key: cord-001765-7wv4cb37
authors: Matassov, Demetrius; Marzi, Andrea; Latham, Terri; Xu, Rong; Ota-Setlik, Ayuko; Feldmann, Friederike; Geisbert, Joan B.; Mire, Chad E.; Hamm, Stefan; Nowak, Becky; Egan, Michael A.; Geisbert, Thomas W.; Eldridge, John H.; Feldmann, Heinz; Clarke, David K.
title: Vaccination With a Highly Attenuated Recombinant Vesicular Stomatitis Virus Vector Protects Against Challenge With a Lethal Dose of Ebola Virus
date: 2015-06-24
journal: Journal of Infectious Diseases
DOI: 10.1093/infdis/jiv316
sha: 
doc_id: 1765
cord_uid: 7wv4cb37

Previously, recombinant vesicular stomatitis virus (rVSV) pseudotypes expressing Ebolavirus glycoproteins (GPs) in place of the VSV G protein demonstrated protection of nonhuman primates from lethal homologous Ebolavirus challenge. Those pseudotype vectors contained no additional attenuating mutations in the rVSV genome. Here we describe rVSV vectors containing a full complement of VSV genes and expressing the Ebola virus (EBOV) GP from an additional transcription unit. These rVSV vectors contain the same combination of attenuating mutations used previously in the clinical development pathway of an rVSV/human immunodeficiency virus type 1 vaccine. One of these rVSV vectors (N4CT1-EBOVGP1), which expresses membrane-anchored EBOV GP from the first position in the genome (GP1), elicited a balanced cellular and humoral GP-specific immune response in mice. Guinea pigs immunized with a single dose of this vector were protected from any signs of disease following lethal EBOV challenge, while control animals died in 7–9 days. Subsequently, N4CT1-EBOVGP1 demonstrated complete, single-dose protection of 2 macaques following lethal EBOV challenge. A single sham-vaccinated macaque died from disease due to EBOV infection. These results demonstrate that highly attenuated rVSV vectors expressing EBOV GP may provide safer alternatives to current EBOV vaccines.

Previously, recombinant vesicular stomatitis virus (rVSV) pseudotypes expressing Ebolavirus glycoproteins (GPs) in place of the VSV G protein demonstrated protection of nonhuman primates from lethal homologous Ebolavirus challenge. Those pseudotype vectors contained no additional attenuating mutations in the rVSV genome. Here we describe rVSV vectors containing a full complement of VSV genes and expressing the Ebola virus (EBOV) GP from an additional transcription unit. These rVSV vectors contain the same combination of attenuating mutations used previously in the clinical development pathway of an rVSV/human immunodeficiency virus type 1 vaccine. One of these rVSV vectors (N4CT1-EBOVGP1), which expresses membrane-anchored EBOV GP from the first position in the genome (GP1), elicited a balanced cellular and humoral GP-specific immune response in mice. Guinea pigs immunized with a single dose of this vector were protected from any signs of disease following lethal EBOV challenge, while control animals died in 7-9 days. Subsequently, N4CT1-EBOVGP1 demonstrated complete, single-dose protection of 2 macaques following lethal EBOV challenge. A single sham-vaccinated macaque died from disease due to EBOV infection. These results demonstrate that highly attenuated rVSV vectors expressing EBOV GP may provide safer alternatives to current EBOV vaccines.

Keywords. attenuation; rVSV vector; Ebola vaccine; glycoprotein; challenge; nonhuman primates.

The genus Ebolavirus is classified within the Filoviridae family and comprises 5 closely related but antigenically distinct virus species: Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus, and Zaire ebolavirus [1, 2] . In nature, all but Reston virus have been isolated only in sub-Saharan Africa, where the likely virus reservoir is thought to be in bats [3, 4] . All but the Tai Forest and Reston viruses have caused sporadic, deadly outbreaks of disease in humans since the discovery of Ebolavirus 38 years ago, with case-fatality rates of 30%-90% [5, 6] . The ongoing 2014 Ebola virus (EBOV) outbreak in West Africa is the largest recorded, with case-fatality rates of around 50%. Hospitalization of patients seemingly improves clinical outcome and public health initiatives may slow the spread of disease, but mass vaccination programs in EBOV-endemic regions will likely be required to extinguish the current outbreak and prevent such occurrences in the future.

The EBOV genome is composed of a single strand of negative-sense RNA, approximately 19 kb in length, encoding 7 major viral polypeptides. One of these proteins is the EBOV glycoprotein (EBOVGP), which mediates virion attachment and fusion to susceptible cells [7] .

The EBOVGP also contains the major virus neutralization epitopes [8, 9] ; accordingly, the EBOVGP has been the target for a variety of EBOV vaccine designs. For a thorough review of vaccine approaches see Falzarano et al [10] . The vector in one such vaccine is recombinant vesicular stomatitis virus (rVSV), in which the gene encoding VSV G has been replaced with the gene encoding EBOVGP [11, 12] . This rVSV pseudotype replicates efficiently in cell culture and also propagates in vivo.

VSV is a member of the Rhabdoviridae family and, similar to EBOV, has a single-strand, negative-sense RNA genome, approximately 11 kb long, encoding 5 major viral proteins. In nature, VSV cycles between biting insects, which are the likely reservoir, and livestock [13] [14] [15] . The virus replicates to high titer in virus-induced vesicles at insect bite sites on the nose, lips, teats, and coronary bands of hooves. Disease in livestock is self-limiting and typically resolves in 10-11 days without significant consequences. VSV can also infect humans who have close contact with infected animals or purified virus [16, 17] . Infection results in mild influenza-like symptoms, which typically resolve in 3-5 days without complications [16, 17] . The very low seroprevalence of VSV in humans favored development of VSV as a vaccine vector and became feasible when Rose et al developed a system for recovery of rVSV from genomic complementary DNA (cDNA) [18] . Since then, rVSV vectors expressing antigens from human pathogens have demonstrated high levels of efficacy in the respective animal disease models [19] [20] [21] [22] .

VSV exhibits a pronounced 3′ to 5′ gradient of gene expression due to discontinuous messenger RNA (mRNA) transcription across intergenic regions [23, 24] , allowing modulation of virus protein or foreign antigen expression as a function of gene distance from the 3′ transcription promoter [25, 26] . A highly attenuated rVSV vector expressing human immunodeficiency virus type 1 (HIV) gag from the first position in the genome was developed and has been demonstrated to be safe in stringent mouse and nonhuman primate (NHP) neurovirulence and biodistribution studies [27] [28] [29] . This rVSV vector was attenuated by combining N gene translocation from position 1-4 (N4) in the genome and truncation of the G protein cytoplasmic tail (CT) from 29 amino acids (aa) in native form to 1 aa (CT1) [28, 30] . This N4CT1-HIVgag1 vector was subsequently shown to be safe and immunogenic in phase 1 clinical trials (HVTN 090 and HVTN 087; available at: http://clinicaltrials. gov/), and after vaccination viremia was undetectable by an VSV infectivity assay or a VSV-specific polymerase chain reaction assay in blood, urine, or saliva specimens obtained from trial participants (unpublished data).

Here we describe the generation of attenuated rVSV vectors expressing EBOVGP and their immunogenicity in mice. The vector that elicited the most balanced EBOVGP-specific humoral and cellular immune response in mice was then evaluated for protective efficacy in guinea pig and NHP challenge studies.

For murine studies, 8-10-week-old female BALB/c mice were used. Mice were maintained according to the Guide for the Care and Use of Laboratory Animals [31] . In addition, procedures for the use and care of the mice were approved by Profectus BioSciences and New York Medical College institutional animal care and use committees (IACUCs). The guinea pig and macaque studies were carried out in strict accordance with the recommendations described in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, the Office of Animal Welfare, and the US Department of Agriculture. All animal work was approved by the National Institute of Allergy and Infectious Diseases Division of Intramural Research IACUC at the Rocky Mountain Laboratories (RML). The facility is accredited by the American Association for Accreditation of Laboratory Animal Care. All procedures were performed on animals after they were anesthetized by trained personnel under the supervision of veterinary staff. All efforts were made to minimize the pain and suffering of animals, with early end point criteria specified by the RML IACUC-approved score parameters used to determine when animals should be humanely euthanized.

As described previously [28, 30] , the N4CT1-HIVgag1 vector was used as template for generating all rVSV/EBOV vectors expressing EBOVGP. The N4CT1-HIVgag1 genomic cDNA was modified by exchanging the gag gene located in position 1 of the genome with the EBOVGP gene (1976, Mayinga isolate), generating N4CT1-EBOVGP1 cDNA (GP1; Figure 1A) . A vector expressing a third-position EBOVGP (GP3; Figure 1A ) was generated by inserting an expression cassette between the M and N genes. To insert the EBOVGP gene in genome position 6 (GP6; Figure 1A ), the N and G genes were swapped and an expression cassette was positioned between VSV L and the trailer sequence. For adventitious agent testing of clinical trial material, large quantities of EBOVGP-specific neutralizing antibody are required to neutralize vaccine virus infectivity. To avoid this requirement, a gene expressing secreted EBOVGP was generated by deleting the sequence encoding the transmembrane region and cytoplasmic tail (aa 651-676). This modified EBOVGP gene was inserted into position 1 of N4CT1 (GP1ΔTM; Figure 1A ). The mucin-like domain of EBOVGP spans aa 305-485, and part of this domain (aa 374-465) was deleted (GP1Δmuc; Figure 1A ). This modified EBOVGP gene was generated following observations that part of the mucin region was deleted upon multiple serial passage of N4CT1-EBOVGP1 on Vero cell monolayers and was included here to assess the effect of partial removal of the glycan "coat" on EBOVGP immunogenicity. EBOVGP expression was analyzed by Western blotting for all vectors ( Figure 1B ).

Vectors were rescued from genomic cDNA as previously described [32] . Rescued virus was plaque purified and amplified on Vero cell monolayers (ATCC CCL-81). For animal studies, vectors were purified from infected cell supernatants by centrifugation through a 10% sucrose cushion. Virus pellets were resuspended in phosphate-buffered saline (PBS), pH 7.0, mixed with stabilizer (7 mM K 2 HPO 4 , 4 mM KH 2 PO 4 , and 218 mM sucrose), snap frozen, and stored at −80°C.

For the mouse immunogenicity study, rVSV/EBOV vectors were administered at 1 × 10 7 plaque-forming units (PFU) by intramuscular injection of the tibialis anterior muscle (total injection volume, 0.05 mL). Serum was collected at weeks 0, 3, and 5. Ten days after final the inoculation, mice were euthanized by CO 2 inhalation, and serum and spleen cells were harvested.

For the mouse neurovirulence study, groups of ten 5-weekold female Swiss Webster mice were anesthetized and inoculated intracranially with 0.02 mL of serial 10-fold dilutions of each vector as previously described [30] . After inoculation, mice were weighed daily and assessed for signs of disease over 3 weeks. Neurovirulence was assessed by measuring morbidity and mortality as an end point. Mice that were showing severe signs of disease or were moribund were promptly euthanized. Cumulative deaths across all doses tested allowed the 50% lethal dose to be calculated for each vector [33] .

For guinea pig immunization, 2 × 10 5 PFU of each vector was administered intraperitoneally. On day 21 after vaccination guinea pigs were challenged with 10 focus-forming units (FFU) of guinea pig-adapted EBOV (GPA-EBOV) [34] by intraperitoneal injection. All animals were weighed daily and monitored for signs of illness.

For macaque challenge, 3 healthy, EBOV-naive, adult (age, 5-10 years; weight, 3-12 kg), male and female Rhesus macaques (Macaca mulatta) were randomly assigned to a vaccination group (n = 2) and a nonvaccination control group (n = 1). At study day −21, macaques were anesthetized with ketamine and inoculated in the caudal thigh muscle with 1 × 10 7 PFU of rVSV/EBOV vector (total volume, 1 mL). At study day 0, macaques were anesthetized with ketamine and challenged with 1000 FFU of EBOV [11] by intramuscular injection of the caudal thigh muscle (total volume, 1 mL). Macaques were monitored for signs of illness (ie, abnormal temperature, weight loss, clinical examination findings, hematology findings, and blood chemistry findings) following vaccination and the EBOV challenge portions of the study.

For murine studies, vaccine-elicited IFN-γ ELISpot responses were determined using a mouse IFN-γ ELISpot kit (BD Biosciences, San Diego, California) as previously described [35] . Splenocytes were incubated with 2 µg/mL Con-A (Sigma), peptide pools (15-mers overlapping by 11 aa; final peptide concentration, 1 µM [each]) spanning EBOVGP, or medium alone.

A murine enzyme-linked immunosorbent assay (ELISA) previously described [35] was modified by using ELISA plates coated with 100 ng/well of purified recombinant EBOVGP (IBT Bioservices) to determine EBOVGP-specific serum immunoglobulin G (IgG) titers. Murine serum samples were added to ELISA plates at a starting dilution of 1:100 and were further diluted 3-fold across the plates. Antigen-specific antibody titers were defined as the reciprocal of the last serum dilution giving an OD 450 of >0.1.

Antigens for the macaque ELISA were produced as previously described [36] . Macaque sera were inactivated by γ-irradiation (5 Mrad) according to a standard operating protocol approved by the institutional biosafety committee at RML. ELISA with EBOVGPΔTM was performed as described previously [36] .

To identify the most-effective rVSV/EBOV vector design for induction of EBOVGP-specific immune responses, the vectors outlined in Figure 1A were compared for their ability to elicit EBOVGP-specific cell-mediated immune and binding antibody responses in mice (Figure 2 ). Groups of BALB/c mice (n = 10) were immunized intramuscularly at study week 0 ( Figure 2A ). Ten days after primary immunizations, splenocytes were collected from 5 mice/group and tested for EBOVGP-specific IFN-γ secretion by ELISpot assay. The remaining 5 mice/ group were boosted intramuscularly at study week 3 with 10 7 PFU of each rVSV/EBOV vector. Ten days after boosting, splenocytes were collected and tested as described above.

After 1 immunization, the highest mean EBOVGP-specific IFN-γ ELISpot responses (±standard error of the mean [SEM]) were detected in mice immunized with N4CT1-EBOVGP1ΔTM (215 ± 25 spot-forming cells [SFCs]/10 6 splenocytes) and N4CT1-EBOVGP1 (133 ± 17 SFC/10 6 splenocytes). Interestingly, N4CT1-EBOVGP1Δmuc, elicited a significantly reduced response (mean ± SEM, 43 ± 19 SFCs/10 6 splenocytes) relative to N4CT1-EBOVGP1 and N4CT1-EBOVGP1. Notably, after 1 immunization, GP responses were undetectable in mice immunized with vectors expressing EBOVGP from genome positions 3 or 6.

After boosting, the highest mean EBOVGP-specific IFN-γ ELISpot responses (±SEM) were again detected in mice immunized with N4CT1-EBOVGP1ΔTM (211 ± 18 SFCs/10 6 splenocytes) and N4CT1-EBOVGP1 (mean ± SEM, 300 ± 57 SFCs/10 6 splenocytes). Mice immunized with N4CT1-EBOVGP1Δmuc showed an improved response (mean ± SEM, 172 ± 41 SFCs/ 10 6 splenocytes), and mice boosted with N4CT1-EBOVGP3 or N4CT1-EBOVGP6 still failed to demonstrate a measurable EBOVGP-specific IFN-γ ELISpot response.

Immunized mice were also monitored for induction of serum antibody responses by ELISA ( Figure 2B ). Ten days after boosting, mean serum EBOVGP-specific IgG titers (±SEM) were highest in mice immunized with N4CT1-EBOVGP1Δmuc vector (5.35 ± 0.08), N4CT1-EBOVGP1 (5.19 ± 0.07), and N4CT1-EBOVGP3 (5.14 ± 0.07). Interestingly, a statistically significant, approximately 10-fold lower EBOVGP-specific IgG titer was detected in mice immunized with N4CT1-EBOVGP1ΔTM (mean ± SEM, 4.05 ± 0.25) relative to mice immunized with N4CT1-EBOVGP1. A 10-fold lower titer was also detected in mice immunized with N4CT1-EBOVGP6 (4.21 ± 0.22) relative to mice immunized with vectors expressing EBOVGP from genome positions 1 or 3.

On the basis of the data presented in Figure 2 , the N4CT1-EBOVGP1 vector expressing an anchored full-length EBOVGP in position 1 of the rVSV genome appeared to be the most immunogenic (with regard to cell-mediated and humoral immunity) in mice, so N4CT1-EBOVGP1 was selected for guinea pig and NHP challenge studies.

We next tested whether N4CT1-EBOVGP1 could protect guinea pigs from a lethal challenge (Figure 3 ). In this experiment, guinea pigs were immunized intraperitoneally with 2 × 10 5 PFU of N4CT1-EBOVGP1. For controls, additional groups of guinea pigs were immunized with 2 × 10 5 PFU of wtVSV or medium alone. On day 21 after immunization, all guinea pigs were challenged intraperitoneally with 10 FFU of GPA-EBOV. After challenge, guinea pigs were monitored for changes in body weight ( Figure 3A) , signs of disease, and survival ( Figure 3B ).

Guinea pigs immunized with medium alone or wtVSV began to lose weight on day 4 after challenge and died from EBOV infection during study days 7 and 9 as observed previously [37] . In contrast, N4CT1-EBOVGP1 immunized guinea pigs gained weight and were completely protected from disease during the course of the study.

Encouraged by findings from the guinea pig study, we determined whether N4CT1-EBOVGP1 could protect macaques from lethal EBOV challenge (Table 1) . Three rhesus macaques were immunized intramuscularly in the caudal thigh muscle as follows: 2 were immunized with 1 × 10 7 PFU N4CT1-EBOVGP1 in a total volume of 1 mL (EBOV1 and EBOV2), and 1 macaque (CTRL) was mock immunized. At day 14 after immunization, both N4CT1-EBOVGP1-immunized macaques demonstrated measurable EBOVGP-specific serum IgG responses (titer, 1:100), and these responses increased (titer, 1:400) by study day 0. In contrast, the sham-immunized macaque remained seronegative for EBOVGP.

At study day 0, all 3 macaques were challenged with 1000 FFU EBOV Mayinga by intramuscular injection in the caudal thigh muscle. As shown in Table 1 , the single control animal failed to mount an EBOVGP-specific response on day 6 after challenge and quickly died from EBOV infection. Importantly, a rapid increase in the EBOVGP-specific serum IgG titer was observed on days 6, 10, and 14 after challenge in both vaccinated macaques, and they survived without showing signs of disease.

Mice are exquisitely sensitive to intracranial instillation of wtVSV [28, 30] , leading to lethal viral encephalitis. Previously, it was shown that intracranial inoculation of 5-week-old Swiss Webster mice with 10 8 PFU of the highly attenuated N4CT1-HIVgag1 vector caused no significant illness, so a 50% lethal dose could not be achieved for this vector [28] . To test the neurovirulence potential of N4CT1-EBOVGP1, groups of outbred Swiss Webster mice were inoculated intracranially with N4CT1-EBOVGP1 in a dose-ranging study (10 4 -10 7 PFU; Figure 4 ). Two control groups were inoculated with 10 2 PFU of a less attenuated rVSV (rVSV-HIVgag5) or with PBS alone. Following inoculation, mice were assessed for morbidity and mortality over 2 weeks.

Consistent with previously published reports [28, 30] , mice inoculated intracranially with PBS survived, and approximately half of the mice inoculated with rVSV-HIVgag5 died 3-5 days after inoculation. Importantly, all mice inoculated with N4CT1-EBOVGP1 survived and were disease free.

Previous work demonstrated that vaccination with a single dose of replication-competent rVSV pseudotyped with EBOVGPs protected mice, hamsters, guinea pigs, and NHPs from lethal EBOV challenge [12, 38, 39] . The rVSV vector used in those studies retained the natural VSV genome organization, except the VSV G gene was replaced with the EBOVGP gene [39] . Here we demonstrate protection of guinea pigs and NHPs with a highly attenuated, replication competent form of rVSV that retains the VSV G gene [27, 28, 30] and expresses the EBOVGP from an additional transcription unit inserted at position 1 in the rVSV genome.

The mouse immunogenicity study demonstrated that EBOVGPspecific cell-mediated immune responses were more robust when the EBOVGP gene was in the first position in the genome, compared with the third and sixth positions, consistent with greater relative abundance of EBOVGP in infected cells due to the steep 3′ to 5′ transcription gradient present during VSV replication [23, 24, 26] . The cell-mediated immune response was similar for secreted and anchored forms of EBOVGP, indicating that both forms of EBOVGP underwent a similar degree of processing and presentation of T-cell epitopes in antigen-presenting cells. Interestingly, there was a significant reduction in the cell-mediated immune response to EBOVGP when part of the mucin-rich region was deleted, possibly as a result of altered proteosomal processing of EBOVGP or direct deletion of T-cell epitopes. After decay of the primary cell-mediated immune response, a second vaccine dose boosted ELISpot responses to levels seen after primary vaccination; however, there was still no detectable cell-mediated immune response elicited by vectors expressing EBOVGP from position 3 and 6, indicating the requirement for higher EBOVGP levels in infected cells to generate detectable EBOVGP-specific cell-mediated immune responses.

As expected, the EBOVGP-specific IgG response in mice was greatest for N4CT1-EBOVGP1 and N4CT1-EBOVGP1Δmuc vectors expressing the highest levels of EBOVGP from genome position 1. Interestingly, N4CT1-EBOVGP1ΔTM elicited much lower levels of EBOVGP-specific IgG than N4CT1-EBOVGP1. We speculate this may reflect differences in the tertiary and quaternary structure of secreted EBOVGP, compared with the trimeric conformation achieved by anchored EBOVGP. Notably, the secreted EBOVGP described here is different from the naturally occurring secreted form of EBOVGP that is translated from unedited EBOVGP mRNA [40] . The corollary is that anchored trimeric EBOVGP spikes on the surface of infected cells and virus particles present a dense array of structural epitopes that may interact more efficiently with B cells than soluble secreted EBOVGP, as is the case for other particulate antigen compositions [41, 42] . EBOVGP-specific IgG responses were highest for N4CT1-EBOVGP1Δmuc [43] , possibly reflecting better exposure of EBOVGP epitopes by removal of part of the carbohydrate cloak [44] . The N4CT1-EBOVGP3 vector also elicited a robust EBOVGP-specific IgG response, which contrasts with the undetectable EBOVGP-specific cell-mediated immune response induced by this vector. The explanation for this anomaly is unclear but could be due to relative differences in EBOVGP-specific cellular and humoral antigenicity. We believe the approximate 30% attenuation of expression across each gene junction could drop the EBOVGP level below a threshold required to elicit cell-mediated immune responses (N4CT1-EBOVGP3), while the EBOVGP level is still adequate to induce a relatively robust humoral response. However, for N4CT1-EBOVGP6, the EBOVGP-specific humoral response does decline markedly, presumably as a result of the calculated 85% reduction in EBOVGP expression when in the sixth position in the genome.

Previous work demonstrated that the N4CT1 vector backbone was highly attenuated in the mouse intracranial NV model [28, 30] . However, Western blot data demonstrated that EBOVGP was present on the surface of virus particles when expressed by an rVSV vector that retained the VSV G gene (unpublished data). Therefore, a mouse NV study was performed to assess any changes in virulence arising from the possible alteration of vector tropism mediated by EBOVGP. Results confirmed the highly attenuated nature of N4CT1-EBOVGP1 in the mouse central nervous system, indicating that the presence of EBOVGP on the virion surface, in addition to VSV G protein, did not enhance virus spread in the brain, presumably because cells in the central nervous system lack the EBOVGPspecific receptor [45] [46] [47] and because virus using VSV G receptors remained attenuated as previously described [30] .

Because N4CT1-EBOVGP1 elicited a desirable balance of humoral and cellular immune responses to EBOVGP after a single vaccine dose in mice, it was selected for guinea pig and NHP challenge studies. One dose of this vector protected guinea pigs from signs of disease following challenge with a lethal dose of GPA-EBOV. Interestingly, there was a slight delay in onset of the first signs of disease after challenge of wtVSV-vaccinated guinea pigs. One possible explanation is that induction of IFNs and other innate immune responses by rVSV [48, 49] leaves the guinea pig innate immune system on high alert, even 21 days after vaccination, resulting in a generalized antiviral state that may initially slow the GPA-EBOV infection [49, 50] .

A single dose of N4CT1-EBOVGP1 vector also protected NHPs from disease following EBOV challenge. An EBOVGPspecific IgG titer was detected in both immunized macaques prior to challenge and was expanded following EBOV challenge, indicating that a preexisting EBOVGP-specific antibody response is important for protection. Unfortunately cell-mediated immune responses in NHPs were not measured and may also contribute significantly to protection. The N4CT1-EBOVGP1 vector used in these challenge studies is similar to the highly attenuated N4CT1-gag1 vector that demonstrated safety and immunogenicity in 2 phase 1 clinical studies. The studies described here are the first to demonstrate protection of guinea pigs and macaques with a single dose of highly attenuated rVSV expressing EBOVGP, and we believe that the N4CT1-EBOVGP1 vector has the essential safety and efficacy characteristics for use in a vaccine to prevent EBOV infection in humans and the great apes. This highly attenuated vector platform will likely also have the potential to serve in a vaccine against other EBOV strains that cause disease in humans and apes. Ultimately, a multivalent EBOV vaccine may be possible by combining ≥2 vaccine vectors in a single vaccine formulation.

Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations

Serologic cross-reactivity of human IgM and IgG antibodies to five species of Ebola virus

Fruit bats as reservoirs of Ebola virus

Spatial and temporal patterns of Zaire ebolavirus antibody prevalence in the possible reservoir bat species

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Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor

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Vesicular stomatitis virus, Indiana serotype: multiplication in and transmission by experimentally infected phlebotomine sandflies (Lutzomyia trapidoi)

Vesicular stomatitis virus (Indiana serotype): transovarial transmission by phlebotomine sandflies

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In vivo biodistribution of a highly attenuated recombinant vesicular stomatitis virus expressing HIV-1 Gag following intramuscular, intranasal, or intravenous inoculation

Synergistic attenuation of vesicular stomatitis virus by combination of specific G gene truncations and N gene translocations

Guide for the care and use of laboratory animals

An efficient helper-virus-free method for rescue of recombinant paramyxoviruses and rhadoviruses from a cell line suitable for vaccine development

A simple method of estimating fifty percent endpoints

Pathogenesis of experimental Ebola virus infection in guinea pigs

Modifying the HIV-1 env gp160 gene to improve pDNA vaccine-elicited cell-mediated immune responses

Enzyme-linked immunosorbent assay for detection of filovirus species-specific antibodies

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Vesicular stomatitis virus-based vaccines protect nonhuman primates against aerosol challenge with Ebola and Marburg viruses

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Structure-function analysis of the soluble glycoprotein, sGP, of Ebola virus

Recombinant and virion-derived soluble and particulate immunogens for vaccination against tick-borne encephalitis

Ebola virus-like particles protect from lethal Ebola virus infection

Impact of Ebola mucin-like domain on antiglycoprotein antibody responses induced by Ebola virus-like particles

Influences of glycosylation on antigenicity, immunogenicity, and protective efficacy of Ebola virus GP DNA vaccines

Ebola virus entry requires the cholesterol transporter Niemann-Pick C1

Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines

Distinct cellular interactions of secreted and transmembrane Ebola virus glycoproteins

Transcriptional activation of alpha/beta interferon genes: interference by nonsegmented negative-strand RNA viruses

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Financial support. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
