key: cord-333368-kjrk8nn9
authors: Huizinga, Gabrielle P; Singer, Benjamin H; Singer, Kanakadurga
title: The Collision of Meta-Inflammation and SARS-CoV-2 Pandemic Infection
date: 2020-09-03
journal: Endocrinology
DOI: 10.1210/endocr/bqaa154
sha: 
doc_id: 333368
cord_uid: kjrk8nn9

The COVID-19 pandemic has forced us to consider the physiologic role of obesity in the response to infectious disease. There are significant disparities in morbidity and mortality by sex, weight and diabetes status. Numerous endocrine changes might drive these varied responses to SARS-CoV-2 infection including hormone and immune mediators, hyperglycemia, leukocyte responses, cytokine secretion, and tissue dysfunction. Studies of patients with severe COVID-19 disease have revealed the importance of innate immune responses in driving immunopathology and tissue injury. In this review we will describe the impact of the metabolically induced inflammation (meta-inflammation) that characterizes obesity on innate immunity. We consider that obesity-driven dysregulation of innate immune responses may drive organ injury in development of severe COVID-19 and impair viral clearance.

The chronic obesity-induced inflammation characterized by increased tissue and circulating myeloid cells has been termed metabolic inflammation or meta-inflammation. The coronavirus disease 2019 pandemic has highlighted the endocrine manifestations of obesity and impact of diabetes and obesity on immune responses to infectious disease. Obesity and metabolic syndrome have been identified as risk factors for severe manifestations of SARS-CoV-2 infection 1, 2 . Prior studies of the epidemiology of sepsis, the acute respiratory distress syndrome (ARDS), and other acute illnesses have raised the question of whether obesity is associated with lower mortality in critical illness -the "obesity paradox" 3, 4 . However, the COVID-19 pandemic has forced reconsideration of the impact of obesity and diabetes on disease outcomes. While obesity and diabetes may complicate the delivery of supportive care in critical illness regardless of the underlying disease, lessons learned from the interaction of obesity with other systemic inflammatory syndromes suggest that obesity modifies biologic factors related to SARS-CoV-2 infection and the COVID-19 syndrome.

COVID-19 was first reported in Wuhan, China in December 2019 and first made its appearance in the United States in Washington State on January 31, 2020. Outbreaks quickly spread throughout Washington and California before spreading to the rest of the US. Obesity was not studied in the early SARS-CoV-2 studies coming from Wuhan, China, likely because so little of the population is obese. However, pre-existing type 2 diabetes was demonstrated to be a risk factor for illness severity 5 . In the US, obesity, hypertension, and diabetes are commonly reported among the most common comorbidities for patients hospitalized with COVID-19 across several metropolitan disease outbreaks 2,6-8 . When considering the obesity epidemic in the US, this is not surprising, as approximately 35% of men and 40% of women in the United States are obese by body mass index (BMI > 30 kg/m2) 9, 10 . However, obesity is independently associated with odds of hospital or intensive care admission among patients presenting for medical care 1, 2 . Several studies suggest that A c c e p t e d M a n u s c r i p t 4 obesity is an independent predictor of hypoxic respiratory failure 11, 12 and death 13,14 among hospitalized patients, even among young patients with fewer comorbidities 15, 16 . Decisions about hospital admission, and thus the characteristics of hospitalized patients, may be confounded by the expectation that obese patients require closer monitoring. Population-based estimates of COVID-19 mortality, however, also show a 2.6-fold increase in risk for patients with BMI > 40 17 .

Prior meta-analyses of both ARDS and sepsis have found either no harm or reduction in mortality among obese adults 18, 19 . However, severity of illness and organ damage has been demonstrated to increase based on obesity in pediatric patients [20] [21] [22] [23] . While changes in critical care practice during a pandemic could explain differences in mortality or ICU admission, the association of obesity with physiologically defined hypoxic respiratory failure suggests a biologic interaction of obesity with SARS-CoV-2 infection. Obesity as a risk factor for severe infectious disease is not a new concept and is not limited to coronavirus infections. During the 2009 influenza A/H1N1 pandemic, it was noted that obese patients were more likely to experience more severe disease requiring hospitalization than normal weight patients 24 . Additionally, even when both groups were vaccinated, obese individuals were more likely to become infected with the influenza virus 25 . Along with viral infections, obesity alters the course of bacterial infections 26 . All of this clinical evidence emphasizes the importance of further understanding the mechanisms by which obesity influences immune responses.

The current SARS-CoV-2 pandemic has highlighted the increased need for research to understand the mechanisms behind severity of pandemic infection in different risk groups.

A c c e p t e d M a n u s c r i p t 5 With the prevalence of obesity rising without an end in sight, it is important to understand the endocrine, metabolic, and inflammatory shifts that occur in obesity may drive increased pathogendriven morbidity and mortality. A c c e p t e d M a n u s c r i p t 6 Pro-inflammatory circulating monocyte and macrophage populations are associated with a number of chronic diseases, including obesity 32 . A striking feature of obesity in both clinical and animal studies is the chronic low-grade inflammation tied with metabolic diseases, meta-inflammation 33 .

Increased BMI correlates with an increase of several cytokines including IL-6, IL-8, IL-1, and TNF- and obesity also correlates with an increase in chemokines such as CCL14 34, 35 and MCP-1 36 furthering the overall inflammatory tone.

Hyperglycemia alone may lead to significant changes in macrophage function and inflammation. In patients with diabetes, increased glucose levels and glycolysis promoted Sars-CoV-2 replication in monocytes via ROS/HIF pathway activation leading to secondary T-cell dysfunction 37 . Patients with well controlled blood glucose levels were less likely to experience serious complications and death from COVID-19 compared to diabetic patients with poorly controlled blood glucose levels. The wellcontrolled patients had lower IL-6, C Reactive Protein, and LDH levels, as well as only a 1.1% mortality rate, which is significantly lower than the 11% mortality rate seen in the poorly controlled group 5 . This association of diabetes diagnosis with COVID-19 mortality is also observed on the population level as well 17 . While one cannot directly say the hyperglycemia causes this enhanced mortality, there is abundant evidence that obesity leads to long-term reprogramming of the innate immune system.

Macrophages from obese animals and humans have been described as metabolically active, M1 polarized, and pro-inflammatory with both regulatory and detrimental activity [38] [39] [40] . These macrophages produce cytokines, chemokines, reactive oxygen species, and factors regulating fibrosis and metabolism. Overall, our understanding is that these metabolic macrophages have a similar profile to those stimulated with lipopolysaccharide (LPS), an abundant bacterial derived A c c e p t e d M a n u s c r i p t 7 molecular pattern molecule and ligand of the Toll-like receptor 4 (TLR4). More recently it has become clear that while the inflammatory phenotype of these macrophages is closest to what is seen with LPS stimulation, traditional M1 macrophages, the added activation by fatty acids creates a unique phenotype that has characterized these obesity myeloid cells as metabolically active macrophages 41 .

While changes to macrophage phenotype in obesity were originally characterized in the adipose tissue 36, 42 , it is now evident that obesity has significant effects on hematopoiesis, circulating monocytes, and macrophages in multiple organs. Elevated BMI and obesity has been shown to enhance hematopoiesis and expand myeloid cell production 43,44 but it has also been shown to impair immune responses in a TLR4 dependent manner 45 . High fat diet increases the number of monocytes in circulation and expands bone marrow macrophages, neutrophils and their progenitors 43 . Expansion of these progenitors leads to increased macrophage production, but also skews the resulting macrophage population to a pro-inflammatory phenotype 43, 44, 46 .

During obesity there is an overall increase in chemokines, which play a role in metabolic inflammation by recruiting monocytes into adipose tissue. Additionally, during obesity and type 2 diabetes (T2D), there is an increase in circulation of free fatty acids (FFAs), including palmitate (PA).

PA induces CCL4 release from monocytes and macrophages by interacting with TLR4 47 . Obese individuals, regardless of diabetes status, also have higher circulating levels of LPS 48,49 which binds to TLR4. This binding increases the production of the chemokine CCL2 in monocytes and macrophages 50 . This further leads to enhanced tissue macrophages that have the potential to lead to dysfunctional cytokine production and tissue damage if triggered.

A c c e p t e d M a n u s c r i p t 8 While the phenotype of these macrophages in driving impaired metabolism is well described there are several other implications of these metabolically activated macrophages 51 . In pulmonary viral infections such as influenza, macrophages from obese mice exhibit enhanced and likely injurious pro-inflammatory cytokine production but impaired production of antiviral type-I interferons 52, 53 .

Obese mice suffer increased interstitial inflammation, alveolar permeability, and lung injury even in the absence of increased viral load 54 . Chronic systemic inflammation is accompanied by impaired induction of pathogen-induced and lung-specific responses to influenza across a variety of obesity models 55 . Pro-inflammatory activation of macrophages in obesity impairs their function in other domains, as well. For example, in obese diabetic mice, macrophages recruited to diabetic wounds as a result of epigenetic alterations have a pro-inflammatory phenotype and have elevated levels of prostaglandin E 2 (PGE 2 ) production [56] [57] [58] [59] [60] . PGE 2 signaling can impair macrophage innate immune functions as well as alter production of pro-inflammatory cytokines 61 . This activated state causes delayed wound healing but may also have further implications in responses to infection, which is a major physiologic function of macrophages. PGE 2 signaling instructs macrophages to secrete IL-10 and influences naïve T cells to shift from a Th1 to a Th2 phenotype. This Th2 phenotype causes a decreased ability to clear intracellular pathogens, such as viruses 62 .

Immune system activation in obesity is not confined to adipose tissue or organ dysfunction related to metabolic disease, such as the liver or vasculature, but also has a negative effect on the immune system on the whole, leading to an increase risk of infection 63 . It is well recognized clinically that diabetes negatively impacts the body's response to infection. Hyperglycemia stemming from T2D caused by obesity has proven to reduce control of invading pathogens 64 Even more broadly in sepsis models, obese animals have been seen to have more severe organ damage and worsened survival 76,77 . A c c e p t e d M a n u s c r i p t 10 Even a short-term high fat diet can impact the reaction to a bacterial infection. Mice fed a high fat diet for 16 days and then orally challenged with Listeria monocytogenes had reduced inflammatory responses, and as a result increased bacterial load and increased numbers of goblet cells 78 .

Obese individuals are not only susceptible to severe bacterial infections, but also severe viral infections . Obesity has been shown to be a risk factor for human papilloma virus incident infection, however obesity was not associated with how long the infection persisted 79 . Additionally, in the 2009 H1N1 Influenza pandemic, obesity was a major risk factor for severe infection and death 24 .

Adults aged 20 years or older who died from H1N1 infection were more likely to be obese or morbidly obese 80 . When lean and obese mice are infected with H1N1, although the lungs exhibit the same viral titer, the obese mice lost more weight and experienced more pulmonary pathology than lean mice. Additionally, the virus spread to the alveolar epithelial cells in the obese mice. The increased spreading of the virus in obese mice combined with the reduction in local production of several pro-inflammatory cytokines (while still increased in circulation) likely contributed to the increased murine morbidity and mortality due to infection 81 . The same illness severity and poor responsiveness to treatment with obesity has been demonstrated in seasonal influenza infections 82 .

It has been widely speculated that higher ACE-2 expression in adipose tissue may result in higher total body SARS-CoV-2 viral load in obese individuals. Early reports have not demonstrated a correlation among viral load and obesity or initial viral load and disease severity 83 . In seasonal and pandemic influenza, however, obese individuals may be more susceptible to severe viral respiratory disease even if they mount a serologic response to vaccination 25 A c c e p t e d M a n u s c r i p t 11 Along with possible impairments in pathogen clearance, obese hosts are more likely to experience the breakdown of respiratory epithelium during a pulmonary infection, which leads to increased fluid in the airway space. This allows the pathogen to have the opportunity to more easily spread throughout the body and leaves the host with reduced lung function 63 .

The SARS-CoV-2 virus, like other members of the betacoronavirus subfamily 85 , enters mammalian cells through the interaction of the viral envelope spike glycoprotein and angiotensin converting enzyme 2 (ACE-2) on host cells 86 . ACE-2 is expressed in many human tissues, including not only the lungs but also kidney, brain, adipose tissue and small intestine, raising the question of which symptoms of COVID-19 are due to direct viral effects versus systemic immune responses, especially in severe disease 87 . Tropism of SARS-CoV-2 for extrapulmonary tissues is confirmed by detection of viral RNA from samples outside the respiratory tract 88 . ACE-2 is upregulated in adipocytes in obese and diabetic patients, which allows the virus to target and replicate in adipose tissue and has led to speculation that adipose tissue can serve as a reservoir of SARS-CoV-2, potentially worsening disease severity in obese individuals [89] [90] [91] . A correlation between respiratory tract viral load and obesity, however, has not been confirmed 83 .

Intestinal involvement in SARS-CoV-2 infection may interact with obesity associated metainflammation. While meta-inflammation in obesity is a systemic, multifactorial process 92 , the gut microbiome is known to have a bidirectional relationship to meta-inflammation 93 influencing intestinal inflammation 94 . SARS-CoV-2 infection has been associated with shifts in gut microbiota to more pathogenic taxa, as have other states of critical illness 95 . These shifts may encourage a state of A c c e p t e d M a n u s c r i p t 12 systemic inflammation. Both dysbiosis and the direct enteropathic effect of SARS-CoV-2 infection may promote gut barrier permeability and increase metabolic endotoxemia, a potential mediator of metabolic disease and meta-inflammation in obesity 96 . Establishing a connection between gut dysfunction and meta-inflammation in COVID-19 survivors will require long-term studies.

While considerable speculation has focused on how ACE-2 levels are driven by either polymorphisms, comorbidity, or environmental factors, other genetic factors may also play a role in susceptibility to severe COVID-19 disease, as well 97 . The first genome-wide association study of COVID-19 severity identified two loci -one containing the ABO blood group locus and another at 3p21.31 98 . The latter locus contains several chemokine genes, the expression of which may plausibly be altered in meta-inflammation. An ongoing multinational effort continues to examine how host genetics may inform susceptibility to severe COVID19 and may reveal factors that interact with gene expression obesity 99 . While genetic factors explain a small part of the risk for developing diabetes, diabetes risk genes typically do not include those related to antiviral immunity 100 , emphasizing that susceptibility to SARS-CoV-2 infection or development of severe COVID19 disease is likely due to environmental exposure and pathophysiology that develops through the life course, rather than a common predisposing genotype.

In addition to obesity and comorbidity, male sex confers a significantly increased risk of severe COVID-19 disease and death 14 . Differences in myeloid inflammation among males and females may play a protective role in the immunopathology of COVID-19, especially in the setting of obesity.

A c c e p t e d M a n u s c r i p t 13 Not all obese individuals are at risk for metabolic and cardiovascular disease 102 and not all obese develop obesity induced inflammation to the same degree 103 . Increased androgen concentration in males is also thought to lead to increased IL-10 production when peripheral blood mononuclear cells are stimulated by viral antigens, leading to a delayed and diminished pro-inflammatory response which may also explain disease severity 104 . Additionally, males tend to have increased levels of proinflammatory cytokines and chemokines after stimulation with LPS intraperitoneally in vivo and in in vitro assays 105, 106 . Hence, this difference by sex and due to sex hormones could lead to a possible increased cytokine storm in males with obesity 107 , and explain the pathologic response and enhanced morbidity described clinically.

Pre-menopausal females are relatively protected from obesity induced inflammation, macrophage activation, and expansion of myeloid progenitors and are relatively protected from metabolic and cardiovascular disease 108 . In pre-clinical models, females are protected from meta-inflammation [108] [109] [110] [111] . One explanation for this is that females generally handle the expansion of adipocytes with resident macrophage proliferation without recruitment of pro-inflammatory macrophages 109 . This finding that all macrophages are not the same in responsiveness to obesity but that the overall trend is pro-inflammatory in nature requires further investigation into the overall macrophage phenotype with obesity. In the context of infection though, those with pro-inflammatory macrophages can produce an enhanced cytokine environment leading to severity of illness in obese individuals via both systemic and local lung effects 112, 113 .

While the interaction of sex and diet induced obesity has been most elucidated in the setting of innate immune cell populations, sex hormone receptors are present on cells of the adaptive immune system as well, and sexual dimorphism in autoimmunity and humoral responses has been noted for A c c e p t e d M a n u s c r i p t 14 decades 114 . These differences may also be critical in favoring antiviral immunity in females. Of particular note, female mice expand the population of regulatory T-cells significantly compared to male mice in obesity 115 . This cell population is noted to be deficient in obese male mice during influenza infection 54 and may play a role in limiting lung injury in the setting of viral infection.

Individuals of all ages are at risk for obesity, but it is not clear if weight status influences what has been seen with age related risk for COVID severity. Of patients aged 19-64 years admitted to the hospital, those with co-morbidities caused by high BMI were more likely to be admitted to the ICU 63 and this is true regardless of sex 10 . As humans age, the immune system changes 116 , with a decreased number of lymphocytes, which are important cells for fighting viruses. This has proved to be a significant disadvantage during past epidemics, such as SARS 117 . While elderly individuals are at increased risk for severe COVID-19, the obesity epidemic is shifting to the most targeted demographic to a younger age group. Younger COVID patients are likely to have a higher BMI then older patients while this same trend did not exist in non-COVID admissions 118 . Age related changes in metabolic inflammation are still being understood regardless of infection 119 and the impact of this on COVID-19 needs to be further examined.

On the other extreme, young children have fared better than adults during this pandemic. Many viruses, including respiratory syncytial virus and influenza virus, infect children at particularly high rates compared to adults aged 30-65. However, children appear to be less susceptible to SARS, MERS, and SARS-CoV-2 infection 120 . Additionally, few children present with common co-morbidities seen in adults, such as hypertension, T2D, cancers, and pulmonary diseases. The absence of these diseases may allow children to only have a mild case of the virus 122 . More recently some children A c c e p t e d M a n u s c r i p t 15 who have recovered from SARS-CoV-2 infection are presenting with post-infectious cytokine release syndrome, indicative by a fever, GI symptoms, and a rash. Consistent with early life metainflammation with obesity it appears that obese children are at higher risk for severe disease 123 .

Does hyperglycemia worsen COVID-19 or does COVID-19 lead to immune pancreatic damage leading to hyperglycemia?

COVID-19 patients have been found to have elevated blood sugars during hospitalization and those with uncontrolled diabetes had a higher mortality 124 . However, SARS-CoV-2 virus can also damage the pancreas by infecting the  and  cells, which function to regulate blood sugar levels. In severe COVID-19 cases, 17% of patients were diagnosed with pancreatic injury, compared to only 1-2% in non-severe cases 125 . One possible explanation is that the virus can also increase pro-inflammatory cytokines that destroy these cells. This could possibly lead to an autoimmune disease and development of type 1 diabetes (T1D) 126 . Prior studies have demonstrated that SARS coronavirus itself can directly damage islets 127 , further research will be needed to see if T1D or beta cell failures were caused by COVID-19 and if this has happened to other individuals 128 . Given ACE-2 expression in the pancreas it has been hypothesized that this may be a mechanism for pancreatic damage specifically in SARS-Cov2 infection 127 .

Obesity is a significant risk factor for severe COVID-19 illness. Patients with obesity and metabolic disease frequently experience a state of low-grade chronic meta-inflammation. Prior studies of vaccine efficacy and response to viral and bacterial infection show that meta-inflammation may significantly alter the response to pathogens. While this has not been directly established in SARS-CoV-2 infection, emerging evidence indicates that disordered myeloid inflammatory responses are a A c c e p t e d M a n u s c r i p t

Clinical Characteristics and Morbidity Associated With Coronavirus Disease 2019 in a Series of Patients in Metropolitan Detroit

Characteristics Associated with Hospitalization Among Patients with COVID-19

Overweight or obese BMI is associated with earlier, but not later survival after common acute illnesses

Obesity and 1-year outcomes in older Americans with severe sepsis

Association of Blood Glucose Control and Outcomes in Patients with COVID-19 and Pre-existing Type 2 Diabetes

Clinical Characteristics of Covid-19 in New York City

Epidemiology, clinical course, and outcomes of critically ill adults with COVID-19 in New York City: a prospective cohort study

Presenting Characteristics, Comorbidities, and Outcomes Among 5700 Patients Hospitalized With COVID-19 in the New York City Area

The obesity transition: stages of the global epidemic

Obesity could shift severe COVID-19 disease to younger ages

Obesity and COVID-19 in New York City: A Retrospective Cohort Study

Obesity is associated with worse outcomes in COVID-19: Analysis of Early Data From

Obesity is Associated with Increased Risk for Mortality Among Hospitalized Patients with COVID-19

Severe obesity, increasing age and male sex are independently associated with worse in-hospital outcomes, and higher in-hospital mortality

Obesity as a Potential Predictor of Disease Severity in Young COVID-19 Patients: A Retrospective Study

Morbid Obesity as an Independent Risk Factor for COVID-19 Mortality in Hospitalized Patients Younger than 50

OpenSAFELY: factors associated with COVID-19 death in 17 million patients

Can body mass index predict clinical outcomes for patients with acute lung injury/acute respiratory distress syndrome? A meta-analysis

The role of increased body mass index in outcomes of sepsis: a systematic review and meta-analysis

Body Habitus and Risk of Mortality in Pediatric Sepsis and Septic Shock: A Retrospective Cohort Study

Outcomes and Resource Use Among Overweight and Obese Children With Sepsis in the Pediatric Intensive Care Unit

Influence of Obesity Diagnosis With Organ Dysfunction, Mortality, and Resource Use Among Children Hospitalized With Infection in the United States

The Role of Obesity in Sepsis Outcome among Critically Ill Patients: A Retrospective Cohort Analysis

Underweight, overweight, and obesity as independent risk factors for hospitalization in adults and children from influenza and other respiratory viruses

Increased risk of influenza among vaccinated adults who are obese

Impact of obesity on sepsis mortality: A systematic review

Cytokine release syndrome in severe COVID-19

Predicting severe outcomes in COVID-19

A single-cell atlas of the peripheral immune response in patients with severe COVID-19

Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

Pathological inflammation in patients with COVID-19: a key role for monocytes and macrophages

Perturbation of the Monocyte Compartment in Human Obesity

Inflammatory links between obesity and metabolic disease

Immune dysfunction in patients with diabetes mellitus (DM)

Cytokine, Chemokine, and Cytokine Receptor Changes Are Associated With Metabolic Improvements After Bariatric Surgery

CCR2 modulates inflammatory and metabolic effects of high-fat feeding

Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1alpha/Glycolysis-Dependent Axis

Metabolically Activated Adipose Tissue Macrophages Perform Detrimental and Beneficial Functions during Diet-Induced Obesity

Unique metabolic activation of adipose tissue macrophages in obesity promotes inflammatory responses

Metabolic dysfunction drives a mechanistically distinct proinflammatory phenotype in adipose tissue macrophages

Adipocyte-macrophage interaction may mediate LPS-induced lowgrade inflammation: potential link with metabolic complications

Obesity induces a phenotypic switch in adipose tissue macrophage polarization

Diet-induced obesity promotes myelopoiesis in hematopoietic stem cells

Adipose tissue macrophages promote myelopoiesis and monocytosis in obesity

Bone marrow lympho-myeloid malfunction in obesity requires precursor cell-autonomous TLR4

TLR4, TRIF, and MyD88 are essential for myelopoiesis and CD11c(+) adipose tissue macrophage production in obese mice

Palmitate Activates CCL4 Expression in Human Monocytic Cells via TLR4/MyD88 Dependent Activation of NF-kappaB/MAPK/ PI3K Signaling Systems

Effect of lipopolysaccharide on inflammation and insulin action in human muscle

Endotoxins are associated with visceral fat mass in type 1 diabetes

TLR4/MyD88 -mediated CCL2 production by lipopolysaccharide (endotoxin): Implications for metabolic inflammation

Mechanisms of macrophage activation in obesityinduced insulin resistance

Obesity worsens the outcome of influenza virus infection associated with impaired type I interferon induction in mice

Diet-induced obese mice have increased mortality and altered immune responses when infected with influenza virus

Obesity Increases Mortality and Modulates the Lung Metabolome during Pandemic H1N1 Influenza Virus Infection in Mice

Impact of Obesity on Influenza A Virus Pathogenesis, Immune Response, and Evolution

Epigenetic Regulation of TLR4 in Diabetic Macrophages Modulates Immunometabolism and Wound Repair

TNF-alpha regulates diabetic macrophage function through the histone acetyltransferase MOF

Ly6C(Hi) Blood Monocyte/Macrophage Drive Chronic Inflammation and Impair Wound Healing in Diabetes Mellitus

Upregulation of cyclooxygenase-II gene and PGE2 production of peritoneal macrophages in diabetic rats

Epigenetic Regulation of the PGE2 Pathway Modulates Macrophage Phenotype in Normal and Pathologic Wound Repair

CREB pathway links PGE2 signaling with macrophage polarization

Prostaglandin E2 As a Modulator of Viral Infections

The Perfect Storm: Coronavirus (Covid-19) Pandemic Meets Overfat Pandemic. Front Public Health 8

Risk of Infection in Type 1 and Type 2 Diabetes Compared With the General Population: A Matched Cohort Study

Increased airway glucose increases airway bacterial load in hyperglycaemia

Hyperglycemia drives intestinal barrier dysfunction and risk for enteric infection

Glucose depletion in the airway surface liquid is essential for sterility of the airways

Bacterial phagocytosis and intracellular killing by alveolar macrophages in BB rats

Diabetic mice display a delayed adaptive immune response to Mycobacterium tuberculosis

Impaired Recognition of Mycobacterium tuberculosis by Alveolar Macrophages From Diabetic Mice

Increased susceptibility to Mycobacterium tuberculosis infection in a diet-induced murine model of type 2 diabetes

Leptin-deficient mice exhibit impaired host defense in Gramnegative pneumonia

Mice chronically fed high-fat diet have increased mortality and disturbed immune response in sepsis

Defects in early cell recruitment contribute to the increased susceptibility to respiratory Klebsiella pneumoniae infection in diabetic mice

Staphylococcus aureus requires less virulence to establish an infection in diabetic hosts

Obesity Exacerbates Sepsis-Induced Oxidative Damage in Organs

GM-CSF Administration Improves Defects in Innate Immunity and Sepsis Survival in Obese Diabetic Mice

Short-term consumption of a high-fat diet increases host susceptibility to Listeria monocytogenes infection

Metabolic Syndrome and Risk of Cervical Human Papillomavirus Incident and Persistent Infection

Morbid obesity as a risk factor for hospitalization and death due to 2009 pandemic influenza A(H1N1) disease

Obese mice have increased morbidity and mortality compared to non-obese mice during infection with the 2009 pandemic H1N1 influenza virus

The impact of obesity and timely antiviral administration on severe influenza outcomes among hospitalized adults

Association of Initial Viral Load in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Patients with Outcome and Symptoms

Obesity Impairs the Adaptive Immune Response to Influenza Virus

Coronaviridae Study Group of the International Committee on Taxonomy of, V. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2

COVID-19 and comorbidities: A role for dipeptidyl peptidase 4 (DPP4) in disease severity?

Expression of the SARS-CoV-2 cell receptor gene ACE2 in a wide variety of human tissues

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

The Role of Adipocytes and Adipocyte-Like Cells in the Severity of COVID-19 Infections

Histopathology and genetic susceptibility in COVID-19 pneumonia

Is Adipose Tissue a Reservoir for Viral Spread, Immune Activation, and Cytokine Amplification in Coronavirus Disease

The initiation of metabolic inflammation in childhood obesity

Role of the Gut Microbiome in the Pathogenesis of Obesity and Obesity-Related Metabolic Dysfunction

The Intestinal Immune System in Obesity and Insulin Resistance

Depicting SARS-CoV-2 faecal viral activity in association with gut microbiota composition in patients with COVID-19

Gut Microbes and Health: A Focus on the Mechanisms Linking Microbes, Obesity, and Related Disorders

Differences in airway microbiome and metabolome of single lung transplant recipients

Genomewide Association Study of Severe Covid-19 with Respiratory Failure

The COVID-19 Host Genetics Initiative, a global initiative to elucidate the role of host genetic factors in susceptibility and severity of the SARS-CoV-2 virus pandemic

Genome-wide association analyses identify 143 risk variants and putative regulatory mechanisms for type 2 diabetes

The trinity of COVID-19: immunity, inflammation and intervention

Metabolically healthy obesity: facts and fantasies

Contribution of adipogenesis to healthy adipose tissue expansion in obesity

Sex differences in the response to viral infections: TLR8 and TLR9 ligand stimulation induce higher IL10 production in males

Gender differences in cytokine secretion by human peripheral blood mononuclear cells: role of estrogen in modulating LPS-induced cytokine secretion in an ex vivo septic model

Sexual dimorphism in expression of receptors for bacterial lipopolysaccharides in murine macrophages: a possible mechanism for gender-based differences in endotoxic shock susceptibility

Worse progression of COVID-19 in men: Is testosterone a key factor?

Differences in Hematopoietic Stem Cells Contribute to Sexually Dimorphic Inflammatory Responses to High Fat Diet-induced Obesity

Sex Differences in Inflammatory Responses to Adipose Tissue Lipolysis in Diet-Induced Obesity

Sex Differences in the Effect of Inflammation on Subjective Social Status: A Randomized Controlled Trial of Endotoxin in Healthy Young Adults

Sex differences in obesity, lipid metabolism, and inflammation-A role for the sex chromosomes?

Endocrine and metabolic link to coronavirus infection

Obesity and SARS-CoV-2: A population to safeguard

The X-files in immunity: sex-based differences predispose immune responses

Female mice are protected against high-fat diet induced metabolic syndrome and increase the regulatory T cell population in adipose tissue

Prevention of COVID-19 in Older Adults: A Brief Guidance from the International Association for Gerontology and Geriatrics (IAGG) Asia/Oceania region

Association between age and clinical characteristics and outcomes of COVID-19

Is BMI higher in younger patients with COVID-19? Association between BMI and COVID-19 hospitalization by age

Meta-inflammaging at the crossroad of geroscience

Pathophysiology of COVID-19: Why Children Fare Better than Adults?

SARS-CoV-2 infection in children: Transmission dynamics and clinical characteristics

Clinical characteristics and diagnostic challenges of pediatric COVID-19: A systematic review and meta-analysis

Clinical Features, and Disease Severity in Patients With Coronavirus Disease 2019 (COVID-19) in a Children's Hospital in

Glycemic Characteristics and Clinical Outcomes of COVID-19 Patients Hospitalized in the United States

ACE2 Expression in Pancreas May Cause Pancreatic Damage After SARS-CoV-2 Infection

Diabetic ketoacidosis precipitated by Covid-19 in a patient with newly diagnosed diabetes mellitus

Binding of SARS coronavirus to its receptor damages islets and causes acute diabetes

New-Onset Diabetes in Covid-19

Remdesivir for the Treatment of Covid-19 -Preliminary Report

Remdesivir in adults with severe COVID-19: a randomised, doubleblind, placebo-controlled, multicentre trial

A c c e p t e d M a n u s c r i p t 22 A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 25 
