        item: #1 of 47
          id: cord-000354-05lnj3w0
      author: de Vries, Erik
       title: Dissection of the Influenza A Virus Endocytic Routes Reveals Macropinocytosis as an Alternative Entry Pathway
        date: 2011-03-31
       words: 10993
      flesch: 42
     summary: Clathrin mediated endocytosis (CME) has for long been identified and studied as the major route of IAV cell entry [1, 2] and is, by far, the best characterized endocytic pathway. Reversible inhibitors like dynasore [60] offer a major advantage for characterization of IAV entry pathways.
    keywords: actin; cells; dyna; dynamin; dynasore; entry; fcs; fig; iav; iav entry; ind; infection; inhibitors; macropinocytosis; pathway; pbs; presence; serum; virus
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        item: #2 of 47
          id: cord-001017-4qfhltg4
      author: Chatterjee, Dhriti
       title: Microglia Play a Major Role in Direct Viral-Induced Demyelination
        date: 2013-06-20
       words: 6673
      flesch: 37
     summary: For long time, microglia were known to be present in the chronic inflammatory demyelinating plaque to remove myelin from the dead sick neuron in MS patients but the emerging recognition of microglia as CNS resident immune cells and their role in CNS health and diseases stimulated substantial efforts to redefine the role and function of microglia in the regulatory mechanisms of demyelination. RSA59-induced meningitis (Supplementary Figure 1(a) ), and encephalomyelitis (accumulation of inflammatory cell and perivascular cuffing) ( Supplementary Figures 1(b) and 1(c) ) were observed as shown previously [18, 19] (Supplementary Figure 1 ; these data are partly published but for the ready information compiled in one figure. ).
    keywords: cells; cns; demyelination; infected; infection; mhv; mice; microglia; mouse; pbs; rsa59; spinal; virus
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        item: #3 of 47
          id: cord-001065-j4hvyyoi
      author: Boncristiani, Humberto F.
       title: In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
        date: 2013-09-05
       words: 6377
      flesch: 40
     summary: The high concentration of IAPV over other honey bee viruses in these purifications allowed us to strongly dilute the inoculum, decreasing the chances of cross inoculation with another virus. Attempts to isolate pure IAPV directly from naturally infected adult bees were unsuccessful due to co-infection of the bees with other honey bee viruses.
    keywords: bee; bees; colonies; colony; expression; gene; honey; iapv; infection; pbs; pupae; replication; time; treatment; virus; viruses
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        item: #4 of 47
          id: cord-001732-4eyn7pjq
      author: Riede, O
       title: Preclinical safety and tolerability of a repeatedly administered human leishmaniasis DNA vaccine
        date: 2015-04-30
       words: 6343
      flesch: 43
     summary: Considerations for Plasmid DNA Vaccines for Infectious Disease Indications WHO Annex 1: Guidelines for assuring the quality and non-clinical safety evaluation of DNA vaccines Toxicological safety evaluation of DNA plasmid vaccines against HIV-1, Ebola, Severe Acute Respiratory Syndrome, or West Nile virus is similar despite differing plasmid backbones or gene-inserts Biodistribution of DNA plasmid vaccines against HIV-1, Ebola, Severe Acute Respiratory Syndrome, or West Nile virus is similar, without integration, despite differing plasmid backbones or gene inserts Clinical applications of DNA vaccines: current progress CPMP/BWP/3088/99: Note for guidance on the quality, preclinical and clinical aspects of gene transfer medicinal products Marker-free plasmids for biotechnological applicationsimplications and perspectives DNA immunisation with minimalistic expression constructs Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo Minicircle DNA is superior to plasmid DNA in eliciting antigen-specific CD8+ T-cell responses Priming of immune responses to hepatitis B surface antigen with minimal DNA expression constructs modified with a nuclear localization signal peptide Effect of different nuclear localization sequences on the immune responses induced by a MIDGE vector encoding bovine herpesvirus-1 glycoprotein D Intratumoral dispersion, retention, systemic biodistribution, and clearance of a small-size tumor necrosis factor-alpha-expressing MIDGE vector after nonviral in vivo jetinjection gene transfer Combination of MIDGE-Th1 DNA vaccines with the cationic lipid SAINT-18: Studies on formulation, biodistribution and vector clearance ModulaR In addition, CD4 T cells and B cells are activated by DNA vaccines.
    keywords: animals; days; dna; dose; groups; injection; leishdnavax; mice; midge; plasmid; safety; study; th1; vaccine; vector
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        item: #5 of 47
          id: cord-002013-rb9xdzro
      author: Zheng, Xuexing
       title: Treatment with hyperimmune equine immunoglobulin or immunoglobulin fragments completely protects rodents from Ebola virus infection
        date: 2016-04-12
       words: 6042
      flesch: 47
     summary: Outbreaks Chronology: Ebola Virus Disease Clinical management of filovirus-infected patients Organization of patient care during the Ebola hemorrhagic fever epidemic in Kikwit, Democratic Republic of the Congo Emergence of Zaire Ebola virus disease in Guinea Ebola Situation Report -16 Postexposure antibody prophylaxis protects nonhuman primates from filovirus disease Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques Successful treatment of ebola virus-infected cynomolgus macaques with monoclonal antibodies Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp Clinical care of two patients with Ebola virus disease in the United States Two-mAb Cocktail Protects Macaques Against The Makona Variant of Ebola Virus Use of a Reduced (4-Dose) Vaccine Schedule for Postexposure Prophylaxis to Prevent Human Rabies Passive transfer of antibodies protects immunocompetent and imunodeficient mice against lethal Ebola virus infection without complete inhibition of viral replication Postexposure antibody prophylaxis protects nonhuman primates from filovirus disease Animal models for Ebola and Marburg virus infections Backs against the wall: novel and existing strategies used during the 2014-2015 Ebola virus outbreak Treatment of Ebola hemorrhagic fever with blood transfusions from convalescent patients. Plos neglected tropical diseases 6, e1575 Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates Ebola virus can be effectively neutralized by antibody produced in natural human infection Neutralizing antibody fails to impact the course of Ebola virus infection in monkeys The Fc portion of antibody to yellow fever virus NS1 is a determinant of protection against YF encephalitis in mice Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms Natural antibody and complement mediate neutralization of influenza virus in the absence of prior immunity Complement protein C1q reduces the stoichiometric threshold for antibody-mediated neutralization of West Nile virus Ebola virus-like particles produced in insect cells exhibit dendritic cell stimulating activity and induce neutralizing antibodies A quantitative immunochemical measure of the primary interaction between I BSA and antibody Structure of the fusion core and inhibition of fusion by a heptad repeat peptide derived from the S protein of Middle East respiratory syndrome coronavirus X.Z., G.W., Y.Z., H.W., S.H., Y.B., X.Q., S.Y. and X.X. performed the experiments and analyzed the results.
    keywords: antibody; antisera; dpi; ebola; ebov; equine; f(ab′; mice; pbs; treatment; virus; weight
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        item: #6 of 47
          id: cord-002583-cgcf7mgj
      author: Zhuo, Xun-hui
       title: Evaluation of potential anti-toxoplasmosis efficiency of combined traditional herbs in a mouse model
        date: 2017-06-01
       words: 3792
      flesch: 48
     summary: Even though many antigens including rhoptry, surface, and dense granule excreted-secreted antigens were evaluated as potential vaccines (Kur et al., 2009) , there is no licensed vaccine against T. gondii infection clinically making the situation even worse (Jongert et al., 2009; Innes, 2010b) . There exists a possibility that Chinese herbs may provide protection against T. gondii.
    keywords: blood; control; dpi; gondii; group; herbs; mice
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        item: #7 of 47
          id: cord-003915-kje8lvgl
      author: Pigeyre, Laetitia
       title: Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda
        date: 2019-09-17
       words: 9052
      flesch: 43
     summary: Results showed that JcDV infected larvae pre-treated with Calcofluor displayed a significant shorter median time to death (LT50) compared to untreated infected larvae (7 vs. 9 days p.i. for control larvae; p < 0.01) ( Figure 1A and Supplementary Materials Figure S1 ), supporting that the PM can limit JcDV infection. JcDV infection of target cells (eg epidermis, trachea, hemocytes) proceeds by a receptor-dependent mechanism different from intestinal cells
    keywords: binding; calcofluor; capsids; caterpillars; chitin; densovirus; figure; frugiperda; glycans; gut; infection; insect; jcdv; midgut; min; pbs; proteins; results
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        item: #8 of 47
          id: cord-009800-tajkpgkj
      author: Orellana, Mhica V.
       title: An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells
        date: 2003-12-22
       words: 5891
      flesch: 34
     summary: A limited number of oceanographic studies of photosynthetic or growth rates of individual phytoplankton cells or species have been carried out with autoradiography (Iturriaga and Marra 1988) , 14C labeling of individual cells (Rivkin 1990) , and cell division patterns (Weiler and Eppley 1979) . Because it is a soluble protein and a major constituent of phytoplankton cells, Rubisco can be easily detected in individual cells with immunofluorescent probes.
    keywords: activity; affinity; antiserum; binding; cells; cross; light; pbs; photosynthetic; phytoplankton; rates; rubisco; species; subunit
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        item: #9 of 47
          id: cord-011106-h20vbmbo
      author: Takeda, Yohei
       title: Antiviral Activities of Hibiscus sabdariffa L. Tea Extract Against Human Influenza A Virus Rely Largely on Acidic pH but Partially on a Low-pH-Independent Mechanism
        date: 2019-10-16
       words: 5385
      flesch: 48
     summary: Here, acidic PCA and hibiscus acid showed anti-PR8 virus activity. The neutral PCA also showed anti-PR8 virus activity (Fig. 2) .
    keywords: acidic; activity; hib[crude; hibiscus; influenza; mice; pca; pr8; virus
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        item: #10 of 47
          id: cord-013265-qrfi6e5c
      author: Isono, Toshihito
       title: Treatment of severe pneumonia by hinokitiol in a murine antimicrobial-resistant pneumococcal pneumonia model
        date: 2020-10-15
       words: 4594
      flesch: 34
     summary: Taken together, these findings suggested that the antibacterial action of hinokitiol in the lungs of pneumonia mice reduced the production of cytokines or chemokines in BALF and serum. In this study, intratracheal hinokitiol administration reduced neutrophil infiltration and elastase release in the lungs of pneumonia mice, consequently diminishing lung injury and pneumococcal DNA detection in serum.
    keywords: administration; balf; group; hinokitiol; infection; lung; mice; pbs; pneumonia; serum; treatment
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        item: #11 of 47
          id: cord-013526-6fip93l2
      author: Labadie, Thomas
       title: A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion
        date: 2020-10-19
       words: 8109
      flesch: 44
     summary: The presence of viral proteins was then investigated in the lysosomes of BTV infected cells. Altogether, these data established that the majority of BTV virus particles are released in large EVs secreted from mammalian and insect infected cells in vitro.
    keywords: btv; cells; evs; fig; hpi; infected; infection; lysosomes; min; ns3; particles; pbs; protein; unag; viral; virus; virus particles; viruses
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        item: #12 of 47
          id: cord-026866-0hlre9i6
      author: Perruzza, Lisa
       title: Prophylactic Activity of Orally Administered FliD-Reactive Monoclonal SIgA Against Campylobacter Infection
        date: 2020-06-09
       words: 9391
      flesch: 31
     summary: Front Immunol DOI: 10.3389/fimmu.2020.01011 sha: doc_id: 26866 cord_uid: 0hlre9i6 Campylobacter infection is one of the most common causes of bacterial gastroenteritis worldwide and a major global health threat due to the rapid development of antibiotic resistance. Our work emphasizes the potential of FliD as a target for the development of vaccines and supports the concept that orally administered FliD-reactive SIgA can be developed to prevent or mitigate the severity of Campylobacter infections as well as the development of post-infection syndromes.
    keywords: activity; animals; antibodies; bacteria; caa1; campylobacter; ccg4; cells; figure; flid; human; igg; infection; jejuni; mabs; mice; prophylactic; protein; recombinant; siga
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        item: #13 of 47
          id: cord-259094-5qzbctan
      author: Xu, Mei Ling
       title: The effect of dietary intake of the acidic protein fraction of bovine colostrum on influenza A (H1N1) virus infection
        date: 2013-04-26
       words: 2521
      flesch: 46
     summary: Therefore, the effect of total bovine colostrums on influenza virus infection in mice was significantly different from our expectation. Therefore, it is assumed that other components of AFC such as free sialyl oligosaccharides may be beneficial on ameliorating the symptom caused by influenza virus infection.
    keywords: afc; bovine; colostrum; infection; influenza; protein; virus
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        item: #14 of 47
          id: cord-259364-8zoav7b0
      author: Xiao, Yire
       title: Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
        date: 2019-09-09
       words: 4498
      flesch: 51
     summary: Anti- T. rubrum cell wall proteins IgY (anti-trCWP IgY) presented a certain degree of cross-reactivity with different fungi. The strips were then incubated overnight at 4°C with a 1:100 dilution of anti-trCWP IgY (1 mg/mL).
    keywords: anti; cell; igy; pbs; ph7.4; rubrum; specific
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        item: #15 of 47
          id: cord-260834-v254de8k
      author: Lim, Chia Chiu
       title: Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies
        date: 2019-04-15
       words: 8343
      flesch: 47
     summary: The absorbance was measured at OD 405nm within 1 h. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface Designing human antibodies by phage display By-passing immunization: human antibodies from V-gene libraries displayed on phage Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation Molecular considerations for development of phage antibody libraries Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy Structural basis for ebolavirus matrix assembly and budding; protein plasticity allows multiple functions Selecting and screening recombinant antibody libraries Recombinant antibody libraries and selection technologies Isolation of serotype-specific antibodies against dengue virus non-structural protein 1 using phage display and application in a multiplexed serotyping assay Development of recombinant antigen array for simultaneous detection of viral antibodies ClearColi BL21 (DE3)-based expression of Zika virus antigens illustrates a rapid method of antibody production against emerging pathogens Development of prototype filovirus recombinant antigen immunoassays Heterologous expression of Plasmodium vivax apical membrane antigen 1 (PvAMA1) for binding peptide selection Immune TB antibody phage display library as a tool to study B cell immunity in TB infections Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen Advanced technologies for improved expression of recombinant proteins in bacteria: perspectives and applications Recombinant protein expression in Escherichia coli: advances and challenges Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives Adding the desired amount of antibody phage library into the immunotube; 3.
    keywords: antibody; antigen; binding; biopanning; blocking; buffer; crude; fold; library; lysate; m13ko7; phage; ptm; rubi; selection; αubi
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        item: #16 of 47
          id: cord-264267-weat0qs6
      author: Kleine-Weber, Hannah
       title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
        date: 2020-01-21
       words: 7245
      flesch: 41
     summary: As DPP4 needs to be transported to the plasma membrane to be engaged by MERS-CoV S for host cell entry, we next investigated whether the presence of the polymorphic DPP4 residues has an impact on DPP4 cell surface localization. We identified four mutations that reduced MERS-CoV S binding to DPP4 and MERS-CoV S-driven host cell entry without affecting DPP4 expression at the cell surface.
    keywords: analysis; binding; cells; cov; cov s; dpp4; entry; expression; human; mers; pbs; polymorphisms; protein; residues
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        item: #17 of 47
          id: cord-265740-wjdeps3h
      author: Radbel, Jared
       title: Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media
        date: 2020-05-13
       words: 2253
      flesch: 43
     summary: key: cord-265740-wjdeps3h authors: Radbel, Jared; Jagpal, Sugeet; Roy, Jason; Brooks, Andrew; Tischfield, Jay; Sheldon, Michael; Bixby, Christian; Witt, Dana; Gennaro, Maria Laura; Horton, Daniel B.; Barrett, Emily S.; Carson, Jeffrey L.; Panettieri, Reynold A.; Blaser, Martin J. title: Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media date: 2020-05-13 journal: J Mol Diagn DOI: 10.1016/j.jmoldx.2020.04.209 sha: doc_id: 265740 cord_uid: wjdeps3h As the COVID-19 pandemic sweeps across the world, the availability of viral transport media (VTM) has become severely limited, contributing to delays in diagnosis and rationing of diagnostic testing. SARS-CoV-2 detection using standard testing of upper airway secretions requires a nasopharyngeal (NP) or oropharyngeal (OP) swab that is transported to a clinical laboratory using viral transport media (VTM) (https://www.fda.gov/medical-devices/emergency-situations-medical-devices/faqs-diagnostic-testingsars-cov-2#offeringtests, last accessed April 29 2020).
    keywords: cov-2; pbs; samples; sars; transport; vtm
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        item: #18 of 47
          id: cord-267736-rya9w6sh
      author: Kang, Xiaoping
       title: Development of an ELISA-array for simultaneous detection of five encephalitis viruses
        date: 2012-02-27
       words: 2993
      flesch: 42
     summary: The efficacy of the spotting concentration of the capture antibodies was evaluated by virus culture detection, the proper spotting concentration was determined by a combination of minimized cross reaction and higher signal intensity. To make the assay more amenable to multiple virus detection, the assay protocol was optimized.
    keywords: antibodies; antibody; array; assay; detection; elisa; encephalitis; pbs
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        item: #19 of 47
          id: cord-273035-sewfb3q8
      author: Kang, Xixiong
       title: Proteomic Fingerprints for Potential Application to Early Diagnosis of Severe Acute Respiratory Syndrome
        date: 2005-01-01
       words: 4137
      flesch: 44
     summary: Among the retrospective samples, only 74 were selected from probable patients whose blood samples were collected with onset of fever within 7 days at the time of admission (acute SARS patients; Table 1 ). Serum samples were grouped into acute SARS (n = 74; <7 days after onset of fever) and non-SARS [n = 1067; fever and influenza A (n = 203), pneumonia (n = 176); lung cancer (n = 29); and healthy controls (n = 659)] cohorts.
    keywords: mass; non; patients; peak; samples; sars; serum; set; spectra
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        item: #20 of 47
          id: cord-274396-l611eisi
      author: Park, Su-Jin
       title: Antiviral Efficacies of FDA-Approved Drugs against SARS-CoV-2 Infection in Ferrets
        date: 2020-05-22
       words: 4377
      flesch: 37
     summary: Comparisons of virus titers and shedding periods in antiviral-drug-treated ferrets. To further evaluate virus titers in tissues, three ferrets from each group were euthanized at 4 and 8 dpi, and virus titers were measured in nasal turbinate and lungs.
    keywords: control; cov-2; dpi; ferrets; group; infection; pbs; sars; titers; virus
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        item: #21 of 47
          id: cord-275635-d50bxe7c
      author: Yuan, Xiaomin
       title: Efficacy and immunogenicity of recombinant swinepox virus expressing the A epitope of the TGEV S protein
        date: 2015-07-31
       words: 3999
      flesch: 49
     summary: Domains in the peplomer glycoprotein Bacterial expression of antigenic sites A and D in the spike protein of transmissible gastroenteritis virus and evaluation of their inhibitory effects on viral infection Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing spike genes from porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus Construction, safety and immunogenicity analysis of attenuated Salmonella typhimurium harbouring TGEV DNA vaccine Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus Expression of swine transmissible gastroenteritis virus envelope antigens on the surface of infected cells: epitopes externally exposed Clinical evaluation of transmissible gastroenteritis virus vaccines and vaccination procedures for inducing lactogenic immunity in sows A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene 25 years and counting: Centers for Disease Control and Prevention identifies opportunities and challenges for diabetes prevention and control Development of a recombinant vaccinia-rabies vaccine for oral vaccination of foxes against rabies Recombinant fowlpox virus inducing protective immunity in non-avian species Recombinant swinepox virus expressing betagalactosidase: investigation of viral host range and gene expression levels in cell culture Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein Replication and expression of a swinepox virus vector delivering feline leukemia virus Gag and Env to cell lines of swine and feline origin Feline B7.1 and B7.2 proteins produced from swinepox virus vectors are natively processed and biologically active: potential for use as nonchemical adjuvants First insights into the protective effects of a recombinant swinepox virus expressing truncated MRP of Streptococcus suis type 2 in mice Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs A novel vaccine against Streptococcus equi ssp. key: cord-275635-d50bxe7c authors: Yuan, Xiaomin; Lin, Huixing; Fan, Hongjie title: Efficacy and immunogenicity of recombinant swinepox virus expressing the A epitope of the TGEV S protein date: 2015-07-31 journal: Vaccine DOI: 10.1016/j.vaccine.2015.06.057 sha: doc_id: 275635 cord_uid: d50bxe7c
    keywords: cells; mice; protein; rspv; tgev; virus; wtspv
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        item: #22 of 47
          id: cord-275779-ocbygkyb
      author: Ye, Zi-Wei
       title: Antibody-Dependent Cell-Mediated Cytotoxicity Epitopes on the Hemagglutinin Head Region of Pandemic H1N1 Influenza Virus Play Detrimental Roles in H1N1-Infected Mice
        date: 2017-03-21
       words: 5468
      flesch: 35
     summary: inTrODUcTiOn Influenza viruses, as one of the major zoonotic pathogens, have continuously caused global health concerns due to their high propensity for unpredictable genetic mutation in major surface antigens, hemagglutinin (HA), and neuramindase. Neutralizing antibodies have traditionally been thought to provide protection against influenza viruses.
    keywords: adcc; antibodies; antibody; cells; figure; influenza; mice; mouse; vaccinated; virus
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        item: #23 of 47
          id: cord-276732-u2d1z4ip
      author: Mauri, Tommaso
       title: Intraperitoneal adoptive transfer of mesenchymal stem cells enhances recovery from acid aspiration acute lung injury in mice
        date: 2017-03-06
       words: 4450
      flesch: 42
     summary: The acute respiratory distress syndrome Soluble receptor for advanced glycation end-products predicts impaired alveolar fluid clearance in acute respiratory distress syndrome Subphenotypes in acute respiratory distress syndrome: latent class analysis of data from two randomised controlled trials Treating ARDS: new hope for a tough problem Angiotensin-(1-7) improves oxygenation, while reducing cellular infiltrate and fibrosis in experimental acute respiratory distress syndrome Human mesenchymal stem cell microvesicles for treatment of Escherichia coli endotoxin-induced acute lung injury in mice Stem cell therapy for acute respiratory distress syndrome: a promising future Allogenic human mesenchymal stem cells for treatment of E. coli endotoxin-induced acute lung injury in the ex vivo perfused human lung Effects of intratracheal mesenchymal stromal cell therapy during recovery and resolution after ventilator-induced lung injury Human mesenchymal stem cells reduce the severity of acute lung injury in a sheep model of bacterial pneumonia Treatment of acute respiratory distress syndrome with allogeneic adipose-derived mesenchymal stem cells: a randomized, placebocontrolled pilot study Lung injury and recovery in a murine model of unilateral acid aspiration: functional, biochemical, and morphologic characterization Mechanical ventilationassociated lung fibrosis in acute respiratory distress syndrome: a significant contributor to poor outcome Alveolar pentraxin 3 as an early marker of microbiologically confirmed pneumonia: a threshold-finding prospective observational study The yin-yang of long pentraxin PTX3 in inflammation and immunity Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation Proteomic analysis of tumor necrosis factor-alphainduced secretome of human adipose tissue-derived mesenchymal stem cells Mesenchymal stromal cell-derived PTX3 promotes wound healing via fibrin remodeling Mesenchymal stem cells: mechanisms of potential therapeutic benefit in ARDS and sepsis An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode Phenotypic overlap between MMP-13 and the plasminogen activation system during wound healing in mice Inhibition of pulmonary fibrosis by the chemokine IP-10/CXCL10 Intraperitoneally infused human mesenchymal stem cells form aggregates with mouse immune cells and attach to peritoneal organs Aspiration-induced lung injury Epidemiology, patterns of care, and mortality for patients with acute respiratory distress syndrome in intensive care units in 50 countries Therapeutic effects of human mesenchymal stem cell-derived microvesicles in severe pneumonia in mice Unilateral acid aspiration augments the effects of ventilator lung injury in the contralateral lung Spontaneous effort during mechanical ventilation: maximal injury with less positive endexpiratory pressure Elevated plasma and alveolar levels of soluble receptor for advanced glycation endproducts are associated with severity of lung dysfunction in ARDS patients Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice Long pentraxin PTX3 deficiency worsens LPS-induced acute lung injury Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6 Regulation of leukocyte recruitment by the long pentraxin PTX3 Mesenchymal stem (stromal) cells for treatment of ARDS: a phase 1 clinical trial genetic variations affect the risk of Pseudomonas aeruginosa airway colonization in cystic fibrosis patients Genetic PTX3 deficiency and aspergillosis in stem-cell transplantation Implementation of a pan-genomic approach to investigate holobiont-infecting microbe interaction: a case report of a leukemic patient with invasive mucormycosis We are thankful to all the personnel working in the University of Milan-Bicocca, San Gerardo Hospital and Humanitas Institute for the continuous support and collaboration. Histology found reduction of lung injury, albeit non-significant (Table 1 ).
    keywords: acid; acute; effects; injury; lung; mice; mscs; ptx3; −/−
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        item: #24 of 47
          id: cord-277054-eq4obbte
      author: Kaur, Manpreet
       title: Rabies DNA vaccine: No impact of MHC Class I and Class II targeting sequences on immune response and protection against lethal challenge
        date: 2009-03-26
       words: 6916
      flesch: 40
     summary: key: cord-277054-eq4obbte authors: Kaur, Manpreet; Rai, Anant; Bhatnagar, Rakesh title: Rabies DNA vaccine: No impact of MHC Class I and Class II targeting sequences on immune response and protection against lethal challenge date: 2009-03-26 journal: Vaccine DOI: 10.1016/j.vaccine.2009.01.128 sha: doc_id: 277054 cord_uid: eq4obbte Rabies is progressive fatal encephalitis. Rabies DNA vaccine has also been found to be highly efficient in large size mammals [21] .
    keywords: antibodies; antibody; antigen; cells; class; constructs; dna; glycoprotein; mhc; mice; pbs; rabies; response; vaccination; vaccine; virus
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        item: #25 of 47
          id: cord-278802-bverdk5w
      author: Zhou, Yefei
       title: Immune response of AA broilers to IBV H120 vaccine and sodium new houttuyfonate
        date: 2010-12-31
       words: 3170
      flesch: 51
     summary: after primary vaccination, the values of groups 1-4 were larger than those of IL-2 and PBS groups. At 21, 28 and 35 dpi, the values of 200 mg/L group were significantly larger than those of IL-2 or PBS groups (p < 0.05).
    keywords: dpi; groups; pbs; serum; snh
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        item: #26 of 47
          id: cord-292862-ezrkg0dc
      author: Myerson, Jacob W.
       title: Supramolecular Organization Predicts Protein Nanoparticle Delivery to Neutrophils for Acute Lung Inflammation Diagnosis and Treatment
        date: 2020-04-18
       words: 14319
      flesch: 42
     summary: Our data thus indicate that NAP tropism for neutrophils in inflamed mouse lungs may be recapitulated in human lungs. 6, 10, 14, 18 Single cell suspensions prepared from mouse lungs were probed by flow cytometry to further characterize pulmonary neutrophils in naïve mice and in mice following LPS-induced inflammation.
    keywords: albumin; anti; cells; data; dbco; figure; human; igg; inflammation; injury; liposomes; lps; lungs; lysozyme; mice; minutes; nanoparticles; naps; naïve; neutrophils; nps; protein; pulmonary; uptake
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        item: #27 of 47
          id: cord-295033-5fd9bu60
      author: Parma, Y.R.
       title: Antibodies anti-Shiga toxin 2 B subunit from chicken egg yolk: Isolation, purification and neutralization efficacy
        date: 2011-09-15
       words: 6063
      flesch: 42
     summary: The data suggest that immunization of hens with Stx2B could be a strategy to obtain at low cost a relatively high concentration of anti-Stx2 egg yolk IgY, able to neutralize Stx2 lethal activity. The data suggest that immunization of hens with Stx2B could be a strategy to obtain at low cost a relatively high concentration of anti-Stx2 egg yolk IgY, able to neutralize Stx2 lethal activity.
    keywords: antibodies; antibody; chicken; coli; egg; igg; igy; shiga; stx2; toxin; yolk
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        item: #28 of 47
          id: cord-295416-y3lvcjqd
      author: Eichinger, Katherine M.
       title: Prefusion RSV F Immunization Elicits Th2-Mediated Lung Pathology in Mice When Formulated With a Th2 (but Not a Th1/Th2-Balanced) Adjuvant Despite Complete Viral Protection
        date: 2020-07-29
       words: 9471
      flesch: 39
     summary: Taken together, these data indicate that RSV PreF immunization formulated with Th1-skewing adjuvants, like Advax-SM, provide protection against RSV infection in naïve BALB/c mice as compared to alum by inhibiting viral replication without eliciting enhanced pulmonary pathology. Earlier work found that higher titers of neutralizing antibody were associated with RSV PreF immunization formulated with Th1/Th2-balanced adjuvants (39) .
    keywords: advax; alum; antibody; cells; dpi; figure; immunization; mice; neutralizing; pref; rsv; th1; th2; virus
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        item: #29 of 47
          id: cord-298224-flyx85lr
      author: Hibbitts, Alan J.
       title: In Vitro and In Vivo Assessment of PEGylated PEI for Anti-IL-8/CxCL-1 siRNA Delivery to the Lungs
        date: 2020-06-27
       words: 11359
      flesch: 48
     summary: Furthermore, PEI anti-CXCL-1 siRNA nanoparticles were also found to demonstrate reduced gene expression compared to their non-targeting controls although this was not found to be significant due to the variability in the NT-siRNA-treated animals. Twin-stage impinger cell studies highlighted the significantly enhanced ability of PEI-PEG siRNA nanoparticles to be nebulised, traverse the mucus lining of airway epithelial cells and reduce gene and protein expression compared to PEI siRNA nanoparticles.
    keywords: animals; anti; cells; cxcl-1; delivery; expression; figure; gene; il-8; levels; lpeg sirna; lps; nebulisation; pbs; pei; samples; sirna; sirna nanoparticles; size
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        item: #30 of 47
          id: cord-303381-xvzhb7ix
      author: TSURUTA, Yuya
       title: The requirement of environmental acidification for Ibaraki virus infection to host cells
        date: 2015-08-28
       words: 1989
      flesch: 45
     summary: In other words, the expression level of those proteases affects the susceptibility of the host cells to virus infection. Figure 3b shows the viability of HmLu-1 after a series of treatment without virus infection, and no significant difference was observed (P=0.983, Welch's t-test).
    keywords: cells; endosomal; ibav; infection; virus
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        item: #31 of 47
          id: cord-305648-majanu8l
      author: Schountz, Tony
       title: Rapid Field Immunoassay for Detecting Antibody to Sin Nombre Virus in Deer Mice
        date: 2007-10-17
       words: 1639
      flesch: 46
     summary: Blood samples from 222 deer mice were collected during 3 trapping sessions in the summer of 2006, and 39 samples were scored as positive in the fi eld by PAGEIA; 183 were negative by the fi eld PAGEIA, repeat laboratory PAGEIA, and the standard EIA in the laboratory. Emerg Infect Dis DOI: 10.3201/eid1310.070383 sha: doc_id: 305648 cord_uid: majanu8l We developed a 1-hour field enzyme immunoassay (EIA) for detecting antibody to Sin Nombre virus in deer mice (Peromyscus maniculatus).
    keywords: eld; laboratory; pageia; protein
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        item: #32 of 47
          id: cord-308461-4lhh3du0
      author: Ueki, Hiroshi
       title: Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS
        date: 2020-01-29
       words: 8359
      flesch: 35
     summary: The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors T cell response in the lung following influenza virus infection Neutrophil trails guide influenza-specific CD8 + T cells in the airways Live imaging of influenza infection of the trachea reveals dynamic regulation of CD8 + T cell motility by antigen Imaging cell interaction in tracheal mucosa during influenza virus infection using two-photon intravital microscopy Three phases of CD8 T cell response in the lung following H1N1 influenza infection and sphingosine 1 phosphate agonist therapy Multi-spectral fluorescent reporter influenza viruses (Color-flu) as powerful tools for in vivo studies Molecular determinants of virulence and stability of a reporter-expressing H5N1 influenza A virus Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein Live imaging of the lung A transgenic mouse line that retains Cre recombinase activity in mature oocytes irrespective of the cre ransgene transmission Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre-and Flp-mediated recombination TrackMate: an open and extensible platform for single-particle tracking The role of neutrophils during mild and severe influenza virus infections of mice Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis The effects of acute neutrophil depletion on resolution of acute influenza infection, establishment of tissue resident memory (TRM), and heterosubtypic immunity Antibodies against neutrophil LY6G do not inhibit leukocyte recruitment in mice in vivo Ly6G ligation blocks recruitment of neutrophils via a β2-integrin-dependent mechanism Spectral imaging: principles and applications Multispectral imaging in biology and medicine: slices of life A robust and high-throughput Cre reporting and characterization system for the whole mouse brain Regulating the adaptive immune response to respiratory virus infection A systems analysis identifies a feedforward inflammatory circuit leading to lethal influenza infection Innate immunity to influenza virus infection Intravascular staining for discrimination of vascular and tissue leukocytes In vivo compartmental analysis of leukocytes in mouse lungs A study of the pulmonary circulation by the transillumination method Pulmonary capillary recruitment during airway hypoxia in the dog The behavior of the arterioles and capillaries of the lung Capillary recruitment during airway hypoxia: role of pulmonary artery pressure Capillaroscopic appearance of the pulmonary alveoli in the living dog Diffusing capacity of the lung during hypoxia: role of capillary recruitment Intrapulmonary blood flow redistribution during hypoxia increases gas exchange surface area The normal behavior of the pulmonary blood vessels with observations on the intermittence of the flow of blood in the arterioles and capillaries An experimental model for simultaneous quantitative analysis of pulmonary micro-and macrocirculation during unilateral hypoxia in vivo A thoracic window for observation of the lung in a living animal Microscopic observation of the lung in vivo Precapillary oxygenation contributes relevantly to gas exchange in the intact lung Pulmonary microcirculatory observations in vivo under physiological conditions Direct measurement of pulmonary capillary transit times Intravital laser confocal microscopy of pulmonary edema resulting from intestinal ischemia-reperfusion injury in the rat Physiological neutrophil sequestration in the lung: visual evidence for localization in capillaries Measurement of lung microvascular pressure in the intact anesthetized rabbit by the micropuncture technique Emergency granulopoiesis promotes neutrophil-dendritic cell encounters that prevent mouse lung allograft acceptance Capillary perfusion patterns in single alveolar walls Measurement of microhemodynamics in the ventilated rabbit lung by intravital fluorescence microscopy Donor pulmonary intravascular nonclassical monocytes recruit recipient neutrophils and mediate primary lung allograft dysfunction Leukocyte kinetics in pulmonary microcirculation: intravital fluorescence microscopic study The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis Temporal capillary perfusion patterns in single alveolar walls of intact dogs Aspirin-triggered 15-epi-lipoxin A4 regulates neutrophil-platelet aggregation and attenuates acute lung injury in mice Sites of leukocyte sequestration in the pulmonary microcirculation CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses Contribution of selectins to leucocyte sequestration in pulmonary microvessels by intravital microscopy in rabbits Lung vaso-occlusion in sickle cell disease mediated by arteriolar neutrophil-platelet microemboli Leukocyte margination in alveolar capillaries: interrelationship with functional capillary geometry and microhemodynamics Neutrophils disturb pulmonary microcirculation in sepsis-induced acute lung injury
    keywords: cells; concentration; excitation; fig; fluorescent; imaging; influenza; laser; lung; mice; mouse; photon; pulmonary; solution; suction; system; virus; vivo; window
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        item: #33 of 47
          id: cord-309742-fd1qmr87
      author: Slepushkin, Vladimir A.
       title: Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains
        date: 2016-12-14
       words: 5542
      flesch: 42
     summary: Cleavage of hemagglutinin is an important, and often a host-limiting, step of influenza virus infection. In contrast, influenza virus infection resulted from binding to the apical plasma membrane ((32) and this study), similar to coronavirus (42, 43) and measles virus (44) .
    keywords: airway; apical; cells; epithelia; human; infection; influenza; japan; strain; surface; virus; x31
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        item: #34 of 47
          id: cord-327568-5vo4nmei
      author: Tosini, Fabio
       title: Delivery of SA35 and SA40 peptides in mice enhances humoral and cellular immune responses and confers protection against Cryptosporidium parvum infection
        date: 2019-05-15
       words: 7412
      flesch: 46
     summary: Values from control mice are between 33 and 29 cycles, equivalent to DNA amounts from 6 to 100 oocysts, whereas values from immunised mice are between 39 to 35 cycles, equivalent to DNA amounts from 0.1 to 5 oocysts been the first example of effective prevention for neonates [48] and it has been extensively applied as part of the WHO Maternal and Neonatal Tetanus Elimination (MNTE) program. Neonates born from immunised mice and infected with 1 × 10(5) oocysts showed a significant reduction of oocysts and intestinal forms (23 and 42%, respectively).
    keywords: cells; control; cryptosporidium; immunisation; mice; mix; oocysts; parvum; pbs; sa35; sa35/40; sa40
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        item: #35 of 47
          id: cord-332221-6ea6gz9s
      author: Li, Guiping
       title: Insulin-Like Growth Factor 1 Regulates Acute Inflammatory Lung Injury Mediated by Influenza Virus Infection
        date: 2019-11-26
       words: 6808
      flesch: 42
     summary: The level of IGF1 protein expression was also upregulated at 12, 24, 48, and 72 h post-infection of PR8 ( Figure 1C ) and following the increasing of MOI at 48 h post-infection in A549 cells ( Figure 1D) . Figures 1E,F show that the level of IGF1 protein expression in the A549 cells following PR8 infection was consistent with the level of IGF1 mRNA; however, such upregulation times were lower than the level of mRNA.
    keywords: expression; group; igf1; infection; influenza; lung; mice; pbs; pr8; pr8 infection; protein; virus
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        item: #36 of 47
          id: cord-333639-usgpe1cz
      author: Zuwala, Kaja
       title: Macromolecular prodrugs of ribavirin: Polymer backbone defines blood safety, drug release, and efficacy of anti-inflammatory effects
        date: 2018-04-10
       words: 9368
      flesch: 43
     summary: In this work, to accommodate the synthetic diversity of polymers, we used five different RAFT agents (Table 1) and we see no correlation of polymer activity (Table 2 ) and the RAFT agent used for the polymer synthesis. Herein, we aimed to investigate albumin binding for the library of anionic polymers, with and without conjugation of ribavirin.
    keywords: activity; agents; albumin; antiviral; binding; cells; drug; equiv; fig; macromolecular; mmol; polyanions; polymers; prodrugs; rbv; reaction; release; ribavirin; spectrum
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        item: #37 of 47
          id: cord-334165-7gfk554m
      author: Stadlbauer, Daniel
       title: SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup
        date: 2020-04-17
       words: 5305
      flesch: 66
     summary: Coating ELISA plates (day 1). After the blocking incubation in step 3a, substep iii, remove ELISA plates from the RT incubator and throw off the blocking solution.
    keywords: elisa; pbs; plate; protein; spike; wells
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        item: #38 of 47
          id: cord-335121-ro3x3qa3
      author: Ingram, George A.
       title: A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis
        date: 1988-04-30
       words: 3124
      flesch: 46
     summary: key: cord-335121-ro3x3qa3 authors: Ingram, George A.; Al-Yaman, Fadwa title: A comparative assessment of four serological methods used in the detection and measurement of anti-parasite antibodies in the serum of the amphibian, bufo viridis date: 1988-04-30 journal: International Journal for Parasitology DOI: 10.1016/0020-7519(88)90147-6 sha: doc_id: 335121 cord_uid: ro3x3qa3 Abstract Antibodies against Crithidia fasciculata choanomastigotes were detected in green toad (Bufo viridis) sera by direct agglutination, indirect haemagglutination (IHA), complement-fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA), Correlation coefficients (r) were calculated for comparisons between each of the techniques and regression formulae derived in order to convert antibody levels as determined by one immunological method to that of another. This implies that the above two techniques have similar sensitivities in the screening of normal BVS for anti-parasite antibodies.
    keywords: antibodies; antibody; antigen; control; elisa; parasite; sera; titres
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        item: #39 of 47
          id: cord-335310-61wibso4
      author: Chen, Hui-Wen
       title: Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection
        date: 2016-08-15
       words: 5491
      flesch: 35
     summary: The result demonstrates the feasibility of studying the evolution of nanoparticle protein corona formation using nanoparticle tracking analysis, which reveals changes in light scattering and size simultaneously. structural characterization of the spike protein From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis A truncated receptorbinding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody A simple method of estimating fifty per cent endpoints A Laboratory Manual for the Isolation and Identification of Avian Pathogens Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan Quantitative profiling of the protein coronas that form around nanoparticles Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))
    keywords: analysis; antigen; corona; coronavirus; fig; free; ibv; nanoparticles; pbs; protein; spike; svlps; vaccine; virus
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        item: #40 of 47
          id: cord-335591-r0x8yaqj
      author: Ohnishi, Kazuo
       title: Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
        date: 2007-11-28
       words: 3145
      flesch: 61
     summary: The causative agent was identified as SARS coronavirus (SARS- CoV) (1,2) . This incubation is done by placing the PVDF membrane in the hybridization bag with 5 ml of antibody solution.
    keywords: antigen; cells; cov; elisa; pbs; sars; solution
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        item: #41 of 47
          id: cord-338108-3rn6fwx3
      author: Jin, Yi
       title: Immunomodulatory Activity and Protective Effects of Polysaccharide from Eupatorium adenophorum Leaf Extract on Highly Pathogenic H5N1 Influenza Infection
        date: 2013-09-18
       words: 3974
      flesch: 45
     summary: In conclusion, our study demonstrates that EAP leaf extract is a prophylactic and immune enhancement agent against H5N1 influenza virus infection. Mice were anesthetized with Zotile (Virbac, France) intramuscularly at 15 mg/kg (body weight) and then infected intranasally with 120 plaqueforming units (PFU) of H5N1 influenza virus in 50 L.
    keywords: cells; eap; effect; h5n1; il-6; infection; influenza; mice; pbs; treatment; virus
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        item: #42 of 47
          id: cord-339694-sp212tai
      author: Jiang, Xinpeng
       title: A phase trial of the oral Lactobacillus casei vaccine polarizes Th2 cell immunity against transmissible gastroenteritis coronavirus infection
        date: 2016-03-28
       words: 6830
      flesch: 37
     summary: This vaccine could induce TGEV antibody immune responses in both the humoral and mucosal immune systems. The pig model was developed to study intestinal mucosal immune responses (Ruan and Zhang 2013) .
    keywords: cells; et al; expression; group; immune; immunization; infection; lactobacillus; mucosal; pbs; piglets; response; tgev; vaccine
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        item: #43 of 47
          id: cord-340125-il35gs97
      author: Jayapal, Manikandan
       title: Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcεRI-aggregation reveals a complex network of genes involved in inflammatory responses
        date: 2006-08-16
       words: 7398
      flesch: 42
     summary: Mast cells in innate immunity Mast cells to the defense Mast cells in autoimmune disease Mast cells The diverse potential effector and immunoregulatory roles of mast cells in allergic disease Transcriptional response of human mast cells stimulated via the FcεRI and identification of mast cells as a source of IL-11 The receptor with high affinity for IgE Signalling through the high-affinity IgE receptor FcεRI Roles of mast cells and basophils in innate and acquired immunity Complexity and redundancy in the pathogenesis of asthma: reassessing the roles of mast cells and T cells Mast cells can amplify airway reactivity and features of chronic inflammation in an asthma model in mice An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model Mast cells phagocytosis of FimH expressing enterobacteria Histamine-induced activation of human lung macrophages key: cord-340125-il35gs97 authors: Jayapal, Manikandan; Tay, Hwee Kee; Reghunathan, Renji; Zhi, Liang; Chow, Kah Kiong; Rauff, Mary; Melendez, Alirio J title: Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcεRI-aggregation reveals a complex network of genes involved in inflammatory responses date: 2006-08-16 journal: BMC Genomics DOI: 10.1186/1471-2164-7-210 sha: doc_id: 340125 cord_uid: il35gs97 BACKGROUND:
    keywords: activation; aggregation; anti; cells; cytokines; expression; fcεri; genes; ige; immune; mast; mast cells; responses; role; sensitization; table
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        item: #44 of 47
          id: cord-343409-oao75pzy
      author: Hayward, Joshua A
       title: Identification of diverse full-length endogenous betaretroviruses in megabats and microbats
        date: 2013-03-27
       words: 11607
      flesch: 45
     summary: All of the reference sequences were downloaded from NCBI (Additional file 2: Table S5 ) and aligned with bat sequences using MUSCLE [68] . The gag, pol, and env genes of each genome sequence were translated into protein sequences using the CLC Main Workbench 6.6 (CLC Bio).
    keywords: analysis; bat; bats; betaretroviruses; env; export; gag; gene; group; like; ltr; orf; pbs; pol; protein; pverv; sequence; trna; type; βervs
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        item: #45 of 47
          id: cord-347661-q9lgliph
      author: Zevenhoven-Dobbe, Jessika C.
       title: Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins
        date: 2007-11-28
       words: 6884
      flesch: 45
     summary: key: cord-347661-q9lgliph authors: Zevenhoven-Dobbe, Jessika C.; Wassenaar, Alfred L. M.; van der Meer, Yvonne; Snijder, Eric J. title: Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins date: 2007-11-28 journal: SARS- and Other Coronaviruses DOI: 10.1007/978-1-59745-181-9_16 sha: doc_id: 347661 cord_uid: q9lgliph The importance of monospecific antisera for the experimental analysis of viral proteins is undisputed. Usually, metabolically labeled protein lysates (e.g., 35 S-methionine-labeled) are used for immunoprecipitation studies and samples are run on SDS-PAGE gels that can then be used for autoradiography.
    keywords: antigen; antisera; antiserum; cells; immunization; min; pbs; peptides; protein; rabbit; sds; target; use
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        item: #46 of 47
          id: cord-351498-bmq6zcb0
      author: Martínez-Sernández, Victoria
       title: Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis
        date: 2018-03-21
       words: 8322
      flesch: 34
     summary: Evaluation of a diagnostic monoclonal antibody-based capture enzyme-linked immunosorbent assay for detection of a 26-to 28-kd Fasciola hepatica coproantigen in cattle Diagnosing human anisakiasis: recombinant Ani s 1 and Ani s 7 allergens versus the UniCAP 100 fluorescence enzyme immunoassay The sensitivity and specificity of two methods for detecting Fasciola infections in cattle The life cycle of Fasciola hepatica Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity Determining the prevalence and seasonality of Fasciola hepatica in pasture-based dairy herds in Ireland using a bulk tank milk ELISA Characterisation of a novel Kunitztype molecule from the trematode Fasciola hepatica Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase Associations between anti-Fasciola hepatica antibody levels in bulk-tank milk samples and production parameters in dairy herds Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle Measurement of antibodies to gastrointestinal nematodes and liver fluke in meat juice of beef cattle and associations with carcass parameters Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle Use of a pre-selected epitope of cathepsin-L1 in a highly specific peptide-based immunoassay for the diagnosis of Fasciola hepatica infections in cattle Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease Bridging gaps in the molecular phylogeny of the Lymnaeidae (Gastropoda: Pulmonata), vectors of Fascioliasis Ani s 1 and Ani s 7 recombinant allergens are able to differentiate distinct Anisakis simplex-associated allergic clinical disorders The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach Across intramammalian stages of the liver fluke Fasciola hepatica: a proteomic study Isolation of a cDNA encoding Fasciola hepatica cathepsin L2 and functional expression in Saccharomyces cerevisiae Fasciola hepatica -monitoring the milky way? The use of tank milk for liver fluke monitoring in dairy herds as base for treatment strategies International handbook of foodborne pathogens Sandwich enzyme-linked immunosorbent assay for detection of excretory secretory antigens in humans with fascioliasis Evaluation of Fas2-ELISA for the serological detection of Fasciola hepatica infection in humans Application of a coproantigen ELISA as an indicator of efficacy against multiple life stages Fasciola hepatica infections in sheep The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human fasciolosis Successful DNA immunisation of rats against fasciolosis Evaluation of a recombinant cathepsin L1 ELISA and comparison with the Pourquier and ES ELISA for the detection of antibodies against Fasciola hepatica Advances in refolding of proteins produced in E. coli An extended study of seroprevalence of anti-Anisakis simplex IgE antibodies in Norwegian blood donors Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein Rapid enhanced MM3-COPRO ELISA for detection of Fasciola coproantigens Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control Direct and indirect affection of the central nervous system by Fasciola infection Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario Optimized serodiagnosis of sheep fascioliasis by Fast-D protein liquid chromatography fractionation of Fasciola hepatica excretory-secretory antigens An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3)
    keywords: antibodies; antigens; cattle; elisa; et al; fasciola; hepatica; mm3; recombinant; rfhpcl1; samples; sera; sheep
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        item: #47 of 47
          id: cord-356207-tpn5cg4n
      author: Beniac, Daniel R
       title: Architecture of the SARS coronavirus prefusion spike
        date: 2006-07-16
       words: 805
      flesch: 32
     summary: Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation Functional integrity of intravenous immunoglobulin following irradiation with a virucidal dose of gamma radiation Controlled gamma-irradiation mediated pathogen inactivation of human urokinase preparations with significant recovery of enzymatic activity Detection of airborne severe acute respiratory syndrome (SARS) coronavirus and environmental contamination in SARS outbreak units EMAN: semiautomated software for highresolution single-particle reconstructions SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields EMAN: semiautomated software for highresolution single-particle reconstructions The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles Multi-resolution contour-based fitting of macromolecular structures Structure of SARS coronavirus spike receptor-binding domain complexed with receptor Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state UCSF Chimera--a visualization system for exploratory research and analysis EMAN: semiautomated software for highresolution single-particle reconstructions Structure of the hemagglutinin precursor cleavage site, a determinant of influenza pathogenicity and the origin of the labile conformation Situs: A package for docking crystal structures into low-resolution maps from electron microscopy After washing in PBS-G (PBS pH7.2, 0.2% glycine), grids were then incubated in primary antibody diluted in PBS-G-BSA or convalescent patient serum diluted in PBS, followed by washes in PBS-G and then incubated in secondary antibody (conjugated to 5 or 10 nm gold), followed again by washing in PBS-G. Grids were fixed (1% paraformaldehyde, 1% glutaraldehyde in PBS), washed in deionised water and negatively stained in either 2% uranyl acetate or 2% methylamine tungstate (Nanoprobes, Yaphank, NY.).
    keywords: electron; grids; pbs; sars
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