key: cord-274141-vujx538o
authors: Chinsembu, Kazhila C.
title: Coronaviruses and Nature’s Pharmacy for the Relief of Coronavirus Disease 2019
date: 2020-10-06
journal: Rev Bras Farmacogn
DOI: 10.1007/s43450-020-00104-7
sha: 
doc_id: 274141
cord_uid: vujx538o

[Image: see text]

Coronaviruses (CoVs) cause acute and chronic respiratory, enteric, and central nervous system diseases in many species of animals including humans. While the history of CoVs dates as far back as the 1940s, identification of the first human CoVs (HCoVs) as causative agents of mild respiratory infections was reported in the 1960s. These HCoVs were named human CoV 229E (HCoV-229E) and HCoV-OC43 (Pillaiyar et al. 2020) . The replication and pathogenesis of CoVs were actively studied by virologists in the 1970s, leading to the discovery of four other human CoVs, namely HCoV-Hong Kong University 1 (HCoV-HKU1), human coronavirus-NL63 (HCoV-NL63), severe acute respiratory syndrome (SARS)-CoV, and Middle East respiratory syndrome (MERS)-CoV (Pillaiyar et al. 2020) .

SARS is a highly contagious and often fatal respiratory infection. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are ubiquitous and cause approximately onethird of common cold in humans. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 strains are usually associated with mild, self-limiting upper respiratory tract infections, such as the common cold (Andersen et al. 2020) . However, subtypes HCoV-229E, HCoV-OC43, HCoV-HKU1, and HCoV-NL63 can cause life-threatening pneumonia and bronchitis in the elderly and immunologically compromised individuals including those undergoing chemotherapy and persons living with HIV/AIDS. Indeed, before the discovery of SARS-CoV, two coronaviruses, HCoV-229E and HCoV-OC43, were known to infect humans, but they caused only selflimiting upper respiratory tract infections (30% of the common colds), and had never been reported to cause severe illness (Kuba et al. 2005) . SARS-CoV caused a global epidemic from November 2002 to 2003; about 8422 people were infected in 32 countries, 916 persons died, and the fatality rate was 10-15%. Analysis of the full sequence of SARS-CoV, which is about 29.7 kb in length, reveals that it is a large, single-stranded, positivesense RNA virus .

In 2013, MERS-CoV also triggered an epidemic in the Middle East and a major outbreak in South Korea occurred in May-June 2015 (Kim et al. 2017a) . With a case-fatality rate of 35.5%, MERS-CoV is heavily endemic in dromedary camels and causes lower respiratory tract infections in humans (Kim et al. 2017a) . Although the SARS-CoV epidemic had been under control for years, Wang et al. (2017) warned that re-emergence of this threatening infection posed a possible global risk as potentially new strains of SARS-CoV would be more dangerous than the previous ones.

Toward the end of 2019 and the beginning of 2020, multiple human cases of a new viral infection were reportedly linked to the Huanan Seafood Wholesale "wet" Market (South China Seafood City Food Market) in Wuhan, China Zhang et al. 2020 ). On 7 January 2020, the new virus was identified as a novel coronavirus and officially named by the World Health Organization as 2019-nCoV ). On 20 January 2020, the National Health Commission of China confirmed the human-to-human transmission of 2019-nCoV with its presenting common symptoms such as fever, tiredness, dry cough, shortness of breath, and pneumonia (Elfiky 2020) , and less common signs as diarrhea, conjunctivitis, headache, loss of taste or smell, and rash on skin or discoloration of fingers and toes. The World Health Organization also recognized 2019-nCoV as the causative agent of the coronavirus disease 2019 . The 2019-nCoV was later named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the International Committee on Taxonomy of Viruses, Coronaviridae Study Group (Ling 2020) .

A phylogenetic analysis found a bat origin for the SARS-CoV-2 (Lu et al. 2020; Wan et al. 2020) . Yang et al. (2020) reported that SARS-CoV-2, formerly known as 2019-nCoV, the causative pathogen of COVID-19, had rapidly spread across China and around the world, causing an outbreak of acute infectious pneumonia. The spread of SARS-CoV-2, a new highly contagious and lethal strain of HCoVs, has now caused a global pandemic (Remuzzi and Remuzzi 2020) . In the morning of 7 September 2020, Johns Hopkins University reported that there were over 26,994,442 total confirmed cases and 880,994 deaths globally; over 6,189,488 cases and 187,541 deaths were in the United States of America (USA). COVID-19 has spread more rapidly due to increased global travel and adaptation of SARS-CoV-2 in every environment (Vellingiri et al. 2020) .

Currently, the seven identified human coronaviruses (HCoVs) are SARS-CoV-2, MERS-CoV, SARS-CoV, HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 strains (Andersen et al. 2020 ). Molecular evolutionary analysis shows that all HCoVs originate in animals; SARS-CoV, MERS-CoV, HCoV-NL63, and HCoV-229E are derived from bats; HCoV-OC43 and HCoV-HKU1 are sourced from rodents (Forni et al. 2017) . The 229E and NL63 strains of HCoVs belong to alpha coronaviruses while OC43, HKU1, SARS, MERS, and SARS-CoV-2 belong to beta coronaviruses (Elfiky 2020) . Most people infected with SARS-CoV, MERS-CoV, and SARS-CoV-2 present with severe respiratory distress, and many of the infected succumb to coronavirus infection (Andersen et al. 2020) . Pillaiyar et al. (2020) reviewed recent developments in the discovery and development of synthetic drugs to treat HCoVs. However, these drugs have not achieved extended distribution for clinical application because they have not yet been tested in rigorous randomized controlled clinical trials (Ford et al. 2020) . Zhou et al. (2020a, b) reported that there were no effective drugs targeting SARS-CoV-2. Yang et al. (2020) also stated no efficacious and safe antiviral drugs or vaccines were available for the treatment of this sudden and lethal disease. Supportive care and non-specific treatment to ameliorate COVID-19 symptoms of the patient are the main options available.

Remdesivir, produced by the company Gilead, shows EC 90 of 1.76 μM against SARS-CoV-2 in vitro (Elfiky 2020). On 1 May 2020, remdesivir was granted approval by the US Food and Drug Administration for emergency use in the treatment of COVID-19 patients. An inhibitor of the enzyme RNAdependent RNA polymerase (RdRp) involved in HCoV replication, the drug reduced mortality from 11 to 8% and duration of symptoms from 15 to 11 days. However, in comparison with the placebo, remdesivir's reduction of patient mortality was not significantly different.

Due to the absence of conventional therapies, Yang et al. (2020) reported that more than 85% of SARS-CoV-2-infected patients in China were treated with traditional Chinese medicine (TCM). During the 2002 to 2003 outbreak, TCM was also used as a supplementary treatment of severe laboratoryconfirmed conditions of SARS (Hsu et al. 2006a) . Along this trajectory, the aim of the current review is to provide an update on the bioactivity functions of medicinal plants and other natural products that have previously been used to treat coronaviruses. In the short term, knowledge of these natural products may be significant in the relief of COVID-19 symptoms. In the long term, natural products that alleviate COVID-19 symptoms may be used in the search for novel drugs against SARS-CoV-2. In the face of current challenges to the treatment of COVID-19, data presented in this review may help to reimagine a new future where natural products are the new frontier for more efficacious and low-cost SARS-CoV-2 inhibitors. The goal is to create a strong bioprospecting pipeline from which pharmaceutical companies can advance the discovery and repurposing of COVID-19 drugs from current medicinal plants and other natural products.

To prevent author bias, this review was carried out using a comprehensive and systematic data mining approach similar to that used by Chinsembu (2019) . To obtain pertinent literature, the key words "SARS" and "medicinal plants" were concomitantly searched in Google Scholar, Elsevier's payto-view service Science Direct, Scopus, Scielo, and PubMed Central, the US National Library of Medicine's digital archive of biomedical and life sciences journal literature. Literature sources included peer-reviewed journal articles and refereed books. Medicinal plants, mushrooms, algae, and sponges with anti-SARS activity were included. Wherever possible, active compounds and anti-SARS profiles mainly half maximal inhibitory concentration (IC 50 ) and effective concentration (EC 50 ) were recorded. Inclusion criteria included plants, fungi, and marine organisms, namely algae and sponges. Exclusion criteria included animal products. Countries reported in the review were those where primary data were collected; hence, geographical scope was not deliberately selected. The International Plant Names Index (http://www.ipni.org) and The Plant List (http://www.theplantlist.org) were used to verify names of plant species.

The spike protein, a type I membrane-bound protein, is essential for SARS-CoV attachment to the host cell receptor angiotensin-converting enzyme 2 (ACE2). In general, SARS-CoV entry starts with the attachment of the virus to its specific receptor ACE2 on the host cell surface (Keyaerts et al. 2007 ). Binding of ACE2 then induces the viral envelope protein to undergo conformational changes that mediate fusion between SARS-CoV and host cell membranes. The spike protein is responsible for these two steps in the coronavirus entry process (Keyaerts et al. 2007) . A member of the C-type lectin superfamily, dendritic cell (DC)-specific ICAMgrabbing non-intergrin (DC-SIGN), a molecule designated as CD209, binds to surface proteins of SARS-CoV, consequently facilitating viral entry (Liu et al. 2015) . SARS-CoV spike protein contains a number of N-linked glycosylation sites that modulate binding to the DC-SIGN receptor on DC (Zhang et al. 2007 ). SARS-CoV binding to DC-SIGN is required for DC infection and target cell trans-infection. DC-SIGN plays an important role in mediating DC adhesion and internalization of SARS-CoV. Antiviral agents from plants generally block virus entry and membrane fusion (Hsieh et al. 2004 ). De Clercq (2005 suggested that it was feasible to develop SARS-CoV fusion inhibitors analogous to enfuvirtide, a linear 36-amino acid synthetic peptide marketed under the trade name Fuzeon, an approved anti-HIV drug that inhibits the entry of the virus into cells. SARS-CoV entry inhibitors fall into several categories. The first group consists of inhibitors that bind to the ACE2 receptor, the second class comprises entry inhibitors that bind to the virus and prevent it from interacting with its receptors, and the third set of inhibitors can obstruct the conformational changes thus thwarting SARS-CoV fusion with the target cells (Keyaerts et al. 2007 ). An effective inhibitor of SARS-CoV, the medicinal basidiomycete Ganoderma lucidum (Curtis) Karst. contains ganoderic acid F, a lanostane triterpene that acts as an inhibitor of ACE2 receptor (IC 50 4.7 × 10 −6 M) (Morigiwa et al. 1986 ).

Lee (2019) asserts that lectins have emerged as a new class of antiviral biologic drugs or biologics by taking advantage of a unique glycosylation pattern only found on the surface of viruses. Lectins, a group of proteins with carbohydrate recognition activity, recognize the SARS-CoV spike protein (Liu et al. 2015) . Keyaerts et al. (2007) found prominent anti-SARS-CoV activity among mannose-specific plant lectins. One of the most potent lectins against the SARS-CoVinduced cytopathicity is the mannose-specific plant lectin isolated from leek (Allium porrum L.) with an EC 50 of 0.45 μg/ml and a selectivity index of > 222. The N-acetyl glucosamine-specific lectins isolated from the stinging nettle (Urtica dioica L.) and from the tobacco plant (Nicotiana tabacum L.) were also markedly active against the SARS-CoV with a selectivity index of > 77 and > 59, respectively (Kumaki et al. 2011) . A mannose-specific lectin (agglutinin) of Hippeastrum striatum (Lam.) H.E.Moore prevented the penetration of SARS-CoV into Vero E6 cells (EC 50 2.5 μg/ml at 4°C) (Keyaerts et al. 2007 ). SARS-CoV spike protein contains 12 N-glycosylation sites and the sugars attached to four of these N-glycosylation sites have been identified (Zhou et al. 2010) . Two of the four sugars were found to be high-mannose-type glycans and the other two showed complex glycan structures . The potent anti-SARS-CoV activity of mannose-specific plant lectins is therefore attributable to the presence of high-mannose-type glycans in the SARS-CoV spike protein (Keyaerts et al. 2007; Zhou et al. 2010 ).

Many lectins from algae have antiviral activity. A highly potent broad-spectrum antiviral, griffithsin is a 121-amino acid red alga-derived lectin that binds to the terminal mannose residues of the asparagine(N)-linked Man 5-9 GlcNAc2 structures found on the envelope of SARS-CoV (Lee 2019). Initially identified as an anti-HIV-1 agent with EC 50 of 0.43-0.98 nM, griffithsin isolated from the aqueous extract of the red algae Griffithsia corallinoides (L.) Trevisan found off the eastern shore of Chatham Island, New Zealand, is a 12.7-kDa lectin that also inhibits SARS-CoV (EC 50 0.048-960 nM), various avian CoV subtypes (EC 50 0.032-0.57 nM), bovine CoV (EC 50 0.057 nM), puffinosis CoV (EC 50 0.57 nM), and HCoV mutants with EC 50 of 0.16 nM (Mitchell et al. 2017 ).

Some viruses such as Ebola and SARS-COV use cathepsins, a family of proteases, to infect host cells (Huang et al. 2006) . Inhibitors of cathepsin L prevent entry of SARS-CoV into target cells (Simmons et al. 2005) . Unfortunately, many cathepsin inhibitors are non-selective (Zhou et al. 2016) . Leupeptin (1), also known as N-acetyl-L-leucyl-L-leucyl-L-argininal, is protease inhibitor produced by actinomycetes and isolated from a strain of Streptomyces exfoliatus (Waksman and Curtis) Waksman and Henrici, which can inhibit cysteine, serine, and threonine peptidases. Simmons et al. (2005) showed that inhibitors of proteases such as leupeptin act as potent inhibitors of SARS-CoV entry into host cells. SARS-CoV spike protein-driven virus-cell fusion is inhibited by 1 , which exhibited a dose-dependent inhibition of SARS-CoV infection ) (IC 95 15.2 μM). This inhibition is the result of an absolute requirement in cell lines for endosomal processing of the SARS-CoV spike protein by cathepsin L during entry into the target cell ). The oligopeptide antipain (2), an inhibitor of trypsin and papain, isolated from actinomycetes inhibited SARS-COV entry into host cells (Simmons et al. 2005 ). (Dong et al. 2016; Zhou et al., 2020a, b) , and Cassia tora L. Emodin inhibited SARS-CoV via the blockage of viral entry by binding to the spike proteins and interfering with SARS-CoV 3CL pro activity (Hyun et al. 2009 ). In assays involving SARS-CoV and HCoV-OC43, emodin in a dose-dependent manner significantly blocked the interaction between the SARS-CoV spike protein and ACE2 (a functional SARS-CoV receptor), inhibited the 3a ion channel (K 1/2 value of about 20 μM), and stopped new coronavirus release (Dong et al. 2016; Ho et al. 2007 ). SARS-CoV-2 spike protein directly binds to the host cell surface ACE2 receptor facilitating virus entry and replication . Corollary, emodin can be considered a potential lead therapeutic agent in the treatment of SARS-CoV-2 infection. Experimental data now suggest that combining emodin and the drug toremifene, a first-generation non-steroidal selective estrogen receptor modulator for the treatment of metastatic breast cancer, provides a potential therapeutic approach for SARS-CoV-2 (Zhou et al., 2020a, b) .

Terpenoids isolated from medicinal plants have attracted attention because many of them exhibit specific in vitro antiviral effect against SARS-CoV (Wen et al. 2007 ). Triterpenoids inhibit the entry of Ebola, Marburg, HIV, and influenza A viruses with distinct structure-activity relationships (Si et al. 2018 ). These natural agents modulate a broad range of virus-host fusion functions via wrapping the heptad repeat 2 domain prevalent in viral envelopes. Euphorbia neriifolia L. produces 22 triterpenoids with stronger activity against HCoVs than actinomycin D (Chang et al. 2012) .

Saikosaponins represent a group of oleanane derivatives, usually as glucosides, found in medicinal plants in the genera Bupleurum L., Heteromorpha Cham. & Schltdl., and Scrophularia L. (Cheng et al. 2006) . Saikosaponins A, B 2 , C, and D demonstrated anti-HCoV-229E activity at concentrations of 0.25-25 μM, with the strongest activity being noted for saikosaponin B 2 (4) (IC 50 1.7 ± 0.1 μM) (Cheng et al. 2006 ). Since saikosaponin B 2 blocks HCoV-229E attachment and penetration, it could be a novel lead for the development of a potential chemo-preventive agent for SARS-CoV infection (Cheng et al. 2006) . Yang et al. (2020) reported that saikosaponins and glycyrrhizin from Toona sinensis (Juss.) M.Roem. had potent anti-SARS-CoV effects by inhibition of viral cellular entry, adsorption, and penetration.

The licorice plant, Glycyrrhiza glabra L., was reported to have specific anti-SARS effects (Polansky and Lori 2020) . Constituents of licorice include triterpenoids such as glycyrrhizin or glycyrrhizic acid (5). Glycyrrhizin (5) exerted very high antiviral activity and in vitro inhibition of SARS-CoV (EC 50 300 μg/ml) (Cinatl et al. 2003) . Glycyrrhizin and its derivatives inhibited SARS-CoV replication in vitro (Baltina et al. 2015) . Ryu et al. (2010a) reported that 95% methanolic extracts of the bark of Tripterygium regelii Sprague & Takeda remarkably inhibited SARS-CoV 3CL pro activity (> 70% inhibition at 30 μg/ml) (Ryu et al. 2010a) . The active compounds of T. regelii are four quinone-methide triterpenoid derivatives: celastrol, pristimerin, tingenone, and iguesterin (6) with IC 50 values of 10.3, 5.5, 9.9, and 2.6 μM, respectively, against SARS-CoV 3CL pro (Ryu et al. 2010a ).

The seeds of Aesculus turbinata Blume, a medicinal plant found in China, Japan, and South Korea, contain a natural saponins called escins, constituents with strong virucidal effects against SARS-CoV (EC 50 of 6 μM, selectivity index value of 2.5) (Kim et al. 2017b; Salinas et al. 2019) . Betulin and betulinic acid, the main antiviral components of the Betula L. plants, inhibit HIV fusion and one of the steps in HIV maturation (Chinsembu 2016a) . A methanolic extract of Betula papyrifera Marshall exerted antiviral activity against bovine coronavirus (Rastogi et al. 2015) .

Many natural polyphenols, with antioxidant properties, possess antiviral activity and some of them, for example, myricetin (7), an inhibitor of coronavirus helicase nsP13 (IC 50 value of 2.71 μM), are already being used against coronaviruses (Semwal et al. 2016; Yu et al. 2012) . Naturally occurring flavonoids such as quercetin, naringin, hesperetin, and catechin, the most abundant polyphenols in the human diet, are usually found in fruit and vegetables as glycosides and sometimes as acylglycosides. Luteolin and quercetin can interfere with adsorption of the virus to its host cells. It was hypothesized that specific flavonoids, such as quercetin, hesperetin, and myricetin (7) and their glycosylated derivatives, may play an effective role in inhibiting SARS-CoV entry into host cells, specifically by binding with high affinity to the spike protein, helicase, and protease sites on the ACE receptor (Ngwa et al. 2020) , in addition to acting against fatty acid synthase (Wen et al., 2011) . Extracted from TCM herbal formulae used to treat SARS, the following active polyphenols inhibited various steps during SARS-CoV entry and replication: baicalin, emodin, epigallocatechin, gallate, gallocatechin gallate, herbacetin, isobavaschalcone, kaempferol derivatives, luteolin, myricetin, quercetin, 3-β-D-glucoside, rhoifolin, pectolinarin, scutellarein, and tetra-O-galloyl-β-D-glucose ).

Quercetin (8) is an aglycone present at high concentration in onions. This compound has virucidal activity against enveloped viruses such as mengovirus, herpes simplex, parainfluenza type 3, pseudorabies, respiratory syncytial, and Sindbis viruses ). Quercetin is able to inhibit H + -ATPase of lysosomal membrane and thus prevents virus coat removal and blocks viral replication and competitively inhibits SARS-CoV 3CL pro with IC 50 of 42.79 ± 4.97 μM (Chen et al. 2006) .

Torreya nucifera (L.) Siebold & Zucc. is a traditional medicinal plant in Asia. The ethanol leaf extract of T. nucifera exerts good SARS-CoV 3CL pro inhibitory activity (62% at 100 μg/ml) (Ryu et al. 2010b ). Following the bioactivityguided fractionation of T. nucifera, the biflavone amentoflavone (9) showed the most potent SARS-CoV 3CL pro inhibitory effect with IC 50 of 8.3 μM (Ryu et al. 2010b) . Apigenin, luteolin, and quercetin inhibited SARS-CoV 3CL pro activity with IC 50 values of 280.8, 20.2, and 23.8 μM, respectively (Ryu et al. 2010b ). Zhong et al. (2013) found that Jinchai, a capsule of TCM consisting of plants such as Lonicera japonica Thunb., Bupleurum chinense DC., Astragalus membranaceus (Fisch.) Bunge, and Codonopsis pilosula subsp. tangshen (Oliv.) D.Y.Hong inhibited SARS-CoV. Jinchai blocks SARS-CoV infection by weakening adsorption of viruses to cells and reduces the ability of SARS-CoV to infect surrounding tissues (Zhong et al. 2013) . The main active constituents of Jinchai are chlorogenic acid and baicalin (10) whose EC 50 (μg/ml) at 48 h was 12.5 to 25, EC 50 (μg/ml) at 72 h was 25 to 50, CC 50 (μg/ml) was > 100, and SI calculated as CC 50 /EC 50 at 48 h was > 4 to 8 . Camellia sinensis (L.) Kuntze (black tea) polyphenol epigallocatechin gallate (EGCG) inhibited bovine coronavirus propagation; EGCG interfered with the adsorption of bovine coronavirus to Madin-Darby bovine kidney cells (Bansal et al. 2013) . Although Sanguisorba officinalis L. extract inhibits HIV-1 infection by binding to the viral envelope and blocking entry of the virus, it did not inhibit SARS-CoV entry (Liang et al. 2013) .

In oriental herbal medicine, the bark of cinnamon plant Cinnamomum cassia (L.) J. Presl has been used as a spice, infusion, and prime component of herbal remedies for common cold, gastrointestinal infections, cancer, chronic cardiovascular disease, and gynecological disorders. Zhuang et al. (2009) found that cinnamon bark extract has anti-RNA viral effects. The cinnamon extract inhibited wild-type SARS-CoV infection in vitro with an IC 50 of 43 μM; and the proposed mechanism of action for cinnamon bark extract was blocking cell entry via endocytosis (Polansky and Lori 2020) . Whereas the antiviral, anti-bacterial, and anti-cancer effects were attributable to essential oils such as cinnamaldehyde (11), the anti-inflammatory actions of cinnamon water extract were due to the presence of polyphenols such as flavonoids and tannins. Molecular docking studies show that 11 may block the attachment of SARC-CoV-2, though detailed in vitro and in vivo studies are required to confirm its efficacy (Asif et al. 2020) . Compound 11 had the lowest electronegativity value among the phytochemicals with a score of − 4.34; binding affinity in kilocalorie per mole was − 5 (Asif et al. 2020; Kulkarni et al. 2020 ).

Stilbene derivatives displayed antiviral activities against SARS-CoV ). The stilbenoid resveratrol (12) exists widely in different plants including grape Vitis vinifera L., Polygonum cuspidatum, and cranberry Vaccinium macrocarpon Aiton (Lin et al. 2017) . Resveratrol inhibits various viral infections, including the inhibition of HIV and SARS-CoV replication . Compound 12 was a potent in vitro inhibitor of SARS-CoV (at ≤ 0.5 mg/ml) and MERS-CoV, at ≤ 62.5 μM within 24 h of infection Lin et al. 2017) . It was predicted that resveratrol (12) could be a potential agent against new HCoVs in the near future (Lin et al. 2017 ).

Polysaccharides isolated from TCM herb Astragalus mongholicus Bunge have been widely used to boost immunity against viruses. A study by Zhang et al. (2018) showed that supplementation with A. mongholicus polysaccharides can inhibit the replication of avian coronaviruses. Extracts of Acanthopanax gracilistylus var. trifoliolatus C.B. Shang, the root of Sophora flavescens Aiton, and the dried root of Sanguisorba officinalis and Torilis elata Spreng. reduced the in vitro replication of SARS-CoV more potently than the ribavirin drug control (Kim et al. 2010) .

Cimicifuga racemosa (L.) Nutt., Melia azedarach L., Coptis chinensis Franch., Phellodendron chinense C.K.Schneid., and Sophora tonkinensis var. polyphylla X.C.Huang & Z.C.Zhou decreased mouse hepatitis virus A59 (MHV-A59) production and intracellular viral RNA and protein expression with EC 50 values ranging from 2 to 27.5 μg/ml (Kim et al. 2008) . Cimicifuga racemosa, M. azedarach, C. chinensis, P. chinense, and S. tonkinensis were good candidates for the treatment of coronaviral infections in both humans and animals (Kim et al. 2008 ). The underlying antiviral mechanism was most likely due to the inhibition of RNA-dependent RNA polymerase or proteases that are crucial for coronavirus RNA replication. Melia azedarach and C. chinensis may also affect virus assembly or release.

Silvestrol (13), a natural cyclopenta[b]benzofuran, isolated from the fruits and twigs of Aglaia foveolata Pannell, at 0.6-2 μM, inhibited cap-dependent viral mRNA translation of HCoV-229E with an IC 50 of 40 nM (Müller et al. 2018) .

Ouabain (14), also known as g-strophanthin, is a toxic cardiac glycoside that was traditionally used as an arrow poison for both hunting and warfare in eastern Africa. However, this substance, in lower doses, can be used medically to treat hypotension and some arrhythmias. In addition, ouabain diminishes SARS-CoV titers and the number of SARS-CoV RNA copies at 0-3000 nM (Yang et al. 2018) . Wu et al. (2020) described drug names, chemical structures, pharmacological functions, and sources of 20 potential RdRp inhibitors from an in-house natural product database. Their results were based on computer virtual screening because no in vitro and in vivo experiments were conducted.

Andrographolide has been studied for its effects on cell signaling, immunomodulation, and stroke. Using computational approaches, Enmozhi et al. (2020) showed that andrographolide (15), an extremelly bitter labdane diterpenoid isolated from Andrographis paniculata (Burm.f.) Wall. ex Nees, docked successfully in the binding site of SARS-CoV-2 M pro . The molecule obeys Lipinski's rule making it a promising compound to treat COVID-19 (Enmozhi et al. 2020) .

Omacetaxine mepesuccinate (trade names Synribo), formerly named as homoharringtonine (16), is a plant alkaloid derived from Cephalotaxus fortunei Hook that was approved by the US FDA in October 2012 for the treatment of chronic myeloid leukemia in adults. Homoharringtonine has been reported to exhibit potent inhibitory activity against coronaviruses, namely porcine epidemic diarrhea virus, murine hepatitis virus (Dong et al. 2018; Andersen et al. 2019) , and SARS-CoV-2 with EC 50 at 2.10 μM (Choy et al. 2020 ).

Tylophorine (17), a phenanthraindolizidine alkaloid, has anti-HCoV activity in the nanomolar concentrations, up to 1000 nM . Tylophorine derivatives, including naturally occurring and synthetic phenanthroindolizidines and phenanthroquinolizidines, have been identified as potent in vitro inhibitors of enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV). This class of potent compounds showed EC 50 values ranging from 8 to 1468 nM as determined by immunofluorescent assay of the expression of TGEV spike and nucleocapsid proteins and by real-time qRT-PCR analysis of viral yields. Tylophorine compounds could be novel leads for further drug development on their own or as templates for drug design . Tylophorine and related alkaloids, isolated from Tylophora indica Merr., inhibit TGEV replication for anti-coronavirus activity and suppress nitric oxide production in RAW264.7 cells, a measure of antiinflammation (Lee et al., 2012a, b) . Additionally, a pharmacokinetic study demonstrated high and comparable oral bioavailabilities of tylophorine (65.7%) in rats (Lee et al., 2012a, b) .

The chymotrypsin-like protease (3CL pro ) is vital for SARs-CoV replication and is therefore a promising drug target. The active site of SARS-CoV 3CL pro has a catalytic dyad with the sulfur of Cys145 as a nucleophile and the imidazole ring of His41 as a general base (Ryu et al. 2010a) . Also called main protease (M pro ), the 3CL pro of SARS-CoV mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins; thus, it is an important target for drug development . Curcumin inhibits SARS-CoV 3CL pro (IC 50 value of 23.5 μM) (Ryu et al. 2010a ). The extracts of Cibotium barometz (L.) J.Sm. and Dioscorea batatas Decne. also inhibited SARS-CoV 3CL pro activity with IC 50 values of 39 μg/ml and 44 μg/ml, respectively (Cuong et al. 2009; Wen et al. 2011) . Tannic acid, 3-isotheaflavin-3-gallate, and theaflavin-3,3′digallate, three phenolic compounds from black tea, inhibit SARS-CoV 3CL pro with IC 50 values of 3, 7, and 9.5 μM, respectively .

Isatis indigotica Fortune root extracts were frequently used as a remedy during the 2002/2003 SARS outbreak in China. A water extract of I. indigotica had anti-SARS-CoV 3CL pro activity . The root extract of I. indigotica contains indigo, indirubin, indican (indoxyl-β-D-glucoside), β-sitosterol, γ-sitosterol, and sinigrin (18). The IC 50 in the cell-free assays was 115 μM for β-sitosterol, 121 μM for sinigrin, and 300 μM for indigo. The cell-based assay indicated that the antioxidant sinigrin (IC 50 217 μM) was more efficient in blocking the cleavage processing of the 3CL pro than indigo (IC 50 752 μM) and β-sitosterol (IC 50 1210 μM) . Compound 18 is a glucosinolatetype compound also found in some plants of the family Brassicaceae such a Brussels sprouts, broccoli, and the seeds of black mustard (Brassica nigra (L.) Andrz.

Echinaforce® is a standardized preparation extracted from freshly harvested herb (herba tintura 2580 mg/125 drops) and roots (radix tintura 135 mg/125 drops) of Echinacea purpurea (L.) Moench plants (purple coneflower) with a 65% alcoholic solution (Schapowal 2020) . Echinaforce® reduces the infectivity of HCoV-229E in a dose-dependent manner. It inhibits HCoV-229E infection of respiratory epithelial cells (Schapowal 2020) . HCoV-229E was irreversibly inactivated when exposed to Echinaforce® (IC 50 of 3.2 μg/ml) (Schapowal 2020) . Echinaforce® showed a dosedependent reduction of HCoV 229E infectivity with an IC 50 = 9 ± 3 μg/ml (Engler et al. 2017) . Complete neutralization was achieved with 50 μg/ml (Engler et al. 2017) .

Similar inhibition was observed for MERS-CoV when 10 μg/ml of Echinaforce® reduced infectivity by 99.9% and 50 μg/ml of Echinaforce fully blocked infectivity (Engler et al. 2017 ). Extracts of Echinacea purpurea have been used traditionally in North America for the treatment of various infections and wounds, and they have become very popular herbal medicines globally (Hudson 2012) . Rondanelli et al. (2018) found that a combination of E. purpurea, vitamin D, vitamin C, and zinc was useful in the treatment of the common cold. Vitamin D supplementation also lessens the risk of COVID-19 infection and deaths (Grant et al. 2020) . Echinacea angustifolia DC., Echinacea pallida (Nutt.) Nutt., and Echinacea purpurea are the most common Echinacea Moench species recognized for treating common cold and SARS (Hudson 2012) .

Coronavirus infection in humans is characterized by uncontrolled replication of the virus and a prominent proinflammatory response (Wong and Yuen 2008) . Still, in the management of coronavirus disease, the role of immunomodulators to decrease excessive inflammation remains elusive (Wong and Yuen 2008) . The immunomodulatory compounds tomentins A-E (19-23), geranylated flavonoids, isolated from Paulownia tomentosa (Thunb.) Steud. lower the concentration of pro-inflammatory cytokines interleukin-1β and tumor necrosis factor alpha . These tomentins could be useful in allaying the multisystem inflammatory syndrome, also known as the cytokine storm, seen in many COVID-19 patients.

A n t i -i n f l a m m a t o r y a n d a n t i n e o p l a s t i c bisbenzylisoquinoline alkaloids isolated from Stephania tetrandra S.Moore, namely cepharanthine (24), fangchinoline, and tetrandrine, acted as immunomodulators and inhibited the expression of HCoV-OC43 spike and nucleocapsid proteins (Kim et al. 2019) . After the cells were infected with HCoV-OC43, each compound (5 μM) was added to the wells, and cytokine mRNA was qantified by real-time qRT-PCR. Compound treatment following virus infection reduced the mRNA expression levels of IFN-α1 by almost 20-fold at 4 days post infection, and about 100-fold reduction for IFN-β1 at 3 days post infection (Kim et al. 2019 ). According to Islam et al. (2020) , natural products with anticoronavirus activity are major constituents of common dietary supplements and can therefore be used to improve the immunity of the general population in pandemics such as COVID-19.

According to Kwon et al. (2013) , five phlorotannins of the edible brown algae Ecklonia cava Kjellman competitively inhibit the binding of SARS-CoV spike protein to sialic acids at a concentration of less than 36.6 μM. Two phlorotannins caused the inhibition of SARS-CoV RNA and SARS-CoV protein synthesis in late stages, with IC 50 values of 12.2 ± 2.8 and 14.6 ± 1.3 μM, respectively . The results suggest that compounds isolated from E. cava have strong antiviral activity and may be developed into natural therapeutic drugs against SARS-CoV infection . A commercially available E. cava extract, Seapolynol™, was approved as a new dietary ingredient (NDI) by the US Food and Drug Administration in 2008 (FDA-1995-S-0039-0176) (Lee et al., 2012a, b) .

An ethanol extract of the edible brown algae Ecklonia cava, Laminariaceae, contained eight phlorotannins that exhibited a dose-dependent (competitive) inhibitory effect on SARS-CoV 3CL pro with IC 50 values ranging from 2.7 to 164.7 μM . Dieckol showed the most potent SARS-CoV 3CL pro trans/cis-cleavage inhibitory effects. Isolated from the marine alga Halimeda tuna (J.Ellis & Solander) J.V.Lamouroux, halitunal is a novel diterpene aldehyde with a unique cyclopentadieno[c]pyran ring system. Halitunal displayed antiviral activity against murine coronavirus A59 in vitro (Gustafson et al. 2004) . Dercitin, an acridine alkaloid isolated from a marine sponge in the genus Dercitus Gray, inhibited the coronavirus A59 strain (EC 50 1.8 μM).

Mycalamides A and B, protein synthesis inhibitors and antiviral compounds isolated from a sponge Mycale incrustans Burton, also inhibited coronavirus A59 (Laport et al. 2009 ). In vivo activity against coronavirus A59 was observed in mice treated with a 2% mycalamide mixture at a dosage of 0.2 μg/kg daily with 100% survival over a 2-week period (Gallimore 2017) . Triterpenes (thyrsiferol, thyrsiferol acetate, venustatriol) isolated from the red alga Laurencia venusta Yamada inhibit coronaviruses, and orthosterol disodium sulfates from the sponge Petrosia weinbergi Van Soest inhibit coronavirus A59 (El Sayed 2000) .

Spirulina platensis (Gomont) Geitler is a microscopic filamentous alga rich in proteins, vitamins, essential amino acids, minerals, and essential fatty acids like γ-linolenic acid. It is produced commercially and sold as a food supplement in health food stores around the world. Spirulina platensis (15 g) enhances the immune status and inflammatory and oxidative markers of COVID-19 patients (McCarty and DiNicolantonio 2020).

China Xiao et al. (2003) provided leading insights into the use of fourteen Chinese herbal medicines in the prevention and treatment of SARS. TCM played an important role in the fight against SARS and it was reported by practitioners to be very effective (Lau et al. 2005) . The State Administration of TCM of the People's Republic of China formed a panel of TCM specialists to draw up a technical scheme for the prevention and treatment of SARS-CoV using TCM . Scutellaria baicalensis is one of the most widely used TCMs and its roots are used to treat inflammation, cancer, and viral and bacterial infections. Baicalin, baicalein, wogonin, and oroxylin A are the main active components in S. baicalensis. The most abundant active compound, baicalin, exhibits anti-SARS-CoV properties. Yeh et al. (2013) affirmed that licorice (Glycyrrhiza uralensis Fisch. ex DC.) is a common ingredient in prescriptions of TCM. Licorice has antiviral activity against various DNA and RNA viruses including HIV and SARS-CoV. Chinese multi-plant remedy consisting of Houttuynia cordata Thunb., Chrysanthemum morifolium Ramat., Artemisia scoparia Waldst. & Kit., Eupatorium fortunei Turcz., and Amomum tsao-ko Crevost & Lemarié was effective in the prevention and treatment of SARS-CoV (Zhang and Chen 2008) . Extracts of Lonicera japonica are a common TCM used to treat SARS-CoV (Wang et al. 2014) . To control SARS-CoV in 2003, L. japonica was widely used in prescriptions publicly published by the State Administration of TCM.

Panax ginseng C.A.Mey., commonly known as ginseng, is a known natural antiviral agent with protective effects against SARS-CoV (Im et al. 2016) . Extracts of T. sinensis inhibit SARS-CoV replication Wu et al. 2014 ). Extracts of plants Lycoris radiata Miq., Artemisia annua L., Pyrrosia lingua (Thunb.) Farw., and Lindera aggregata (Sims) Kosterm. displayed anti-SARS-CoV actions with EC 50 values of 2.4 ± 0.2, 34.5 ± 2.6, 43.2 ± 14.1, and 88.2 ± 7.7 μg/ml, respectively (Li et al. 2005 ). An ethanol stem cortex extract of L. radiata was very potent against SARS-CoV (EC 50 of 2.1 ± 0.2 nm against the BJ-006 viral strain) (Li et al. 2005) . The active compound lycorine was identified as the anti-SARS-CoV component (EC 50 value of 15.7 ± 1.2 nM). Lycorine shows moderate to potent antiviral activity and reduces viral titers of SARS-CoV (Roy et al. 2018) . Jinchai is a widely used TCM that deters SARS-CoV entry, replication, and lung inflammation induced by viral infection (Zhong et al. 2013) .

Roots and rhizomes of Glycyrrhiza glabra, a natural sweetener that contains glycyrrhizin, inhibit virus growth and inactivate virus particles. Glycyrrhizic acid, a major triterpene glycoside isolated from Glycyrrhiza species, has activity against several enveloped viruses including SARS-CoV and HIV . Diammonium glycyrrhizinate, extracted and purified from G. uralensis, inhibited SARS-CoVs . Ho et al. (2007) found that two widely used TCM plants in the family Polygonaceae inhibited the interaction of SARS-CoV spike protein and ACE2; the IC 50 values for extracts of the root tubers of Rheum officinale Baill. and the root tubers/vines of Polygonum multiflorum ranged from 1 to 10 μ/ml (Ho et al. 2007 ). Chen et al. (2020) stated that the mortality of COVID-19 patients in China was markedly reduced because of effective combination therapy of TCM and Western medicines. At least 20 patent TCMs were recommended by government guidelines in the treatment of COVID-19 patients in China . Several TCM plant formulae for treating SARS-CoV and COVID-19 were reported by Yang et al. (2020) .

In Lebanon, the oil of Laurus nobilis Cav. exerted remarkable activity against SARS-CoV with an IC 50 value of 120 μg/ml and a selectivity index of 4.16 (Loizzo et al. 2008 ). An ornamental tree and a member of the Cupressaceae family, Cupressus sempervirens L. contains flavonoid derivatives, dit e r p e n e s , c a t e c h i n s a n d f l a v o n o l i c o l i g o m e r s , proanthocyanidins, phenolic acids, fatty acids, and essential oils (Orhan and Tumen 2015) . The essential oil of C. sempervirens had a mild inhibitory outcome on SARS-CoV activity (700 ± 2.3 μg/ml) (Orhan and Tumen 2015; Loizzo et al. 2008) . Volatile oils are potential agents for treatment of SARS-CoV (Pasdaran et al. 2016 ). An ethanolic extract of Thymus vulgaris Sm. found in Lebanon inhibits SARS-CoV (Bekut et al. 2018) . Lebanese essential oils have also been evaluated for their inhibitory activity against SARS-CoV replication in vitro by visually scoring the virus-induced cytopathogenic effect post infection.

The papain-like protease (PL pro ) encoded by SARS-CoV cleaves the viral replicase polyprotein. Since this enzymatic activity is important for viral infection, PL pro is a target of interest in the development of antiviral therapies. Nine diarylheptanoids from the bark of Alnus japonica (Thunb.) Steud. were evaluated for their inhibitory activities against SARS-CoV PL pro . Hirsutenone was found to be the most potent inhibitor of SARS-CoV PL pro (IC 50 of 4.1 ± 0.3 μM).

Seven tanshinones isolated from Salvia miltiorrhiza Bunge. were selective inhibitors of SARS-CoV 3CL Pro and PL Pro viral cysteine proteases with IC 50 values from 0.8 to 30 μM (Park et al. 2012b) . Cryptotanshinone was the most potent inhibitor of SARS-CoV PL Pro . Cho et al. (2013) obtained 12 flavonoids from the fruits of Paulownia tomentosa that function as SARS-CoV PL Pro inhibitors. These flavonoids resulted in significant inhibition of SARS-CoV PL pro in a dose-dependent manner. Tomentin E exhibited the highest inhibitory effect with an IC 50 of 5 ± 0.06 μM . Tomentin isolated from Sphaeralcea angustifolia (Cav.) G.Don inhibited PL Pro with IC 50 values ranging from 5 to 14.4 μM.

Singapore Chiow et al. (2016) conducted in vitro virus neutralization assays where an ethyl acetate (EA) fraction of Houttuynia cordata and three of its constituent flavonoids were tested on murine coronavirus. With no cytotoxicity, the EA fraction of H. cordata, added before the viral adsorption stage, inhibited murine coronavirus (IC 50 0.98 μg/ml) (Chiow et al. 2016) . The flavonoid quercetin weakly inhibited murine coronavirus with a minimum inhibitory concentration of 125 μg/ml, cytotoxicity CC 50 value of 116.50 μg/ml, and selectivity index of 0.93. The EA fraction and quercetin yielded a long-lasting anti-murine coronavirus effect of up to 6 days. Overall, this study demonstrated that the EA fraction of H. cordata and its flavonoid component, quercetin, neutralizes murine coronavirus in vitro (Chiow et al. 2016 ).

The EPs® 7630 liquid herbal drug Umckaloabo®, a 100 μg/ml extract of Pelargonium sidoides DC., exerts antiinfluenza virus activity in vitro and in vivo (Theisen and Muller 2012) . Discovered in South Africa, Umckaloabo, a decoction of the roots of Pelargonium sidoides, was originally used by Zulu traditional healers as a remedy for acute bronchitis. Umckaloabo® is known to inhibit the entry and replication of HCoV-229E (Michaelis et al. 2011) . It is a common medication for acute bronchitis in South Africa and Germany.

Although uncontrolled human trials for SARS-CoV anti-infectives have been reported, no randomized controlled trials with a specific anti-coronavirus agent had been conducted with respect to therapy or prophylaxis (Wong and Yuen 2008) . There had not been any clinical trials for drugs of infections caused by HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1 (Wong and Yuen, 2008) . urged that randomized placebo control trials should assess the efficacy of intravenous baicalin (10) for the treatment of SARS especially in developing countries where such formulations were available and affordable. In a randomized double-blinded placebo controlled trial, quercetin supplementation in doses of 500 and 1000 mg/day for 12 weeks significantly increased plasma quercetin levels with no reported side effects, but neither decreased total number of upper respiratory tract infection sick days nor reduced symptomatology in all subjects (Heinz et al. 2010) . Liu et al. (2012) reviewed 12 randomized controlled trials and one quasirandomized controlled trial where 12 herbal remedies were administered on 640 SARS patients.

Case reports, case series, controlled observational studies, and randomized clinical trials provided compelling data that TCM had beneficial effects in the treatment or prevention of SARS (Lau et al. 2005) . For example, the rate of fatality in Hong Kong and Singapore was approximately 18%, while the rate for Beijing was initially more than 52% until the 5th May 2003 when it decreased to 4%, and then to 1% after 20th May 2003 . The dramatic reduction in fatality in Beijing was associated with the use of TCM as a supplement to conventional therapy (Chen and Nakamura 2004) . Lau et al. (2005) reported that during the SARS outbreak of 2002/2003, 1063 volunteers including 926 hospital workers and 37 laboratory technicians working in high-risk virus laboratories used TCM, namely Sang Ju Yin plus Yu Ping Feng San. Compared with the 0.4% of infection in the placebo group, none of the TCM users was infected. In a controlled clinical study, Hsu et al. (2006b) reported that supplementary treatment with TCM resulted in marked relief of symptoms and truncated the disease course. The clinical benefits of TCM were supported by laboratory studies especially for the use of glycyrrhizin (5), baicalin (10), and MOL376, an inhibitor of cathepsin L that could become a lead compound for SARS therapy .

After a review of eight randomized controlled trials, Liu et al. (2004) resolved that a combination of TCM with conventional medicine had beneficial effects such as decrease of mortality, relief of symptoms, and control of fungal infections in patients with SARS. However, the trial evidence was not sufficient enough due to the lack of methodological rigour. In a review of 90 peer-reviewed Chinese publications after the SARS epidemic of 2002 , Leung (2007 concluded that TCM used together with conventional treatment had positive effects including better control of fever and quicker amelioration of chest infection. Wu et al. (2008) urged a rerun of clinical trials involving TCM for the treatment of SARS because the validity of results was jeopardized by questionable trial designs and experimental biases. TCM should be evaluated in carefully designed clinical trials, either used alone or integrated with Western medicine, to cover the prevention and treatment of patients suffering from COVID-19 pneumonia (Ling 2020) . By mid-March 2020, at least 14 TCM clinical trials with a total of 2714 patients were ongoing for the treatment of SARS-CoV-2 infection . Chinese treatment data showed that patented TCM had good therapeutic efficacy in the treatment of COVID-19, with minimal adverse reactions (Zhuang et al. 2020 ).

Empirical data on SARS-CoV treatments are scarce (Wong and Yuen 2008) , and the majority of viral diseases do not have targeted drugs or vaccines (Mahapatra et al. 2019) . Although the ongoing SARS-CoV-2 global pandemic should remind scientists that current options for treating life-threatening zoonotic coronavirus infections are very limited , medicinal plants offer a strong pipeline for the discovery of novel lead compounds that can be converted into new drugs to treat COVID-19. Medicinal plants hold great promise for drug development against SARS-CoV-2, but there is paucity of research on the development of anti-SARS-CoV-2 drugs from natural products. In addition, most of the data on natural products with activity against HCoVs are from non-clinical and pre-clinical studies .

Potential anti-coronavirus therapies can be divided into two categories depending on their target: those that act on the human immune system or human cells, and those that interfere with the coronavirus itself . The unmet need for pharmacologically potent and safe antivirals ought to be fulfilled by thinking out of the box and indigenous knowledge of medicinal plants may be a masterstroke against deadly infectious diseases such as COVID-19 (Mahapatra et al. 2019) . Vellingiri et al. (2020) postulated that medicinal plants are potential sources of drugs for the treatment of COVID-19. Ling (2020) also submitted that traditional plant medicines are a good source of natural compounds for the discovery and development of drugs against SARS-CoV-2. So, the mortality of COVID-19 patients in China was limited by the use of TCM. As the growing COVID-19 global pandemic reformats the classical norms of pharmaceutical and clinical interventions, this review is a poignant reminder that repurposing of current medicinal plants and other natural products to treat COVID-19 should become part of our new vernacular health system.

Comparative genomics lends credence to the repurposing of current anti-SARS natural products for the treatment of COVID-19. This is because the genome sequence of SARS-CoV is very similar to that of SARS-CoV-2 (Tahir ul Qamar et al. 2020) . Phylogenetic analysis of whole genomes reveals that SARS-CoV-2 shares 79.7% nucleotide sequence identity with SARS-CoV Zhou et al., 2020a, b) . Compared with SARS-CoV, the envelope and nucleocapsid proteins of SARS-CoV-2 share sequence identities of 96% and 89.6%, respectively (Zhou et al., 2020a, b) . The virion of SARS-CoV-2 consists of a similar structure as SARS-CoV and MERS-CoV. Like SARS-CoV, SARS-CoV-2 also uses ACE2 as its cellular receptor to enter host cells (Zhou et al., 2020a, b) . The chymotrypsin-like protease (3CL pro ) of SARS-CoV is conserved and shares 99.02% sequence identity with SARS-CoV-2 3CL pro (Tahir ul Qamar et al. 2020 ). These comparative genomic similarities signify one epic biomedical prospect: if medicinal plants used against SARS-CoV and MERS-CoV are carefully repurposed, they could be effective against SARS-CoV-2. In this new normality, natural products should become part of the COVID-19 survival kit.

In the absence of an effective and safe drug that inhibits SARS-CoV-2, the use of antimalarial plants and synthetic drugs has gained traction in the treatment of COVID-19. The notion that antimalarial plants in the genus Artemisia L. can be repurposed to prevent and treat COVID-19 has often been associated with experimental data that chloroquine inhibits the replication of viruses. Chloroquine inhibits chikungunya virus replication in Vero A cells (IC 50 7 μM, IC 90 15 μM, selectivity index = 37.14) in a dose-dependent manner. Chloroquine, whose chemical structure mimics nucleoside analogues, has in vitro activity against SARS-CoV and SARS-CoV-2 ). An antimalarial drug known since 1934, chloroquine blocks HIV-1 and HCoVs at a very early stage; it also inhibits the replication of MERS-CoV in a dose-dependent mode (EC 50 of 3 μM) (de Wilde et al. 2014 ). Chloroquine exerts antiviral as well as immunomodulatory properties. Chloroquine (EC 50 of 1.13 μM against SARS-CoV-2) blocks viral infection by increasing the endosomal pH required for viral fusion (Vellingiri et al. 2020) . A 9-aminoquinoline, chloroquine inhibits SARS-CoV replication and downregulates IFN-γ and TNF-α production in mice (Prinsloo et al. 2018 ). In the first half of 2020, clinical t r i a l s ( N C T 0 4 2 6 1 5 1 7 a n d N C T 0 4 3 0 7 6 9 3 ) o f hydroxychloroquine treatment for COVID-19 were underway in China (Wang et al. 2020b ). The World Health Organization announced it would resume its halted randomized controlled trials to find out whether hydroxychloroquine was able to prevent COVID-19. This was after the Lancet on 4 June 2020, due to concerns with respect to the veracity of the data and analyses, retracted an influential article which had earlier reported that hydroxychloroquine increased the mortality of COVID-19 patients. On 15 June 2020, the US Food and Drug Administration revoked the emergence use of hydroxychloroquine to treat COVID-19 patients after reports that the drug increases the risk of cardiovascular side effects including cardiomyopathy and cardiac arrest.

Taken together, these developments signify that many countries should carefully evaluate the efficacy and safety of natural antimalarial agents for the possible treatment of COVID-19. Rich in flavonoids such as acacetin, genkwanin, and 7-methoxyacacetin, leaf extracts of Artemisia afra Jacq., a common antimalarial remedy in Kenya, Rwanda, and Zambia (Chinsembu 2015) , should be carefully evaluated in COVID-19 patients. The need for medicinal plants and other natural products to treat COVID-19 patients is urgent especially in Africa where health systems grapple with high caseloads of HIV/AIDS and malaria in addition to being heavily underresourced in terms of funding and staff. Overall, plants with antiplasmodial (Chinsembu 2015) , anti-tuberculosis (Chinsembu 2016b) , and anti-HIV replication (Chinsembu 2019 ) activities may present good prospects for the discovery of new active compounds with anti-SARS-CoV-2 action. Pechuel-loeschea leubnitziae O. Hoffm. (Asteraceae), an indigenous plant used to "steam" patients suffering from pneumonia and coughs in Namibia Mofolo et al. 2020) , should also be evaluated for anti-COVID-19 function. High-throughput screening of these plants should be conducted, and computational techniques can quicken the process.

Moreover, plant active compounds should be subjected to absorption, distribution, metabolism, and excretion (ADME) evaluation to verify that oral administration would be effective ). Analysis of structure-activity relationships may be helpful in understanding the mechanisms of certain active compounds such as myricetin (5), baicalein (6), and quercetin. Further studies including in silico molecular docking simulations should be done to investigate the binding affinities of plant active compounds to SARS-CoV-2. A docking analysis can test whether the active compound has the potential to directly interact with SARS-CoV-2 proteins ). Application of structure-based drug design strategies may aid in the development of novel SARS-CoV-2 inhibitors from natural compounds. To predict the general in vivo effects of the natural compounds, network pharmacology analysis can be carried out.

Only 0.0004% plant samples may directly lead to a commercial drug (Chinsembu 2016a) . Thus, there are few plantderived anti-SARS agents in clinical trials. From 2012 to 2017, only twelve new antivirals were approved by the US Food and Drug Administration; eight were for the treatment of pathologies related to hepatitis C virus and two were combinations of anti-HIV drugs (Mercorelli et al. 2018) . Since less than 15% of plant diversity has been explored for pharmaceutical purposes (Chinsembu, 2016a) , the transition from natural product to anti-SARS-CoV-2 drug prototypes is a daunting reality as much as it is a beautiful prospect.

This review shows that plants, fungi, and marine organisms have anti-SARS-CoV properties. Many of these plants, fungi, and marine organisms have other pharmacological benefits because structural diversity and adaptation to various environmental conditions induce them to synthesize defensive compounds with assorted biological activities (Prinsloo et al. 2018) . Corollary, diverse plant secondary metabolites are an important library of novel lead compounds that may be functional against SARS-CoV-2. Bio-assay-guided fractionation of medicinal plant extracts may provide a quicker pipeline for the discovery of novel compounds against SARS-CoV-2.

An examination of the research publications on anti-SARS natural products cited in this review shows that about 8 out of 10 are authored by the Chinese. Since the Chinese represent about 80% of all research efforts and publications on anti-SARS natural products, there is 80% chance that the next big discovery of novel natural products to treat COVID-19 will be from TCM. China is the world leader in research and development for innovative anti-SARS agents from natural products. By all intents and purposes, the first wellresearched and clinically proven herbal remedy for COVID-19 will most likely be discovered and developed in China.

The most notable gap identified in the current literature is that many countries in Africa, Asia (except China), Australia/ Oceania, Europe, North America, and South America lag behind in terms of research efforts on anti-coronavirus remedies from medicinal plants and other natural products. In many countries all over the world, there is need to conduct detailed ethnobotanical studies to establish putative anti-SARS-CoV-2 medicinal plants and their active compounds. The search for new anti-SARS-CoV-2 agents should be rigorously extended to marine organisms. This review shows that there is a dearth of experimental data on posology and cytotoxicity of plant extracts and active compounds with in vitro activity against SARS-CoV-2. More studies on pan-assay interference compounds (PAINS) and SARS-CoV-2 immunomodulatory agents from plants and other natural products should be conducted. There is also lack of pharmacological and rigorous human clinical trial data in relation to natural products for treating COVID-19.

The world is in the middle of a COVID-19 pandemic. Although the deadly threat posed by the current COVID-19 pandemic requires the development of new therapeutic agents, there are currently no efficacious and safe vaccines or drugs to prevent or treat this highly infectious disease. Even in developed nations, blockbuster vaccines or drugs have not yet been developed or approved for the prevention and treatment of COVID-19. Only one synthetic drug (remdesivir) has been approved for emergency use in the USA. Compared with placebo, remdesivir did not significantly reduce the mortality of COVID-19 patients in clinical trials. Against this backdrop, this review provides a modest but insightful snapshot of medicinal plants, fungi, and marine organisms that contain putative active compounds that inhibit SARS-CoV-2. Data in this review show two recurring motifs. The first one is that the search for herbal remedies against COVID-19 is more intense in China than in any other part of the world. The second is that most putative anti-SARS-CoV-2 remedies contain active compounds that inhibit HCoVs at the cellular and molecular levels. Since structure dictates function, many of these compounds inhibit SARS-CoV replication because their chemical structures mimic nucleoside analogues. While many of the natural products possess anti-SARS-CoV properties, their extracts and active compounds should be evaluated for human cytotoxicity and dosage. Putative anti-SARS-CoV-2 extracts and active compounds should be rigorously tested in animal and randomized placebo clinical trials.

Due to the current lack of effective and safe drugs, natural products presented in this review may form part of the health beliefs, self-medication choices, and selfefficacy practices that people may use to manage COVID-19. Natural products presented in this paper form a repertoire of promising leads that may be repurposed into novel drugs, nutraceuticals, and supplements for COVID-19. If there is a long-term remedy for COVID-19, it must lie in Mother Nature's pharmacy of SARS-CoV-2 inhibitors and immunomodulators from plants, fungi, and marine organisms.

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Using herbal medicine as a means of prevention experience during the SARS crisis

Griffithsin, a highly potent broad-spectrum antiviral lectin from red algae: from discovery to clinical application

Effects of Ecklonia cava polyphenol in individuals with hypercholesterolemia: a pilot study

Synthesis and biological evaluation of tylophorinederived dibenzoquinolines as orally active agents: exploration of the role of tylophorine e ring on biological activity

The efficacy of Chinese medicine for SARS: a review of Chinese publications after the crisis

Synthesis of stilbene derivatives with inhibition of SARS coronavirus replication

Coronavirus infections and immune responses

Identification of natural compounds with antiviral activities against SARS-associated coronavirus

Extracts of the medicinal herb Sanguisorba officinalis inhibit the entry of human immunodeficiency virus-1

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Effective inhibition of MERS-CoV infection by resveratrol

Traditional Chinese medicine is a resource for drug discovery against 2019 novel coronavirus (SARS-CoV-2)

Chinese herbal medicine for severe acute respiratory syndrome: a systematic review and metaanalysis

Chinese herbs combined with Western medicine for severe acute respiratory syndrome (SARS)

The roles of direct recognition by animal lectins in antiviral immunity and viral pathogenesis

Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

Ethnomedicinal wisdom: an approach for antiviral drug development

Nutraceuticals have potential for boosting the type 1 interferon response to RNA viruses including influenza and coronavirus

Drug repurposing for viral infectious diseases: how far are we?

Investigation of the influence of EPs® 7630, a herbal drug preparation from Pelargonium sidoides, on replication of a broad panel of respiratory viruses

Antiviral lectins: selective inhibitors of viral entry

Green synthesis of silver nanoparticles from extracts of Pechuelloeschea leubnitziae: their anti-proliferative activity against the U87 cell line

Angiotensin converting enzyme-inhibitory triterpens from Ganoderma lucidum

Broad-spectrum antiviral activity of the eIF4A inhibitor silvestrol against corona-and picornaviruses

Potential of flavonoid-inspired phytomedicines against COVID-19

Potential of Cupressus sempervirens (Mediterranean cypress) in health

Diarylheptanoids from Alnus japonica inhibit papain-like protease of severe acute respiratory syndrome coronavirus

Tanshinones as selective and slow-binding inhibitors for SARS-CoV cysteine proteases

Dieckol, a SARS-CoV 3CL pro inhibitor, isolated from the edible brown algae Ecklonia cava

Volatile oils: potential agents for the treatment of respiratory infections

Recent discovery and development of inhibitors targeting coronaviruses

Coronavirus (COVID-19), first indication of efficacy of gene-eden-VIR/novirin in SARS-CoV-2 infections

Anti-HIV activity of southern African plants: current developments, phytochemistry and future research

Medicinal plants of the genus Betula-traditional uses and a phytochemical-pharmacological review

COVID-19 and Italy: what next?

Self-care for common colds: the pivotal role of vitamin D, vitamin C, zinc, and Echinacea in three main immune interactive clusters (physical barriers, innate and adaptive immunity) involved during an episode of common colds-practical advice on dosages and on the time to take these nutrients/botanicals in order to prevent or treat common colds

Lycorine: a prospective natural lead for anticancer drug discovery

Biflavonoids from Torreya nucifera displaying SARS-CoV 3CL pro inhibition

SARS-CoV 3CL pro inhibitory effects of quinone-methide triterpenes from Tripterygium regelii

Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo

Use of Echinaforce to prevent coronavirus infections

Myricetin: a dietary molecule with diverse biological activities

Triterpenoids manipulate a broad range of virushost fusion via wrapping the HR2 domain prevalent in viral envelopes

Different host cell proteases activate the SARS-coronavirus spikeprotein for cell-cell and virus-cell fusion

Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry

Structural basis of SARS-CoV-2 3CL pro and anti-COVID-19 drug discovery from medicinal plants

EPs® 7630 (Umckaloabo®), an extract from Pelargonium sidoides roots, exerts anti-influenza virus activity in vitro and in vivo

COVID-19: a promising cure for the global panic

Receptor recognition by the novel coronavirus from Wuhan: an analysis based on decadelong structural studies of SARS coronavirus

Glycyrrhizic acid as the antiviral component of Glycyrrhiza uralensis Fisch. against coxsackievirus A16 and enterovirus 71 of hand foot and mouth disease

Discovery of unsymmetrical aromatic disulfides as novel inhibitors of SARS-CoV main protease: chemical synthesis, biological evaluation, molecular docking and 3D-QSAR study

Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro

Subunit vaccines against emerging pathogenic human coronaviruses

A comparative study of Lonicera japonica with related species: morphological characteristics, ITS sequences and active compounds

Specific plant terpenoids and lignoids possess potent antiviral activities against severe acute respiratory syndrome coronavirus

Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication

The management of coronavirus infections with particular reference to SARS

Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods

Chemical composition, antimicrobial activity against Staphylococcus aureus and a pro-apoptotic effect in SGC-7901 of the essential oil from Toona sinensis (A. Juss.) Roem. leaves

Traditional Chinese medicine in the treatment of acute respiratory tract infections

The cardenolide ouabain suppresses coronaviral replication via augmenting a Na+/ K+-ATPase-dependent PI3K_PDK1 axis signaling

Traditional Chinese medicine in the treatment of patients infected with 2019-new coronavirus (SARS-CoV-2): a review and perspective

Water extract of licorice had anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines

Identification of myricetin and scutellarein as novel chemical inhibitors of the SARS coronavirus helicase, nsP13

In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus

Association between mannose-binding lectin gene polymorphisms and susceptibility to severe acute respiratory syndrome coronavirus infection

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Anticomplementary principles of a Chinese multiherb remedy for the treatment and prevention of SARS

Characterization of the inhibitory effect of an extract of Prunella vulgaris on Ebola virus glycoprotein (GP)-mediated virus entry and infection

Complete genomic sequences, a key residue in the spike protein and deletions in nonstructural protein 3b of US strains of the virulent and attenuated coronaviruses, transmissible gastroenteritis virus and porcine respiratory coronavirus

Antiviral activity of Jinchai capsule against influenza virus

Glycopeptide antibiotics potently inhibit cathepsin L in the late endosome/lysosome and block the entry of Ebola virus, Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus (SARS-CoV)

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Inhibitors of SARS-CoV entry-identification using an internally-controlled dual envelope pseudovirion assay

Networkbased drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2

A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms

Chinese patent medicines in the treatment of coronavirus disease 2019 (COVID-19) in China

Procyanidins and butanol extract of cinnamomi cortex inhibit SARS-CoV infection

Acknowledgments The author is grateful to the anonymous reviewers and to Professor Rogelio Pereda-Miranda, Editor-in-Chief of Revista Brasileira de Farmacognosia, for their help in improving the original manuscript.
