; sp1; specific; spike; strains; study; table; vaccination; variant; virus; viruses
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- cord-345088-krb1eidw
- author: Shen, S
- title: A single amino acid mutation in the spike protein of coronavirus infectious bronchitis virus hampers its maturation and incorporation into virions at the nonpermissive temperature
- date: 2004-09-01
- words: 6978
- flesch: 47
- summary: Although S protein is dispensable for the formation of virus-like-particles (VLPs), its incorporation into virion is critical to the assembly of infectious virus particles. A portion of S protein might be transported to the plasma membrane and responsible for the cell-to-cell membrane fusion, resulting in the formation of typical syncycia and spread of virus infection to neighboring cells.
- keywords: acid; activity; amino; anti; buffer; cells; cold; coronavirus; different; domain; endo; envelope; et al; expression; fig; form; fusion; gene; glycoprotein; glycosylated; golgi; ibv; infected; insertion; kda; lanes; membrane; molecular; murine; mutant; mutation; nonpermissive; panel; particles; passages; phenotype; plaque; protein; receptor; responsible; rev-1; revertant; s protein; sequence; spike; spike protein; staining; temperature; ts mutant; ts282902; ts291602; vero; viral; virions; virus; viruses; wt6501
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- cord-345630-bam3pa70
- author: Lee, Han-Jung
- title: The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase
- date: 1991-02-28
- words: 5981
- flesch: 59
- summary: This possibility is consistent with the finding that MHV RNA synthesis occurs on the membrane fractions in the infected cells (Brayton et al., 1982) . It has been shown that there are at least five to six complementation groups involving MHV RNA synthesis, five of which have been mapped within gene 1 (Leibowitz eta/., 1982; Baric eta/., 1990) .
- keywords: 5'-end; a/.; a59; acid; amino; analysis; bredenbeek; cdna; cells; cleavage; clones; codon; comparison; complete; conserved; coronavirus; corresponding; data; diverged; domains; et al; eta/.; fig; frameshifting; functional; gene; genome; gorbalenya; hepatitis; ibv; jhm; lai; like; long; membrane; mhv; mouse; murine; nucleotide; open; orf; papain; polymerase; polyprotein; products; protease; proteins; putative; reading; region; residues; ribosomal; rna; sequence; similar; similarity; sites; specific; strain; structure; synthesis; terminal; translation; viral; viruses
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- cord-346516-lal35iyr
- author: Hughes, Laura A.
- title: Genetically Diverse Coronaviruses in Wild Bird Populations of Northern England
- date: 2009-07-17
- words: 1496
- flesch: 45
- summary: Although samples were collected from wild bird populations comprising 46 species of wild birds from numerous and diverse habitats, coronavirus RNA was detected only in wildfowl (Anseriforms) and waders (Charadriiformes). The role of wild birds in the epidemiology of IBV is poorly understood.
- keywords: analysis; anas; animal; birds; bronchitis; confidence; coronavirus; distances; diverse; evolutionary; fecal; figure; ibv; individual; infectious; method; nucleotide; pcr; phylogenetic; pooled; rna; samples; sequences; species; strain; study; table; tree; units; whooper; wild; wildfowl
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- cord-346629-770qyee8
- author: Mase, M.
- title: Phylogenetic analysis of avian infectious bronchitis virus strains isolated in Japan
- date: 2004-07-15
- words: 2367
- flesch: 46
- summary: In this study, prevalent IBV genetic groups in Japan were shown to be completely distinct from other known serotypes from Europe and North America by comparing amino acid sequences of the N-terminus in the S1 glycoprotein. Classically, IBV strains have been placed into serologically related groups on the basis of virus neutralization tests (VNT).
- keywords: amino; analysis; avian; bronchitis; china; coronavirus; countries; genetic; glycoprotein; groups; ibv; infectious; isolates; japan; japanese; major; nucleotide; outbreaks; pcr; phylogenetic; products; recent; recombination; region; restriction; sequences; serotypes; strains; study; taiwan; vaccine; virus; viruses
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- cord-348660-qnbgywgy
- author: Yilmaz, Huseyin
- title: Production of Recombinant N Protein of Infectious Bronchitis Virus Using the Baculovirus Expression System and Its Assessment as a Diagnostic Antigen
- date: 2018-07-09
- words: 3928
- flesch: 38
- summary: Recombinant IBV N protein was expressed using passage 2 or higher passage recombinant baculovirus stocks (> 10 7 pfu/ml). Approximately, 100 μl of sample containing approximately 5 μg of recombinant IBV N protein was diluted in 50-μl Laemmli sample dilution buffer (Santa Cruz Biotechnology, Dallas, TX) and resolved in 12% Bis-Tris polyacrylamide gel as described above.
- keywords: antibodies; antibody; antigen; antisera; avian; baculovirus; bronchitis; cat; chickens; chicks; commercial; control; detection; diagnostic; dilution; elisa; expression; fig; gene; house; ibv; ibv n; immunized; indirect; infected; infectious; life; local; monoclonal; n protein; negative; performance; plasmid; positive; potential; protein; reactivity; recombinant; recombinant ibv; samples; sera; serum; specific; strain; study; system; technologies; test; turkey; vaccinated; value; virus
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- cord-349149-nqsohp9h
- author: Lounas, A.
- title: The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria
- date: 2018-11-29
- words: 3702
- flesch: 47
- summary: Infectious bronchitis An apparently new respiratory disease of baby chicks A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China Etiology of an infectious nephritis-nephrosis syndrome of chickens Avian infectious bronchitis: Characterization of new isolates from Italy Isolation and identification of four isolates of infectious bronchitis strains in China and analysis of their SI protein gene Isolation of 4/91 type of infectious bronchitis virus as a new variant in Japan and efficacy of vaccination against 4/91 type field isolate An overview of infectious bronchitis virus in chickens Phylogenetic analysis of avian infectious bronchitis virus S1 glycoprotein regions reveals emergence of a new genotype in Moroccan broiler chicken flocks S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during A reverse transcriptase polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from Molecular epizootiology of avian infectious bronchitis virus in Russia Coronavirus avian infectious bronchitis virus Evidence of natural recombination within the S1 gene of infectious bronchitis virus Gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel Characterization and analysis of an infectious bronchitis virus strain isolated from southern China in 2013 Genetic grouping of nephropathogenic avian infectious bronchitis virus isolated in Morocco Virulent avian infectious bronchitis virus, people's republic of China ) Vet World DOI: 10.14202/vetworld.2018.1630-1636 sha: doc_id: 349149 cord_uid: nqsohp9h BACKGROUND AND AIM: Avian infectious bronchitis virus (IBV) frequently infects broilers and is responsible for severe economic losses to the poultry industry worldwide.
- keywords: 4/91; acid; algeria; amino; analysis; avian; broiler; broiler flocks; bronchitis; bronchitis virus; chickens; china; cross; damage; different; farms; field; flocks; gene; genotype; high; ibdz13a; ibv; infectious; infectious bronchitis; inhibition; isolates; isolation; kidney; min; nephritis; nephropathogenic; new; pcr; poultry; protection; reaction; results; reverse; samples; sequences; serotypes; strain; study; test; vaccinated; vaccination; vaccine; variant; virus
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- cord-351564-nikcd44o
- author: Zhang, Xiaozhan
- title: Molecular characterization of variant infectious bronchitis virus in China, 2019: Implications for control programmes
- date: 2020-01-24
- words: 2808
- flesch: 34
- summary: Recombination events among IBV strains were further investigated using the RDP4 software, a widely used tool for analysing individual recombination events and overall recombination patterns, by seven different algorithms recombination detection program (RDP), Bootscan, MaxChi, GENECONV, Chimaera, SiScan and 3Seq (Martin, Murrell, Khoosal, & Muhire, 2017 ). As shown in Figure S1a To further characterize the biology and ecology of the newly identified IBV strains, we sequenced the complete S1 genes of Phylogenetic relationship among IBV strains has been established on the analysis of complete S1 gene.
- keywords: amino; analysis; available; avian; bande; broilers; bronchitis; bronchitis virus; characterization; china; china/2019; commercial; complete; control; coronavirus; day; disease; et al; events; figure; genes; genotype; hen-2; hvr; ibv; ibv strains; infectious; infectious bronchitis; like; mutations; novel; number; old; outbreaks; phylogenetic; poultry; protein; recombination; region; respiratory; severe; software; strains; structure; study; vaccinated; vaccine; virus
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- cord-352511-gkm7i62s
- author: Yamada, Yoshiyuki
- title: Acquisition of Cell–Cell Fusion Activity by Amino Acid Substitutions in Spike Protein Determines the Infectivity of a Coronavirus in Cultured Cells
- date: 2009-07-02
- words: 5816
- flesch: 49
- summary: The molecular biology of coronaviruses The biology and pathogenesis of coronavirus Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China Bats are natural reservoirs of SARS-like coronaviruses Murine encephalitis caused by HCoV-OC43, a human coronavirus with broad species specificity, is partly immune-mediated Genetic evolution and tropism of transmissible gastroenteritis coronaviruses Circulation of genetically distinct contemporary human coronavirus OC43 strains Replication and morphogenesis of avian coronavirus in Vero cells and their inhibition by momensin Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41 Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cells Emergence of an avian coronavirus infectious bronchitis virus (IBV) mutant with a truncated 3b gene: functional characterization of the 3b gene in pathogenesis and replication Single Amino Acid Mutation in the Spike Protein of Coronavirus Infectious Bronchitis Virus Hampers Its Maturation and Incorporation into Virions at the Nonpermissive Temperature In vitro assembled, recombinant infectious bronchitis viruses demonstrate that the 5a open reading frame is not essential for replication Induction of p53-independent cell cycle arrest at S-and G 2 /M-phase in cells infected with the coronavirus infectious bronchitis virus promotes viral replication Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus An Arginine-to-Proline Mutation in a Domain with Undefined Functions within the Helicase Protein (NSP13) is Lethal to the Coronavirus Infectious Bronchitis Virus in Cultured Cells Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the replication and infectivity of coronavirus in cultured cells Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage site Membrane association and dimerization of a cysteinerich, 16-kDa polypeptide released from the C-terminal region of the coronavirus infectious bronchitis virus 1a polyprotein Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I A 12-amino acid stretch in the hypervariable region of the spike protein S1 subunit is critical for cell fusion activity of mouse hepatitis virus Furthermore, a G405-D point mutation in the S1 domain, which was acquired during further propagation of Vero-adapted IBV in Vero cells, could enhance the cell–cell fusion activity of the protein.
- keywords: acid; acquisition; activities; activity; adaptation; adapted; amino; animal; anti; avian; bronchitis; cell; cell fusion; chicken; clones; constructs; coronavirus; cultured; ep3; expression; f857; fig; formation; fusion; fusion activity; fusogenic; g405; gene; host; human; ibv; induction; infectious; infectivity; l857; length; membrane; murine; mutant; mutation; p65; panel; passages; process; protein; receptor; region; residue; s protein; s(ck; s(ep3; s(p65; sars; species; spike; strains; substitution; syncytia; transmission; vero; viral; virus; viruses
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- cord-353027-bc0un6kb
- author: Ali, Ahmed
- title: Safety and efficacy of attenuated classic and variant 2 infectious bronchitis virus candidate vaccines
- date: 2018-08-06
- words: 4157
- flesch: 48
- summary: Emergence of a novel genotype of avian infectious bronchitis virus in Egypt S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt Complete genome sequences of two avian infectious bronchitis viruses isolated in Egypt: evidence for genetic drift and genetic recombination in the circulating viruses Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses Progress and challenges toward the development of vaccines against avian infectious bronchitis Protection of chickens vaccinated with combinations of commercial live infectious bronchitis vaccines containing Massachusetts, Dutch and QX-like serotypes against challenge with virulent infectious bronchitis viruses 793B and IS/1494/06 Israel variant 2 A nomenclature for avian coronavirus isolates and the question of species status Coronaviruses in poultry and other birds Coronavirus avian infectious bronchitis virus The long view: 40 years of infectious bronchitis research Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Avian infectious bronchitis The early history of infectious bronchitis Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China Attenuation of avian infectious bronchitis virus by cold-adaptation Coinfection of avian influenza virus (H9N2 subtype) with infectious bronchitis live vaccine Experimental co-infection of infectious bronchitis and low pathogenic avian influenza H9N2 viruses in commercial broiler chickens Prevalence of avian respiratory viruses in broiler flocks in Egypt Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains Attenuation mechanism of virulent infectious bronchitis virus strain with QX genotype by continuous passage in chicken embryos Attenuation, safety, and efficacy of an infectious bronchitis virus GA98 serotype vaccine Evaluating protection against infectious bronchitis virus by clinical signs, ciliostasis, challenge virus detection, and histopathology Cross-protective immune responses elicited by a Korean variant of infectious bronchitis virus Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain Identification of a novel nephropathogenic infectious bronchitis virus in Israel A simple method of estimating fifty per cent endpoints Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012 Co-infection with multiple respiratory pathogens contributes to increased mortality rates in algerian poultry flocks Cross-protection by infectious bronchitis viruses under controlled experimental conditions In all experiments, white leghorn SPF chickens were used to determine the safety and efficacy of attenuated viruses.
- keywords: attenuated; avian; birds; bronchitis; challenge; chickens; chicks; ciliostasis; classic; clinical; control; day; dpi; east; efficacy; egg; eggs; egypt; egyptian; eid; et al; groups; homologous; ib2; ibm41; ibv; ibvar2; infectious; inoculated; live; middle; nephropathogenic; ocular; old; passages; protection; respiratory; route; safety; score; shedding; signs; spf; strains; studies; titers; tracheal; vaccinated; vaccines; variant; viruses
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- cord-353310-19kzb6ag
- author: Quinteros, José A.
- title: Analysis of the complete genomic sequences of two virus subpopulations of the Australian infectious bronchitis virus vaccine VicS
- date: 2015-04-01
- words: 5209
- flesch: 43
- summary: Liu & Brown, 1995; Tibbles et al., 1996; Liu et al., 1998; Ziebuhr et al., 2001; Ziebuhr, 2005) . In Australia, three genotypic subgroups of IBV have been distinguished (Hewson et al., 2012) .
- keywords: 1ab; 3cl; acid; amino; analysis; australian; avian; beaudette; bronchitis; bronchitis virus; cavanagh; china; chinese; cluster; complete; coronavirus; del; depth; determined; differences; distinct; domain; end; et al; figure; fold; gan; genes; genome; gly; glycoprotein; hewson; hewson et; ibv; infectious; infectious bronchitis; like; liu; nsp6; nucleotide; orfs; pathogenicity; phylogenetic; plp; protein; reads; recombination; region; replication; rna; sequences; sequencing; strains; structural; study; subpopulations; transcription; tree; usa; utr; vaccine; vics; viral; virus; viruses; vivo; ziebuhr
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- cord-354547-eomm1sl5
- author: Wang, Jibin
- title: Interaction of the Coronavirus Infectious Bronchitis Virus Membrane Protein with β-Actin and Its Implication in Virion Assembly and Budding
- date: 2009-03-16
- words: 6349
- flesch: 47
- summary: IBV M protein was transiently expressed in H1299 cells grown on 4-well chamber slides (IWAKI). When expressed alone, M protein accumulates in the Golgi complex in the form of homomultimeric complexes [8, 9] .
- keywords: a159; acids; actin; amino; analysis; anti; antibodies; assembly; binding; blot; bronchitis; budding; buffer; cdna; cells; constructs; coronavirus; cycle; cytochalasin; deletion; detection; envelope; essential; expression; fig; filaments; fractions; golgi; hours; ibv; infected; infectious; interaction; k160; lanes; length; m protein; md5; membrane; mm1; mutant; mutation; myc; nucleocapsid; panel; particles; pcr; post; protein; purified; region; release; replication; rna; sequence; similar; structural; study; subgenomic; transcription; transcripts; transfected; type; viral; virion; virus; viruses; western; wild; yeast
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- cord-355703-l9l4ybfn
- author: Zhao, Fei
- title: Genomic Characteristics and Changes of Avian Infectious Bronchitis Virus Strain CK/CH/LDL/97I after Serial Passages in Chicken Embryos
- date: 2014-08-29
- words: 5903
- flesch: 42
- summary: The hydrophobic domain of infectious bronchitis virus E protein alters the host secretory pathway and is important for release of infectious virus The transmembrane oligomers of coronavirus protein E The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function Analysis of constructed E gene mutants of mouse hepatitis virus confirms a pivotal role for E protein in coronavirus assembly The present of viral subpopulations in an infectious bronchitis virus vaccine with differing pathogenicity -a preliminary study Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI NMR structure of the SARS-CoV nonstructural protein 7 in solution at pH 6.5 Inhibition of protein kinase R activation and upregulation of GADD34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells The SARS-coronavirus PLnc domain of nsp3 as a replication/ transcription scaffolding protein Mouse hepatitis virus liver pathology is dependent on ADP-ribose-1 ′ ′ -phosphatase, a viral function conserved in the alpha-like supergroup Evidence for a pseudoknot in the 3 ′ untranslated regions of the bovine coronavirus genome Avian coronavirus infectious bronchitis attenuated live vaccines undergo selection of subpopulations and mutations following vaccination Host intraspatial selection of infectious bronchitis virus Effects of infectious bronchitis on the reproductive tracts, egg production, and egg quality of laying chickens Pathogenesis of oviduct lesions in immature chickens following exposure to infectious bronchitis virus at one day old Pathogenesis of two embryo passage levels of avian infectious bronchitis virus for the oviduct of young chickens of various ages Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cells Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation Two amino acid changes at the N-terminus of transmissible gastroenteritis coronavirus spike protein result in the loss of enteric tropism In addition, accurate estimation and comparison of the nucleotide sequences of the entire genomes of IBV CK/CH/LDL/97I P5 and P115 were conducted using the ClustalW algorithm (www. clustal.org).
- keywords: acid; amino; analysis; arkansas; attenuated; attenuation; avian; bronchitis; changes; chickens; china; coronavirus; covs; different; domain; dpi; eggs; embryo; entire; functions; gene; genetic; genome; genomic; host; ibv; ibvs; immunogenicity; infectious; ldl/97i; ldl/97i p5; like; live; loss; lx4; mass; mutations; nsp3; nucleotide; orf; oviduct; p115; parental; passage; pathogenic; pathogenicity; pcr; potential; products; protein; region; replicase; replication; rna; sequence; serial; similar; spike; strain; structural; study; subpopulations; substitutions; tail; tissue; tropism; type; type(china; vaccine; variations; viral; virulence; virus; viruses
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- cord-356094-sbtigcfr
- author: Chen, Huijie
- title: Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
- date: 2019-10-29
- words: 8929
- flesch: 42
- summary: The specific operations were as follows: CEK cells were cultured in 96-well tissue culture plates, and when the cells were full of monolayer, IBV virus was diluted to 10 −1 , 10 −2 , 10 −3 …, and 10 −12 times with serum-free M199, respectively. This further confirmed that HPE has a very good inhibitory effect on IBV infected cells.
- keywords: acetate; acid; activity; addition; alpha; antiviral; avian; beta; bronchitis; bronchitis virus; cek; cek cells; cells; chickens; chicks; compounds; concentration; control; cpe; culture; day; drug; effect; et al; ethyl; expression; expression levels; extract; factor; figure; gene; group; hpe; hpw; hypericum; hyperoside; ibv; ibv infection; ifn; il-6; immune; increase; infected; infection; inflammatory; influenza; interferon; kidney; layer; levels; m41; mavs; mda5; method; min; molecular; mrna; mrna expression; order; pathway; pcr; perforatum; protein; pseudohypericin; qrt; quercetin; reaction; relative; renal; results; reverse; ribavirin; signaling; significant; solution; spf; standard; study; tcid; time; titer; tnf; total; trachea; treatment; type; virus; vitro; vivo; volume; water; zhang
- versions: original; plain text