# bib

id: cord-269013-ge7nmgsa
author: Vui, Dam Thi
title: Complete Genome Sequence of Porcine Epidemic Diarrhea Virus in Vietnam
date: 2014-08-14
extension: .txt
mime: text/plain
words: 761
sentence: 24
flesch: 40
cache: cache/cord-269013-ge7nmgsa.txt
txt: txt/cord-269013-ge7nmgsa.txt
summary:
  Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence A new coronavirus-like particle associated with diarrhea in swine Mutations in the spike gene of porcine epidemic diarrhea with growth adaptation in vitro and attenuation of virulence in vivo Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China Genetic diversity of ORF3 and spike genes of porcine epidemic diarrhea virus in Thailand Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Propagation of the virus of porcine epidemic diarrhea in cell culture Rapid amplification of 5' cDNA ends Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines New variants of porcine epidemic diarrhea virus, China We are grateful to National Research Council of Thailand for funding this research.


id: cord-001956-jpk854i3
author: Choe, Se-Eun
date: 2016-02-18
extension: .txt
mime: text/plain
words: 661
sentence: 22
flesch: 41
cache: cache/cord-001956-jpk854i3.txt
txt: txt/cord-001956-jpk854i3.txt
title:
  Complete Genome Sequence of a Porcine Epidemic Diarrhea Virus Strain from Vietnam, HUA-14PED96, with a Large Genomic Deletion

summary:
  The complete genome sequence of PEDV strain HUA-14PED96 has been deposited in GenBank under accession no. KT941120. The length of the HUA-14PED96 genome is 27,966 nucleotides (nt), excluding the 3= poly (A) tail, and is arranged as follows: The complete genome sequence of HUA-14PED96 shows high nucleotide sequence identity (98.6%) with the highly virulent U.S. PEDV strain (USA/IOWA/18984/2013) and relatively lower identity (97.5%) with a Vietnamese strain (VN/KCHY-310113/2013).


id: cord-001658-algzczs8
author: Theuns, Sebastiaan
date: 2015-05-21
extension: .txt
mime: text/plain
words: 801
sentence: 28
flesch: 48
cache: cache/cord-001658-algzczs8.txt
txt: txt/cord-001658-algzczs8.txt
title:
  Complete Genome Sequence of a Porcine Epidemic Diarrhea Virus from a Novel Outbreak in Belgium, January 2015

summary:
  in gnotobiotic pigs Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States New variant of porcine epidemic diarrhea virus Comparison of porcine epidemic diarrhea viruses from Germany and the United States Trimmomatic: a flexible trimmer for Illumina sequence data Fast and accurate short read alignment with Burrows-Wheeler transform Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy A new coronavirus-like particle associated with diarrhea in swine Complete genome sequence of a highly prevalent isolate of porcine epidemic diarrhea virus in South China Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China Complete genome sequence of a novel porcine epidemic diarrhea virus in south China Distinct characteristics and complex evolution of PEDV strains Pathology of US porcine epidemic diarrhea virus strain PC21A


id: cord-014932-web2tdef
author: Li, Jian-qiang
title: Cloning the structure genes and expression the N gene of porcine epidemic diarrhea virus DX
date: 2009-05-28
extension: .txt
mime: text/plain
words: 1203
sentence: 42
flesch: 48
cache: cache/cord-014932-web2tdef.txt
txt: txt/cord-014932-web2tdef.txt
summary:
  The production of recombinant infectious DI-particles of a Sequence of the spike protein of the Porcine epidemic diarrhea virus Characterization of the structural proteins of porcine epidemic diarrhea virus, strain CV777 Cloning and sequence analysis of the M gene of Porcine epidemic diarrhea virus LJB/03 Cloning and sequence analysis of the N gene of porcine epidemic diarrhea virus LJB/03 Serologic study of the occurrence of epizootic viral diarrhea in swine in Switzerland Cloning and sequence analysis of the Korean strain of spike gene of Porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants Cloning and Sequence Analysis of the Nucleocapsid Gene of Porcine Epidemic Diarrhea Virus Chinju99 The molecular biology of coronaviruses Erhebungen uber porcine coronaviren in osterreich II porcine epidemic diarrhea virus (PEDV) der schweine Complete Genome Sequence of Transmissible Gastroenteritis Coronavirus PUR46-MAD Clone and Evolution of the Purdue Virus Cluster Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages A novel internal open reading frame product expressed from a polycistronic of porcine edpidemic diarrhea virus may not contribute to virus attenuation Disease of Swine A cell culture vaccine against bovine ephemeral fever Cloning and sequence analysis of the spike gene of Porcine epidemic diarrhea virus Chiju99 TheDX N protein contained 37 Arginine, 33 lysine and 36 Serine.


id: cord-299345-2i48ld8d
author: Nefedeva, Mariia
title: Molecular characteristics of a novel recombinant of porcine epidemic diarrhea virus
date: 2019-02-06
extension: .txt
mime: text/plain
words: 1536
sentence: 70
flesch: 40
cache: cache/cord-299345-2i48ld8d.txt
txt: txt/cord-299345-2i48ld8d.txt
summary:
  KC109141.1_PEDV_isolate_JS2008 JQ023162.1_PEDV_strain_aƩenuated_DR13_2003 JQ023161.1_PEDV_strain_virulent_DR13_1999 MF577027.1_PEDV_strain_PEDV/Belgorod/dom KX499468.1_TGEV_strain_TGEV_AHHF_2015 DQ201447.1_TGEV_strain_TS_2006 New variants of porcine epidemic diarrhea virus, China Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Genomic and epidemiological characteristics provide new insights into the phylogeographical and spatiotemporal spread of porcine epidemic diarrhea virus in Asia Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene New variant of porcine epidemic diarrhea virus Comparison of porcine epidemic diarrhea viruses from Germany and the United States Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus Distinct characteristics and complex evolution of PEDV strains Complete genome sequence of a porcine epidemic diarrhea virus RDP4: Detection and analysis of recombination patterns in virus genomes Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination SDT: a virus classification tool based on pairwise sequence alignment and identity calculation MUSCLE: multiple sequence alignment with high accuracy and high throughput Isolation and identification of porcine epidemic diarrhea virus in pigs under the outbreak at a large farm Biological characteristics of an epizootic isolate BS-08 of porcine epidemic diarrhea virus Porcine epidemic diarrhea virus and discovery of a recombinant swine enteric coronavirus New chimeric porcine coronavirus in swine feces Characterization of a novel chimeric swine enteric coronavirus from diseased pigs in Central Eastern Europe in 2016 Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China Acknowledgements We thank Olga Strizhakova for providing PEDV/ Belgorod/dom/2008.


id: cord-258546-1tf5ggfo
author: Chung, Hee-Chun
title: New emergence pattern with variant porcine epidemic diarrhea viruses, South Korea, 2012–2015
date: 2016-12-02
extension: .txt
mime: text/plain
words: 2074
sentence: 90
flesch: 55
cache: cache/cord-258546-1tf5ggfo.txt
txt: txt/cord-258546-1tf5ggfo.txt
summary:
  Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea ISolation of porcine epidemic diarrhea virus during outbreaks in South Korea Sequence of the spike protein of the porcine epidemic diarrhoea virus Coronavirus spike proteins in viral entry and pathogenesis Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs Isolation of porcine epidemic diarrhea virus (PEDV) in Korea Genetic characterization of porcine epidemic diarrhea virus in Korea from Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Suchard.


id: cord-016858-pbjj50bx
author: Jung, Kwonil
title: Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues
date: 2015-09-10
extension: .txt
mime: text/plain
words: 2136
sentence: 97
flesch: 45
cache: cache/cord-016858-pbjj50bx.txt
txt: txt/cord-016858-pbjj50bx.txt
summary:
  Due to the high sensitivity, specifi city, and convenience in using Alexa Fluor ® 488 in frozen tissues, this chapter details an IF staining method for the rapid and precise detection of PEDV antigens in PEDV-infected, frozen tissues, contributing to verifi cation of the tissue sites of PEDV replication in infected pigs. In IHC of frozen tissues where viral antigens are well preserved, the use of specific antibodies labeled with fluorescence dyes provides excellent advantages and convenience, resulting in high sensitivity and specificity of IHC and reduction of operation time.


id: cord-279903-z0wf1wli
author: Wang, Leyi
date: 2014-07-12
extension: .txt
mime: text/plain
words: 2382
sentence: 90
flesch: 52
cache: cache/cord-279903-z0wf1wli.txt
txt: txt/cord-279903-z0wf1wli.txt
title:
  Development and evaluation of a duplex real-time RT-PCR for detection and differentiation of virulent and variant strains of porcine epidemic diarrhea viruses from the United States

summary:
  Intra-specificity of the duplex RT-PCR assay was determined using single probe of either virulent PEDV probe for variant PEDV strain or variant PEDV probe for virulent PEDV strain and two probes for both types of PEDVs for positive control. In contract, the duplex RT-PCR did not cross-react with any other pig viruses used in the study, the Cy5 probe did not cross-react with variant PEDV strain, and the FAM probe did not cross-react with virulent PEDV strain (Table 2) .


id: cord-279794-hn5vmic0
author: Guo, Jiahui
title: Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains
date: 2018-08-27
extension: .txt
mime: text/plain
words: 2929
sentence: 106
flesch: 43
cache: cache/cord-279794-hn5vmic0.txt
txt: txt/cord-279794-hn5vmic0.txt
summary:
  Although increasing evidence suggests that PEDV routinely undergoes significant changes, especially in spike proteins (Lara-Romero et al., 2018; Stott et al., 2017) , the prevalence and evolution of PEDV strains is not well-defined and limited knowledge is known regarding the ways in which PEDV subgroups circulate among themselves and how they might influence the evolution of PEDV. These hot spot areas have the potential to be important reservoirs for the genetic variation of PEDVs, resulting in recombination between different PEDV subgroups.


id: cord-004713-gzts5h0y
author: Fennestad, K. L.
title: Pathogenetic observations on pleural effusion disease in rabbits
date: 1985
extension: .txt
mime: text/plain
words: 3603
sentence: 158
flesch: 49
cache: cache/cord-004713-gzts5h0y.txt
txt: txt/cord-004713-gzts5h0y.txt
summary:
  To examine viral growth and persistence of viraemia, serum was assayed for virus content at various times after rabbit infection with the two isolates ( Figs. 2 and 3 ). The findings, corrobated with other closely comparable observations, suggest that the emergence of PED as an intercurrent mortality problem during rabbit passage of pathogenicTreponema pallidum is the result of a specific selective pressure on a benign passenger virus.


id: cord-003587-zminzrov
author: Wang, Xueyu
date: 2019-04-15
extension: .txt
mime: text/plain
words: 3634
sentence: 146
flesch: 44
cache: cache/cord-003587-zminzrov.txt
txt: txt/cord-003587-zminzrov.txt
title:
  Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method

summary:
  PSR detection methods have been used for numerous human and veterinary pathogens This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay.


id: cord-290833-m0wodqr3
author: Yuan, Lvfeng
title: Synthetic surfactin analogues have improved anti-PEDV properties
date: 2019-04-11
extension: .txt
mime: text/plain
words: 3587
sentence: 155
flesch: 47
cache: cache/cord-290833-m0wodqr3.txt
txt: txt/cord-290833-m0wodqr3.txt
summary:
  The SLPs were tested for anti-PEDV activity using a plaque reduction assay. In order to explore the relationship between CMC, anti-PEDV activity, and hemolytic activity, scatter plots for pairs of assays (Fig 5) .


id: cord-010053-kniq2mbw
author: Lee, Sunhee
date: 2019-05-20
extension: .txt
mime: text/plain
words: 4072
sentence: 121
flesch: 39
cache: cache/cord-010053-kniq2mbw.txt
txt: txt/cord-010053-kniq2mbw.txt
title:
  Molecular characteristics and pathogenic assessment of porcine epidemic diarrhoea virus isolates from the 2018 endemic outbreaks on Jeju Island, South Korea

summary:
  An emerging and reemerging epizootic swine virus Porcine epidemic diarrhoea virus Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112 Reemergence of porcine epidemic diarrhea virus on Jeju Island Outbreak-related porcine epidemic diarrhea virus strains similar to US strains Complete genome sequence of a novel S-insertion variant of porcine epidemic diarrhea virus from South Korea Genomic and antigenic characterization of porcine epidemic diarrhoea virus strains isolated from South Korea Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene A simple method of estimating fifty percent endpoints Porcine reproductive and respiratory syndrome virus nucleocapsid protein modulates interferon-β production by inhibiting IRF3 activation in immortalized porcine alveolar macrophages Coronaviruses The neighbor-joining method: A new method for reconstructing phylogenetic trees Mutation in the cytoplasmic retrieval signal of porcine epidemic diarrhea virus spike (S) protein is responsible for enhanced fusion activity Emergence of porcine epidemic diarrhea virus in the United States: Clinical signs, lesions, and viral genomic sequences MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools Porcine epidemic diarrhea virus 3C-like protease regulates its interferon antagonism by cleaving NEMO An economic evaluation of intervention strategies for Porcine Epidemic Diarrhea (PED) Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1 Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation Identification and pathogenicity of a variant porcine epidemic diarrhea virus field strain with reduced virulence


id: cord-275499-25dp6u68
author: Tan, Zhen
title: Porcine Epidemic Diarrhea Altered Colonic Microbiota Communities in Suckling Piglets
date: 2019-12-30
extension: .txt
mime: text/plain
words: 4432
sentence: 187
flesch: 41
cache: cache/cord-275499-25dp6u68.txt
txt: txt/cord-275499-25dp6u68.txt
summary:
  Supplementary Materials: The following are available online at http://www.mdpi.com/2073-4425/11/1/44/s1, Figure S1 : Detection of PEDV in healthy groups and diarrhea groups using qPCR, Figure S2 : Shared OTU analysis of the different piglet groups using Venn diagrams, Figure S3 : Significant differences in microbe compositions between piglet groups, (* P < 0.05, ** P < 0.01).


id: cord-301347-22lt6h40
author: Jarvis, Matthew C.
date: 2016-01-01
extension: .txt
mime: text/plain
words: 4265
sentence: 149
flesch: 43
cache: cache/cord-301347-22lt6h40.txt
txt: txt/cord-301347-22lt6h40.txt
title:
  Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus

summary:
  The major clade of US PEDV strains was supported by high posterior probability (100%) and appears to have diverged further into two highly supported sublineages (99% and 100% posterior probability). The estimated tMRCA of the minor clade of US PEDV strains is July 2009-2011, and the estimated tMRCA of the major clade of US PEDV strains is September 2010-August 2012.


id: cord-290819-zhywlf6r
author: Wu, Jiaqi
date: 2020-07-27
extension: .txt
mime: text/plain
words: 4562
sentence: 197
flesch: 39
cache: cache/cord-290819-zhywlf6r.txt
txt: txt/cord-290819-zhywlf6r.txt
title:
  The antiviral protein viperin interacts with the viral N protein to inhibit proliferation of porcine epidemic diarrhea virus

summary:
  The antiviral protein viperin is a radical SAM enzyme Peroxisomes are signaling platforms for antiviral innate immunity Monkey viperin restricts porcine reproductive and respiratory syndrome virus replication Structural studies of viperin, an antiviral radical SAM enzyme Not just fat: the structure and function of the lipid droplet Cloning and sequence analysis of the N gene of porcine epidemic diarrhea virus LJB/03 Porcine epidemic diarrhea virus infection inhibits interferon signaling by targeted degradation of The role of viperin in the innate antiviral response In vivo and in vitro studies on the antiviral activities of viperin against influenza H1N1 virus infection Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs IFN-lambda preferably inhibits PEDV infection of porcine intestinal epithelial cells compared with IFN-alpha Immunological effects of different types of synthetic CpG oligodeoxynucleotides on porcine cells Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells Membrane curvature and mechanisms of dynamic cell membrane remodelling Type I interferons in infectious disease Contribution of the porcine aminopeptidase N (CD13) receptor density to porcine epidemic diarrhea virus infection Proteolytic activation of the porcine epidemic diarrhea coronavirus spike fusion protein by trypsin in cell culture Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Plasmacytoid dendritic cells: recent progress and open questions Antiviral protein viperin promotes toll-like receptor 7-and toll-like receptor 9-mediated type The cells were examined under an inverted fluorescence and phase-contrast microscope (Olympus) to determine the subcellular localization of viperin protein.


id: cord-272031-o2hx667i
author: Carvajal, Ana
title: Porcine epidemic diarrhoea: new insights into an old disease
date: 2015-09-29
extension: .txt
mime: text/plain
words: 4813
sentence: 190
flesch: 47
cache: cache/cord-272031-o2hx667i.txt
txt: txt/cord-272031-o2hx667i.txt
summary:
  Submit your next manuscript to BioMed Central and take full advantage of: Virus-like particles associated with porcine epidemic diarrhoea A new coronavirus-like particle associated with diarrhoea in swine Update on porcine epidemic diarrhoea Diseases of swine Porcine epidemic diarrhoea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis Evaluation of an ELISA for the detection of porcine epidemic diarrhea virus in feces of naturally infected pigs Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary Transmissible gastroenteritis and porcine epidemic diarrhoea in Britain ELISA detection of group a rotavirus, transmissible gastroenteritis virus and porcine epidemic diarrhoea virus in faeces of piglets An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy Porcine epidemic diarrhoea (PED) -Neuausbrüche in deutschen Mastschweinebeständen Comparison of porcine epidemic diarrhoea viruses from Germany and the United States Emergence of porcine epidemic diarrhea virus in southern Germany Surveillance and control of PED coronavirus in pig in Italy First case of porcine epidemic diarrhoea (PED) caused by a new variant of PED virus in the Netherlands Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in Belgium Complete Genome Sequence of a Porcine Epidemic Diarrhea S Gene Indel Strain Isolated in France in Study on the culture of porcine epidemic diarrhoea virus adapted to fetal porcine intestine primary cell monolayer An outbreak of swine diarrhoea of a new-type associated with coronavirus-like particles in Japan Isolation of porcine epidemic diarrhoea virus (PEDV) in Korea Porcine epidemic diarrhoea in Trang Province Chinese-like strain of porcine epidemic diarrhoea virus Genetic characterization of porcine epidemic diarrhoea virus (PEDV) isolates from southern Vietnam during 2009-2010 outbreaks Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines New variants of porcine epidemic diarrhea virus, China Outbreak of porcine epidemic diarrhoea in suckling piglets Emergence of porcine epidemic diarrhoea virus in the United States: clinical signs, lesions, and viral genomic sequences Scientific Opinion on porcine epidemic diarrhoea and emerging pig deltacoronavirus Discovery of seven novel mammalian and avian coronaviruses in Deltacoronavirus supports bat coronaviruses as the gene source of Alphacoronavirus and Betacoronavirus and avian coronaviruses as the gene source of Gammacoronavirus and Deltacoronavirus Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhoea virus and its antibodies Use of an internal control in a quantitative RT-PCR assay for quantitation of porcine epidemic diarrhoea virus shedding in pigs Pathogenesis of porcine epidemic diarrhoea virus isolate (US/Iowa/18984/ 2013) in 3-week-old weaned pigs Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs Multiple factors contribute to persistent porcine epidemic diarrhoea infection in the field: an investigation on porcine epidemic diarrhoea repeated outbreaks in the same Herd Pathology of US porcine epidemic diarrhoea virus strain PC21A Tissue tropism, shedding, carriage, antibody response, and aerosol transmission The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhoea virus in plasma Survivability of porcine epidemic diarrhoea virus (PEDV) in bovine plasma submitted to spray drying processing and held at different time by temperature storage conditions Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhoea in Canada Porcine epidemic diarrhoea virus RNA present in commercial spray-dried porcine plasma is not infectious to naïve pigs Analysis of spray dried porcine plasma (SDPP) produced in Brazil and western Canada confirm negative porcine epidemic diarrhoea virus (PEDV) status of pigs in these regions Experimental infection of pigs with a new porcine enteric coronavirus, CV-777 In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in the intestinal tissues from naturally intected piglets Porcine epidemic diarrhoea virus infects and replicates in porcine alveolar macrophages Porcine epidemic diarrhoea virus variants with high pathogenicity Distinct characteristics and complex evolution of PEDV strains A novel pathogenic mammalian orthoreovirus from diarrheic pigs and swine blood meal in the United States Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus Immunohistochemical detection of porcine epidemic diarrhoea virus compared to other methods Previous infection of sows with a mild strain of porcine epidemic diarrhea virus confers protection against infection with a severe strain Immunoprophylactic effect of chicken egg yolk immunoglobulin (IgY) against porcine epidemic diarrhea virus (PEDV) in piglets Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages Porcine epidemic diarrhea: a review of current epidemiology and available vaccines Molecular epidemiology of porcine epidemic diarrhoea virus in China An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration We gratefully acknowledge the pig farmers and veterinarians, their organisations and the feed companies for their active cooperation in the development of our research.


id: cord-266571-qbskh1uu
author: de Arriba, M.L
date: 2002-06-04
extension: .txt
mime: text/plain
words: 5107
sentence: 161
flesch: 34
cache: cache/cord-266571-qbskh1uu.txt
txt: txt/cord-266571-qbskh1uu.txt
title:
  Lymphoproliferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus

summary:
  After the challenge, there was an increase in the lymphocyte proliferative response in pigs from group 1, however this increase was not reflected either by an enhancement of the virus-specific antibody secreting cell response or the GMT of PEDV-specific serum IgG and IgA (De Arriba et al., 2001b) . Lymphocyte proliferation responses of transmissible gastroenteritis virus or porcine respiratory coronavirus Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus Evaluation of a bloking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhea virus and its antibodies Seroprevalence of porcine epidemic diarrhea virus infection among different types of breeding swine farms in Spain Revision of the taxonomy of the Coronavirus, Torovirus and Arterivirus genera Antibody-mediated protection of mucosal surfaces Isotype-specific antibody-secreting cells in systemic and mucosal associated lymphoid tissues and antibody responses in serum of conventional pigs inoculated with PEDV Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)


id: cord-255238-adpn5fb9
author: Pan, Yongfei
title: Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China
date: 2017-09-28
extension: .txt
mime: text/plain
words: 4524
sentence: 159
flesch: 47
cache: cache/cord-255238-adpn5fb9.txt
txt: txt/cord-255238-adpn5fb9.txt
summary:
  Structure homology-modeling of SeACoV S glycoprotein was performed by the SWISS-MODEL server (https://www.swissmodel.expasy.org/). The entire SeACoV S protein had 86.4% aa identical with S of the HKU2/GD430 strain, but there was only a 67.4% identity in the extreme NTD of the S protein (S-NTD) between SeACoV and HKU2.


id: cord-265679-7gzont7l
author: Guo, Nan
date: 2018-09-18
extension: .txt
mime: text/plain
words: 5268
sentence: 208
flesch: 46
cache: cache/cord-265679-7gzont7l.txt
txt: txt/cord-265679-7gzont7l.txt
title:
  Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus

summary:
  The number of virus infecting cells did not diminish. To better understand the inhibitory effects of Caerin1.1 against the progeny virus production of PEDV, we added Caerin1.1 at 1 hpi to PEDV infected cells.


id: cord-263439-oquk4t96
author: Park, Jung-Eun
title: Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells
date: 2014-10-13
extension: .txt
mime: text/plain
words: 5417
sentence: 201
flesch: 40
cache: cache/cord-263439-oquk4t96.txt
txt: txt/cord-263439-oquk4t96.txt
summary:
  To confirm the role of exogenous proteases in PEDV infection, PEDV infected cells were treated with trypsin and evaluated viral infectivity by immunocytochemistry. Both approaches provided similar conclusions on the mechanism of PEDV cell entry.


id: cord-273745-mwjh5se7
author: Meng, Fandan
date: 2014-03-25
extension: .txt
mime: text/plain
words: 5528
sentence: 186
flesch: 47
cache: cache/cord-273745-mwjh5se7.txt
txt: txt/cord-273745-mwjh5se7.txt
title:
  A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry

summary:
  However, when PEDV, TGEV and porcine pseudorabies virus were incubated with peptide H (HVTTTFAPPPPR), only infection of Vero cells by PEDV was inhibited. Immunofluoresence assays indicated that inhibition of PEDV infection by peptide H was independent of pAPN.


id: cord-279598-xzionafe
author: Chang, Chia-Yu
date: 2019-02-21
extension: .txt
mime: text/plain
words: 5407
sentence: 171
flesch: 42
cache: cache/cord-279598-xzionafe.txt
txt: txt/cord-279598-xzionafe.txt
title:
  Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhoea Virus Spike Protein

summary:
  As shown in Fig. 6 , the monomer of PEDV S protein was divided into S1 (a.a. 1-735, in light-blue) and S2 (a.a. 736-1378, in grey). A full-length homology model of the ectodomain of recombinant PEDV S protein was generated by SWISS-Model and built with ProMod3 version 1.0.0 (https://swissmodel.expasy. org).


id: cord-298685-qxkxjxsz
author: Pensaert, Maurice B.
title: Porcine epidemic diarrhea: A retrospect from Europe and matters of debate
date: 2016-12-02
extension: .txt
mime: text/plain
words: 5865
sentence: 201
flesch: 45
cache: cache/cord-298685-qxkxjxsz.txt
txt: txt/cord-298685-qxkxjxsz.txt
summary:
  Surveillance and control of PED coronavirus in pig in Italy Seqence determination of the nucleocapsid protein gene of the porceni epidemic diarhoea viris confirms that this virusis a coronavirus relatyed to human coronavirus 229 E and porcine transmissible gastroenteritis virus Enzyme-linked immunosorbent assay for the detection of the coronaviruslike agent and its antibodies in pigs with porcine epidemic diarrhea Prevalence of influenza-, Aujeszky, transmissible gastroenteritis and procine epizootic diarrhea virus in feeder pigs Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhea virus and its antibodies Porcine epidemic diarrhoea: new insights into an old disease Virus-like particles associated with porcine epidemic diarrhoea Pathogenesis comparison between the United States porcine epidemic diarrhoea virus prototype and S-INDEL-variant strains in conventional neonatal piglets Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection Pathology of experimental CV777 coronavirus enteritis in piglets Porcine epidemic diarrhea virus among farmed pigs Prevalence of porcine epidemic diarrhea (PED) virus in the pig population of different countries The pathogenesis of an enteric infection in pigs experimentally induced by the coronavirus-like agent CV 777 Experimental infection of pigs with a new porcine enteric coronavirus CV777 Scientific opinion on porcine epidemic diarrhea and emerging deltacoronavirus Complete genome sequence of a novel porcine epidemic diarrhea virus in south China Complete genome sequence of a porcine epidemic diarrhea S gene indel strain isolated in France Comparison of porcine epidemic diarrhoea viruses from Germany and the United States Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Experimental infection of piglets with a korean strain of porcine epidemic diarrhoea virus Genome sequencing and analysis of a novelrecombinant porcine epidemic diarrhea virus strain from Henan Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy Outbreak of porcine epidemic diarreha virus in Portugal Enterotoxigenic Eschirichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrohea in Hungary Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Letter to the editor. Pig Farm A new coronaviruslike particle associated with diarrhea in swine An immunoelectron and immunofluorescent study on the antigenic relationship between the coronavirus-like agent CV777 and several coronaviruses Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs Transmissible gastroenteritis and porcine epidemic diarrhoea in Britain Diagnosis and investigations on PED in northern Italy Differentiation betyween human coronaviruses NL63 and 229 E using a novel double-antibody sandwich enzyme-linked immunosorbent assay based on specific monoclonal antibodies Emergence of porcine epidemic diarrhea virus in southern Germany Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in Belgium First case of Porcine Epidemic Diarrhea (PED) caused by a new variant of PED virus in The Netherlands Prevalence of infections with enzootic respiratory and enteric viruses in feeder pigs entering fattening units Distinct characteristics and complex evolution of PEDV strains, north America Coronavirus pathogenesis and the emerging pathogen Severe Acute Respiratory Syndrome coronavirus.


id: cord-000699-2mfbqs8i
author: Sun, Rui-Qin
title: Outbreak of Porcine Epidemic Diarrhea in Suckling Piglets, China
date: 2012-01-17
extension: .txt
mime: text/plain
words: 1419
sentence: 54
flesch: 50
cache: cache/cord-000699-2mfbqs8i.txt
txt: txt/cord-000699-2mfbqs8i.txt
summary:
  Human pathogenic hantaviruses and prevention of infection Hantavirus in African wood mouse, Guinea Novel hantavirus sequences in shrew, Guinea Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior Bats: important reservoir hosts of emerging viruses Molecular phylogenetics and the origins of placental mammals Genomic characterization of M and S RNA segments of hantaviruses isolated from bats Hantavirus-induced immunity in rodent reservoirs and humans First human case of haemorrhagic fever with renal syndrome in the Central African Republic Serological evidence of human hantavirus infections in Guinea, West Africa Chinese-like strain of porcine epidemic diarrhea virus Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR Critical epitopes in transmissible gastroenteritis virus neutralization Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic virus diarrhoea (EVD): comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections Large-scale spatial-transmission models of infectious disease Identifi cation of the membrane protein of porcine epidemic diarrhea virus Coronavirus-like particles associated with diarrhea in baby pigs in Quebec We characterized the genetic variation of the PED virus (PEDV) that caused a large-scale outbreak in China during 2010-2011 and compared it with viruses in other outbreaks.


id: cord-255607-dbexsugq
author: Wu, Yang
date: 2020-05-31
extension: .txt
mime: text/plain
words: 6424
sentence: 241
flesch: 39
cache: cache/cord-255607-dbexsugq.txt
txt: txt/cord-255607-dbexsugq.txt
title:
  Porcine Epidemic Diarrhea Virus nsp15 Antagonizes Interferon Signaling by RNA Degradation of TBK1 and IRF3

summary:
  Briefly, nuclease reactions contained 4 µg of purified wild-type PEDV nsp15 protein, PEDV nsp15 mutant protein, or GST tag protein as control, and 6 µg TBK1 or IRF3 RNA transcribed and purified in vitro. The number below the red residue indicates its corresponding position at PEDV nsp15 protein, respectively.


id: cord-280564-kgoczioe
author: Conceição-Neto, Nádia
date: 2017-09-08
extension: .txt
mime: text/plain
words: 6208
sentence: 221
flesch: 44
cache: cache/cord-280564-kgoczioe.txt
txt: txt/cord-280564-kgoczioe.txt
title:
  Identification of an enterovirus recombinant with a torovirus-like gene insertion during a diarrhea outbreak in fattening pigs

summary:
  Although PEDV was detected, its low abundance indicated that other viruses were involved in the outbreak. Given the low abundance of PEDV in the fecal sample, and the large number of other viruses which can also cause gastro-intestinal disease in pigs, it was questioned in the present study if other viruses might have been present in the pig's fecal virome and if they could have contributed to the emergence of diarrhea on this farm.


id: cord-259794-6qoksn00
author: Shi, Da
date: 2017-01-03
extension: .txt
mime: text/plain
words: 7096
sentence: 281
flesch: 42
cache: cache/cord-259794-6qoksn00.txt
txt: txt/cord-259794-6qoksn00.txt
title:
  Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage, Enhancing Cell Survival, and is Involved in PEDV Growth

summary:
  Moreover, the interaction of N protein with porcine NPM1 protein also was validated in our studies (Fig. 2F ). In total, these data indicated that N protein is able to interact with NPM1 protein.


id: cord-254192-86ksgl5t
author: Li, Liang
date: 2019-10-17
extension: .txt
mime: text/plain
words: 7276
sentence: 240
flesch: 42
cache: cache/cord-254192-86ksgl5t.txt
txt: txt/cord-254192-86ksgl5t.txt
title:
  IFN-Lambda 3 Mediates Antiviral Protection Against Porcine Epidemic Diarrhea Virus by Inducing a Distinct Antiviral Transcript Profile in Porcine Intestinal Epithelia

summary:
  The IFN family is categorized into three different types: type I IFN (IFN-α/β), type II IFN (IFN-γ), and type III IFN (IFN-λ). Mice with type I IFN or III IFN receptor knockout experience more severe viral intestinal infections, but Ifnl −/− mice show higher viral loads and more serious clinical symptoms than IFNAR −/− mice (17, 18) .


id: cord-292958-k5d5fo3i
author: Sekhon, Simranjeet Singh
title: Porcine epidemic diarrhea (PED) infection, diagnosis and vaccination: A mini review
date: 2017-01-04
extension: .txt
mime: text/plain
words: 7442
sentence: 211
flesch: 31
cache: cache/cord-292958-k5d5fo3i.txt
txt: txt/cord-292958-k5d5fo3i.txt
summary:
  This sequence is particularly rich in arginine (Arg), serine (Ser) and asparagine (Asp) residues, and presents in PED virus N protein with no counterpart in the remainder of Coronaviruses. The encoded polypeptides range of PEDV N protein is from 377 to 455 amino acids and has the similar physical properties with the other members of the family Coronaviridae 60 .


id: cord-266660-0wq77k6y
author: Choi, Jong-Chul
date: 2014-06-19
extension: .txt
mime: text/plain
words: 1998
sentence: 89
flesch: 51
cache: cache/cord-266660-0wq77k6y.txt
txt: txt/cord-266660-0wq77k6y.txt
title:
  Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea

summary:
  Korean PEDV strains are denoted using bold characters. Nucleotide sequence alignment and phylogenetic tree analysis for complete genomes of PEDV strains.


id: cord-254405-yc1q20fz
author: Jie, Tao
date: 2018-07-31
extension: .txt
mime: text/plain
words: 3456
sentence: 141
flesch: 51
cache: cache/cord-254405-yc1q20fz.txt
txt: txt/cord-254405-yc1q20fz.txt
title:
  Preparation and characterization of an attenuated porcine epidemic diarrhea virus strain by serial passaging

summary:
  The results should help our Shanghai Agriculture Applied Technology Development Program of China (No. T20170111), and the Youth Talent Development Plan of Shanghai Municipal Agricultural System of China Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis New variant of porcine epidemic diarrhea virus, United States Investigation into the role of potentially contaminated feed as a source of the firstdetected outbreaks of porcine epidemic diarrhea in Canada Emergence of porcine epidemic diarrhea virus in southern Germany New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs New variants of porcine epidemic diarrhea virus, China Molecular characterization of the ORF3 and S1 genes of porcine epidemic diarrhea virus non S-INDEL strains in seven regions of China Complete genome sequence of PEDV from an outbreak in a vaccinated farm in Shandong Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China The identification and characterization of two novel epitopes on the nucleocapsid protein of the porcine epidemic diarrhea virus Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review Comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus Porcine epidemic diarrhea virus (PEDV) 3C-like protease-mediated nucleocapsid processing: a possible link to viral cell-culture adaptability Isolation and characterization of a variant porcine epidemic diarrhea virus in China Genetic variability and phylogeny of current Chinese porcine epidemic diarrhea virus strains based on spike, ORF3, and membrane genes Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays Differentiation of a vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Epidemiological survey on porcine diarrhea in swine breeding farms in China during 2013-2015 Molecular epidemiology of porcine epidemic diarrhea virus in China Environmental persistence of porcine coronaviruses in feed and feed ingredients Epidemiological survey of porcine epidemic diarrhea virus in 2014 Furthermore, we cultured the virus and screened for attenuated PEDV strains by analyzing the genovariations that occurred during passaging -confirming the results in animal experiments.


id: cord-260107-gqbtkf0x
author: Lee, Sunhee
title: Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112
date: 2015-10-02
extension: .txt
mime: text/plain
words: 7710
sentence: 262
flesch: 46
cache: cache/cord-260107-gqbtkf0x.txt
txt: txt/cord-260107-gqbtkf0x.txt
summary:
  All original feces and passaged PEDV viruses through passages 30 were classified into subgroup 2b along with the recent Korean field isolates, which were most closely clustered together with the emergent US strains in an adjacent clade with the same subgroup. Animal studies showed that KNU-141112 virus causes severe diarrhea and vomiting, fecal shedding, and acute atrophic enteritis, indicating that strain KNU-141112 is highly enteropathogenic in the natural host.


id: cord-003899-a4w2nnos
author: Yang, Jiwen
date: 2019-08-29
extension: .txt
mime: text/plain
words: 3817
sentence: 170
flesch: 42
cache: cache/cord-003899-a4w2nnos.txt
txt: txt/cord-003899-a4w2nnos.txt
title:
  Dietary 25-Hydroxyvitamin D(3) Supplementation Alleviates Porcine Epidemic Diarrhea Virus Infection by Improving Intestinal Structure and Immune Response in Weaned Pigs

summary:
  Porcine epidemic diarrhea virus induces caspase-independent apoptosis through activation of mitochondrial apoptosis-inducing factor Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis Effects of porcine epidemic diarrhea virus infection on nursery pig intestinal function and barrier integrity Immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (Gn) pigs infected with porcine epidemic diarrhea virus (PEDV) and serum cytokine responses of Gn pigs to acute PEDV infection Differential gene modulation of pattern-recognition receptor TLR and RIG-I-like and downstream mediators on intestinal mucosa of pigs infected with PEDV non S-INDEL and PEDV S-INDEL strains Outbreak of porcine epidemic diarrhea in suckling piglets Porcine epidemic diarrhea virus: We found that high dose 25-hydroxyvitamin D(3) supplementation could ease intestinal injury and inhibit intestinal immune response induced by porcine epidemic diarrhea virus (PEDV), suggesting that feeding a high dose of 25-hydroxyvitamin D(3) could be used as an approach against PEDV infection.


id: cord-003328-vvle1q1e
author: Altawaty, Tawfeek
title: Lack of LTβR Increases Susceptibility of IPEC-J2 Cells to Porcine Epidemic Diarrhea Virus
date: 2018-11-21
extension: .txt
mime: text/plain
words: 4128
sentence: 153
flesch: 44
cache: cache/cord-003328-vvle1q1e.txt
txt: txt/cord-003328-vvle1q1e.txt
summary:
  Specifically, many more LTβR null cells than LTβR +/+ cells exhibited increased cell volume (vertical axis) and decreased cell membrane thickness (horizontal axis) To examine cell proliferation, LTβR +/+ and LTβR −/− cells were plated in 96-well plates at 5 × 10 3 cells per well in 100 µL cell culture medium and maintained at 37 • C in a humidified incubator containing 5% CO 2 .


id: cord-254317-n2knqj4z
author: Su, Yunfang
title: The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets
date: 2018-11-27
extension: .txt
mime: text/plain
words: 8209
sentence: 333
flesch: 57
cache: cache/cord-254317-n2knqj4z.txt
txt: txt/cord-254317-n2knqj4z.txt
summary:
  By IF staining for the PEDV S1 proteins, a few PEDV antigens were observed in the jejunum of the icPC22A-S1Δ197-inoculated piglets (green alone, positive for S1Δ197 only), while many antigens were observed in those of the icPC22 A-inoculated piglets [yellow, representing positive for both S1Δ197 (green) and S1°d omain (red)] and the coinfection piglets (yellow) (Fig. 2C) . PEDV S protein mediates the essential functions of receptor binding (via S1 subunit) and subsequent fusion of the viral and cellular membranes (via S2 subunit) (Li, 2016) .


id: cord-004727-9sniu39j
author: Fennestad, K. L.
title: Pleural effusion disease in rabbits: Observations on viraemia, immunity and transmissibility
date: 1981
extension: .txt
mime: text/plain
words: 3049
sentence: 147
flesch: 52
cache: cache/cord-004727-9sniu39j.txt
txt: txt/cord-004727-9sniu39j.txt
summary:
  Accepting only these four deaths as caused by PEDV, the time of death was considerably delayed in baby rabbits as compared with older animals. The lack of cross-infection by contact between baby rabbits before and after weaning indicates that direct transmission of PED is probably a rare occurrence.


id: cord-003973-pnareltx
author: Hulst, M.M.
date: 2019-04-01
extension: .txt
mime: text/plain
words: 9521
sentence: 300
flesch: 51
cache: cache/cord-003973-pnareltx.txt
txt: txt/cord-003973-pnareltx.txt
title:
  Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma

summary:
  For PEDV virus isolation in 175 cm 2 flasks the denominator in this RF max formula was corrected for the volume or grams tested, and for SDPP powders the nominator multiplied with 6Á7 as described above. For preparation of PEDV virus stocks and virus isolation assays Vero cells were grown in serum-free medium, i.e. 1 : 1 mixture of EMEM and UltraMCDK (Lonza Group Ltd, Bazel, Switzerland) supplemented with 10 lg ml À1 trypsin and 1% v/v anti-anti.


id: cord-273705-0oyzg5tq
author: Duffy, Mark A
date: 2018-08-29
extension: .txt
mime: text/plain
words: 4636
sentence: 164
flesch: 51
cache: cache/cord-273705-0oyzg5tq.txt
txt: txt/cord-273705-0oyzg5tq.txt
title:
  Impact of dietary spray-dried bovine plasma addition on pigs infected with porcine epidemic diarrhea virus

summary:
  The average fecal PEDV RNA shedding time was 7.2 ± 1.0 d for the PEDV–BovSDP group and 9.3 ± 1.1 d for the PEDV group with an overall time to clearance of PEDV shedding of 11 d for PEDV–BovSDP pigs and at least 14 d for PEDV pigs, which was not different (P = 0.215). Overall, there were numerical differences among groups for the ADG: NEG pigs had the highest ADG, and PEDV pigs had the lowest ADG; however, the differences were not significant.


id: cord-279784-o80x8nj7
author: Wu, Yu
date: 2019-10-28
extension: .txt
mime: text/plain
words: 4396
sentence: 211
flesch: 52
cache: cache/cord-279784-o80x8nj7.txt
txt: txt/cord-279784-o80x8nj7.txt
title:
  Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain

summary:
  Porcine epidemic diarrhea virus: an emerging and reemerging epizootic swine virus Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge Novel porcine epidemic diarrhea virus variant with large genomic deletion Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate Development of a live attenuated vaccine candidate against duck Tembusu viral disease Genome organization of porcine epidemic diarrhea virus New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs Isolation and molecular characterization of porcine epidemic diarrhea viruses collected in Japan in 2014 Virus-like particles associated with porcine epidemic diarrhea New variants of porcine epidemic diarrhea cirus, China Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan Porcine epidemic diarrhea vaccine evaluation using a newly isolated strain from Korea New emergence pattern with variant porcine epidemic diarrhea viruses Mucosal and systemic isotypespecific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses Genetic epidemiology of porcine epidemic diarrhea virus circulating in China in 2012-2017 based on spike gene Trypsin-independent porcine epidemic diarrhea virus US strain with altered virus entry mechanism Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays Isolation and characterization of a variant porcine epidemic diarrhea virus in China A highly pathogenic strain of porcine deltacoronavirus caused watery diarrhea in newborn piglets Oral administration of coated PEDV-loaded microspheres elicited PEDV-specific immunity in weaned piglets Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA Comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus PEDV ORF3 encodes an ion channel protein and regulates virus production Preparation and characterization of an attenuated porcine epidemic diarrhea virus strain by serial passaging Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Porcine epidemic diarrhea (PED) is an acute and highly infectious intestinal disease in pigs; it is caused by porcine epidemic diarrhea virus (PEDV), which has caused enormous economic losses to the global pig industry


id: cord-261036-zdhg4axx
author: Shirato, Kazuya
title: Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice
date: 2010-09-09
extension: .txt
mime: text/plain
words: 3339
sentence: 143
flesch: 56
cache: cache/cord-261036-zdhg4axx.txt
txt: txt/cord-261036-zdhg4axx.txt
summary:
  In our study, both mouse-adapted and non-adapted MK strains of PEDV could infect and grow in suckling mouse brains, suggesting that a receptor molecule utilized by both viruses exists in suckling mouse brain. In the present study, we isolated a strain of PEDV that was more virulent than the original tissue-culture-adapted virus after passage through mouse brain cells.


id: cord-257136-zpeh8pmc
author: Huang, Xin
date: 2019-04-26
extension: .txt
mime: text/plain
words: 3980
sentence: 149
flesch: 46
cache: cache/cord-257136-zpeh8pmc.txt
txt: txt/cord-257136-zpeh8pmc.txt
title:
  A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses

summary:
  To develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential diagnosis on swine enteric coronaviruses, specific primers and probes were designed based on the highly conserved regions of transmissible gastroenteritis virus (TGEV) N, porcine epidemic diarrhea virus (PEDV) M, porcine deltacoronavirus (PDCoV) M, and porcine enteric alphacoronavirus (PEAV) N genes respectively. This well-established multiplex real-time RT-qPCR assay provided a rapid, efficient, specific, and sensitive tool for detection of swine enteric coronaviruses.


id: cord-009526-ghm1hvei
author: Bertolini, F.
title: Genomic investigation of piglet resilience following porcine epidemic diarrhea outbreaks
date: 2016-12-12
extension: .txt
mime: text/plain
words: 2791
sentence: 89
flesch: 40
cache: cache/cord-009526-ghm1hvei.txt
txt: txt/cord-009526-ghm1hvei.txt
summary:
  Fast model-based estimation of ancestry in unrelated individuals Evidence of infectivity of airborne porcine epidemic diarrhea virus and detection of airborne viral RNA at long distances from infected herds Tolerance of infections Rapid and accurate haplotype phasing and missing data inference for whole genome association studies using localized haplotype clustering ENRICHR: interactive and collaborative HTML5 gene list enrichment analysis tool The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, Cv-777 Pathology of experimental CV777 coronavirus enteritis in piglets II: electron microscopic study Discovering motifs in ranked lists of DNA sequences GORILLA: a tool for discovery and visualization of enriched GO terms in ranked gene lists Intestinal ion transport and the pathophysiology of diarrhea The human disease network Principles of selective ion transport in channels and pumps Viral perturbations of host networks reflect disease etiology Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs Efficient mapping of mendelian traits in dogs through genome-wide association Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus Mechanisms of porcine diarrheal disease Contribution of the porcine aminopeptidase N (CD13) receptor density to porcine epidemic diarrhea virus infection Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to na€ ıve pigs A new coronavirus-like particle associated with diarrhea in swine PLINK: a toolset for whole-genome association and population-based linkage analysis Large-scale spatial-transmission models of infectious disease Whole-genome resequencing reveals loci under selection during chicken domestication Two ways to survive infection: what resistance and tolerance can teach us about treating infectious diseases Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages PEDv Update Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Coronavirus-like particles associated with diarrhea in baby pigs in Quebec Identification of the membrane protein of porcine epidemic diarrhea virus Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus Edgetic perturbation models of human inherited disorders The authors appreciate the financial support of the US Pig Genome Coordination Program, particularly Chris Tuggle and Cathy Ernst, and the State of Iowa.


id: cord-252772-f3fctcru
author: Wang, Changlin
title: The Coronavirus PEDV Evades Type III Interferon Response Through the miR-30c-5p/SOCS1 Axis
date: 2020-05-22
extension: .txt
mime: text/plain
words: 4965
sentence: 187
flesch: 44
cache: cache/cord-252772-f3fctcru.txt
txt: txt/cord-252772-f3fctcru.txt
summary:
  Compared with a mock uninfected control, PEDV did not increase the expression of IFNλ transcripts as observed until 12 hpi, and then gradually induced IFN-λ expression, indicating that PEDV infection elicits type III IFN expression at the late stage of infection instead of the early stage of infection in the Vero E6 cells (Figure 1A) , which was consistent with the results in porcine enteroids (Li et al., 2019) . PEDV suppresses IRF1-mediated type III IFN responses by reducing the number of peroxisomes and counteracting type III IFN response by PEDV nsp15 endoribonuclease Deng et al., 2019) .


id: cord-259324-g8kv4pvq
author: Ko, Suk-Min
title: Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor
date: 2011-10-28
extension: .txt
mime: text/plain
words: 2336
sentence: 86
flesch: 42
cache: cache/cord-259324-g8kv4pvq.txt
txt: txt/cord-259324-g8kv4pvq.txt
summary:
  First, to determine the appropriate conditions for selecting Lemna transformants, the effect of increasing concentrations of kanamycin was assessed on Lemna fronds (Fig. 1A) . (1998) reported that proliferation of Lemna fronds varied six-fold across the 25 geographic isolates of Lemna collected at various geographical locations around the world.


id: cord-273712-r2akpce8
author: Wang, Jingjing
date: 2016-02-19
extension: .txt
mime: text/plain
words: 2590
sentence: 112
flesch: 49
cache: cache/cord-273712-r2akpce8.txt
txt: txt/cord-273712-r2akpce8.txt
title:
  Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses

summary:
  Infection of lentiviruses pseudotyped shows that S proteins from different CoVs have varied abilities to mediate pseudotyped virus infection in different cell types from different tissues. key: cord-273712-r2akpce8 authors: Wang, Jingjing; Deng, Feng; Ye, Gang; Dong, Wanyu; Zheng, Anjun; He, Qigai; Peng, Guiqing title: Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses date: 2016-02-19 journal:


id: cord-033488-du8heorx
author: Ho, Thuong Thi
date: 2020-09-16
extension: .txt
mime: text/plain
words: 5443
sentence: 191
flesch: 39
cache: cache/cord-033488-du8heorx.txt
txt: txt/cord-033488-du8heorx.txt
title:
  Plant-Derived Trimeric CO-26K-Equivalent Epitope Induced Neutralizing Antibodies Against Porcine Epidemic Diarrhea Virus

summary:
  One of the possible reasons for the increase in the molecular weight of COE protein is that the N-glycosylation sites located within the COE-S protein of PEDV at amino acids 511 and 533 may influence the electrophoretic behavior during the PAGE separation (16, 17) . The COE-GCN4pII protein expression level in the leaf crude extract was semi-quantified via Western blotting by comparison of the Western blot signal intensities and The COE sequence encoding the COE protein of PEDV was fused with the C-terminal trimeric motif (GCN4pII) for generating trimeric COE protein.


id: cord-282466-r2sjv9ih
author: Antas, Marta
title: Current Status of Porcine Epidemic Diarrhoea (PED) in European Pigs
date: 2019-10-24
extension: .txt
mime: text/plain
words: 3325
sentence: 136
flesch: 48
cache: cache/cord-282466-r2sjv9ih.txt
txt: txt/cord-282466-r2sjv9ih.txt
summary:
  Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus Pathogenesis comparison between the United States porcine epidemic diarrhoea virus prototype and S-INDEL-variant strains in conventional neonatal piglets Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Scientific opinion on porcine epidemic diarrhea and emerging porcine deltacoronavirus Coronaviruses: an overview of their replication and pathogenesis Porcine epidemic diarrhea virus in North America Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains Immunohistochemical detection of porcine epidemic diarrhoea virus compared to other methods Porcine epidemic diarrhea in Europe: indetail analyses of disease dynamics and molecular epidemiology Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus Aminopeptidase-Nindependent entry of porcine epidemic diarrhea virus into Vero or porcine small intestine epithelial cells Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis, and immunoprophylaxis Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Porcine epidemic diarrhea virus: an emerging and reemerging epizootic swine virus Porcine epidemic diarrhea virus: current insights Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy Emerging and reemerging diseases in Asia and the Pacific with special emphasis on porcine epidemic diarrhoea Porcine epidemic diarrhea (PED) in Europe and strategies to control outbreaks Porcine epidemic diarrhea: a retrospect from Europe and matters of debate Variations in the detection of anti-PEDV antibodies in serum samples using three diagnostic testsshort communication Diagnostic notes update on porcine epidemic diarrhea Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; use of NGS to analyse genomic and sub-genomic RNAs Coronaviruses. in: Diseases of swine Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility Porcine epidemic diarrhea virus: a comprehensive review of molecular epidemiology, diagnosis and vaccines Quercetin 7-rhamnoside reduces porcine epidemic diarrhea virus replication via independent pathway of viral induced reactive oxygen species Investigation of three outbreaks of porcine epidemic diarrhea in Germany in 2016 demonstrates age dependent differences in the development of humoral immune response A retrospective study detects a novel variant of porcine epidemic diarrhea virus in England in archived material from the year Factors associated with farm-level infection of porcine epidemic diarrhea during the early phase of the epidemic in Japan in Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of Alphacoronavirus and Betacoronavirus and avian coronaviruses as the gene source of Gammacoronavirus and Deltacoronavirus Porcine epidemic diarrhea virus variants with high pathogenicity Evolutionary insights into the ecology of coronaviruses Distinct characteristics and complex evolution of PEDV strains Molecular characteristics of the spike gene of porcine epidemic diarrhoea virus strains in Eastern China in 2016


id: cord-272480-276r1lh7
author: Wang, Peng
date: 2019-02-09
extension: .txt
mime: text/plain
words: 2592
sentence: 125
flesch: 55
cache: cache/cord-272480-276r1lh7.txt
txt: txt/cord-272480-276r1lh7.txt
title:
  Isolation and recombinant analysis of variants of porcine epidemic diarrhea virus strains from Beijing, China

summary:
  There is evidence that the S gene of epidemic PEDV strains and genome of transmissible gastroenteritis virus (TEGV) strains have recombined when both were present in the same host to form a new recombinant porcine coronavirus, SeCoV [30, 31] . The sequence identity of PEDV strains showed that these strains are most similar to these strains LZW, CH/JX-1/2013, USAIllinois972013, USAKansas1252014, CH/GDZQ/2014, SHQPYM2013, AJ1102, CHZMDZY11, KoreaK14JB01, and CHYJ130330, respectively.


id: cord-292734-2g2ym81n
author: Jung, Kwonil
date: 2008-06-30
extension: .txt
mime: text/plain
words: 3254
sentence: 119
flesch: 51
cache: cache/cord-292734-2g2ym81n.txt
txt: txt/cord-292734-2g2ym81n.txt
title:
  Impact of porcine group A rotavirus co-infection on porcine epidemic diarrhea virus pathogenicity in piglets

summary:
  No significant differences in mean VH:CD ratio or clinical symptoms were observed between PEDV/PGAR and PEDV group piglets at 1, 2 and 3 DPI; Table 1 Diarrhea and detection of porcine epidemic diarrhea virus (PEDV) and porcine group A rotavirus (PGAR) by RT-PCR and virus isolation in the fecal samples from piglets infected with PEDV, PEDV/PGAR, or PGAR, or in control piglets at different days post-inoculation (DPI) Group DPI Diarrhea RT-PCR Virus isolation 1 2 3 1 2 3 1 2 3 PEDV 3/3 a 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 PEDV, PGAR 3/3 3/3 3/3 3, 2 b /3, 3 3, 3 b /3, 3 3, 3 b /3, 3 3, 2 c /3, 3 3, 3 c /3, 3 3, 3 c /3, 3 PGAR 0/3 2/3 3/3 0/3 2/3 3/3 0/3 2/3 3/3 Control 0/3 0/3 0/3 0/3 0/3 0/3 0/3 0/3 0/3 a Number of positive pigs/number of pigs tested. For the PEDV/PGAR group, the piglets were infected orally with PEDV at 12 h after inoculation with PGAR so that PEDV would replicate in intestinal epithelial cells with an established PGAR infection and at the time, PGAR group piglets were inoculated with PGAR.


id: cord-287913-pe6ot11q
author: Jung, Kwonil
date: 2007-06-18
extension: .txt
mime: text/plain
words: 2142
sentence: 74
flesch: 40
cache: cache/cord-287913-pe6ot11q.txt
txt: txt/cord-287913-pe6ot11q.txt
title:
  Effects of epidermal growth factor on atrophic enteritis in piglets induced by experimental porcine epidemic diarrhoea virus

summary:
  Histologically, the ratio of villous height:crypt depth of PEDV + EGF piglets was significantly higher than PEDV only piglets at 36 and 48 hpi. However, from 36 to 60 hpi, the Ki67 scores of PEDV + EGF piglets were significantly higher than PEDV only piglets (P < 0.05) ( Table 2 ; Fig. 1 ).


id: cord-255862-84u3c33m
author: Kim, Ji Won
title: Antiviral escin derivatives from the seeds of Aesculus turbinata Blume (Japanese horse chestnut)
date: 2017-07-01
extension: .txt
mime: text/plain
words: 2855
sentence: 120
flesch: 53
cache: cache/cord-255862-84u3c33m.txt
txt: txt/cord-255862-84u3c33m.txt
summary:
  In this research, we investigated whether escin derivatives 1–7 (including new compounds 2, 3, 5 and 6), without the angeloyl or tigloyl groups and with modified glycosidic linkages by hydrolysis, have PEDV inhibitory effects with less cytotoxicity. When the inhibitory effect of compound 4 was analyzed in detail at the concentrations (B) CPE inhibition assay of the n-BuOH fraction and the reaction fraction at concentrations of 1, 2, 5, and 10 lg/mL. Up to 2 lg/mL, the n-BuOH fraction and the reaction fraction from a two-step hydrolysis showed similar activities, but at high concentrations, the n-BuOH fraction showed cytotoxic effects and the reaction fraction had PEDV inhibitory effects in dose-dependent manner.


id: cord-277194-mj85mpo2
author: Perri, Amanda M.
date: 2019-05-08
extension: .txt
mime: text/plain
words: 4556
sentence: 170
flesch: 48
cache: cache/cord-277194-mj85mpo2.txt
txt: txt/cord-277194-mj85mpo2.txt
title:
  Factors Associated With Time to Elimination of Porcine Epidemic Diarrhea Virus in Individual Ontario Swine Herds Based on Surveillance Data

summary:
  However, despite these limitations, this study provided novel insight in regards to PEDV elimination times in Ontario. Such data allowed for the determination of the duration of PEDV infection in individual herds based on herd type, year and season of diagnosis.


id: cord-276989-441aclcc
author: Liu, Jianbo
date: 2020-03-12
extension: .txt
mime: text/plain
words: 4026
sentence: 151
flesch: 46
cache: cache/cord-276989-441aclcc.txt
txt: txt/cord-276989-441aclcc.txt
title:
  Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum

summary:
  Validation of specificity and sensitivity of the immunochromatographic strip test PEDV SIgA positive colostrum, TGEV SIgA positive colostrum, PoRV SIgA positive colostrum, and PEDV SIgA negative colostrum were used to test the specificity of the strip. When compared with enzyme-linked immunosorbent assay, kappa value suggesting that the strip could be used to detect PEDV specific SIgA in colostrum samples.


id: cord-261993-u2a26brw
author: Kim, Yonghyan
title: Stability of Porcine Epidemic Diarrhea Virus on Fomite Materials at Different Temperatures
date: 2018-02-13
extension: .txt
mime: text/plain
words: 3886
sentence: 147
flesch: 46
cache: cache/cord-261993-u2a26brw.txt
txt: txt/cord-261993-u2a26brw.txt
summary:
  Separate fomite samples were used at every time point, and eluates were processed independently for quantification of infectious virus and viral RNA. Decay of infectious virus was rapid at RT but delayed when stored at 4 °C.


id: cord-284322-synuzaxm
author: Borel, Nicole
date: 2010-07-27
extension: .txt
mime: text/plain
words: 5451
sentence: 220
flesch: 41
cache: cache/cord-284322-synuzaxm.txt
txt: txt/cord-284322-synuzaxm.txt
title:
  Mixed infections with Chlamydia and porcine epidemic diarrhea virus - a new in vitro model of chlamydial persistence

summary:
  If Chlamydia pecorum infection might induce a down regulation of the host PEDV receptor needed for syncytium formation at 14-15 hours post-chlamydial infection, this could produce a reduction in syncytium formation without reducing viral entry or replication -the possible persistence inducer mechanism. In other words, Chlamydia pecorum inclusions Vero cells were infected with Chlamydia abortus with subsequent PEDV inoculation and stained as with an anti-Chlamydia antibody and DAPI; c) Frequency of inclusions with various sizes was calculated and mono and double infected cells were compared according to the inclusion size.


id: cord-301655-6nxhvvm4
author: Lei, Xi-Mei
date: 2019-08-10
extension: .txt
mime: text/plain
words: 6457
sentence: 207
flesch: 40
cache: cache/cord-301655-6nxhvvm4.txt
txt: txt/cord-301655-6nxhvvm4.txt
title:
  Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers

summary:
  The purified recombinant PEDV S1, ORF3C, E, ADRP, Nsp1, Nsp2, and Ac peptides were resolved on a 5-10% Bis-Tris polyacrylamide gel (Invitrogen) by electrophoresis and subsequently transferred onto a polyvinylidene difluoride (PVDF) membrane. Collectively, the data indicated that the PEDV S1 protein was more specific and accurate as a detection antigen in ELISA tests.


id: cord-286831-ni7qfjk9
author: Choi, Hwa-Jung
title: Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus
date: 2008-11-06
extension: .txt
mime: text/plain
words: 3839
sentence: 145
flesch: 49
cache: cache/cord-286831-ni7qfjk9.txt
txt: txt/cord-286831-ni7qfjk9.txt
summary:
  key: cord-286831-ni7qfjk9 authors: Choi, Hwa-Jung; Kim, Jin-Hee; Lee, Choong-Hwan; Ahn, Young-Joon; Song, Jae-Hyoung; Baek, Seung-Hwa; Kwon, Dur-Han title: Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus date: 2008-11-06 journal: Although a variety of pharmacological activities associated with chemicals in H. cordata has been demonstrated, antiviral activities against PEDV have not been reported.


id: cord-274765-3wzht843
author: Kweon, Chang-Hee
title: Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate
date: 1999-06-04
extension: .txt
mime: text/plain
words: 2526
sentence: 109
flesch: 51
cache: cache/cord-274765-3wzht843.txt
txt: txt/cord-274765-3wzht843.txt
summary:
  An apparently new syndrome of porcine epidemic diarrhea Some characteristics of a new porcine coronavirus and detection of antigen and antibody by ELISA Experimental infection of pigs with a new porcine enteric coronavirus CV 777 Porcine epidemic diarrhea virus as a cause of persistent diarrhea in a herd of breeding and ®nishing pigs Propagation of the virus of porcine epidemic diarrhea in cell culture Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF Rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction Porcine parvovirus: properties and prevalence of a strain isolated in the US Procedures for identi®cation of arthropod borne viruses A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of eect of HC virus on newcastle disease virus in swine tissue culture. key: cord-274765-3wzht843 authors: Kweon, Chang-Hee; Kwon, Byung-Joon; Lee, Jae-Gil; Kwon, Geon-Oh; Kang, Yung-Bai title: Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate date: 1999-06-04 journal: Vaccine DOI: 10.1016/s0264-410x(99)00059-6 sha: doc_id: 274765 cord_uid: 3wzht843 The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells.


id: cord-272728-inndwa61
author: Jung, Kwonil
date: 2018-08-31
extension: .txt
mime: text/plain
words: 7070
sentence: 221
flesch: 40
cache: cache/cord-272728-inndwa61.txt
txt: txt/cord-272728-inndwa61.txt
title:
  Immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (Gn) pigs infected with porcine epidemic diarrhea virus (PEDV) and serum cytokine responses of Gn pigs to acute PEDV infection

summary:
  We also aimed to detail the pathogenesis of acute PEDV infection, including intestinal distribution of PEDV antigen and serum innate and pro-inflammatory cytokine profiles in infected Gn pigs to understand the relationship of acute PEDV infection with the frequency of serotoninpositive EC cells in the small and large intestines. In our study, the detailed pathogenesis of PEDV in infected Gn pigs was examined to understand the relationship of acute PEDV infection and frequency of serotonin-positive EC cells in the small and large intestines.


id: cord-261043-f9w310tp
author: Ajayi, Toluwalope
title: Forecasting herd-level porcine epidemic diarrhea (PED) frequency trends in Ontario (Canada)
date: 2019-03-01
extension: .txt
mime: text/plain
words: 6535
sentence: 207
flesch: 38
cache: cache/cord-261043-f9w310tp.txt
txt: txt/cord-261043-f9w310tp.txt
summary:
  For random forest classification, prevalence-related variables, whether current or lagged, were the driving force for the 4-week incidence trend (Fig. 2) , however, the current week's mean low temperature (i.e. ont_meanlowtemp) was also highly ranked. The test and training sets were then fed to random forest, neural nets, and classification tree models, with explanatory variables and tuning parameters set to the values used previously (i.e. for the corresponding models with a 70-30 split of the data).


id: cord-278641-8lh5y7j0
author: Chen, Jianing
title: Identification and characterization of PEDV infection in rat crypt epithelial cells
date: 2020-09-17
extension: .txt
mime: text/plain
words: 2781
sentence: 126
flesch: 56
cache: cache/cord-278641-8lh5y7j0.txt
txt: txt/cord-278641-8lh5y7j0.txt
summary:
  The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs.


id: cord-299988-jaekryq5
author: Karte, Claudia
date: 2020-09-10
extension: .txt
mime: text/plain
words: 3147
sentence: 163
flesch: 51
cache: cache/cord-299988-jaekryq5.txt
txt: txt/cord-299988-jaekryq5.txt
title:
  Re-emergence of porcine epidemic diarrhea virus in a piglet-producing farm in northwestern Germany in 2019

summary:
  The prevalence of intestinal trichomonads in Chinese pigs Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Comparison of porcine epidemic diarrhea viruses from Germany and the United States Isolation and characterisation of porcine epidemic diarrhoea virus in Hungary -short communication Complete genome sequence of a porcine epidemic diarrhea s gene indel strain isolated in france in Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in belgium Porcine epidemic diarrhea virus (PEDV) introduction into a naive Dutch pig population in 2014 Outbreak of Porcine Epidemic Diarrhea Virus in Portugal First detection, clinical presentation and phylogenetic characterization of porcine epidemic diarrhea virus in Austria Porcine epidemic Diarrhoea virus in Italy: disease spread and the role of transportation Porcine epidemic diarrhea in Europe: in-detail analyses of disease dynamics and molecular epidemiology Genomnachweis des Porzinen Epidemischen Diarrhoe Virus (PEDV) mittels Real-time RT-PCR. key: cord-299988-jaekryq5 authors: Karte, Claudia; Platje, Nadine; Bullermann, Johannes; Beer, Martin; Höper, Dirk; Blome, Sandra title: Re-emergence of porcine epidemic diarrhea virus in a piglet-producing farm in northwestern Germany in 2019 date: 2020-09-10 journal: BMC Vet Res DOI: 10.1186/s12917-020-02548-4 sha: doc_id: 299988 cord_uid: jaekryq5 BACKGROUND:


id: cord-296791-h8ftslps
author: Junwei, Ge
title: Cloning and sequence analysis of the N gene of porcine epidemic diarrhea virus LJB/03
date: 2006-10-01
extension: .txt
mime: text/plain
words: 2346
sentence: 92
flesch: 54
cache: cache/cord-296791-h8ftslps.txt
txt: txt/cord-296791-h8ftslps.txt
summary:
  A phylogenetic tree was prepared to further examine relationships between PEDV and other coronaviruses based on a comparison of N protein amino acid sequences (Fig. 3) . The N protein is highly immunogenic, more then, the cellular immune response against N protein of some animal coronaviruses can enhance the recovery from the virus infection


id: cord-268010-1m5h3krw
author: Jung, Kwonil
date: 2016-12-02
extension: .txt
mime: text/plain
words: 7592
sentence: 235
flesch: 44
cache: cache/cord-268010-1m5h3krw.txt
txt: txt/cord-268010-1m5h3krw.txt
title:
  Porcine deltacoronavirus infection: Etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis

summary:
  Of the 14 PDCoV-positive samples, 7 (50%) were also positive for PEDV; and 2 were co-infected with PEDV, TGEV, and PDCoV. In infected nursing pigs, similar to PEDV and TGEV infections Saif et al., 2012) , gross lesions are limited to the gastrointestinal tract and are characterized by thin and transparent intestinal walls (proximal jejunum to colon) with accumulation of large amounts of yellow fluid in the intestinal lumen (Chen et al., 2015b; Hu et al., 2016; Jung et al., 2015c; Ma et al., 2015) . In infected nursing pigs, similar to PEDV and TGEV infections Saif et al., 2012) , histological lesions are characterized by acute, multifocal to diffuse, mild to severe atrophic enteritis in the proximal jejunum to ileum (Chen et al., 2015b; Hu et al., 2016; Jung et al., 2015c; Ma et al., 2015; Wang et al., 2015a) , occasionally accompanied by mild vacuolation of superficial epithelial cells in the cecum and colon that coincided with a few PDCoV antigen-positive cecal and colonic epithelial cells (Jung et al., 2015c) .


id: cord-302286-wu6csxve
author: Song, D. S.
title: Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain
date: 2007-02-28
extension: .txt
mime: text/plain
words: 4311
sentence: 189
flesch: 52
cache: cache/cord-302286-wu6csxve.txt
txt: txt/cord-302286-wu6csxve.txt
summary:
  The propagation of a porcine epidemic diarrhea virus in swine cell lines Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR Isolation and serial propagation of porcine epidemic diarrhea virus infection in cell cultures and partial characterization of the isolate Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Mechanism in the pathogenesis of diarrhea: a review Protection against rotavirus induced gastroenteritis in a murine model by passively acquired gastrointestinal but not circulating antibodies Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection Porcine epidemic diarrhea Enteric coronaviruses of animals Isolation of porcine immunoglobulins and determination of the immunoglobulin classes of transmissible gastroenteritis viral antibodies Transmissible gastroenteritis and porcine respiratory coronavirus Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Fecal shedding of a highly cell adapted porcine epidemic diarrhea virus after oral inoculation in pigs Use of an internal control in a quantitative RT-PCR assay for quantitation of porcine epidemic diarrhea virus shedding in pigs Development of mucosal and systemic lymphoproliferative response and protective immunity to human group Induction of antigen specific systemic and mucosal immune responses by feeding animals transgenic plants expressing antigen Development of an ELISA for the detection of antibody isotypes against porcine epidemic diarrhea virus (PEDV) in sow's milk Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhea virus Experimental infection of pigs with a new porcine enteric coronavirus CV777 In vivo morphogenesis of a new porcine enteric coronavirus, CV777 Efficacy of an orally administrated modified-live porcine origin rotavirus vaccine against postweaning diarrhea in pigs Propagation of the virus of porcine epidemic diarrhea in cell culture


id: cord-003503-t6cnjwpd
author: Sung, Ming-Hua
title: Phylogeographic investigation of 2014 porcine epidemic diarrhea virus (PEDV) transmission in Taiwan
date: 2019-03-06
extension: .txt
mime: text/plain
words: 3363
sentence: 143
flesch: 41
cache: cache/cord-003503-t6cnjwpd.txt
txt: txt/cord-003503-t6cnjwpd.txt
summary:
  PloS one Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Detection and molecular diversity of spike gene of porcine epidemic diarrhea virus in China The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing epitopes Cellular entry of the porcine epidemic diarrhea virus Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Coronaviruses: structure and genome expression Role of transportation in spread of porcine epidemic diarrhea virus infection, United States.


id: cord-292690-1p1gnpgf
author: Zang, Yue
date: 2020-08-16
extension: .txt
mime: text/plain
words: 5054
sentence: 161
flesch: 41
cache: cache/cord-292690-1p1gnpgf.txt
txt: txt/cord-292690-1p1gnpgf.txt
title:
  Recombinant Lactobacillus acidophilus expressing S(1) and S(2) domains of porcine epidemic diarrhea virus could improve the humoral and mucosal immune levels in mice and sows inoculated orally

summary:
  Moreover, PEDV specific SIgA levels of oral L. acidophilus vaccine groups were significantly higher than the commercial inactivated vaccine group after 14 dpi, and the L. acidophilus-S1 vaccine efficiently induced mucosal immunity in mice compared to the L. acidophilus-S2 group (Figure 2) . Moreover, the IgG antibody levels of the commercial inactivated vaccine group were obviously higher (p < 0.01) than those of oral vaccine groups between 14 dpi to 42 dpi, and the IgG antibody level of the L. acidophilus-S1 group were higher (p < 0.05) than that of the L. acidophilus-S2 group.


id: cord-260835-ck9z5xsd
author: Kamau, Anthony Ndirangu
date: 2017-01-02
extension: .txt
mime: text/plain
words: 4538
sentence: 170
flesch: 49
cache: cache/cord-260835-ck9z5xsd.txt
txt: txt/cord-260835-ck9z5xsd.txt
title:
  Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus

summary:
  Also, PEDV harvested from pAPN or domain VII expressing NIH3T3 cells was induced indirect plaques in Vero cells confirming successful entry and replication. Cells were blocked with 4% skim milk for 30 min at room temperature and detection of viral antigens was performed using mouse anti-PEDV polysera for 1 h at 37 • C. After 3 times wash with 200 l of 1 X PBS, cells were incubated with horseradish peroxidase (HRP) -conjugated anti-mouse IgG (Santa-Cruz) for 1 h, 37 • C.


id: cord-272973-kzaowysv
author: Joshi, Lok R.
date: 2018-05-03
extension: .txt
mime: text/plain
words: 4285
sentence: 176
flesch: 50
cache: cache/cord-272973-kzaowysv.txt
txt: txt/cord-272973-kzaowysv.txt
title:
  Passive immunity to porcine epidemic diarrhea virus following immunization of pregnant gilts with a recombinant orf virus vector expressing the spike protein

summary:
  (E) Correlation of group mean IgG antibodies in piglet serum with piglet serum NA levels. (F) Correlation of group mean IgA antibodies in piglet serum with piglet serum NA levels.


id: cord-292101-lnap47kp
author: Jung, Kwonil
date: 2015-06-12
extension: .txt
mime: text/plain
words: 3776
sentence: 133
flesch: 38
cache: cache/cord-292101-lnap47kp.txt
txt: txt/cord-292101-lnap47kp.txt
title:
  Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus

summary:
  In inoculated nursing pigs, histologic lesions were limited to the small intestine, mainly jejunum and ileum, and included acute diffuse, severe atrophic enteritis. PEDV antigen-positive cells per jejunal villus of inoculated nursing pigs were 9.3 (AE7.3) at PID 1, 13.9 (AE5.9) at PID 3, and 9.2 (AE4.3) at PID 5.


id: cord-252121-s1zxu5vo
author: Lowe, James
title: Role of Transportation in Spread of Porcine Epidemic Diarrhea Virus Infection, United States
date: 2014-05-17
extension: .txt
mime: text/plain
words: 1501
sentence: 53
flesch: 44
cache: cache/cord-252121-s1zxu5vo.txt
txt: txt/cord-252121-s1zxu5vo.txt
summary:
  The objective of this study was to assess the risks that harvest facilities and transport vehicles engendered in promoting the initial outbreak of a novel disease organism by estimating the incidence of trailer contamination with PEDV during the unloading process at harvest facilities. Harvest facilities serve as a source of contact between many swine farms with different health statuses.


id: cord-289026-v09m2fzw
author: Sun, Yan-gang
date: 2018-10-01
extension: .txt
mime: text/plain
words: 5243
sentence: 235
flesch: 49
cache: cache/cord-289026-v09m2fzw.txt
txt: txt/cord-289026-v09m2fzw.txt
title:
  Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus

summary:
  Lane 1: PEDV S1 protein; Lane 2: pAPN ectodomain and PEDV S1 protein mixtures (1:1); Lane 3: pAPN ectodomain; Lane 4: pAPN ectodomain and PEDV S1t protein mixtures (1:5); Lane 5: PEDV S1t protein. Therefore, the recombinant proteins were heavily glycosylated compared to their calculated molecular masses (pAPN ectodomain: 104 kDa, PEDV S1 protein: 85.5 kDa, PEDV S1t protein: 15.1 kDa).


id: cord-272729-nbgdmavr
author: Kim, Youngnam
title: Ribavirin efficiently suppresses porcine nidovirus replication
date: 2012-10-27
extension: .txt
mime: text/plain
words: 6673
sentence: 226
flesch: 37
cache: cache/cord-272729-nbgdmavr.txt
txt: txt/cord-272729-nbgdmavr.txt
summary:
  Our results demonstrated that ribavirin treatment dose-dependently inhibited the replication of both nidoviruses. N protein staining revealed that the number of cells expressing viral antigen, as quantified by N protein staining results, was also reduced during ribavirin treatment, resulting in a maximum of ∼80% inhibition in response to 50 M and 200 M for PRRSV and PEDV, respectively ( Fig.


id: cord-311561-eiys4mbf
author: Song, Deping
date: 2014-12-04
extension: .txt
mime: text/plain
words: 801
sentence: 22
flesch: 43
cache: cache/cord-311561-eiys4mbf.txt
txt: txt/cord-311561-eiys4mbf.txt
title:
  Full-Length Genome Sequence of a Variant Porcine Epidemic Diarrhea Virus Strain, CH/GDZQ/2014, Responsible for a Severe Outbreak of Diarrhea in Piglets in Guangdong, China, 2014

summary:
  A new coronavirus-like particle associated with diarrhea in swine Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Complete genome sequence of porcine epidemic diarrhea virus strain AJ1102 isolated from a suckling piglet with acute diarrhea in China Molecular epidemiology of porcine epidemic diarrhea virus in China Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain New variants of porcine epidemic diarrhea virus, China Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Complete genome sequence of a highly prevalent isolate of porcine epidemic diarrhea virus in South China Complete genome sequence of a very virulent porcine epidemic diarrhea virus strain, CH/ GDGZ/2012, isolated in Southern China Complete genome sequence of a novel porcine epidemic diarrhea virus in South China Complete genome sequence of novel porcine epidemic diarrhea virus strain GD-1 in China Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Whole-genome analysis of porcine epidemic diarrhea virus (PEDV) from eastern China Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States New variant of porcine epidemic diarrhea virus Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99 key: cord-311561-eiys4mbf authors: Song, Deping; Chen, Yanjun; Peng, Qi; Huang, Dongyan; Zhang, Tiansheng; Huang, Tao; Zhang, Fanfan; Zhou, Xinrong; Tang, Yuxin title: Full-Length Genome Sequence of a Variant Porcine Epidemic Diarrhea Virus Strain, CH/GDZQ/2014, Responsible for a Severe Outbreak of Diarrhea in Piglets in Guangdong, China, 2014 date: 2014-12-04 journal: Genome Announc DOI: 10.1128/genomea.01239-14 sha: doc_id: 311561 cord_uid: eiys4mbf The full-length genome sequence of a variant porcine epidemic diarrhea virus (PEDV) strain, CH/GDZQ/2014, was determined.


id: cord-287947-7kjxbd6t
author: Gao, Ruyi
date: 2020-04-23
extension: .txt
mime: text/plain
words: 5992
sentence: 289
flesch: 51
cache: cache/cord-287947-7kjxbd6t.txt
txt: txt/cord-287947-7kjxbd6t.txt
title:
  Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1/TLR4-MAPK p38 Pathway

summary:
  These results implied that although PEDV infection had no effect on TLR4, TLR4 regulated PEDV infection through activation of signaling cascades. Infection and inflammation of influenza A virus are inhibited on the basis of TLR4-MAPK p38 pathways [41] .


id: cord-295534-bwa4wz94
author: Jung, Kwonil
date: 2015-02-26
extension: .txt
mime: text/plain
words: 7095
sentence: 221
flesch: 37
cache: cache/cord-295534-bwa4wz94.txt
txt: txt/cord-295534-bwa4wz94.txt
title:
  Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis

summary:
  The pathogenicity of epidemic PEDV strains is commonly severe, as evidenced by the high mortality of infected nursing piglets. All strains of epidemic PEDV in Europe, Asia and the US are highly enteropathogenic, as evidenced by the high mortality of infected nursing piglets.


id: cord-305156-w6iqeayr
author: Gallien, Sarah
date: 2018-10-11
extension: .txt
mime: text/plain
words: 4425
sentence: 160
flesch: 53
cache: cache/cord-305156-w6iqeayr.txt
txt: txt/cord-305156-w6iqeayr.txt
title:
  Limited shedding of an S-InDel strain of porcine epidemic diarrhea virus (PEDV) in semen and questions regarding the infectivity of the detected virus

summary:
  In addition, PEDV RNA in Peyer’s patches and in mesenteric lymph nodes was also present for the two inoculated boars. However, to the best of our knowledge, the presence of PEDV RNA in semen of boars infected by an S-InDel PEDV strain has not been studied so far.


id: cord-259296-qsaewje2
author: Wang, Pengcheng
title: Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease
date: 2020-11-11
extension: .txt
mime: text/plain
words: 8207
sentence: 407
flesch: 51
cache: cache/cord-259296-qsaewje2.txt
txt: txt/cord-259296-qsaewje2.txt
summary:
  D Docked conformations of tomatidine with PEDV 3CLpro or inactive 3CLpro. To verify the binding of 3CLpro and tomatidine, PEDV 3CLpro fused with His tag was expressed in soluble form using an E. coli expression system ( Figure 5A(i) ).


id: cord-305859-vt8vwo3y
author: Jung, Kwonil
date: 2017-04-03
extension: .txt
mime: text/plain
words: 3251
sentence: 141
flesch: 52
cache: cache/cord-305859-vt8vwo3y.txt
txt: txt/cord-305859-vt8vwo3y.txt
title:
  Calves are susceptible to infection with the newly emerged porcine deltacoronavirus, but not with the swine enteric alphacoronavirus, porcine epidemic diarrhea virus

summary:
  Our study did not identify which type of cells in the intestines of inoculated calves could be the target for PDCoV replication, resulting in fecal shedding. On the other hand, none of the Gn calves inoculated with PEDV shed detectable PEDV RNA in the feces at PIDs 1-9 (calves #6 and #7) and PIDs 1-17 (calf #5).


id: cord-288253-wqrhiq08
author: Park, Jung-Eun
date: 2015-02-02
extension: .txt
mime: text/plain
words: 5336
sentence: 194
flesch: 42
cache: cache/cord-288253-wqrhiq08.txt
txt: txt/cord-288253-wqrhiq08.txt
title:
  Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus

summary:
  We thus generated porcine APN transgenic mice expressing porcine APN in the brush borders of their small intestines and various tissues (lungs and kidneys). PEDV replication in the small intestines of porcine APN transgenic mice Both wild type and porcine APN transgenic mice were infected with PEDV orally on day 0.


id: cord-302890-eenijt7f
author: Shi, Da
date: 2019-11-02
extension: .txt
mime: text/plain
words: 4899
sentence: 227
flesch: 53
cache: cache/cord-302890-eenijt7f.txt
txt: txt/cord-302890-eenijt7f.txt
title:
  Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector

summary:
  All shRNA tests led to the silencing of N gene expression and suppressed proliferation of PEDV G1 and G2 strains in IEC cells. One day before transfection, IEC cells were seeded into 12-well plates at 5 × 10 4 cells per well without antibiotics and grown at 37 • C overnight with 5% CO 2 .


id: cord-315885-iu5wg5ik
author: Hoang, Hai
date: 2013-12-19
extension: .txt
mime: text/plain
words: 1150
sentence: 30
flesch: 29
cache: cache/cord-315885-iu5wg5ik.txt
txt: txt/cord-315885-iu5wg5ik.txt
title:
  Full-Length Genome Sequence of a Plaque-Cloned Virulent Porcine Epidemic Diarrhea Virus Isolate (USA/Iowa/18984/2013) from a Midwestern U.S. Swine Herd

summary:
  Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus Genome organization of porcine epidemic diarrhoea virus Sequence analysis of the nucleocapsid protein gene of porcine epidemic diarrhoea virus Letter to the editor A new coronavirus-like particle associated with diarrhea in swine Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calicilike virus in porcine postweaning diarrhoea in Hungary Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Prevalence of porcine epidemic diarrhoea virus and transmissible gastroenteritis virus infection in Korean pigs Chinese-like strain of porcine epidemic diarrhea virus Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Propagation of the virus of porcine epidemic diarrhea in cell culture Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Complete genome sequence of porcine epidemic diarrhea virus strain AJ1102 isolated from a suckling piglet with acute diarrhea in China Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain Complete genome sequence of a porcine epidemic diarrhea virus variant Complete genome sequence of a novel porcine epidemic diarrhea virus in south China Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China Complete genome sequence of a recombinant porcine epidemic diarrhea virus strain from eastern China Complete genome sequence of a highly prevalent isolate of porcine epidemic diarrhea virus in south China Complete genome sequence of a variant porcine epidemic diarrhea virus strain isolated in central China Complete genome sequence of novel porcine epidemic diarrhea virus strain GD-1 in China Complete genome sequence of a Vero cell-adapted isolate of porcine epidemic diarrhea virus in eastern key: cord-315885-iu5wg5ik authors: Hoang, Hai; Killian, Mary L.; Madson, Darin M.; Arruda, Paulo H. E.; Sun, Dong; Schwartz, Kent J.; Yoon, Kyoungjin J. title: Full-Length Genome Sequence of a Plaque-Cloned Virulent Porcine Epidemic Diarrhea Virus Isolate (USA/Iowa/18984/2013) from a Midwestern U.S. Swine Herd date: 2013-12-19 journal: Genome Announc DOI: 10.1128/genomea.01049-13 sha: doc_id: 315885 cord_uid: iu5wg5ik Porcine epidemic diarrhea (PED) was recognized in U.S. swine for the first time in early 2013.


id: cord-294559-u0r7oh9z
author: Bian, Hongfen
date: 2019-01-18
extension: .txt
mime: text/plain
words: 5659
sentence: 290
flesch: 60
cache: cache/cord-294559-u0r7oh9z.txt
txt: txt/cord-294559-u0r7oh9z.txt
title:
  A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay

summary:
  It is a classical method for PEDV detection, but it is time-consuming for it needs at least one week. ELISA is a cheap and simple approach for PEDV detection, however, it involves long incubation period, multiple washing steps, and the assistance of a microplate reader.


id: cord-305315-0qt7eth0
author: Cao, Liyan
date: 2015-08-18
extension: .txt
mime: text/plain
words: 4258
sentence: 169
flesch: 43
cache: cache/cord-305315-0qt7eth0.txt
txt: txt/cord-305315-0qt7eth0.txt
title:
  Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-β production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway

summary:
  As the key IFN-β transcription factors, we found that dsRNA-induced activation of IFN regulatory factor 3 (IRF-3) was inhibited after PEDV infection, but not nuclear factor-kappaB (NF-κB). In the upstream of IRF-3, TANK-binding kinase 1 (TBK1)-or inhibitor of κB kinase-ε (IKKε)-mediated IFN-β production was not blocked by PEDV, while RIG-I-and its adapter molecule IFN-β promoter stimulator 1 (IPS-1)-mediated IFN-β production were completely inhibited after PEDV infection.


id: cord-301175-6alsigxk
author: Okda, Faten
date: 2015-08-01
extension: .txt
mime: text/plain
words: 8291
sentence: 335
flesch: 42
cache: cache/cord-301175-6alsigxk.txt
txt: txt/cord-301175-6alsigxk.txt
title:
  Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus

summary:
  Well-validated iELISA, bELISA and FMIA assays for the detection of PEDV antibodies were developed and showed good correlation with IFA and each other. Currently, serum virus neutralization (SVN) tests are the most widely employed serological assays used to detect PEDV antibodies.


id: cord-308170-uqezwbzn
author: Dee, Scott
date: 2014-09-25
extension: .txt
mime: text/plain
words: 3169
sentence: 133
flesch: 55
cache: cache/cord-308170-uqezwbzn.txt
txt: txt/cord-308170-uqezwbzn.txt
title:
  An evaluation of a liquid antimicrobial (Sal CURB®) for reducing the risk of porcine epidemic diarrhea virus infection of naïve pigs during consumption of contaminated feed

summary:
  On 9 designated days during the study period, (days 0, 1, 3, 5, 7, 9, 11, 13 and 15), feed samples were collected from the 3 respective groups. For collecting feed samples, a model of a grain probe was constructed [12] .


id: cord-293512-rcwwx7qw
author: Steinbach, Falko
date: 2016-10-27
extension: .txt
mime: text/plain
words: 1991
sentence: 76
flesch: 48
cache: cache/cord-293512-rcwwx7qw.txt
txt: txt/cord-293512-rcwwx7qw.txt
title:
  A retrospective study detects a novel variant of porcine epidemic diarrhea virus in England in archived material from the year 2000

summary:
  This finding shows the presence of a diverse field PEDV strain in England at the turn of the century, the pathogenicity of which requires further investigation that will only be possible upon rescuing the strain via reverse genetics. Upon recognition of the significant differences to other PEDV strains, next generation full genome sequencing was carried out to avoid the necessity of designing novel primers in multiple rounds of Sanger sequencing for such a large RNA genome.


id: cord-261372-xjbs09gi
author: Sozzi, Enrica
date: 2010-02-28
extension: .txt
mime: text/plain
words: 2093
sentence: 66
flesch: 42
cache: cache/cord-261372-xjbs09gi.txt
txt: txt/cord-261372-xjbs09gi.txt
title:
  Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus

summary:
  Comparison of direct electron microscopy and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea Evaluation of a blocking ELISA using monoclonal antibodies for the detection of porcine epidemic diarrhoea virus and its antibodies Preparation of monoclonal antibodies, strategies and procedure Propagation of the virus of porcine epidemic diarrhoea in cell culture Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by RT-PCR Isolation of porcine epidemic diarrhea virus (PEDV) infection in Korea The correlation between the two methods was higher when testing faecal samples (K =0.97, 95% CI: 0.94–1.00) than testing intestinal samples (K =0.62, 95% CI: 0.35–0.89).


id: cord-267446-rpv19oy6
author: Park, Jung-Eun
date: 2011-06-12
extension: .txt
mime: text/plain
words: 4088
sentence: 140
flesch: 39
cache: cache/cord-267446-rpv19oy6.txt
txt: txt/cord-267446-rpv19oy6.txt
title:
  Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion

summary:
  key: cord-267446-rpv19oy6 authors: Park, Jung-Eun; Cruz, Deu John M.; Shin, Hyun-Jin title: Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion date: 2011-06-12 journal: Arch Virol DOI: 10.1007/s00705-011-1044-6 sha: doc_id: 267446 cord_uid: rpv19oy6 Porcine epidemic diarrhea virus (PEDV) infection in Vero cells is facilitated by trypsin through an undefined mechanism. The present study describes the mode of action of trypsin in enhancing PEDV infection in Vero cells during different stage of the virus life cycle.


id: cord-320559-up1q3k6q
author: Dortmans, J.C.F.M.
title: Porcine epidemic diarrhea virus (PEDV) introduction into a naive Dutch pig population in 2014
date: 2018-05-24
extension: .txt
mime: text/plain
words: 4221
sentence: 158
flesch: 51
cache: cache/cord-320559-up1q3k6q.txt
txt: txt/cord-320559-up1q3k6q.txt
summary:
  We declare no conflict of interest. Surveillance and control of PED coronavirus in pigs in Italy Porcine epidemic diarrhea virus shedding and antibody response in swine farms: a longitudinal study Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Viral disease affects U.S. Pigs: porcine epidemic diarrhea found in at least 11 states Updated epidemiological data on PED Good practices for biosecurity in the pig sector -issues and options in developing and transition countries Coronaviruses: an overview of their replication and pathogenesis Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA Reactivity of porcine epidemic diarrhea virus structural proteins to antibodies against porcine enteric coronaviruses: diagnostic implications Previous infection of sows with a mild strain of porcine epidemic diarrhea virus confers protection against infection with a severe Complete genome sequence of a porcine epidemic diarrhea S gene indel strain isolated in France Comparison of porcine epidemic diarrhea viruses from Germany and the United States Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Multiplex realtime RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus Wild boars harboring porcine epidemic diarrhea virus (PEDV) may play an important role as a PEDV reservoir Manipulation of the porcine epidemic diarrhea virus genome using targeted RNA recombination Cellular entry of the porcine epidemic diarrhea virus Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains Role of transportation in spread of porcine epidemic diarrhea virus infection Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy Outbreak of porcine epidemic diarrhea virus in Portugal Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Comparison of serum neutralization and enzyme-linked immunosorbent assay on sera from porcine epidemic diarrhea virus vaccinated pigs A new coronavirus-like particle associated with diarrhea in swine Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Emergence of porcine epidemic diarrhea virus in southern Germany First detection, clinical presentation and phylogenetic characterization of porcine epidemic diarrhea virus in Austria Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences MEGA6: molecular evolutionary genetics analysis version 6.0 WIN EPISCOPE 2.0: improved epidemiological software for veterinary medicine Distinct characteristics and complex evolution of PEDV strains New variant of porcine epidemic diarrhea virus Pig farming


id: cord-306517-tls0849i
author: Leidenberger, S.
date: 2017-09-07
extension: .txt
mime: text/plain
words: 5935
sentence: 255
flesch: 51
cache: cache/cord-306517-tls0849i.txt
txt: txt/cord-306517-tls0849i.txt
title:
  Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies

summary:
  The Journal of veterinary medical science/the An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Porcine epidemic diarrhea: A retrospect from Europe and matters of debate Porcine epidemic diarrhea: a review of current epidemiology and available vaccines Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy New variants of porcine epidemic diarrhea virus, China Porcine epidemic diarrhea virus variants with high pathogenicity Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Comparison of porcine epidemic diarrhea viruses from Germany and the United States Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Porcine epidemic diarrhea (PED) -Neuausbrüche in deutschen Mastschweinebeständen Porcine Epidemic Diarrhea Virus among Farmed Pigs Complete genome sequence of a porcine epidemic diarrhea s gene indel strain isolated in france in First detection, clinical presentation and phylogenetic characterization of Porcine epidemic diarrhea virus in Austria Outbreak of Porcine Epidemic Diarrhea Virus in Portugal Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in belgium Emergence of porcine epidemic diarrhea virus in southern Germany Porcine Epidemic Diarrhea Virus and Discovery of a Recombinant Swine Enteric Coronavirus Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus New variant of porcine epidemic diarrhea virus Scientific Opinion on porcine epidemic diarrhoea and emerging porcine deltacoronavirus Porcine Epidemic Diarrhea in Europe: In-Detail Analyses of Disease Dynamics and Molecular Epidemiology The ontogeny of the porcine immune system A study of cells in the mammary secretions of sows Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus Immune response of sows vaccinated with attenuated transmissible gastroenteritis virus (TGEV) and recombinant TGEV spike protein vaccines and protection of their suckling pigs against virulent TGEV challenge exposure Evaluation of the effects of PEDv vaccine on PEDv naïve and previously PEDv-exposed sows in a challenge model comparing immune response and preweaning mortality Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions Porcine Epidemic Diarrhea Virus Shedding and Antibody Response in Swine Farms: A Longitudinal Study Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus Experimental Infection of Young Pigs with an Early European Strain of Porcine Epidemic Diarrhoea Virus and a Recent US Strain This research was supported by Boehringer Ingelheim.


id: cord-310579-tnxokfwu
author: Kim, Sung-Jae
date: 2020-07-23
extension: .txt
mime: text/plain
words: 4900
sentence: 210
flesch: 49
cache: cache/cord-310579-tnxokfwu.txt
txt: txt/cord-310579-tnxokfwu.txt
title:
  Molecular Characterization of Porcine Epidemic Diarrhea Virus and Its New Genetic Classification Based on the Nucleocapsid Gene

summary:
  Virus estimated to have killed 7 million-plus pigs Distinct Characteristics and Complex Evolution of PEDV Strains Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains Emerging and re-emerging coronaviruses in pigs Porcine epidemic diarrhoea virus: A comprehensive review of molecular epidemiology, diagnosis, and vaccines Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis The molecular biology of coronaviruses Coronavirus immunogens Identification of the membrane protein of porcine epidemic diarrhea virus Cellular entry of the porcine epidemic diarrhea virus Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody Molecular characterization of a Korean porcine epidemic diarrhea virus strain NB1 Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013-2016 and PEDVs collected from recurrent outbreaks Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Antagonizes Beta Interferon Production by Sequestering the Interaction between IRF3 and TBK1 Molecular Characterizations of Subcellular Localization Signals in the Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression Detection and Phylogenetic Analysis of Porcine Deltacoronavirus in Korean Swine Farms Multiplex Reverse Transcription-PCR for Rapid Differential Detection of Porcine Epidemic Diarrhea Virus, Transmissible Gastroenteritis Virus, and Porcine Group a Rotavirus Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China RDP4: Detection and analysis of recombination patterns in virus genomes 5: An advanced software platform for Bayesian evolutionary analysis Creating the CIPRES Science Gateway for inference of large phylogenetic trees Bayesian phylogenetic site model averaging and model comparison Relaxed Phylogenetics and Dating with Confidence Bayesian random local clocks, or one rate to rule them all Bayesian Coalescent Inference of Past Population Dynamics from Molecular Sequences Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7 Improving the Accuracy of Demographic and Molecular Clock Model Comparison Based on branching patterns of PEDV strains, three main N gene-base genogroups (N1, N2, and N3) and two sub-genogroups (N3a, N3b) were proposed in this study.


id: cord-298401-4szmu1dh
author: Lyoo, Kwang-Soo
date: 2017-12-02
extension: .txt
mime: text/plain
words: 3508
sentence: 134
flesch: 46
cache: cache/cord-298401-4szmu1dh.txt
txt: txt/cord-298401-4szmu1dh.txt
title:
  Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus

summary:
  Based on extensive phylogenetic analysis using obtained sequences, PEDV strains are classified into two major categories: the classical PEDV strain showing lower pathogenicity and highly virulent PEDV strain that emerged in 2010. Moreover, the emergence of PEDV has been reported in western, central and eastern Europe, and the genome sequences were found to be closely related to PEDV strains from the USA, showing a sequence identity of more than 99 per cent.


id: cord-302083-9q1i20o6
author: Jung, Kwonil
date: 2020-06-02
extension: .txt
mime: text/plain
words: 9129
sentence: 231
flesch: 35
cache: cache/cord-302083-9q1i20o6.txt
txt: txt/cord-302083-9q1i20o6.txt
title:
  Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control

summary:
  In infected conventional weaned to feeder pigs (21-56-day-old), a few (<5%) to all of the inoculated pigs began to show diarrhea at PID 1-4, which coincided with or was detected 1-2 days later than the first detection of viral RNA in feces (Gallien et al., 2018a; Gerber et al., 2016; Jung et al., 2015a; Lohse et al., 2017; Madson et al., 2014; Niederwerder et al., 2016; Thomas et al., 2015a) (Table 1) . PEDV antigens were also frequently detected in the intestinal crypt cells or antigen presenting cells, such as macrophages, in the lamina propria or Peyer's patches (Debouck et al., 1981; Jung et al., 2014; Lin et al., 2015a; Madson et al., 2016; Stevenson et al., 2013; Sueyoshi et al., 1995) .


id: cord-308344-ao9z00t7
author: Diep, Nguyen Van
date: 2017-01-17
extension: .txt
mime: text/plain
words: 4211
sentence: 152
flesch: 47
cache: cache/cord-308344-ao9z00t7.txt
txt: txt/cord-308344-ao9z00t7.txt
title:
  Novel Porcine Epidemic Diarrhea Virus (PEDV) Variants with Large Deletions in the Spike (S) Gene Coexist with PEDV Strains Possessing an Intact S Gene in Domestic Pigs in Japan: A New Disease Situation

summary:
  (DOCX) Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines An apparently new syndrome of porcine epidemic diarrhoea A new coronavirus-like particle associated with diarrhea in swine Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Porcine epidemic diarrhea virus variants with high pathogenicity New variants of porcine epidemic diarrhea virus, China Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Distinct characteristics and complex evolution of PEDV strains Comparison of porcine epidemic diarrhea viruses from Germany and the United States Complete genome sequence of a porcine epidemic diarrhea s gene indel strain isolated in france in Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in belgium PubMed Central Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between New variant of porcine epidemic diarrhea virus, United States Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Novel porcine epidemic diarrhea virus variant with large genomic deletion New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs Detection and molecular diversity of spike gene of porcine epidemic diarrhea virus in China Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13 Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus Genetic diversity of ORF3 and spike genes of porcine epidemic diarrhea virus in Thailand Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 PEDV ORF3 encodes an ion channel protein and regulates virus production Molecular epidemiology of porcine epidemic diarrhea virus in China Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate An immunohistochemical investigation of porcine epidemic diarrhoea Porcine epidemic diarrhea: its diagnosis and control Molecular characterization of pig epidemic diarrhoea viruses isolated in Japan from Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 Coronavirus spike proteins in viral entry and pathogenesis Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein The GPRLQPY motif located at the carboxy-terminal of the spike protein induces antibodies that neutralize Porcine epidemic diarrhea virus Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from Defective viral particles and viral disease processes Defective interfering viruses and their potential as antiviral agents Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains Cellular entry of the porcine epidemic diarrhea virus Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses Manipulation of the porcine epidemic diarrhea virus genome using targeted RNA recombination Genetic evolution and tropism of transmissible gastroenteritis coronaviruses Diseases of Swine, Tenth Ed The work was conducted with funding from the University of Miyazaki (grant number 2014-70).


id: cord-331509-p19dg1jw
author: Bigault, Lionel
date: 2020-05-31
extension: .txt
mime: text/plain
words: 4322
sentence: 158
flesch: 50
cache: cache/cord-331509-p19dg1jw.txt
txt: txt/cord-331509-p19dg1jw.txt
title:
  Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR

summary:
  In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the French norm NF U47-600, for the detection and quantification of PEDV viral RNA. This method is the first, validated according a norm for PEDV and may serve as a global reference method to harmonize detection and quantification of PEDV viral RNA in both field and experimental settings.


id: cord-330825-apfcql4m
author: Paraguison-Alili, Rubigilda
title: Phylogenetic tracking of current porcine epidemic diarrhea virus (PEDV) strains in the Philippines
date: 2016-06-28
extension: .txt
mime: text/plain
words: 2179
sentence: 85
flesch: 47
cache: cache/cord-330825-apfcql4m.txt
txt: txt/cord-330825-apfcql4m.txt
summary:
  Since the receptor-binding sites and majority of the neutralization epitopes are located in the S1 portion, this region has been subjected to sequencing and molecular analysis to determine the genetic relatedness of different PEDV viruses This is the first report of PEDV S1 gene sequences from the provincial PEDV hotspots of the Philippines, which include Batangas, Pampanga, Tarlac and Agusan.


id: cord-301355-9lswjro2
author: Fan, Jing-Hui
date: 2015-12-01
extension: .txt
mime: text/plain
words: 3975
sentence: 155
flesch: 45
cache: cache/cord-301355-9lswjro2.txt
txt: txt/cord-301355-9lswjro2.txt
title:
  Development of an enzyme-linked immunosorbent assay for the monitoring and surveillance of antibodies to porcine epidemic diarrhea virus based on a recombinant membrane protein

summary:
  The M protein of PEDV protrudes from the viral envelope, and is considered a superior diagnostic antigen compared with other PEDV proteins (Shenyang et al., 2007) . The nucleotide sequence of the PEDV M gene exhibits low homology (around 50%) with the M gene of other coronaviruses; however, its sequence is highly conserved among different PEDV strains Arndt et al., 2010) .


id: cord-280183-fxhkfjl6
author: Have, P.
date: 1992-04-30
extension: .txt
mime: text/plain
words: 2688
sentence: 113
flesch: 51
cache: cache/cord-280183-fxhkfjl6.txt
txt: txt/cord-280183-fxhkfjl6.txt
title:
  Coronavirus infection in mink (Mustela vision). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus

summary:
  fxhkfjl6 Abstract Antibodies to a transmissible gastroenteritis virus (TGEV)-related coronavirus have been demonstrated in mink sera by indirect immunofluorescence, peroxidase-linked antibody assays and immunoblotting. Four groups of mink sera were selected for evaluation of MCV antibodies: A, 71 mink sera were obtained during 1990 from the Danish Fur Breeders Association as part of routine sampling for plasmacytosis testing (Aleutian disease, ADV).


id: cord-319712-3dikelw6
author: Pujols, Joan
date: 2014-12-05
extension: .txt
mime: text/plain
words: 4735
sentence: 177
flesch: 54
cache: cache/cord-319712-3dikelw6.txt
txt: txt/cord-319712-3dikelw6.txt
title:
  Survivability of porcine epidemic diarrhea virus (PEDV) in bovine plasma submitted to spray drying processing and held at different time by temperature storage conditions

summary:
  Moreover, if SDP would become contaminated Use of spray-dried animal plasma in diets for weanling pigs Experimental infection of pigs with a new porcine enteric coronavirus CV777 An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naive pigs following consumption via natural feeding behavior: proof of concept The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV) Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate New variants of porcine epidemic diarrhea virus, China Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection Infection with porcine epidemic diarrhoea virus Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to naive pigs Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada Porcine epidemic diarrhea A new coronavirus-like particle associated with diarrhea in swine Efficacy of spray-drying to reduce infectivity of pseudo rabies and porcine reproductive and respiratory syndrome (PRRS) viruses and seroconversion in pigs fed diets containing spray-dried animal plasma Neutralizing antibodies against porcine circovirus type 2 in liquid pooled plasma contribute to the biosafety of commercially manufactured spray-dried porcine plasma Inactivation of swine vesicular disease virus in porcine plasma by spray-drying Lack of transmission of porcine circovirus type 2 to weanling pigs by feeding them spray dried porcine plasma Commercial spray-dried porcine plasma does not transmit porcine circovirus type 2 in weaned pigs challenged with porcine reproductive and respiratory syndrome virus Commercially produced spray dried porcine plasma contains high levels of porcine circovirus type 2 (PCV2) DNA but did not transmit PCV2 when fed to naïve pigs Porcine epidemic diarrhea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Evaluation of time and temperature sufficient to inactivate porcine epidemic diarrhea virus in swine feces on metal surfaces Spray dried animal plasma as an alternative to antibiotics in weanling pigs -a review Growth performance of weanling pigs fed spray-dried animal plasma: a review Funding for this study was provided by APC Europe, S.A., Granollers, Barcelona, Spain. Sixty grams of SDBP contained in a large Petri dish were inoculated with 9.0 mL of PEDV cell culture by spraying the liquid virus culture onto the SDBP using a spray bottle.


id: cord-314751-i9rxesrg
author: Oh, Jongsuk
date: 2014-07-10
extension: .txt
mime: text/plain
words: 6085
sentence: 209
flesch: 40
cache: cache/cord-314751-i9rxesrg.txt
txt: txt/cord-314751-i9rxesrg.txt
title:
  Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein

summary:
  However, immunizations involving at least one dose of PEDV S1 protein vaccine (groups 2 and 3) were more efficacious than immunization of sows with two doses of PEDV live and killed vaccines (group 1) in reducing the overall degree of diarrhea, in terms of the duration and severity, in the suckling piglets. In contrast, sows immunized with two doses of PEDV live and S1 protein vaccines (group 2) or three doses of S1 protein vaccine (group 3) at 2-week intervals produced relatively lower neutralizing antibody titers of 1:16 to 1:32 compared to those in group 1.


id: cord-298922-k568hlf4
author: Sun, Dongbo
date: 2015-06-15
extension: .txt
mime: text/plain
words: 5207
sentence: 163
flesch: 37
cache: cache/cord-298922-k568hlf4.txt
txt: txt/cord-298922-k568hlf4.txt
title:
  Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

summary:
  In our study, the integrin recognized sequences of PEDV S protein was analyzed based on Ruoslahti's (1996) report. The CV777 strain of PEDV, kindly provided by Maurice Pensaert at Ghent University (Merelbeke, Belgium), was used in all of our experiments after being adapted to Vero E6 cells, as previously described (Hofmann and Wyler, 1988) .


id: cord-313126-7hrjzapj
author: Chen, Fangzhou
date: 2019-03-08
extension: .txt
mime: text/plain
words: 4470
sentence: 142
flesch: 34
cache: cache/cord-313126-7hrjzapj.txt
txt: txt/cord-313126-7hrjzapj.txt
title:
  Decline of transmissible gastroenteritis virus and its complex evolutionary relationship with porcine respiratory coronavirus in the United States

summary:
  Compared to the S gene of TGEV strains, the S gene of PRCV has 621-681 nt deletions at N-terminal, including in strains from Asia 26 . The traditional TGEV group consisted of TGEV strains isolated from the US, China, Japan, South Korea, England and Mexico.


id: cord-302323-vvo8a4hp
author: Wang, Xiaobo
date: 2015-11-26
extension: .txt
mime: text/plain
words: 5009
sentence: 178
flesch: 47
cache: cache/cord-302323-vvo8a4hp.txt
txt: txt/cord-302323-vvo8a4hp.txt
title:
  Immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes G1 and G2

summary:
  The ELISA results indicated high cross-reactivity between the PAbs and the two types of PEDV S protein. The IFA results indicated that PEDV S protein was present in the cytoplasm of Vero E6 cells (Fig. 5A ).


id: cord-276542-lxwls664
author: Pan, Zhongzhou
date: 2020-06-03
extension: .txt
mime: text/plain
words: 4721
sentence: 195
flesch: 49
cache: cache/cord-276542-lxwls664.txt
txt: txt/cord-276542-lxwls664.txt
title:
  Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

summary:
  Improvements in detection method can bring many benefits. Pathogen monitoring nowadays largely relies on laboratory detection methods.


id: cord-306502-jkqg1qal
author: Dee, Scott
date: 2014-08-05
extension: .txt
mime: text/plain
words: 3754
sentence: 147
flesch: 50
cache: cache/cord-306502-jkqg1qal.txt
txt: txt/cord-306502-jkqg1qal.txt
title:
  An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naïve pigs following consumption via natural feeding behavior: proof of concept

summary:
  However, upon inspection of the bin lumen it was observed that clusters of feed material (feed particles and feed dust) were adhered to the interior walls. For the preparation of challenge for the Treatment group, 30 grams of feed material from the PCR-positive bin samples from Farms A, B and C was pooled and diluted in 30 mL of sterile phosphate buffered saline.


id: cord-273610-cfoq3r3i
author: Tian, Peng-Fei
title: Evidence of Recombinant Strains of Porcine Epidemic Diarrhea Virus, United States, 2013
date: 2014-10-17
extension: .txt
mime: text/plain
words: 1646
sentence: 52
flesch: 43
cache: cache/cord-273610-cfoq3r3i.txt
txt: txt/cord-273610-cfoq3r3i.txt
summary:
  Similar to most of the sequences recently documented in PEDV strains in China and the United States, the S genes of the 24 samples have a 4,161-nt sequence that, compared with the prototype CV777 strain, shows 97.9%-100% sequence identities, and contain 2 notable insertions at amino acids (aa) 56-59 (IGEN) and 139 (N) and a deletion of 2 aa (GK) at aa positions 160 and 161 at the N terminus (6) . Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines A new coronavirus-like particle associated with diarrhea in swine Isolation and characterization of a variant porcine epidemic diarrhea virus in China Chinese-like strain of porcine epidemic diarrhea virus Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Outbreak of porcine epidemic diarrhea in suckling piglets Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission Multiplex reverse transcription-PCR for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and


id: cord-288058-oilurica
author: Cui, Tingting
date: 2020-04-05
extension: .txt
mime: text/plain
words: 7089
sentence: 286
flesch: 49
cache: cache/cord-288058-oilurica.txt
txt: txt/cord-288058-oilurica.txt
title:
  Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes

summary:
  PEDV and TGEV Miller showed a higher infectivity in APN positive cells than in APN negative cells. For CV777 Vero adapted strain, 0.040 ± 0.002% of APN positive cells and 0.020 ± 0.006% of APN negative cells were infected.


id: cord-307408-6wfx0wey
author: Li, Renfeng
date: 2013-11-30
extension: .txt
mime: text/plain
words: 3230
sentence: 118
flesch: 51
cache: cache/cord-307408-6wfx0wey.txt
txt: txt/cord-307408-6wfx0wey.txt
title:
  Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in central China based on the ORF3 gene and the main neutralization epitopes

summary:
  In this study, 14 partial S genes from field PEDV strains were amplified by RT-PCR, cloned, sequenced, and analyzed to study their antigenic variation. Sequence comparison with other PEDV reference strains selected from the GenBank database indicated that the ORF3 genes from the sample strains had a high degree of homology to most Chinese strains.


id: cord-313691-6wq64h2b
author: Zhang, Yuhan
date: 2020-09-17
extension: .txt
mime: text/plain
words: 6577
sentence: 249
flesch: 49
cache: cache/cord-313691-6wq64h2b.txt
txt: txt/cord-313691-6wq64h2b.txt
title:
  Evaluation of Cross-Protection between G1a- and G2a-Genotype Porcine Epidemic Diarrhea Viruses in Suckling Piglets

summary:
  The results showed that CH/JX/01, a highly virulent and dominant G2a PEDV strain currently circulating in China had more severe pathogenicity in vitro and in vivo, and induced more strong immune responses, including higher titers of sIgA in maternal milk than that induced by CV777 PEDV, a prototype of G1a PEDV strain. In our study, biological characteristics between CH/JX/01 and CV777 suggested that CH/JX/01 grew faster and induced more severe CPE in cells and caused more severe diarrhea and death in piglets, indicating that the CH/JX/01 were more virulent than that of classic PEDV CV777.


id: cord-322475-i29t7ce8
author: Chen, Xi
date: 2012-07-29
extension: .txt
mime: text/plain
words: 2810
sentence: 110
flesch: 49
cache: cache/cord-322475-i29t7ce8.txt
txt: txt/cord-322475-i29t7ce8.txt
title:
  Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China

summary:
  The underlines indicate amino acid mutations between strains Experimental infection of pigs with a new porcine enteric coronavirus, CV777 Porcine epidemic diarrhea, in Diseases of Swine Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs Letter to the Editor An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV777, and several coronaviruses Chinese-like strain of porcine epidemic diarrhea virus An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Molecular epidemiology of porcine epidemic diarrhea virus in China A new coronavirus-like particle associated with diarrhea in swine Propagation of the virus of porcine epidemic diarrhea in cell culture The genome organization of the Nidovirales: similarities and differences between arteri-, toro-, and coronaviruses The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus Major receptorbinding and neutralization determinants are located within the same domain of the transmissible gastroenteritis virus (coronavirus) spike protein Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Coronaviruses: structure and genome expression The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Porcine epidemic diarrhea Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Complete genome sequences of a Korean virulent porcine epidemic diarrhea virus and its attenuated counterpart Complete genome sequence of transmissible gastroenteritis coronavirus PUR46-MAD clone and evolution of the purdue virus cluster Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13 Acknowledgments This work was supported by Project Management for Agricultural Science and Technology Achievements Transformation Fund (2010GB2C400209), Science and Technology Major Project of Fujian (2010NZ0002-3), and National Spark Program (2011GA720005).


id: cord-321953-yql6gpd3
author: Barrera, Maritza
date: 2017-12-12
extension: .txt
mime: text/plain
words: 3596
sentence: 155
flesch: 47
cache: cache/cord-321953-yql6gpd3.txt
txt: txt/cord-321953-yql6gpd3.txt
title:
  Tracking the Origin and Deciphering the Phylogenetic Relationship of Porcine Epidemic Diarrhea Virus in Ecuador

summary:
  In this study, a rigorous measurement of the global phylogeographic approach for PEDV strains was performed based on complete S gene sequences. The present work is the first study providing evidences that PEDV strains are circulating into Ecuador swine herds and revealing the molecular characteristic of the PEDV isolate in the South American region.


id: cord-293458-jb7u9xn6
author: Gerdts, Volker
title: Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses
date: 2016-12-02
extension: .txt
mime: text/plain
words: 5584
sentence: 219
flesch: 43
cache: cache/cord-293458-jb7u9xn6.txt
txt: txt/cord-293458-jb7u9xn6.txt
summary:
  Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets Colostral antibodymediated and cell-mediated immunity contributes to innate and antigenspecific immunity in piglets Mechanisms of coronavirus cell entry mediated by the viral spike protein Development of a Novel Vaccine for Porcine Epidemic Diarrhea Virus Passive immunity in transmissible gastroenteritis of swine: immunoglobulin characteristics of antibodies in milk after inoculating virus by different routes Immunology of transmissible gastroenteritis Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity New insights into the dual recruitment of IgA+ B cells in the developing mammary gland Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus T cell-mediated immune response to respiratory coronaviruses Strategies for design and application of enteric viral vaccines p161, a murine membrane protein expressed on mast cells and some macrophages, is mouse CD13/aminopeptidase N Protective efficacy of a replicon particle vaccine in both naïve and previously exposed gilts against porcine epidemic diarrhea virus Coronavirus gene 7 counteracts host defenses and modulates virus virulence Porcine epidemic diarrhea virus nucleocapsid protein antagonizes beta interferon production by sequestering the interaction between IRF3 and TBK1 Safety and antibody response of pigs to an experimental Porcine Epidemic Diarrhea Virus (PEDV) vaccine, killed virus Previous infection of sows with a mild strain of porcine epidemic diarrhea virus confers protection against infection with a severe strain Evaluation of a PED vaccine on piglet mortality and sow immunity The phenotype and activation status of T and NK cells in porcine colostrum suggest these are central/effector memory cells Antibodies and their receptors: different potential roles in mucosal defense Surface-displayed porcine epidemic diarrhea viral (PEDV) antigens on lactic acid bacteria Porcine epidemic diarrhea virus uses cell-surface heparan sulfate as an attachment factor Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections Genetic characterization of porcine epidemic diarrhea virus in Korea from IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): historical and current concepts Replication of transmissible gastroenteritis coronavirus (TGEV) in swine alveolar macrophages A common mucosal chemokine (mucosae-associated epithelial chemokine/CCL28) selectively attracts IgA plasmablasts Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus High-level mucosal and systemic immune responses induced by oral administration with Lactobacillusexpressed porcine epidemic diarrhea virus (PEDV) CCL28 controls immunoglobulin (Ig)A plasma cell accumulation in the lactating mammary gland and IgA antibody transfer to the neonate Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients Efficacy and immunogenicity of recombinant swinepox virus expressing the A epitope of the TGEV S protein Immune evasion of porcine enteric coronaviruses and viral modulation of antiviral innate signaling Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1 Construction of a bivalent DNA vaccine co-expressing S genes of transmissible gastroenteritis virus and porcine epidemic diarrhea virus delivered by attenuated Salmonella typhimurium Mucosal and systemic isotypespecific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus


id: cord-309359-85xiqz2w
author: Song, Daesub
title: Porcine epidemic diarrhea: a review of current epidemiology and available vaccines
date: 2015-07-29
extension: .txt
mime: text/plain
words: 5443
sentence: 224
flesch: 47
cache: cache/cord-309359-85xiqz2w.txt
txt: txt/cord-309359-85xiqz2w.txt
summary:
  USlike strain of por cine epidemic diarrhea virus outbreaks in Taiwan Origin, evolu tion, and genotyping of emergent porcine epidemic diar rhea virus strains in the United States PED vaccine gains conditional approval Sci entific opinion on porcine epidemic diarrhoea and emerg ing porcine deltacoronavirus Genetic variabil ity and phylogeny of current Chinese porcine epidemic diarrhea virus strains based on spike, ORF3, and mem brane genes Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunopro phylaxis The coro navirus spike protein is a class I virus fusion protein: struc tural and functional characterization of the fusion core complex The GPRLQPY motif located at the carboxyterminal of the spike protein induces antibo dies that neutralize porcine epidemic diarrhea virus Identification of the epit ope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus Major recep torbinding and neutralization determinants are located within the same domain of the transmissible gastroenteri tis virus (coronavirus) spike protein Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diar rhea viruses isolated in Korea Cloning and further sequence analysis of the ORF3 gene of wild and attenuatedtype porcine epidemic diarrhea viruses Molecular characteriza tion and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Molecular epidemiology of porcine epidemic diarrhea virus in China Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids express ing spike genes from porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus Complete ge nome sequence of a highly prevalent isolate of porcine epidemic diarrhea virus in South China New variants of porcine epidemic di arrhea virus, China Molecular epidemiology and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea Anti genic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus Four major antigenic sites of the coronavirus transmissible gas troenteritis virus are located on the aminoterminal half of spike glycoprotein S Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during Novel porcine epidemic di arrhea virus variant with large genomic deletion Cell culture isolation and sequence analysis of genetically diverse US porcine epi demic diarrhea virus strains including a novel strain with a large deletion in the spike gene en Republica Dominicana Situacion de la diarrea epidemica porcina en Colombia Direct and rapid detection of porcine epidemic diarrhea virus by RT PCR Rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction PEDV leader sequence and junc tion sites Identification and characteriza tion of new and unknown coronaviruses using RTPCR and degenerate primers Differential detection of trans missible gastroenteritis virus and porcine epidemic diar rhea virus by duplex RTPCR Fecal shedding of a highly cellcultureadapted porcine epidemic diarrhea virus af ter oral inoculation in pigs Multiplex reverse transcrip tionPCR for rapid differential detection of porcine epide mic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus Onestep multiplex RT PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual prim ing oligonucleotide (DPO) system Development of a porcine epidemic diarrhea virus M proteinbased ELISA for virus detection Development of reverse transcription loopme diated isothermal amplification for rapid detection of por cine epidemic diarrhea virus Enteric viral infections of pigs and strategies for in duction of mucosal immunity Protection against rotavirusinduced gastroenteritis in a murine model by passively acquired gastrointestinal but not circulating antibodies Antibody respons es in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus Isolation of porcine immuno globulins and determination of the immunoglobulin class es of transmissible gastroenteritis viral antibodies


id: cord-309693-f2htekhz
author: Yu, Meiling
date: 2017-09-25
extension: .txt
mime: text/plain
words: 6011
sentence: 182
flesch: 38
cache: cache/cord-309693-f2htekhz.txt
txt: txt/cord-309693-f2htekhz.txt
title:
  Immunogenicity of eGFP-Marked Recombinant Lactobacillus casei against Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus

summary:
  The proliferation of spleen lymphocytes upon stimulation with purified COE or 6D proteins was analyzed by an MTT assay. The The proliferation of spleen lymphocytes upon stimulation with purified COE or 6D proteins was analyzed by an MTT assay.


id: cord-302503-7s9f8wje
author: Fu, Yuguang
title: Rapid and efficient detection methods of pathogenic swine enteric coronaviruses
date: 2020-05-19
extension: .txt
mime: text/plain
words: 6360
sentence: 160
flesch: 33
cache: cache/cord-302503-7s9f8wje.txt
txt: txt/cord-302503-7s9f8wje.txt
summary:
  Multiplex nested RT-PCR for the detection of porcine enteric viruses SARS-CoV and emergent coronaviruses: viral determinants of interspecies transmission Field validation of a commercial blocking ELISA to differentiate antibody to transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus and to identify TGEV-infected swine herds Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification Interspecies transmission and emergence of novel viruses: lessons from bats and birds Complete genome sequence of a variant porcine epidemic diarrhea virus strain isolated in China Full-length genome characterization of Chinese porcine deltacoronavirus strain CH/ SXD1/2015 Molecular epidemiology of porcine epidemic diarrhea virus in China Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets Detection and characterization of group C rotaviruses in asymptomatic piglets in Ireland Development of a multiplex RT-PCR for the detection of major diarrhoeal viruses in pig herds in China Transmissible gastroenteritis virus infection induces NF-kappaB activation through RLR-mediated signaling Porcine deltacoronavirus in mainland China A transmissible gastroenteritis in pigs Transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses Transmissible gastroenteritis Epidemiological survey of porcine epidemic diarrhea virus in swine farms in A new bat-HKU2-like coronavirus in swine Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus Establishment and application of a multiplex RT-PCR to differentiate wild-type and vaccine strains of porcine epidemic diarrhea virus Detection, sequence analysis, and antibody prevalence of porcine deltacoronavirus in Taiwan Development of a one-step RT-PCR assay for detection of pancoronaviruses (alpha-, beta-, gamma-, and delta-coronaviruses) using newly designed degenerate primers for porcine and avian`fecal samples A TaqManprobe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses Porcine deltacoronavirus infection: etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR Development of a reverse transcription-nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate Porcine respiratory coronavirus: molecular features and virus-host interactions Detection and characterization of distinct alphacoronaviruses in five different bat species in Denmark Genetic differentiation of the nucleocapsid protein of Korean isolates of porcine epidemic diarrhoea virus by RT-PCR based restriction fragment length polymorphism analysis Complete genome characterization of Korean porcine deltacoronavirus strain KOR/KNU14-04 Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734 New variants of porcine epidemic diarrhea virus, China Detection and differentiation of five diarrhea related pig viruses utilizing a multiplex PCR assay A TaqMan probe-based real-time PCR to differentiate porcine epidemic diarrhea virus virulent strains from attenuated vaccine strains Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains Origin, evolution, and virulence of porcine deltacoronaviruses in the United States Rapid detection, complete genome sequencing, and phylogenetic analysis of porcine deltacoronavirus A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium The first case of porcine epidemic diarrhea in Canada Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis A new coronavirus-like particle associated with diarrhea in swine Complete genome sequence of transmissible gastroenteritis coronavirus PUR46-MAD clone and evolution of the purdue virus cluster Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus Rapid detection of swine transmissible gastroenteritis virus by nested polymerase chain reaction Comparative pathogenesis of enteric viral infections of swine Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages Development and optimization of a sensitive TaqMan(R) real-time PCR with synthetic homologous extrinsic control for quantitation of Human cytomegalovirus viral load Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Newly emerged porcine deltacoronavirus associated with diarrhoea in swine in China: identification, prevalence and full-length genome sequence analysis Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences A novel duplex TaqMan probebased real-time RT-qPCR for detecting and differentiating classical and variant porcine epidemic diarrhea viruses An immunohistochemical investigation of porcine epidemic diarrhoea Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review Outbreak of porcine epidemic diarrhea in suckling piglets An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Development of a TaqMan MGB RT-PCR for the rapid detection of H3 subtype avian influenza virus circulating in China Evidence of recombinant strains of porcine epidemic diarrhea virus Solid phase immune electron microscopy for diagnosis of transmissible gastroenteritis in pigs A real-time TaqMan RT-PCR assay with an internal amplification control for rapid detection of transmissible gastroenteritis virus in swine fecal samples A pancoronavirus RT-PCR assay for detection of all known coronaviruses Reverse transcription cross-priming amplificationnucleic acid test strip for rapid detection of porcine epidemic diarrhea virus Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification Detection and genetic characterization of deltacoronavirus in pigs Development and evaluation of a duplex real-time RT-PCR for detection and differentiation of virulent and variant strains of porcine epidemic diarrhea viruses from the United States Simultaneous detection of five pig viruses associated with enteric disease in pigs using EvaGreen real-time PCR combined with melting curve analysis Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States Coronavirus genomics and bioinformatics analysis Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus Ultrasensitive detection of porcine epidemic diarrhea virus from fecal samples using functionalized nanoparticles Whole-genome analysis of porcine epidemic diarrhea virus (PEDV) from eastern China Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus Porcine deltacoronavirus: overview of infection dynamics, diagnostic methods, prevalence and genetic evolution Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus A multiplex RT-PCR assay for rapid and differential diagnosis of four porcine diarrhea associated viruses in field samples from pig farms in East China from 2010 to 2012 Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus Development of TaqMan real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations In October 2010, a severe PED outbreak caused by a highly virulent PEDV variant emerged in southern China with high mortality ranging from 70 to 100%; the result was devastating damage to the pig farm industry and tremendous economic losses, and later, the PEDV variant spreads to other countries, e.g., USA, Canada, and Mexico ( For the early and rapid detection of PEDV, different types of PCR methods have been developed.


id: cord-317496-6o2upns3
author: Pascual-Iglesias, Alejandro
date: 2019-07-25
extension: .txt
mime: text/plain
words: 7128
sentence: 310
flesch: 43
cache: cache/cord-317496-6o2upns3.txt
txt: txt/cord-317496-6o2upns3.txt
title:
  Recombinant Chimeric Transmissible Gastroenteritis Virus (TGEV)—Porcine Epidemic Diarrhea Virus (PEDV) Virus Provides Protection against Virulent PEDV

summary:
  The ELISA values of the sera were expressed as sample to positive ratio [SP-ratio = (OD of sample − OD of negative control)/(OD of positive control − OD of negative control)]. Heat-inactivated sera were incubated for 1 h at 37 • C in the presence of 100 pfus of PEDV virus in DMEM containing 5% FCS. These viruses required trypsin to efficiently infect Vero cells, and form syncytia, similarly to PEDV virus.


id: cord-255773-b4re5bky
author: Zhang, Qingzhan
date: 2016-01-14
extension: .txt
mime: text/plain
words: 9943
sentence: 377
flesch: 42
cache: cache/cord-255773-b4re5bky.txt
txt: txt/cord-255773-b4re5bky.txt
title:
  Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1

summary:
  We further sought to study whether the CBP degradation by PEDV was mediated by nsp1 protein. Porcine epidemic diarrhea virus nucleocapsid protein antagonizes beta interferon production by sequestering the interaction between IRF3 and TBK1 Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling Mechanisms of activation of interferon regulator factor 3: the role of C-terminal domain phosphorylation in IRF-3 dimerization and DNA binding Genome organization of porcine epidemic diarrhoea virus Rac1 and PAK1 are upstream of IKK-epsilon and TBK-1 in the viral activation of interferon regulatory factor-3 The disruption of ND10 during herpes simplex virus infection correlates with the Vmw110-and proteasome-dependent loss of several PML isoforms Adenovirus small E1A employs the lysine acetylases p300/CBP and tumor suppressor Rb to repress select host genes and promote productive virus infection IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway Porcine reproductive and respiratory syndrome virus induces interleukin-15 through the NF-kappaB signaling pathway Equine arteritis virus does not induce interferon production in equine endothelial cells: identification of nonstructural protein 1 as a main interferon antagonist The viral protein A238L inhibits TNF-alpha expression through a CBP/p300 transcriptional coactivators pathway Degradation of CREB-binding protein and modulation of type I interferon induction by the zinc finger motif of the porcine reproductive and respiratory syndrome virus nsp1alpha subunit Modulation of innate immune signaling by nonstructural protein 1 (nsp1) in the family Arteriviridae Propagation of the virus of porcine epidemic diarrhea in cell culture IRFs: master regulators of signalling by Toll-like receptors and cytosolic pattern-recognition receptors Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to nsp1-induced RNA cleavage Porcine reproductive and respiratory syndrome virus nonstructural protein 4 antagonizes beta interferon expression by targeting the NF-kappaB essential modulator Myocyte enhancer factor (MEF)-2 plays essential roles in T-cell transformation associated with HTLV-1 infection by stabilizing complex between tax and CREB Thogoto virus ML protein suppresses


id: cord-321992-lk2ao6m8
author: Annamalai, Thavamathi
date: 2015-12-15
extension: .txt
mime: text/plain
words: 8590
sentence: 314
flesch: 49
cache: cache/cord-321992-lk2ao6m8.txt
txt: txt/cord-321992-lk2ao6m8.txt
title:
  Age-dependent variation in innate immune responses to porcine epidemic diarrhea virus infection in suckling versus weaned pigs

summary:
  NK cells of infants also had reduced antiviral activity such as defective killing of virus infected cells and reduced cytokine secretion (Jacobson et al., 2013) . Weaned infected piglets had higher NK cell frequencies compared to suckling infected piglets in blood (A) and ileum (B) at PID 5.


id: cord-302819-oj33i2ma
author: Pasick, J
date: 2014-08-07
extension: .txt
mime: text/plain
words: 7964
sentence: 278
flesch: 48
cache: cache/cord-302819-oj33i2ma.txt
txt: txt/cord-302819-oj33i2ma.txt
title:
  Investigation into the Role of Potentially Contaminated Feed as a Source of the First-Detected Outbreaks of Porcine Epidemic Diarrhea in Canada

summary:
  Because the negative control group from this bioassay was co-mingled with the experimental groups, negative control piglets were not available for microscopic examination. Thus, negative control piglets from the second bioassay experiment euthanized at 12 dpi were used for the histopathology and immunohistochemistry observations.


id: cord-279813-mrei5kih
author: Temeeyasen, G.
date: 2017-12-14
extension: .txt
mime: text/plain
words: 6674
sentence: 225
flesch: 39
cache: cache/cord-279813-mrei5kih.txt
txt: txt/cord-279813-mrei5kih.txt
title:
  Differential gene modulation of pattern-recognition receptor TLR and RIG-I-like and downstream mediators on intestinal mucosa of pigs infected with PEDV non S-INDEL and PEDV S-INDEL strains

summary:
  Moreover, PEDV S-INDEL was shown to be clinically relevant in neonates, but clinical disease could not be reproduced in pigs older than three weeks (Annamalai et al., 2015; Chen et al., 2016) . PEDV S-INDEL was shown to be clinically relevant in neonates, but clinical disease could not be reproduced in pigs older than three weeks (Annamalai et al., 2015; Chen et al., 2016) .


id: cord-321739-dnuu6jok
author: Bowman, Andrew S
title: Investigating the introduction of porcine epidemic diarrhea virus into an Ohio swine operation
date: 2015-02-15
extension: .txt
mime: text/plain
words: 4728
sentence: 180
flesch: 50
cache: cache/cord-321739-dnuu6jok.txt
txt: txt/cord-321739-dnuu6jok.txt
summary:
  At the same time, the CN7 east barn housed PEDV negative pigs weighing approximately 50 lbs. In addition, feed pellets collected from unopened bags at the affected sites tested positive for PEDV using RT-PCR.


id: cord-336517-v7z62tld
author: Chu, Hsu-Feng
date: 2018-08-20
extension: .txt
mime: text/plain
words: 5219
sentence: 185
flesch: 48
cache: cache/cord-336517-v7z62tld.txt
txt: txt/cord-336517-v7z62tld.txt
title:
  Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine

summary:
  The coding sequence of the USA strain of PEDV PL2 pro Ubl and catalytic domain (GenBank accession number AHC03501.1; polyprotein residues 1630-1922) was full-gene synthesized (Genomics Co., Taiwan). Like other coronaviral PL pro s, PEDV PL2 pro is not only a deubiquitinating (DUB) protease but also a multifunctional protein which plays a role in regulating host antiviral immune response (Chaudhuri et al., 2011; Clementz et al., 2010; Mielech et al., 2014; Xing et al., 2013; Zheng et al., 2008) .


id: cord-303186-2hxlx1j2
author: Won, Hokeun
date: 2019-07-09
extension: .txt
mime: text/plain
words: 8217
sentence: 291
flesch: 43
cache: cache/cord-303186-2hxlx1j2.txt
txt: txt/cord-303186-2hxlx1j2.txt
title:
  Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus

summary:
  Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus Porcine Viruses: From Pathogenesis to Strategies for Control Fields Virology Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein Porcine epidemic diarrhoea: new insights into an old disease A new coronavirus-like particle associated with diarrhea in swine Deadly pig virus slips through US borders Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Distinct characteristics and complex evolution of PEDV strains US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan Molecular characterization of pig epidemic diarrhoea viruses isolated in Japan from Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): historical and current concepts Does circulating antibody play a role in the protection of piglets against porcine epidemic diarrhea virus? Previous infection of sows with a mild strain of porcine epidemic diarrhea virus confers protection against infection with a severe strain Genetic characterization of porcine epidemic diarrhea virus in Korea from Genetic and phenotypic stability of cold-adapted influenza viruses in a trivalent vaccine administered to children in a day care setting Genotypic stability of cold-adapted influenza virus vaccine in an efficacy clinical trial Development and characterization of cold-adapted viruses for use as live virus vaccines Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Likewise, our study reproduced 100% mortality in 5-day-old conventional piglets inoculated orally with the 10 −2 diluted Aram virus (calculated dose of 10 3 TCID 50 per pig).


id: cord-321814-vt6yio6x
author: Pan, Yongfei
title: Isolation and characterization of a variant porcine epidemic diarrhea virus in China
date: 2012-09-12
extension: .txt
mime: text/plain
words: 3665
sentence: 160
flesch: 48
cache: cache/cord-321814-vt6yio6x.txt
txt: txt/cord-321814-vt6yio6x.txt
summary:
  Pig Farming A new coronavirus-like particle associated with diarrhea in swine Virus-like particles associated with porcine epidemic diarrhoea Current aspects in the etiology of viral diarrheas of swine: occurrence of infections with the epizootic viral diarrhea (EVD) virus Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (EVD): comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Isolation of porcine epidemic diarrhea virus (PEDV) in Korea Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus Chinese-like strain of porcine epidemic diarrhea virus Genetic Characterization of Porcine Epidemic Diarrhea Virus (PEDV) The causative agent of the diarrhea was ultimately identified as porcine epidemic diarrhea virus (PEDV).


id: cord-330035-0d6w8xyd
author: Jeon, Ji Hyun
title: Cellular cholesterol is required for porcine nidovirus infection
date: 2017-09-07
extension: .txt
mime: text/plain
words: 7687
sentence: 274
flesch: 36
cache: cache/cord-330035-0d6w8xyd.txt
txt: txt/cord-330035-0d6w8xyd.txt
summary:
  Taken together, our data indicate that cell membrane cholesterol is required for porcine nidovirus entry into cells, and pharmacological drugs that hamper cholesterol-dependent virus entry may have antiviral potential against porcine nidoviruses. Likewise, the present study indicated that pharmacological depletion of cellular cholesterol had no effect on the levels of pCD163 and pAPN expression in porcine cells.


id: cord-322683-wkrj6n1d
author: Zhang, Pengfei
date: 2020-07-13
extension: .txt
mime: text/plain
words: 4080
sentence: 168
flesch: 47
cache: cache/cord-322683-wkrj6n1d.txt
txt: txt/cord-322683-wkrj6n1d.txt
title:
  Cellular Poly(C) Binding Protein 2 Interacts with porcine epidemic diarrhea virus Papain-Like Protease 1 and Supports Viral Replication

summary:
  A DNA sequence encoding PEDV PLP1 protein was cloned into pCMV-HA as previously described to yield the pCMV-PLP1-HA expression vector (Yu et al., 2019) . PLP1 overexpression enhanced endogenous accumulation of PCBP2 protein in a dose-dependent manner (Fig. 6) .


id: cord-340438-9q3ic0ye
author: Zhang, Jianqiang
date: 2018-02-21
extension: .txt
mime: text/plain
words: 1728
sentence: 76
flesch: 59
cache: cache/cord-340438-9q3ic0ye.txt
txt: txt/cord-340438-9q3ic0ye.txt
title:
  Identification of porcine epidemic diarrhea virus variant with a large spike gene deletion from a clinical swine sample in the United States

summary:
  Subsequent PEDV S gene-based differential rRT-PCR revealed that these samples were negative for S INDEL PEDV (C T > 40) but positive for non-S INDEL PEDV. PEDV S protein has multiple functions including (1) mediating receptor binding through its S1 subunit (aa 1-729) and fusion of the viral and cellular membranes during cell entry through its S2 subunit (aa 730-1386); (2) harboring neutralization epitopes.


id: cord-307110-eiobmxp2
author: Zhao, Shan
title: Serological Screening for Coronavirus Infections in Cats
date: 2019-08-13
extension: .txt
mime: text/plain
words: 6749
sentence: 288
flesch: 52
cache: cache/cord-307110-eiobmxp2.txt
txt: txt/cord-307110-eiobmxp2.txt
summary:
  FCoV S1 domains of both type 1 and 2, namely S1 0-CD , were expressed as murine Fc fusion proteins in HEK-293T cells as described above. S1 S1 0


id: cord-342923-prgorr3d
author: Li, Zhonghua
date: 2018-03-13
extension: .txt
mime: text/plain
words: 4181
sentence: 199
flesch: 51
cache: cache/cord-342923-prgorr3d.txt
txt: txt/cord-342923-prgorr3d.txt
title:
  Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication

summary:
  Austria The coronavirus nucleocapsid is a multifunctional protein Molecular characterizations of subcellular localization signals in the nucleocapsid protein of porcine epidemic diarrhea virus The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage Porcine epidemic diarrhea virus nucleocapsid protein antagonizes β interferon production by sequestering the interaction between IRF3 and TBK1 Cellular hnRNP A2/B1 interacts with the NP of influenza A virus and impacts viral replication The nucleocapsid protein of SARS coronavirus has a high binding affinity to the human cellular heterogeneous nuclear ribonucleoprotein A1 Design Principles of a Dynamic RNP Machine Heterogeneous nuclear ribonucleoprotein A1 in health and neurodegenerative disease: From structural insights to post-transcriptional regulatory roles Isolation of an active gene encoding human hnRNP protein A1: Evidence for alternative splicing The Swiss army knife of gene expression Viral and Cellular mRNA Translation in Coronavirus-Infected Cells Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain He, Q. iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus Comparative Genomic Analysis of Classical and Variant Virulent Parental/Attenuated Strains of Porcine Epidemic Diarrhea Virus The nucleocapsid protein of coronavirus mouse hepatitis virus interacts with the cellular heterogeneous nuclear ribonucleoprotein A1 in vitro and in vivo A1-mediated translational regulation of the G quadruplexcontaining RON receptor tyrosine kinase mRNA linked to tumor progression Cytoplasmic relocalization of heterogeneous nuclear ribonucleoprotein A1 controls translation initiation of specific mRNAs Heterogeneous nuclear ribonucleoprotein A1 regulates RNA synthesis of a cytoplasmic virus Multiple Type A/B Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) Can Replace hnRNP A1 in Mouse Hepatitis Virus RNA Synthesis Blocking eIF4E-eIF4G Interaction as a Strategy To Impair Coronavirus Replication Nuclear Proteins Hijacked by Mammalian Cytoplasmic Plus Strand RNA Viruses As shown in Figure 3 , cellular hnRNP A1 protein was only detected in the presence of flag tagged PEDV N by CO-IP.


id: cord-330475-mameyzih
author: Shi, Da
date: 2014-03-13
extension: .txt
mime: text/plain
words: 5590
sentence: 212
flesch: 37
cache: cache/cord-330475-mameyzih.txt
txt: txt/cord-330475-mameyzih.txt
title:
  Molecular Characterizations of Subcellular Localization Signals in the Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus

summary:
  Furthermore, by utilizing fusion proteins with green fluorescent protein (GFP), deletion mutations or site-directed mutagenesis of PEDV N protein, coupled with live cell imaging and confocal microscopy, it was revealed that, a region spanning amino acids (aa), 71–90 in region 1 of the N protein was sufficient for nucleolar localization and R87 and R89 were critical for its function. To identify whether there were subcellular localization signals in PEDV N protein, we first conducted a bioinformatics analysis on the protein using existing motif prediction algorithms.


id: cord-339924-tsmnkuhw
author: Jung, Kwonil
title: Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs
date: 2014-04-17
extension: .txt
mime: text/plain
words: 1865
sentence: 65
flesch: 41
cache: cache/cord-339924-tsmnkuhw.txt
txt: txt/cord-339924-tsmnkuhw.txt
summary:
  Six 10-to 35-day-old pigs were randomly assigned to a PEDV-infected group (pigs 1-5) or a negative control group (pig 6). Pigs 1-3 and 5 were inoculated orally and/or intranasally with 6.3-9.0 log 10 genomic equivalents (GE) of PEDV strain PC21A; pig 4 was exposed to the virus by indirect contact with inoculated pig 3.


id: cord-347475-ttmactz0
author: Mesquita, J. R.
title: Outbreak of Porcine Epidemic Diarrhea Virus in Portugal, 2015
date: 2015-09-07
extension: .txt
mime: text/plain
words: 1517
sentence: 53
flesch: 52
cache: cache/cord-347475-ttmactz0.txt
txt: txt/cord-347475-ttmactz0.txt
summary:
  Typical clinical symptoms of PED include watery diarrhea, vomiting, dehydration, and Porcine epidemic diarrhea virus (PEDV; family Coronaviridae, subfamily Coronavirinae, genus Alphacoronavirus) is a highly contagious virus responsible for enteric disease in swine characterized by an acute onset of symptoms including severe watery diarrhea, vomiting, dehydration, and high mortality in suckling piglets (ICTV, 2012; Song and Park, 2012) . Comparison of porcine epidemic diarrhea viruses from Germany and the United States Virus Taxonomy: Classification and Nomenclature of Viruses; Ninth Report of the International Committee on Taxonomy of Viruses Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines 2013:


id: cord-354729-dpaz01np
author: Huan, Changchao
date: 2019-08-22
extension: .txt
mime: text/plain
words: 2527
sentence: 101
flesch: 50
cache: cache/cord-354729-dpaz01np.txt
txt: txt/cord-354729-dpaz01np.txt
title:
  Characterization and evolution of the coronavirus porcine epidemic diarrhoea virus HLJBY isolated in China

summary:
  For pathogenicity evaluation of PEDV HLJBY strain, colostrum deprivation piglets were challenged with PEDV HLJBY, and PEDV reference strain CV777 as a control, the results showed that animals challenged with either of these PEDV strains developed diarrhoea, and histopathological examination of small intestines of challenged animals showed acute viral enteritis with villous atrophy in either PEDV HLJBY‐P10 or PEDV CV777‐P8 inoculated piglets. cluding a positive-sense single-strand RNA genome, which can cause a devastating enteric disease characterized with dehydration and watery diarrhoea (Pensaert & de Bouck, 1978) . To reveal the characteristic and the diversity between PEDV strains currently circulating in China and other PEDV strains outside, the complete genomic sequence of PEDV HLJBY strain was determined and analysed, and the pathogenicity of PEDV HLJBY strain in newborn piglets was also evaluated.


id: cord-344309-6c2wttxg
author: Lin, Huixing
date: 2018-08-20
extension: .txt
mime: text/plain
words: 4415
sentence: 186
flesch: 51
cache: cache/cord-344309-6c2wttxg.txt
txt: txt/cord-344309-6c2wttxg.txt
title:
  Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein

summary:
  This established S1 indirect ELISA is capable of detecting serum antibodies against PEDV, and due to its high sensitivity and specificity, it could be applied for serological evaluation and indirect diagnosis of PEDV infection. Serum cross-reactivity of S1 indirect ELISA to other pathogens To validate the cross-reactivity, this S1 indirect ELISA was utilized to test porcine serum positive for other swine pathogens, namely, porcine transmissible gastroenteritis virus (TGEV), swine rotavirus (PoRV), porcine kobuvirus (PKV), porcine bocavirus (PBoV), porcine norovirus (PNoV), porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and enterotoxigenic E. coli (ETEC),


id: cord-346457-2mq2aije
author: Wang, Zhilin
date: 2020-06-22
extension: .txt
mime: text/plain
words: 4779
sentence: 155
flesch: 43
cache: cache/cord-346457-2mq2aije.txt
txt: txt/cord-346457-2mq2aije.txt
title:
  Rapid differentiation of PEDV wild-type strains and classical attenuated vaccine strains by fluorescent probe-based reverse transcription recombinase polymerase amplification assay

summary:
  This method contained two real-time RT-RPAs, a universal real time RT-RPA assay targeting nucleocapsid gene was used to detect all types of PEDV strains and another PEDV vaccine real time RT-RPA could identity PEDV classical attenuated vaccine strains according to ORF1 24-nucleotides deletion region of three classical attenuated vaccine strains (PEDV attenuated vaccine KC189944, attenuated CV777 and DR13) and five Vero cell-adapted isolates (JS2008, SDM, SQ2014, SC1402, HLJBY) genome sequences, which were compared to 38 PEDV wild-type strains published by GenBank (Fig. 1 ). In the study, a differential one-step quantitative real-time fluorescent reverse transcription recombinase polymerase amplification (real-time RT-RPA) method based on the PEDV universal real-time RT-RPA assay was established according to the ORF1 deletion sequences of three classical attenuated vaccine strains (PEDV attenuated vaccine KC189944, attenuated CV777 and DR13) and five Vero cell-adapted isolates (JS2008, SDM, SQ2014, SC1402, HLJBY), which could effectively differentiate PEDV classical attenuated vaccine strains from wild-type strains (PEDV classical wild strains and variant strains).


id: cord-332811-kjgah8ts
author: Lee, Do Hyun
date: 2015-06-23
extension: .txt
mime: text/plain
words: 5958
sentence: 208
flesch: 40
cache: cache/cord-332811-kjgah8ts.txt
txt: txt/cord-332811-kjgah8ts.txt
title:
  Immunoprophylactic effect of chicken egg yolk antibody (IgY) against a recombinant S1 domain of the porcine epidemic diarrhea virus spike protein in piglets

summary:
  The sections were then counterstained with DAPI and examined using a fluorescence microscope at 2009 magnification The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Experimental infection of pigs with a new porcine enteric coronavirus, CV 777 Sequence of the spike protein of the porcine epidemic diarrhoea virus Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA Propagation of the virus of porcine epidemic diarrhea in cell culture Spike glycoprotein cleavage recognition site analysis of infectious bronchitis virus Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Isolation of porcine epidemic diarrhea virus (PEDV) in Korea Immunoprophylactic effect of chicken egg yolk immunoglobulin (IgY) against porcine epidemic diarrhea virus (PEDV) in piglets A method of egg yolk fractionation. Characterization of fractions Mutations within the nuclear localization signal of the porcine reproductive and respiratory syndrome virus nucleocapsid protein attenuate virus replication Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea The N-terminal region of the porcine epidemic diarrhea virus spike protein is important for the receptor binding Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea Reemergence of porcine epidemic diarrhea virus on Jeju Island Full-genome sequence analysis of a variant strain of porcine epidemic diarrhea virus in South Korea Cytokine production in immortalized porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus New variants of porcine epidemic diarrhea virus, China US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review Deadly pig virus slips through US borders Contribution of the porcine aminopeptidase N (CD13) receptor density to porcine epidemic diarrhea virus infection Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein Letter to the editor A new coronavirus-like particle associated with diarrhea in swine Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs Chinese-like strain of porcine epidemic diarrhea virus Porcine reproductive and respiratory syndrome virus nucleocapsid protein modulates interferon-b production by inhibiting IRF3 activation in immortalized porcine alveolar macrophages Human telomerase reverse transcriptase-immortalized porcine monomyeloid cell lines for the production of porcine reproductive and respiratory syndrome virus Diseases of swine Molecular cloning: a laboratory manual Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Proteolytic cleavage of peplomeric glycoprotein E2 of MHV yields two 90K subunits and activates cell fusion Spike protein region (aa 636789) of porcine epidemic diarrhea virus is essential for induction of neutralizing antibodies An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Acknowledgments This research was supported by Technology Development Program for Bio-Industry, Ministry for Agriculture, Food and Rural Affairs, Republic of Korea (311007-05-1-HD120).


id: cord-331542-wy068c6o
author: Kong, Ning
title: Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus
date: 2020-04-03
extension: .txt
mime: text/plain
words: 3705
sentence: 164
flesch: 49
cache: cache/cord-331542-wy068c6o.txt
txt: txt/cord-331542-wy068c6o.txt
summary:
  The indirect ELISA and western blot analysis showed that the SE polyclonal antisera had the highest antibody titers against PEDV S1 protein ( Fig. 1a and b) , suggesting that SE protein was the important immunodominant region of S1. The results showed that MAb 2E10 reacted with fragments spanning aa 690 to 736 (SE2) and 707 to 761 (SE3) but not aa 667 to 711 (SE1) or 742 to 798 (SE4), suggesting that the epitope recognized by 2E10 was located in aa 707 to 736 of PEDV S1 protein (Fig. 3b) .


id: cord-334218-bkjfy66e
author: Lin, Jung-Da
date: 2016-01-15
extension: .txt
mime: text/plain
words: 3642
sentence: 137
flesch: 51
cache: cache/cord-334218-bkjfy66e.txt
txt: txt/cord-334218-bkjfy66e.txt
title:
  Impact of Mated Female Nonproductive Days in Breeding Herd after Porcine Epidemic Diarrhea Virus Outbreak

summary:
  PEDV outbreak were compared using a two-way ANOVA for multiple comparisons. In addition to this pig farm, we also analyzed the productivity index during the 1-year period before and after PEDV outbreaks from one Taiwanese farrow-to-finish herd (500 sows).


id: cord-346872-k5d5793a
author: Yuan, Peng
date: 2018-09-03
extension: .txt
mime: text/plain
words: 5654
sentence: 235
flesch: 46
cache: cache/cord-346872-k5d5793a.txt
txt: txt/cord-346872-k5d5793a.txt
title:
  Three Main Inducers of Alphacoronavirus Infection of Enterocytes: Sialic Acid, Proteases, and Low pH

summary:
  Most α-CoVs use APN as cell entry receptor. TGEV and PEDV S proteins have many similarities in their secondary structure.


id: cord-316908-8ti75mru
author: Wei, Xiaona
date: 2020-02-10
extension: .txt
mime: text/plain
words: 10526
sentence: 439
flesch: 54
cache: cache/cord-316908-8ti75mru.txt
txt: txt/cord-316908-8ti75mru.txt
title:
  PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway

summary:
  Considering that CoVs take advantage of different pathways to enter cells, whether different subtypes of PEDV invade cells by different ways and whether PEDV enter different types of cells through different ways remains to be determined. These differences between GDS01 and GDS09 strains may be due to the difference of S gene especially the S1 region of S gene (homology was about 92%), which is responsible for cell entry and membrane fusion by binding with receptor.


id: cord-331919-6kistim2
author: Song, Daesub
date: 2012-01-22
extension: .txt
mime: text/plain
words: 4270
sentence: 219
flesch: 54
cache: cache/cord-331919-6kistim2.txt
txt: txt/cord-331919-6kistim2.txt
title:
  Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

summary:
  ORF3 genes have been used to divide Chinese field strains and PEDV reference strains into 3 groups; further, Chinese field strains appear to be closely related to Korean strains, but genetically different from PEDV vaccine strains. Immunochromatographic assay kits can be used at farms in order to detect PEDV S proteins with 92% sensitivity and 98% specificity.


id: cord-311255-zaa8i9vh
author: Kim, Youngnam
date: 2014-07-31
extension: .txt
mime: text/plain
words: 8058
sentence: 280
flesch: 34
cache: cache/cord-311255-zaa8i9vh.txt
txt: txt/cord-311255-zaa8i9vh.txt
title:
  Porcine epidemic diarrhea virus induces caspase-independent apoptosis through activation of mitochondrial apoptosis-inducing factor

summary:
  311255 cord_uid: zaa8i9vh Abstract The present study sought to investigate whether porcine epidemic diarrhea virus (PEDV) induces apoptosis and to elucidate the mechanisms associated with apoptotic cell death after PEDV infection. At present, it is unknown whether PEDV triggers apoptotic cell death, and if so, whether virusinduced apoptosis aids or worsens viral replication and pathogenicity.


id: cord-345940-adg264vb
author: Wanitchang, Asawin
date: 2018-08-22
extension: .txt
mime: text/plain
words: 5062
sentence: 180
flesch: 45
cache: cache/cord-345940-adg264vb.txt
txt: txt/cord-345940-adg264vb.txt
title:
  Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus

summary:
  The full-length PEDV S derived from PEDV AVCT12 (Gen-Bank LC053455.1), PEDV YN144 (GenBank KT021232.1) and PEDV G2 (field isolate) were codon-optimized for expression in mammalian cells and synthesized (Genscript and Synbio Tech). Because the construction of recombinant PEDV bearing specific mutations in the S gene can be technically challenging, the available data concerning the binding of PEDV S to sialic-acid-containing glycoproteins in the context of infection have so far been limited to the use of natural isolates such as the PEDV-UU strain [15] .


id: cord-353245-es7b1rs0
author: Song, Deping
date: 2015-03-19
extension: .txt
mime: text/plain
words: 4286
sentence: 133
flesch: 47
cache: cache/cord-353245-es7b1rs0.txt
txt: txt/cord-353245-es7b1rs0.txt
title:
  Molecular Characterization and Phylogenetic Analysis of Porcine Epidemic Diarrhea Viruses Associated with Outbreaks of Severe Diarrhea in Piglets in Jiangxi, China 2013

summary:
  To address the genetic/antigenic variations, and phylogenetic characteristics of PEDV strains associated with 2013 Jiangxi PED outbreaks, two representative PEDV strains, designated CH/JX-1/2013 and CH/JX-2/2013 (the GenBank accession numbers are KF760557 and KJ526096, respectively), were used for sequencing the full-length genome. Notably, an amino acid substitution was found in the middle of one neutralizing epitope Phylogenetic trees based on the complete genome, aa sequences of structural proteins and ORF3 of PEDV strains.


id: cord-339871-jso21mbx
author: Lee, Sunhee
date: 2018-05-16
extension: .txt
mime: text/plain
words: 3099
sentence: 96
flesch: 37
cache: cache/cord-339871-jso21mbx.txt
txt: txt/cord-339871-jso21mbx.txt
title:
  Genomic and antigenic characterization of porcine epidemic diarrhoea virus strains isolated from South Korea, 2017

summary:
  Lee http://orcid.org/0000-0002-5930-5461 Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets Nidovirales: A new order comprising Coronaviridae and Arteriviridae Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA Nidovirales: Evolving the largest RNA virus genome Genetic characterization of porcine epidemic diarrhea virus in Korea from Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence Isolation of porcine epidemic diarrhea virus (PEDV) in Korea Coronaviridae Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): Historical and current concepts Porcine epidemic diarrhea virus: An emerging and reemerging epizootic swine virus Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112 Reemergence of porcine epidemic diarrhea virus on Jeju Island Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea Outbreak-related porcine epidemic diarrhea virus strains similar to US strains Complete genome sequence of a novel S-insertion variant of porcine epidemic diarrhea virus from South Korea Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea Full-genome sequence analysis of a variant strain of porcine epidemic diarrhea virus in South Korea Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein New variants of porcine epidemic diarrhea virus, China US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan Ministry of agriculture, Forestry, and Fisheries Deadly pig virus slips through US borders Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein The first case of porcine epidemic diarrhea in Canada Letter to the editor Porcine epidemic diarrhea (PED) in Europe and strategies to control outbreaks A new coronavirus-like particle associated with diarrhea in swine Chinese-like strain of porcine epidemic diarrhea virus Diseases of Swine The neighbor-joining method: A new method for reconstructing phylogenetic trees Molecular cloning: A laboratory manual Emergence of porcine epidemic diarrhea virus in the United States: Clinical signs, lesions, and viral genomic sequences An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan MEGA4: key: cord-339871-jso21mbx authors: Lee, Sunhee; Lee, Changhee title: Genomic and antigenic characterization of porcine epidemic diarrhoea virus strains isolated from South Korea, 2017 date: 2018-05-16 journal:


id: cord-339012-4juhmjaj
author: Hou, Wei
date: 2020-07-28
extension: .txt
mime: text/plain
words: 6784
sentence: 241
flesch: 39
cache: cache/cord-339012-4juhmjaj.txt
txt: txt/cord-339012-4juhmjaj.txt
title:
  Rapid host response to an infection with Coronavirus. Study of transcriptional responses with Porcine Epidemic Diarrhea Virus

summary:
  Differential expressed genes (DEGs) in PEDV infected cells were compared to DEGs responding in Vero cells infected with Mammalian Orthoreovirus (MRV). A decrease in CT-values for PEDV was not observed before 6 h post inoculation (6 h.p.i), indicating that replication in PEDV infected cells started later than was observed for MRV (at 4 h.p.i).


id: cord-343780-084lq92r
author: Hsu, Tien-Huan
title: Detection, sequence analysis, and antibody prevalence of porcine deltacoronavirus in Taiwan
date: 2018-07-26
extension: .txt
mime: text/plain
words: 2088
sentence: 78
flesch: 53
cache: cache/cord-343780-084lq92r.txt
txt: txt/cord-343780-084lq92r.txt
summary:
  PDCoV-positive samples were further examined by traditional reverse transcription polymerase chain reaction (RT-PCR) with primers PDCoV-N1 (5'-ACC ATC GCT CCA AGT CAT TCTTG-3') and PDCoV-N2 (5'-GAG TGG AGT TGG GTG GGT TTA reverse-transcription reaction was performed at 50 °C for 30 minutes, and then a standard polymerase chain reaction was performed: 30 cycles of 94 °C (30 seconds), 55 °C (30 seconds), and 72 °C (1 minute). The nucleotide sequence data were analyzed using Chromas Lite, and the deduced amino acid sequences of the open reading frames were compared to other PDCoV sequences using BLAST.


id: cord-348669-mizygp4j
author: Beall, Anne
date: 2016-01-05
extension: .txt
mime: text/plain
words: 6058
sentence: 205
flesch: 35
cache: cache/cord-348669-mizygp4j.txt
txt: txt/cord-348669-mizygp4j.txt
title:
  Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A

summary:
  in conventional 9-day-old nursing piglets vs. 26-day-old weaned pigs Swine enteric coronavirus diseases (SECD), including porcine epidemic diarrhea virus (PEDv) Origin, evolution, and virulence of porcine deltacoronaviruses in the United States A novel pathogenic mammalian orthoreovirus from diarrheic pigs and swine blood meal in the United States Distinct characteristics and complex evolution of PEDV strains Understanding human coronavirus HCoV-NL63 Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Outbreak of porcine epidemic diarrhea in suckling piglets Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan Porcine epidemic diarrhea: a review of current epidemiology and available vaccines Receptor usage and cell entry of porcine epidemic diarrhea coronavirus Porcine epidemic diarrhea virus uses cellsurface heparan sulfate as an attachment factor Genome organization of porcine epidemic diarrhoea virus Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence PEDV ORF3 encodes an ion channel protein and regulates virus production Manipulation of the porcine epidemic diarrhea virus genome using targeted RNA recombination Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus Systematic assembly of a full-length infectious clone of human coronavirus NL63 Development of mouse hepatitis virus and The appearance of the porcine respiratory coronavirus has created new problems and perspectives Detection and genetic characterization of deltacoronavirus in pigs Genetic manipulation of porcine epidemic diarrhea virus (PEDV) recovered from a full-length infectious cDNA clone Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain Avian influenza virus transmission to mammals Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology USDA licenses first vaccine for porcine epidemic diarrhea.


id: cord-344558-1jgqofbr
author: Kocherhans, Rolf
title: Completion of the Porcine Epidemic Diarrhoea Coronavirus (PEDV) Genome Sequence
date: 2001
extension: .txt
mime: text/plain
words: 2841
sentence: 147
flesch: 61
cache: cache/cord-344558-1jgqofbr.txt
txt: txt/cord-344558-1jgqofbr.txt
summary:
  Later, a mixture of up to six antigenome sense primers based on PEDV specific sequences or the degenerate primers and random hexamer primer (purchased from Schmidheini AG; Balgach, Switzerland) was used for first strand cDNA synthesis. The multiple sequence alignments revealed several putative functional domains common to coronavirus sequences [23, 26] located on the deduced amino acid sequence of ORF1ab of PEDV.


id: cord-327000-oyg3oyx1
author: Li, Shasha
title: Porcine Epidemic Diarrhea Virus and the Host Innate Immune Response
date: 2020-05-11
extension: .txt
mime: text/plain
words: 11139
sentence: 482
flesch: 41
cache: cache/cord-327000-oyg3oyx1.txt
txt: txt/cord-327000-oyg3oyx1.txt
summary:
  These cap structures enhance the initiation of translation of viral proteins, protect viral mRNAs against cellular 5 -3 -exoribonuclease and limit the recognition of viral RNA by host innate system [120, 121] . Although several studies have been performed to understand the pathogenicity of PEDV, there remains limited information about the interaction between viral proteins and host cell factors during viral infection.


id: cord-355465-qjtifwhd
author: Van Diep, Nguyen
date: 2018-03-14
extension: .txt
mime: text/plain
words: 6024
sentence: 239
flesch: 52
cache: cache/cord-355465-qjtifwhd.txt
txt: txt/cord-355465-qjtifwhd.txt
title:
  Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013–2016 and PEDVs collected from recurrent outbreaks

summary:
  The coronavirus nucleocapsid is a multifunctional protein The identification and characterization of two novel epitopes on the Nucleocapsid protein of the porcine epidemic diarrhea virus An immunohistochemical investigation of porcine epidemic diarrhoea Porcine epidemic diarrhea: its diagnosis and control Molecular characterization of pig epidemic diarrhoea viruses isolated in Japan from Isolation and molecular characterization of porcine epidemic diarrhea viruses collected in Japan in 2014 US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between Novel porcine epidemic diarrhea virus (PEDV) variants with large deletions in the spike (S) gene coexist with PEDV strains possessing an intact S gene in domestic pigs in Japan: a new disease situation MEGA6: molecular evolutionary genetics analysis version 6.0 EMBOSS: the European molecular biology open software suite Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Distinct characteristics and complex evolution of PEDV strains New variant of porcine epidemic diarrhea virus Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus Isolation and experimental inoculation of an S INDEL strain of porcine epidemic diarrhea virus in Japan Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Genetic variation analyses of porcine epidemic diarrhea virus isolated in mid-eastern China from 2011 to 2013 Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in Central China during 2010-2012 outbreaks Phosphorylation events during viral infections provide potential therapeutic targets Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein Specific asparagine-linked glycosylation sites are critical for DC-SIGN-and L-SIGN-mediated severe acute respiratory syndrome coronavirus entry Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during 2011-2013 in Guangdong, China Measure to mitigate loss caused by PED (porcine epidemic diarrhea) using maternal immunity The work was conducted with funding from the University of Miyazaki (grant number 2014-70) and Japan Society for the Promotion of Science (KAKENHI program, grant number 17H04639). Due to the complexity in discriminating, isolating, and individually sequencing PEDV strains, only specimens containing PEDVs with an intact S gene were used in our study.


id: cord-355991-4zu69e0y
author: Piñeyro, Pablo Enrique
date: 2018-09-24
extension: .txt
mime: text/plain
words: 3923
sentence: 186
flesch: 41
cache: cache/cord-355991-4zu69e0y.txt
txt: txt/cord-355991-4zu69e0y.txt
title:
  First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

summary:
  The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation. Historically, TGEV infection has followed a seasonal pattern, becoming more prevalent during winter months perhaps due to increased viral survival in colder temperatures and with less exposure to sunlight.


id: cord-340202-ikptxviu
author: Van Diep, Nguyen
title: US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014
date: 2015-12-02
extension: .txt
mime: text/plain
words: 3791
sentence: 130
flesch: 46
cache: cache/cord-340202-ikptxviu.txt
txt: txt/cord-340202-ikptxviu.txt
summary:
  As a result, the genetic characteristics and origins of prevailing PEDVs in Japan, the efficacy of PEDV vaccines being used in Japan in protecting previously well pigs from prevailing PEDVs, and the genetic differences between vaccine strains and field PEDV isolates remain critically important questions that have yet to be fully elucidated. Pairwise alignment of field Japanese PEDV isolates demonstrated high nucleotide and amino acid sequence identity between strains (99.1-100.0 and 97.2-100 %, respectively).


id: cord-319460-n4ezxnjc
author: Bertasio, Cristina
title: Porcine Epidemic Diarrhea Virus Shedding and Antibody Response in Swine Farms: A Longitudinal Study
date: 2016-12-15
extension: .txt
mime: text/plain
words: 5929
sentence: 211
flesch: 47
cache: cache/cord-319460-n4ezxnjc.txt
txt: txt/cord-319460-n4ezxnjc.txt
summary:
  QPCR and clinical data were useful in understanding the dynamics of PEDV infections and, therefore, in implementing appropriate control measures. None of the farms had reported PEDV infections prior to this study, and thus, we can surmise that the sows had been recently infected at the time of their enrollment in the study, while piglets were infected after birth, through contact with infected sows.


id: cord-354052-x4ckzw64
author: Li, Chunhua
title: Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA Recombination
date: 2013-08-02
extension: .txt
mime: text/plain
words: 4940
sentence: 193
flesch: 46
cache: cache/cord-354052-x4ckzw64.txt
txt: txt/cord-354052-x4ckzw64.txt
summary:
  siRNA knockdown of ORF3 gene in PEDV infected cells reduced the number of particles released from the cells [5] . Virus Genes Outbreak of porcine epidemic diarrhea in suckling piglets Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in korea PEDV leader sequence and junction sites PEDV ORF3 encodes an ion channel protein and regulates virus production Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus Propagation of the virus of porcine epidemic diarrhea in cell culture Role of proteases in the release of porcine epidemic diarrhea virus from infected cells Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain:


id: cord-341469-7guojyay
author: Park, Seong-Jun
date: 2011-01-06
extension: .txt
mime: text/plain
words: 3721
sentence: 126
flesch: 46
cache: cache/cord-341469-7guojyay.txt
txt: txt/cord-341469-7guojyay.txt
title:
  Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea

summary:
  The nucleotide sequences of the full-length M and ORF3 genes of Korean PEDV field isolates were aligned using ClustalX version 1.83 Sequences of complete ORF3 genes of Korean PEDV field isolates were determined and compared to those of other PEDV reference strains.


id: cord-344845-52rehsd5
author: Opriessnig, Tanja
date: 2017-10-26
extension: .txt
mime: text/plain
words: 5163
sentence: 217
flesch: 56
cache: cache/cord-344845-52rehsd5.txt
txt: txt/cord-344845-52rehsd5.txt
title:
  Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b challenge

summary:
  Pigs orally vaccinated with an experimental heterologous G1b live vaccine had a tendency for a shortened viral shedding duration; whereas pigs vaccinated intramuscularly with an experimental heterologous G1b live vaccine were not protected. In this study growing pigs were used to test and compare the efficacy of live or inactivated vaccines to protect pigs against challenge with a highly virulent G2b


id: cord-331652-oc5s1if2
author: Trudeau, Michaela P.
date: 2016-06-24
extension: .txt
mime: text/plain
words: 5999
sentence: 250
flesch: 49
cache: cache/cord-331652-oc5s1if2.txt
txt: txt/cord-331652-oc5s1if2.txt
title:
  Comparison of Thermal and Non-Thermal Processing of Swine Feed and the Use of Selected Feed Additives on Inactivation of Porcine Epidemic Diarrhea Virus (PEDV)

summary:
  Feed samples were spiked with PEDV and then heated to 120–145°C for up to 30 min or irradiated at 0–50 kGy. Another set of feed samples spiked with PEDV and mixed with Ultracid P (Nutriad), Activate DA (Novus International), KEM-GEST (Kemin Agrifood), Acid Booster (Agri-Nutrition), sugar or salt was incubated at room temperature (~25°C) for up to 21 days.


id: cord-316134-lkd2mj27
author: Sungsuwan, Suttipun
date: 2020-01-15
extension: .txt
mime: text/plain
words: 9264
sentence: 307
flesch: 41
cache: cache/cord-316134-lkd2mj27.txt
txt: txt/cord-316134-lkd2mj27.txt
title:
  Nucleocapsid proteins from other swine enteric coronaviruses differentially modulate PEDV replication

summary:
  No detectable fluorescence was observed from co-transfection with the empty pCAGGS vector, confirming the role of PEDV N protein during at least the first round of viral RNA transcription and viral protein translation (Fig. 7A ). Specifically, we asked if N proteins from TGEV or PDCoV can interact with PEDV N and possibly affect PEDV replication.


id: cord-355119-sdg9zdc1
author: Lin, Huixing
date: 2016-04-22
extension: .txt
mime: text/plain
words: 3062
sentence: 120
flesch: 50
cache: cache/cord-355119-sdg9zdc1.txt
txt: txt/cord-355119-sdg9zdc1.txt
title:
  Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge

summary:
  Submit your next manuscript to BioMed Central and we will help you at every step: Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy New variants of porcine epidemic diarrhea virus, China Molecular characterization and phylogenetic analysis of new variants of the porcine epidemic diarrhea virus in Gansu Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Complete Genome Sequence of Porcine Epidemic Diarrhea Virus Strain USA/Colorado/2013 from the United States Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus The GPRLQPY motif located at the carboxyterminal of the spike protein induces antibodies that neutralize Porcine epidemic diarrhea virus Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain Propagation of the virus of porcine epidemic diarrhea in cell culture Detection and genetic diversity of porcine group A rotaviruses in historic (2004) and recent (2011 and 2012) Approximately 80 to 100% of PEDV-infected piglets die within 24 h of being infected with virulent PEDV strains, resulting in tremendous economic losses to the swine industry


id: cord-341521-dntkdwkj
author: Luo, Yi-Ran
date: 2020-03-24
extension: .txt
mime: text/plain
words: 4092
sentence: 131
flesch: 45
cache: cache/cord-341521-dntkdwkj.txt
txt: txt/cord-341521-dntkdwkj.txt
title:
  Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway

summary:
  We demonstrate that the inhibitors of Chk.2 and H2A.X can reduce the G1/G0phase arrest of Vero cells infected by PEDV, indicating that the effect of PEDV infection on Vero cell cycles is diminished with a blockade of the DNA damagesignalling pathway. Respiratory syncytial virus matrix protein induces lung epithelial cell cycle arrest through a p53 dependent pathway Expression of protein N of porcine transmissible gastroenteritis in swine intestinal epithelial cells and effect and influence on cell cycle Murine coronavirus nonstructural protein p28 arrests cell cycle in G0/G1 phase Comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus Transmissible gastroenteritis virus infection induces cell cycle arrest at S and G2/M phases via p53-dependent pathway Preparation and characterization of monoclonal antibodies against the N protein of porcine epidemic diarrhoea virus SHpd/2012 strain Cell cycle perturbations induced by infection with the coronavirus infectious bronchitis virus and their effect on virus replication Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China Synchronization of cell populations in G1/S and G2/M phases of the cell cycle Development and application of a TaqMan Real-Time RT-PCR assay for the detection of a porcine epidemic diarrhea virus strain in China Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan, China Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in central China based on the ORF3 gene and the main neutralization epitopes Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China


id: cord-344421-rmnck42f
author: Theuns, Sebastiaan
date: 2018-06-29
extension: .txt
mime: text/plain
words: 8656
sentence: 369
flesch: 46
cache: cache/cord-344421-rmnck42f.txt
txt: txt/cord-344421-rmnck42f.txt
title:
  Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus

summary:
  Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus date: 2018-06-29 journal: Sci Rep DOI: 10.1038/s41598-018-28180-9 sha: doc_id: 344421 cord_uid: rmnck42f Enteric diseases in swine are often caused by different pathogens and thus metagenomics are a useful tool for diagnostics. metagenomics is a valuable asset for diagnostics in pigs, leading to discovery of novel viruses and identification of porcine viral enteric disease complexes.


id: cord-339546-m7rqr886
author: de Arriba, M.L
date: 2002-01-01
extension: .txt
mime: text/plain
words: 6477
sentence: 216
flesch: 47
cache: cache/cord-339546-m7rqr886.txt
txt: txt/cord-339546-m7rqr886.txt
title:
  Isotype-specific antibody-secreting cells in systemic and mucosal associated lymphoid tissues and antibody responses in serum of conventional pigs inoculated with PEDV

summary:
  IgA and IgG ASC responses were evident in the intestinal lymphoid tissues from PID 21, the highest number of specific ASC corresponded to the duodenum lamina propria. Similar experiences with other porcine enteric viruses such as TGEV or rotavirus (Chen et al., 1995; Van Cott et al., 1993; Yuan et al., 1996) showed consistent IgA and IgG ASC responses as early as PID 12, with the maximum amounts of cells detected also being higher than those described in the report.


id: cord-343132-qqhivgkq
author: Chang-Liao, Wan-Ping
date: 2020-07-15
extension: .txt
mime: text/plain
words: 6012
sentence: 219
flesch: 36
cache: cache/cord-343132-qqhivgkq.txt
txt: txt/cord-343132-qqhivgkq.txt
title:
  Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains

summary:
  The absorbance was measured at 570 nm using a microplate reader (Victor 3 , PerkinElmer Inc., Waltham, MA, USA), and percentages of cell metabolic activity were calculated as follows: However, pretreatment of PEDV cells with the cell-wall fractions of Ln. mesenteroides YPK30 for 24 h before infection with PEDV did not impede PEDV replication.


id: cord-330772-i7cfmw9x
author: Peng, Ju-Yi
date: 2019-12-03
extension: .txt
mime: text/plain
words: 4646
sentence: 173
flesch: 47
cache: cache/cord-330772-i7cfmw9x.txt
txt: txt/cord-330772-i7cfmw9x.txt
title:
  Evaluation of antiviral activity of Bacillus licheniformis-fermented products against porcine epidemic diarrhea virus

summary:
  To evaluate the cytotoxicity of BLFP crude extract in vitro, Vero cells were first grown in a 96-well microplate (Corning Life Sciences, Corning, NY, USA) at a density of 20,000 cells per well 1 day prior to the experiment. Normalized data were plotted against concentrations of BLFP crude extract and fitted to a non-linear regression curve using Graph-Pad Prism (GraphPad Software, San Diego, CA).


id: cord-329227-sqetz7h6
author: Hou, Yixuan
date: 2018-11-07
extension: .txt
mime: text/plain
words: 8065
sentence: 300
flesch: 48
cache: cache/cord-329227-sqetz7h6.txt
txt: txt/cord-329227-sqetz7h6.txt
title:
  Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs

summary:
  Collectively, our data support the hypothesis that both motifs are involved in the regulation of PEDV S proteins on the cell surface: the KVHVQ motif retains the S proteins in the ERGIC, limiting its transport to the cell surface, whereas the Yxx⌽ motif decreases the amount of surface S proteins via endocytosis. The mutants YA, YA-A, MK-P10, and MK-P10-V had the second highest level of cell surface S proteins.


id: cord-349249-jwvz1ux2
author: Singh, Gagandeep
date: 2019-10-22
extension: .txt
mime: text/plain
words: 6560
sentence: 231
flesch: 39
cache: cache/cord-349249-jwvz1ux2.txt
txt: txt/cord-349249-jwvz1ux2.txt
title:
  A Minimally Replicative Vaccine Protects Vaccinated Piglets Against Challenge With the Porcine Epidemic Diarrhea Virus

summary:
  The objective of this study was to evaluate the heat and RNAse treated PEDV vaccine for its safety, immunogenicity and ability to reduce viremia in a weanling piglet model, with the ultimate goal of developing a process which can potentially reduce lead vaccine development time, is safe and be easily applied to newly emerging strains. At DPC 3 and 7, challenge viral RNA was not detected in any of the pigs vaccinated with the heat and RNAse treated PEDV vaccine (Figure 4) , while 4 of the 6 pigs administered the irradiated vaccine were positive by RT-qPCR on DPC3.


