key: cord-262776-6k7tcgfs
authors: Burnouf, Thierry; Griffiths, Elwyn; Padilla, Ana; Seddik, Salwa; Stephano, Marco Antonio; Gutiérrez, José-María
title: Assessment of the viral safety of antivenoms fractionated from equine plasma
date: 2004-09-30
journal: Biologicals
DOI: 10.1016/j.biologicals.2004.07.001
sha: 
doc_id: 262776
cord_uid: 6k7tcgfs

Abstract Antivenoms are preparations of intact or fragmented (F(ab′)2 or Fab) immunoglobulin G (IgG) used in human medicine to treat the severe envenomings resulting from the bites and stings of various animals, such as snakes, spiders, scorpions, or marine animals, or from the contact with poisonous plants. They are obtained by fractionating plasma collected from immunized horses or, less frequently, sheep. Manufacturing processes usually include pepsin digestion at acid pH, papain digestion, ammonium sulphate precipitation, caprylic acid precipitation, heat coagulation and/or chromatography. Most production processes do not have deliberately introduced viral inactivation or removal treatments, but antivenoms have never been found to transmit viruses to humans. Nevertheless, the recent examples of zoonotic diseases highlight the need to perform a careful assessment of the viral safety of antivenoms. This paper reviews the characteristics of equine viruses of antivenoms and discusses the potential of some manufacturing steps to avoid risks of viral contamination. Analysis of production parameters indicate that acid pH treatments and caprylic acid precipitations, which have been validated for the manufacture of some human IgG products, appear to provide the best potential for viral inactivation of antivenoms. As many manufacturers of antivenoms located in developing countries lack the resources to conduct formal viral validation studies, it is hoped that this review will help in the scientific understanding of the viral safety factors of antivenoms, in the controlled implementation of the manufacturing steps with expected impact on viral safety, and in the overall reinforcement of good manufacturing practices of these essential therapeutic products.

Antivenoms are important biopharmaceutical products made from the plasma of immunized horses or sheep. They are used in human medicine to treat the potentially severe pathophysiological complications resulting from the bites and stings from various animals, such as snakes, scorpions, spiders, cnidarians, lepidopterans or fishes, as well as from intoxications with plants [1, 2] . Envenomings are a serious health problem worldwide and are most particularly dreadful in rural areas of the developing world [3] , where shortage of antivenom products [4] and lack of sufficient medical facilities explain numerous fatalities [5, 6] .

Snake bites represent the major cause of envenoming. Case fatality associated with some snake bites reach 50% or more but can be reduced to less than 5% through antivenom therapy, the only available current treatment [5,7e9] . Global mortality from snake bites may range from 50,000 to 100,000 per year, but these figures are likely underestimated [5, 10] . Viperid snake bite envenoming induces local effects, such as swelling, pain, necrosis, hemorrhage and blistering, often accompanied by secondary infection [9] . Systemic viperid envenoming is characterized by a complex pathophysiological profile including coagulopathy, hemorrhage, hypovolaemia, shock and acute renal failure [9, 11] . Progressive paralysis may be caused by elapid snake venom neurotoxins, and by some viperid venoms displaying neurotoxic effects. Some elapid venoms also induce local necrosis and rhabdomyolysis [9] . Snake bite survivors may have major chronic physical and neurological disability.

Scorpion stings are the second major cause of human fatalities from envenoming (probably amounting to several hundreds per year). Scorpion venoms contain toxins which target sodium, potassium, calcium and chloride channels, causing direct effects and release of neutrotransmitters such as acetylcholine and catecholamines, inducing intense local pain and potentially fatal neurotoxic and hemodynamic disturbances [12] . The role of antivenom in the treatment of scorpion stings and other arachnids remains controversial but several reports support their manufacture and use, particularly in severe cases [13, 14] .

Fatalities have also occurred from envenoming by jellyfish, and venomous fishes. Long-term anecdotal experience supports the beneficial effect of stonefish antivenoms [2] , but those may need to be given very early to fight a rapid onset of cardiotoxicity. Stings by cnidarians, lepidoptera, centipedes and coneshells and bites by spiders, ticks and one genus of octopus, probably account for about 100 deaths per year [5] . Finally, envenomings by massive attacks of Africanised bees cause some 30 deaths each year in the Americas [5] .

Antivenoms are most often made by fractionation of plasma of horses (less frequently sheep) that have been immunized with crude venoms [1] . Pooled hyperimmune plasma is processed to purify the horse immunoglobulin G (IgG) fraction that may be subjected to enzymatic treatment to obtain F(ab#) 2 and Fab antibody fragments, and caprylate or ammonium sulphate precipitation to improve their purity [1] . To some extent, some manufacturing steps have features similar to those used to prepare human plasma-derived IgG. Although there is no known transmission of any infectious agent through antivenoms (albeit under circumstances where rigorous clinical patient follow-up is difficult), theoretical concerns about the possibility of transmission of horse/sheep infectious agents to humans do exist. Recent natural transmissions of zoonotic diseases highlight the possible exchanges between humans and the natural reservoirs of biologic agents found in animals [15] , and the inherent risk of emerging diseases [16] . Examples of such infections originating from animal (or avian) pathogens include human immunodeficiency virus, Ebola, Hantaan, Lassa, Nipah viruses and other paramyxoviruses, equine morbilli virus, West Nile virus, and probably severe acute respiratory syndrome (SARS) coronavirus [17, 18] . The parenteral transmission of animal viruses to humans is also possible. Infectious SV40 virus of rhesus monkeys was a contaminant of early polio vaccines which were administered to a large number of people. Whether this was the way in which this virus was introduced into the human population is unclear and a controversial issue.

The risk of contamination of antivenoms by equine virus is, therefore, of theoretical concern and has been debated at a recent World Health Organization (WHO) workshop [5] . Recently, the Committee for Proprietary Medicinal Product (CPMP) of the European Medicine Evaluation Agency has published a Note for Guidance on the manufacture and quality control of animal immunoglobulins and immunsera [19] . However, most manufacturers of antivenoms are located in the developing world and some of them may not be directly exposed to the state-of-the-art process validation concepts, nor have the financial and logistics capability to perform extensive viral validation studies to assess the viral reduction potential of the process they use. Therefore, we found it helpful to examine the viral safety of antivenoms by first reviewing the characteristics of equine viruses. We then evaluated the theoretical ability of manufacturing methods of antivenoms to inactivate or remove those viruses, through a comparison with the well validated technologies used to manufacture human plasma-derived IgG preparations. Finally, we want to emphasize that applying good manufacturing practices in the production of antivenoms, from the control of the animals to that of all production steps, and ensuring traceability in the whole chain of manufacture, represent the current best investment in the quality and safety of these products.

Horses can harbour enveloped and non-enveloped viruses. The main structural characteristics of those viruses are presented in Table 1 ; epidemiological and clinical data and testing methods are described briefly below.

EAV is a 50e60 nm single-stranded (ss)-RNA virus of the Arteriviridae family [20] . EAV causes an equine viral arteritis, an endotheliotropic viral disease [21] . Transmission occurs via respiratory and reproductive routes. There is a variety of clinical signs, and strains vary in virulence [21] , but severe infection can lead to abortions in pregnant mares or neonatal foal death. A one-tube real-time TaqMan RT-PCR assay was developed for detecting EAV. The test was validated using seminal plasma and nasal secretions [22] . A DNA vaccine was shown to induce long-term immunization [23] .

BDV is a 70e130 nm ss-RNA virus belonging to the new Bornaviridae family, order Mononegavirales. Borna disease, known as 'disease of the head', is a sporadically occurring, progressive viral polioencephalomyelitis that primarily affects horses and sheep. After a few weeks to several months incubation, BDV can cause locomotor and sensory dysfunction followed by paralysis and death. BDV exists world-wide in horses, sheep, cattle, cats, dogs and ostriches and can affect a large number of warm-blooded animal species, including humans [24, 25] . The infection can be fatal, but the majority of carriers are asymptomatic [26] . Cross-species transmission of this commensal virus has not been proven, but zoonotic aspects of BDV should be considered [26] . BDV-specific antibodies and viral RNA have been found in humans with various psychiatric disorders. Diagnosis can be made serologically, but also by antigen markers in peripheral white blood cells, combined with nucleic acid amplification [26] .

These are closely related 40e70 nm ss-RNA alphaviruses [arbovirus type A] of the Togaviridae family that are transmitted by arthropods (usually mosquitoes). Symptoms of EEEV infections, an important multisystemic zoonotic disease, include anorexia and colic, changes in sensorium, hyperexcitability, and terminal severe depression. Organ coagulative necrosis and CNS lesions are observed [27] . WEEV is not as neuroinvasive as EEEV and the encephalitis caused is not as severe as that caused by EEEV. A test to detect EEE and WEE viral RNA has recently been developed [28] . VEEV is present in both humans and horses but no evidence of transmission from horses to humans by normal routes of contamination has been found. VEEV has been identified in pharyngeal secretions and is stable when aerosolized; it has been shown to be stable in dried blood and exudates. Vaccines have been developed against those three viruses [29] ; one possible case of viral transmission in horse has been suspected following vaccination [30] .

ECV is a 75e160 nm ss-RNA virus of the Coronaviridae family. It has been isolated from feces of a diarrhoeic foal, and has close antigenic and/or genetic relationships with mammalian group 2 coronaviruses [31] .

EFV, also known as spumavirus, is a 80e100 nm ss-RNA virus of the Retroviridae family. It belongs to the nonpathogenic, complex unconventional retroviruses and has been isolated in nonhuman primates, cattle, cats, and more recently in the blood of horses [32] .

EHVs are large 125e150 nm double-stranded (ds) DNA gamma herpes viruses. EHV1 and EHV4 cause much damage to the horse industry and are ubiquitous in the equine population. They are responsible for life-long latent infections in their hosts even in those with natural or vaccine-induced immunity [33] . EHV1 strains are associated with respiratory disease, abortion, and paresis/paralysis, whereas EHV4 strains induce respiratory disease [34] . EHV2 and EHV5 have a less clear pathogenicity and distribution within the equine population. EHV2 may have an aetiological role in ocular disease [35] . The prevalence of EHV2 in adult horses was found to be up to 68% in Sweden and 71% in the United Kingdom. The prevalence of EHV5 DNA was 15 and 24% in adult horses in Hungary and the United Kingdom, respectively [36] .

EIAV is a 80e100 nm ss-RNA lentivirus of the same Retroviridae family as HIV. Most infected horses are asymptomatic, with life-long latent infection of leucocytes until stressed (e.g. by pregnancy, corticosteroids, surgical operation, disease) or until new virus variants arise. Viraemia then increases by 100-fold. Red blood cells become coated by the virus particles and are then lysed by complement, causing jaundice, oedema, hemorrhagic diarrhoea, petechial hemorrhages. EIAV can be transmitted from horse to horse by blood. Recently a reverse-transcriptase polymerase chain reaction assay (RT-PCR) has been described for quantifying EIAV RNA in equine plasma [37] .

EIV is a 80e120 nm ss-RNA virus of the Orthomyxoviridae. Equine influenza is one of the most economically important contagious respiratory diseases of horses [38] .

HeV is a 150 nm ss-RNA virus [39] . The virus is probably transmitted from bats to horses, and causes natural disease in humans and horses. It is the reference virus for a proposed new genus within the Paramyxoviridae family, which also includes another newly identified zoonotic bat virus (Nipah), and the Salem virus. HeV has been recognized in Australia as a new zoonotic disease of horses since 1994/1995. This lethal zoonotic viral agent is endemic in certain species of fruit bats (flying-foxes) and over 20% of them in eastern Australia are seropositive [40] . Six species of flying-foxes in Papua New Guinea have tested positive for antibodies to HeV [40] . HeV does not appear to transmit readily between horses under natural and experimental conditions, or from horses to humans [41] . Horses can be infected by oronasal routes and can excrete HeV in urine and saliva [41] . The virus appears to be spread by close contact with body fluids, such as froth from infected lungs [42] , via nasal discharge, saliva and/or urine [43] . The most important clinical and pathological manifestation of HeV infection in horses and humans is severe interstitial pneumonia caused by viral infection of small blood vessels. From 1994 to 1999, epizootic and epidemiological episodes of meningoencephalitis and severe acute respiratory syndromes were reported in Australia, Malaysia and Singapore [15, 44] . It may also cause nervous disease. Three incidents of HeV disease in horses have been recorded in Australiadtwo in 1994, which caused the death of two humans and 15 horses and one in 1999, which involved the death of a single horse [45] . Infected horses can develop a severe and often fatal respiratory disease characterized by dyspnoea, vascular endothelial damage and pulmonary oedema. Nervous signs may also occur. A number of diagnostic methods have been developed [46] , based on the examination of blood, lung, kidney, spleen, and, if nervous signs are present, also of the brain. PCR assays have been developed. A rapid and sensitive RT-PCR assay using a fluorogenic (TaqMan) probe was developed to improve the diagnosis of HeV infection [47] .

Both are closely related, arthropod-born, 40e70 nm ss-RNA flaviviruses of the Flaviviridae family that may affect the central nervous system of horses [48, 49] .

Evidence of infection with NiV was found in the brain of one horse in which inflammation of the meningeal blood vessels occur [42] . NiV emerged in Malaysia, spread rapidly through the pig population, and caused the deaths of over 100 people [39] .

SalV has been recently identified in horses [50] . The only known isolate was obtained from a horse that was involved in a disease outbreak of undetermined nature [51] .

VSV is a 50e380 nm ss-RNA virus of the Rhabdoviridae family. It may cause stomatitis in horses, and may represent an emerging equine infectious disease [52] . An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) has been developed for the detection of primary infection of VSV in equine and swine sera [53] .

WNV is a 40e70 nm ss-RNA arbovirus of the Flaviviridae family, first identified in the West Nile district of Uganda in 1937. WNV is found over a broad geographical range and in a wide diversity of vertebrate host and vector species [54] . Until 1999, the disease was found in African, European, and Eurasian countries.

More recently, there was an increase in outbreaks of illness due to WNV in animals as well as humans and numerous cases are currently reported in Canada and the USA. Infected humans may be asymptomatic and the transfusion of donated blood from such individuals has resulted in the infection and death of recipients. This has led to the introduction in 2003 of strict regulatory measures in Canada and the USA which include the testing by a PCR assay of all blood donations. WNV primarily circulates between birds and mosquitoes, while mammalian infections are incidental. Mammal biting mosquitoes become infected when they feed on the blood of an infected bird. Once this happens, people, horses and other mammals may get WNV. Infected horses are, however, unlikely to serve as important amplifying hosts for WNV in nature [55] . Many WNV-infected horses probably never show symptoms, but a study reported mortality rates close to 30% [56] . Early symptoms are often indistinguishable from other equine encephalitides including rabies, equine herpesvirus-1, equine protozoal myeloencephalitis, and eastern, western, or Venezuelan equine encephalomyelitis. A vaccine for horses has been developed, but one horse that received two doses died from the disease [56] .

ERAV and ERBV are picornaviruses reclassified as members of the Aphtovirus genus because of resemblance to foot-and-mouth disease virus. They are 22e30 nm ss-RNA viruses. High neutralizing antibody titers develop and appear to correlate with strong reactivity to VP1 in Western blots [57] . A new serotype of the genus Erbovirus, tentatively named ERBV2 has been identified recently and found in 24% of horses in Australia [58] .

EEV is a 80 nm ds-RNA virus of the Reoviridae family. Several serotypes have been identified in southern Africa. Serotype-specific virus-neutralizing antibody in serum samples from horses suggests a widespread occurrence of infection but there seems to be only a low level of cross-protection in horses to natural reinfection [59] .

ERV is a 60e80 nm double-stranded RNA of the Reoviridae. Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. A PCR test with a detection limit of approximately 1.6 ! 10 2 TCID50 per gram faeces, with possible increase in the sensibility by one order of magnitude using nested PCR, was developed, representing a possible diagnostic tool [60] .

Antivenoms are produced from the plasma or serum collected from immunized horses or sheep. Plasma can be obtained by the centrifugation of whole blood or by apheresis. Plasma from several animals is typically pooled into 20e300 l batches and subjected to a fractionation process to isolate the IgG fraction. Following the initial use of crude equine immune serum, still reported to be manufactured by one producer [5] , various methods of IgG purification and refinement have been introduced ( Table 2 ). Most manufacturers use protocols derived from the basic method described by Pope [61, 62] , modified by Harms [63] , based on pepsin digestion at low pH, to obtain F(ab#) 2 fragments, followed by ammonium sulphate precipitation of antibody fragments [5, 64, 65] . This basic approach is combined with a number of additional steps, aimed at obtaining a purer [66] preparation, such as heat coagulation [64] and ion-exchange chromatography [66, 67] . The pH at which pepsin digestion is performed by various laboratories ranges between 3.1 and 3.8, at temperatures between 30 and 37 C. Pepsin digestion is usually performed in undiluted serum or plasma (protein concentration: 60e90 g/l). Incubation times range from 40 min to 24 h and with varying pepsin concentrations [64, 65, 67, 68] . Thermocoagulation is used by many laboratories and consists of heating at 55e56 C for 1 h [64, 65, 68] although not all methods involving pepsin digestion include heat coagulation [67] . Some fractionation protocols include an additional Table 2 Steps used for the manufacture of antivenoms Screening of production animals for adventitious agents Plasma collection (whole blood or apheresis) in bags or bottles Plasma thawing at room temperature Plasma pooling IgG/fragments purification process F(ab#) 2 : B pepsin digestion (acid pH) and ammonium sulphate precipitation, or B pepsin digestion (acid pH) and caprylic acid precipitation Fab: ammonium sulphate precipitation and papain digestion at pH 7e8 Whole IgG: B caprylic acid precipitation B ammonium sulphate precipitation IgG/fragments concentration: (NH 4 ) 2 SO 4 /Na 2 SO 4 precipitation, ultrafiltration Polishing: ion exchange (removes Fc and further purifies IgGs/ fragments) Ultrafiltration Sterile filtration Aseptic filling Storage in the liquid state or lyophilisation acidification step to remove some non-IgG globulins ('euglobulins') which are unstable at acid pHs [66, 69] . One producer includes a bulk pasteurisation step in its antivenom production protocol [66] .

Other manufacturers produce whole IgG antivenoms using either ammonium sulphate precipitation of IgG or caprylic acid precipitation of non-IgG proteins [69e71], followed by dialysis or ultrafiltration. Recently, a simple, one-step methodology based on caprylic acid precipitation has been described [72] . The conditions used for caprylic acid fractionation of equine plasma are as follows: plasma pH is adjusted to 5.8 and caprylic acid is added directly to this plasma to attain a final concentration of 5% (v/v). The mixture is stirred during caprylic acid addition and then for one additional hour, after which the precipitate is separated by filtration. Caprylic acid is then removed by either dialysis or ultrafiltration and the product is formulated before sterile filtration [72] . Some antivenoms are made of Fab antibody fragments, obtained by papain digestion of sheep IgG, at neutral pH, after sodium sulphate precipitation of IgG [73] . Affinity-chromatography or ion-exchange chromatography steps have been described in the production of some Fab antivenoms [74, 75] . Treatment with b-propiolactone has been evaluated to reduce complement activation by horse plasma-derived products [76] ; however, it does not appear to be used routinely in laboratories producing antivenoms.

In addition to the differences in fractionation protocols, antivenoms made of whole IgG, F(ab#) 2 and Fab molecules greatly differ in their pharmacokinetic profiles [1, 77] . Most antivenoms come in liquid presentation, but some are lyophilized. The former need to be stored at 4e8 C, since higher temperatures reduce their shelf-life and induce protein denaturation and aggregate formation [78] . In contrast, lyophilized antivenoms can be stored at room temperature and have a more prolonged shelf-life. Most antivenoms contain preservatives to prevent bacterial contamination [5, 79] .

Considerable experience has been gained in recent years on the viral safety of human IgG preparations and other human plasma-derived products. Historical perspectives on these products help in assessing the viral safety of antivenoms. The safety of human plasma products results from the combination of overlapping strategies that include: (a) selection of donors, (b) viral testing procedures on single donations (e.g. serological tests) and plasma pools (e.g. nucleic acid tests) to exclude donations contaminated by viruses, (c) processing and purification steps to inactivate and remove viruses, and (d) implementation of good manufacturing practices [80] . Whenever feasible, similar approaches should be used for the manufacture of animal plasmaderived products.

Monitoring the health of animals used for antivenom production is important [5] . Colonies of animals should be kept free of contamination through good husbandry or vaccination, where appropriate. Surveillance of the source animals should include screening for pathogens, health monitoring, including routine blood chemistry and haematology tests, and post-mortem examination. Ideally, animals should be kept under strictly contained conditions, but this is not readily applicable to horses. However, for instance, animals could be kept free of a particular arthropod-borne virus if they are maintained in an area free of the particular arthropod vector. If a particular pathogen is identified, it should be established whether it is present in plasma. Some virological screening methods may be performed, if available and epidemiologically relevant to a particular geographic region, to limit risk of virus presence in the animal herd and/or the plasma pool. To some extent the Guidelines from the Federation of European Laboratory Animal Science Association (FELASA), although not referring to horses, may serve as a reference [81e83]. Nevertheless, not all of these measures are available in many developing countries nor readily applicable to horses used for antivenom production, and pathogens can escape this surveillance program. This illustrates the need for manufacturing techniques to ensure a sufficient margin of safety with regards to potential infectious agents present in the manufacturing plasma pool. This also strongly emphasizes the crucial importance of ensuring an appropriate traceability system from horse donors to human recipients of antivenoms so that any potential infectious events or risks may be identified in a proper and timely manner and relevant counter-measures taken promptly.

Confidence in the safety of antivenoms must come from evidence that the manufacturing processes used can reproducibly remove or inactivate high potential levels of viruses. Comparison with human plasma fractionation suggests that some of the steps of antivenoms include treatments which may inactivate viruses. Human plasma fractionation is a complex integrated manufacturing process from which products like albumin, coagulation factors and IgG, are obtained. Purification processes combine cryoprecipitation, ethanol fractionation, chromatography, and ultrafiltration [84] . In addition, various types of dedicated viral reduction treatments have been introduced, most often on purpose, such as chemical treatments by solvent-detergent, caprylic acid precipitation, low-pH incubation, heat treatments in the liquid or in the dry states, and nanofiltration [80] . Among those, caprylic acid and low pH treatments, both of which are commonly used also for the purification of antivenom IgG, have been shown to contribute to the viral safety of human plasma IgG products as described below.

Some years ago, unsaturated fatty acids were shown experimentally to inactivate more than 4 log of lipidenveloped viruses (VSV, Sindbis virus, HIV) in human plasma protein fractions [85] . However, specific attention has been given to caprylic acid (also called octanoic acid), as it has been used as precipitating agent for human IgG. It has been suggested that the non-ionized form of the caprylic acid disrupts the integrity of the lipid bilayer and membrane associated proteins of enveloped viruses. Utilizing the dissociation reaction and varying the concentration of the ionized form of caprylate, a specific amount of the nonionized form of caprylate can be maintained over a wide pH range. Treatment of plasma protein solution with caprylate at acidic pH also readily inactivates lipid-enveloped viruses like herpes simplex virus type I, vesicular stomatitis virus, vaccinia virus, and Sindbis virus [86] .

Detailed validation studies of two caprylic acid treatments of human Ig have been published recently. The robustness of a treatment applied to three intravenous immunoglobulin preparations (IgG, IgM-enriched, and IgM preparations) has been investigated using HIV, BVDV, Sindbis virus and pseudorabies as model viruses [87] . The routine treatment conditions for these two IgG and this IgM preparation are indicated in Table 3 . Kinetics of inactivation was determined over a period of 1 h of treatment. Complete inactivation (corresponding to more than 4.68e6.25 log) is achieved within the first minutes. Within a certain range, viral inactivation in the IgG product was not affected by pH (5.5e6.0), temperature (0e26 C), and protein content (30e40 g/l). Above pH 6, and most specifically at pH 8, no BVDV inactivation was found. At a content of caprylic acid of 3.7 g/kg or less, inactivation of HIV is significantly reduced. Under the conditions applied during manufacture, caprylic acid leads to robust inactivation of lipid-enveloped viruses; pH is a particularly critical parameter and should be less than 6. These conditions have also been found to inactivate O4.7 log10 EAV, an equine virus used as a model (Dichtelmu¨ller, personal communication). Another study reported the viral inactivation achieved during caprylic acid precipitation of non-IgG proteins from human IgG product [88] . At pH 5.1, 23 C, and in the presence of 9 mM caprylate, R 4.7 and R 4.2 log of HIV and PRV, respectively, were inactivated during the 1 h treatment, but only 1.5 log for BVDV was inactivated. At 12 mM caprylate, R 4.4 log of BVDV was inactivated within this time period. At pH 5.1, 24 C, 19 mM caprylate, and pH 5.1, 24 C, 12 mM caprylate, complete inactivation of BVDV and of HIV and PRV was achieved in less than 3 min. The authors also showed that 40 mM caprylate at pH 5.4 and 40 C in supernatant IV-1, an intermediate in albumin production, inactivated R 4.2 log BVDV almost instantaneously [88] .

The virucidal effect of caprylic acid has also been confirmed in human albumin solution, where it is used as a stabilizer for pasteurisation. Elevated temperature and low pH were found to be critical parameters to ensuring significant reduction in virus infectivity. Rate and extent of inactivation were sensitive to variations in the caprylate to protein ratio and to changes in pH. In the conditions retained, 10% w/v protein, 16 mM caprylate, pH 4.5 and 30 C, more than 4 log inactivation of the lipid-enveloped viruses tested, including BVDV, was achieved [89] .

It should be kept in mind that treatment of whole plasma or crude fractions, as is the case for equine antivenoms production, may lead to lower rate and kinetics of viral inactivation, due to the high endogenous lipid content, as found in a study that evaluated the virucidal effect of sodium oleate [85] . Finally, one should keep in mind that caprylic acid treatment does not inactivate non-enveloped viruses.

Several human IgG preparations are subjected to an acid pH incubation that was historically introduced to reduce immediate adverse reactions subsequent to intravenous injections. In the late 1980s, acid pH treatment was also shown to contribute greatly to the Table 3 Comparison of conditions for caprylic acid treatment used for human IgG preparations (87) [90, 91] . The treatment of human IgG generally consists of incubation at pH 4, with or without traces of pepsin, at temperatures from 30 to 37 C, using a protein content close to 50 g/l, and for more than 20 h. The treatment is intended to eliminate aggregates. Pepsin, when present, is maintained at low concentration to avoid cleavage of the IgG molecule. Several model enveloped viruses were shown to be inactivated under those conditions [92e94]. More than 5e8 log inactivation of HIV and other enveloped viruses used as surrogate models, such as Semliki forest virus (SFV), HSV, VSV, and CMV is achieved. By contrast, poliovirus, as other non-enveloped virus, seems more resistant. The presence of trace concentrations of pepsin added to reduce anticomplementary activity during this procedure has been shown to contribute little to virus kill of most processes [95] . Virus inactivation by acid pH and pepsin is influenced by several parameters. HIV, BVDV, SFV, and PRV are completely inactivated within 5 mine1 h at 37 C but inactivation rate and extent is less at lower temperatures. Increasing the sucrose content from 0 to 15% reduced the rate of inactivation of PRV but not that of SFV. Increasing the NaCl content from 0 to 150 mM reduced the rate of inactivation of SFV but that of PRV was unchanged. An increase in the IgG concentration from 0 to 10% speeds up the inactivation of PRV but decreases that of SFV. Therefore, temperature is a major parameter in the virucidal efficacy of pH-pepsin treatment and the impact of solute composition is virus-dependent [93] . Some fractionation protocols used in antivenom production include a low pH treatment to induce the precipitation of 'euglobulins', which are plasma globulins unstable under these conditions. This step was shown to remove between 2.8 and 3.5 log of EIAV, Sindbis virus, poliovirus and porcine parvovirus [66] . Virus reduction may occur by co-precipitation with the discarded 'euglobulins' rather than by an inactivation process [66] .

Pasteurisation is the treatment of a liquid protein fraction for 10 h at 60 C [96] and is being used in the production of at least one type of equine-derived antivenom [66] . Experience with human plasma products show that liquid heat treatment may inactivate both enveloped and non-enveloped viruses. Pasteurisation of albumin solutions is carried out in the final container in the presence of low concentrations of sodium caprylate alone or with N-acetyl tryptophan. Inactivation of Sindbis and EMC model viruses added to 5% albumin solution was achieved within 10 min treatment [97] . However, inactivation of some non-enveloped viruses is less [80] , and the process appears to be virus-specific.

Various coagulation factors, protease inhibitors, and intravenous IgG are pasteurised during the purification process. Most often, pasteurisation is performed in the presence of stabilizers, like amino acids, sugars, or citrate [97, 98] , to protect protein functionality, and limit molecular alterations and protein aggregation. Protein stabilizers are also known to stabilize viruses, however, further highlighting the need for validating the exact conditions of treatment used. Pasteurisation can inactivate viruses of different types, enveloped or nonenveloped, including HIV, HBV, HCV, and HAV [80, 96, 98, 99] . There are few published data on the inactivation of resistant model non-enveloped viruses like porcine parvovirus, SV 40 or Reovirus type 3 in plasma products [100, 101] . In most instances, inactivation of such viruses is limited to less than 2e4 log over the 10 h heating period. Heat treatment of whole human plasma at 50 C for 3 h, in the presence of a specific combination of stabilizers, inactivates more than 6 log of HIV, and more than 4 log of enveloped and nonenveloped model viruses [102] . Finally, it was demonstrated that heat treatments like pasteurisation and vapour heat treatment (as well as solvent-detergent and nanofiltration on 35 nm membranes) can inactivate/ remove more than 6 log of WNV [103] .

Chromatography is primarily used in human plasma fractionation as a downstream polishing step [104] . Ionexchange chromatography has been described for some antivenom products [66, 67] . Viral partitioning has been shown to occur during affinity and ion-exchange chromatography, but the mechanism of removal is not easy to predict and control [105] , making it difficult to consider these as robust viral removal steps.

Storage at pH 4.25, in the presence of a stabilizer, such as 10% maltose, for a minimum of 21 days at 20 C, yields aggregate-free and in vitro functionally active human IgG preparations [106] . Incubating at pH 4.25 for 21 days at 20 C caused a 10,000-fold decrease in BVDV infectivity and complete inactivation of 1000 chimpanzee infectious doses per millilitre of HCV [107] . With the exception of a recent report of a Fab ovine antivenom formulated at pH 4.0 [108] , to our knowledge, formulation at low pH is not commonly used for antivenoms but may be considered as a viral safety step, if it does not affect the biological activity, the general safety, and the clinical efficacy of the product.

Viral validation studies are small-scale experiments designed to provide an estimate of the overall viral reduction level achieved across the manufacturing processes and to identify steps and parameters that are critical for viral inactivation or removal. To perform such studies, as described in the Guidelines prepared by the CPMP, production intermediates are voluntarily spiked with known amounts of known viruses, using laboratory-scale mimicking of the production process, and the virus reduction factor is calculated by comparing the infectivity level before and after each individual process step evaluated. When appropriate, a cumulative viral reduction factor can be calculated and provides information on the viral safety margin of processes for a range of model viruses. Effective and robust individual steps typically achieve more than 4 log reduction in infectivity under a large range of production parameters. Viral validation studies are usually conducted with 3e6 model viruses differing in their structural features (presence of an envelope, RNA or DNA genetic material, size, degree of resistance). ''Relevant'' viruses, which are known potential contaminant of the starting plasma, should be used when possible. A list of existing laboratory adapted model viruses that could be used for validation studies of antivenoms manufacturing processes is shown in Table 4 . VSV and WNV that may contaminate horses appear of special relevance for process validation of equine products.

Dedicated viral inactivation and removal production steps should be implemented in a way to ensure the reproducibility in viral reduction and the absence of risks of downstream contamination. Examples of approaches used for the manufacture of human plasma products are provided in recent WHO guidelines [109] , and a CPMP note for guidance provides recommendation on production of immunsera [19] . Both can be used as reference by manufacturers of antivenoms.

Viral reduction equipment should have adapted specifications. For instance, equipment for bulk in process virus inactivation, such as the acid pH incubation or the caprylic acid treatment, should ideally be fully enclosed. In addition, vessels should have temperature controls and be designed to avoid ''dead points'' where important parameters such as temperature and pH could not be ensured. Similarly, liquid heat treatment or heat shock should be conducted in jacketed tanks with the solution stirred throughout the heat cycle to ensure homogeneity and all points in the tank should be within the specified temperature range. During the qualification phases, the equipment and the required services should be shown to conform to the predefined technical specifications and requirements, and to function within the specified limits. When possible, it is recommended to create a dedicated ''viral safety area'' where production steps are arranged in a clear and logical way, so that recontamination of the virally reduced intermediates is avoided. Operating procedures should also be written to reduce the likelihood of crosscontamination. Bulk pH 4 or caprylic acid virus inactivation could be carried out in two stages, the first stage being located in a normal production room, followed by a second incubation in another tank located in a segregated, contained area. The duration of the first stage should be such that the majority of virus inactivation (as found during the viral validation studies) has occurred. On completion of the first stage, the product should be aseptically transferred (e.g. through sterile coupling) into the second vessel located in a safety zone for completion of the second stage of viral inactivation. Product-contacting-equipment used in the safety area should be dedicated or decontaminated to inactivate any remaining virus. A quality assurance system should ensure that the execution of virus reduction methods conforms to the validated conditions. Viral inactivation and removal procedures should be described in approved Standard Operating Procedures (SOPs) that contain critical process limits for the viral inactivation and removal methods applied to antivenoms. 

To date there is no documented example of viral transmission to humans from the use of antivenoms, whichever type of product (whole IgG, F(ab#) 2 or Fab) is manufactured [5] . Considering the difficulty in the containment of large animals like horses, and in an exhaustive viral testing of the starting plasma, the viral safety of antivenoms appears therefore largely dependent upon the capacity of manufacturing processes to reduce infectious agents and on the use of good manufacturing practices, in particular in ensuring production traceability. By contrast to human plasma products, most antivenoms are currently not subjected to purposely introduced viral reduction steps like solvent-detergent, pasteurisation, or nanofiltration [80, 109, 110] . However, a comparison with validated manufacturing processes used for human IgG clearly indicates that at least two widely used antivenom production steps, caprylic acid treatment and low-pH incubation, are likely to contribute in a robust manner to viral safety, at least against enveloped viruses. The concentration of caprylic acid (5%) used for antivenoms is higher than that used for human IgG (Table 3) , and the pH of treatment is within the effective range (less than pH 6) required for optimal inactivation of enveloped viruses. As the treatment is performed on equine plasma itself, or on crude fractions, the viral kill could be reduced by the presence of lipids. The acid incubation of antivenoms which is performed at a pH (3.1e3.8), less than that used for human IgG, and at a similarly warm temperature (30e37 C) ( Table 5) should provide a fast rate of inactivation of lipidenveloped viruses, and possibly also non-enveloped viruses. However, the incubation time (40 mine24 h) is for some processes significantly less than for human IgG (typically 22e25 h), probably reducing the extent of viral kill achieved. Preliminary viral kill data obtained with antivenoms confirm that conditions used for peptic cleavage at low pH of F(ab#) 2 achieve robust inactivation of WNV [111] . We recommend that further representative viral validation studies using a range of well-established virus models are performed to know the rate and extent of viral kill actually achieved during those production steps. The viruses to use for those validations should be discussed with specialized virologists, and, by analogy with most studies done with human plasma derivatives, might include three lipidenveloped viruses (such as BVDV, VSV, PSR, and WNV) and one non-enveloped virus (such as EMC, poliovirus, or Reovirus 3). Inactivation data could then be compared to viral kills achieved using similar process steps of human IgG products, therefore providing some reference on their robustness and allowing manufacturers and national regulatory authorities to assess scientifically the margin of safety of antivenom products. Other manufacturing steps, such as heat coagulation, could be validated as well. Formulation of the product at low pH, as done for some human IgG preparations, is another potential approach to consider for improved viral safety, but evaluating product stability and efficacy under such conditions would be a pre-requisite. In view of their expected beneficial impact on viral safety, appropriate process implementation of caprylic acid treatments and low-pH incubations should be ensured, as well as measures taken to avoid risks of downstream contamination and, more widely, to respect good manufacturing practices and traceability concepts. Such conclusions should also be valid for other animal plasma-derived IgG products used in human medicine such as anti-lymphocyte/T-cell, antitoxins, and anti-bacterial or viral agents sera [19] . 

Snake antivenoms

Marine antivenoms

Venomous bites and stings

A new Pan African polyspecific antivenom developed in response to the antivenom crisis in Africa

Report of a WHO workshop on the standardization and control of antivenoms

Venoms, antivenoms and immunotherapy

Efficacy, safety, and use of snake antivenoms in the United States

Antivenoms: a list of hyperimmune sera currently available for the treatment of envenoming by bites and stings

Handbook of clinical toxicology of animal venoms and poisons

Snake-bites: appraisal of the global situation

Clinical toxicology of snakebite in Central America

Handbook of clinical toxicology of animal venoms and poisons

Development of an improved method for production of antiscorpion F(ab#)2 fragment of IgG with high yield and potency

Antivenom treatment in arachnidism

Emergence of transmissible disorders, a continuous processda new type of viral meningoencephalitis

Recent developments in the epidemiology of virus diseases

Possible role of an animal vector in the SARS outbreak at Amoy Gardens

Biologists seek to head off future sources of infection

Note for guidance on production and quality control of animal immunoglobulins and immunsera for human use. London: European Agency for the Evaluation of Medicinal Products (EMEA)

Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole

Growth characteristics of a highly virulent, a moderately virulent, and an avirulent strain of equine arteritis virus in primary equine endothelial cells are predictive of their virulence to horses

Detection of equine arteritis virus by real-time TaqMan reverse transcription-PCR assay

Stable and long-lasting immune response in horses after DNA vaccination against equine arteritis virus

Borna disease: current knowledge and virus detection in France

Borna disease virus and infection in humans

Borna disease virus: new aspects on infection, disease, diagnosis and epidemiology

Clinical, pathologic, immunohistochemical, and virologic findings of eastern equine encephalomyelitis in two horses

Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays

Immune response of horses after simultaneous or sequential vaccination against eastern, western, and Venezuelan equine encephalomyelitis

Eastern equine encephalomyelitis virus infection in a horse from California

Characterization of a coronavirus isolated from a diarrheic foal

Further characterization of equine foamy virus reveals unusual features among the foamy viruses

Detection and isolation of equine herpesviruses 1 and 4 from horses in Normandy: an autopsy study of tissue distribution in relation to vaccination status

Equine herpesvirus 1 and 4 infections: an update

Detection of equine herpesvirus type 2 (EHV-2) in horses with keratoconjunctivitis

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Development of a multiplex real-time reverse transcriptase-polymerase chain reaction for equine infectious anemia virus (EIAV)

Equine influenza virus infections: an update

The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae

Newly discovered viruses of flying foxes

Transmission studies of Hendra virus (equine morbillivirus) in fruit bats, horses and cats

Hendra and Nipah virus infections

The Australian paralysis tick may be the missing link in the transmission of Hendra virus from bats to horses to humans

Infection of humans and horses by a newly described morbillivirus

Hendra virus disease in horses

Hendra (equine morbillivirus)

Development of a fluorogenic RT-PCR assay (TaqMan) for the detection of Hendra virus

Japanese encephalitis in horses in Japan

Japanese B encephalitis in a horse

Identification and phylogenetic comparison of Salem virus, a novel paramyxovirus of horses

Isolation of Salem virus, a novel equine paramyxovirus, and assessment of its etiologic role in a disease outbreak

Vesicular stomatitis and other vesicular, erosive, and ulcerative diseases of horses

Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus

West Nile virus in livestock and wildlife

Experimental infection of horses with West Nile virus

West Nile virus encephalomyelitis in horses: 46 cases

Equine rhinitis A virus: structural proteins and immune response

Equine rhinitis B virus: a new serotype

The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa

Nested reverse transcriptase-polymerase chain reaction for the detection of group A rotaviruses

The action of proteolytic enzymes on the antitoxins and proteins in immune sera. I. True digestion of the proteins

The action of proteolytic enzymes on the antitoxins and proteins in immune sera. II. Heat denaturation after partial enzyme action

The purification of antitoxic plasmas by enzyme treatment and heat denaturation

Antivenins in Brazil: preparation

Antivenin production in India

Preparation of improved F(ab#)2 antivenoms. An example: new polyvalent European viper antivenom (equine)

A protocol for 'enhanced pepsin digestion': a step by step method for obtaining pure antibody fragments in high yield from serum

Quantitative comparison on the refinement of horse antivenom by salt fractionation and ionexchange chromatography

Manual de Procedimientos. Produc-cio´n y Pruebas de Control en la Preparacio´n de Antisueros Difte´rico

Purification of F(ab#)2 anti-snake venom by caprylic acid: a fast method for obtaining IgG fragments with high neutralization activity, purity and yield

A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms

Caprylic acid fractionation of hyperimmune horse plasma: description of a simple procedure for antivenom production

A new antivenom to treat eastern coral snake (Micrurus fulvius fulvius) envenoming

First clinical experiences with specific sheep Fab fragments in snake bite

First clinical experiences with a new ovine Fab Echis ocellatus snake bite antivenom in Nigeria: randomized comparative trial with Institute Pasteur Serum (Ipser) Africa antivenom

Effect of beta-propiolactone treatment on the complement activation mediated by equine antisera

Pharmacokinetic-pharmacodynamic relationships of immunoglobulin therapy for envenomation

Effect of storage temperature on the stability of the liquid polyvalent antivenom produced in Costa Rica

Effect of preservatives on IgG aggregation, complementactivating effect and hypotensive activity of horse polyvalent antivenom used in snakebite envenomation

Reducing the risk of infection from plasma products: specific preventative strategies

Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units

FELASA recommendations for the health monitoring of experimental units of calves, sheep and goats Report of the federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Health

Report of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Health accepted by the FELASA Board of Management

Chromatography in plasma fractionation: benefits and future trends

Inactivation of lipid-enveloped viruses in labile blood derivatives by unsaturated fatty acids

Inactivation of lipid-enveloped viruses in proteins by caprylate

Inactivation of lipid enveloped viruses by octanoic acid treatment of immunoglobulin solution

Enveloped virus inactivation by caprylate: a robust alternative to solvent-detergent treatment in plasma derived intermediates

Low pH, caprylate incubation as a second viral inactivation step in the manufacture of albumin. Parametric and validation studies

Potential contribution of mild pepsin treatment at pH4 to the viral safety of human immunoglobulin products

The viral safety of intravenous immune globulin

Virus validation of pH 4-treated human immunoglobulin products produced by the Cohn fractionation process

Virus inactivation by pepsin treatment at pH 4 of IgG solutions: factors affecting the rate of virus inactivation

Virus inactivation during intravenous immunoglobulin production

Viral safety of intravenous immunoglobulins G for therapeutic use

Strategies to produce virus-safe blood derivatives

Inactivation of viruses in labile blood derivatives. II. Physical methods

Pasteurization as an efficient method to inactivate blood borne viruses in factor VIII concentrates

Inactivation of HIV, HBV, HCV related viruses and other viruses in human plasma derivatives by pasteurisation

A pasteurized therapeutic plasma

Pasteurization of antihemophilic factor and model virus inactivation studies

Virucidal heattreatment of single plasma units: a potential approach for developing countries

Nile virus and the safety of plasma derivatives: verification of high safety margins, and the validity of predictions based on model virus data

Affinity chromatography in the industrial purification of plasma proteins for therapeutic use

Chromatographic removal of viruses from plasma derivatives

A comparative in vitro study of low pH and enzyme treated immunoglobulin preparation for intravenous use

Inactivation of hepatitis C virus in low pH intravenous immunoglobulin

Formulation of a liquid ovine Fab-based antivenom for the treatment of envenomation by the Nigerian carpet viper (Echis ocellatus)

World Health Organization Expert Committee of Biological Standardization. Guidelines on viral inactivation and removal procedures intended to assure the viral safety of human blood plasma products. www.who.int/Biologicals-Quality%20Assur-ance%20and%20Safety

Nanofiltration of plasma-derived biopharmaceutical products

Inactivation of West-Nile virus during peptic cleavage of horse plasma IgG

We express our sincere thanks to Dr. Herbert Dichtelmu¨ller for his review of the manuscript and expert suggestions.
